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1篇 您的检索式:作者名="Nicolas Reynoud"
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1The SlSHN2 transcription factor contributes to cuticle formation and epidermal patterning in tomato fruit显示文摘Tomato(Solanum lycopersicum)is an established model for studying plant cuticle because of its thick cuticle covering and embedding the epidermal cells of the fruit.In this study,we screened an EMS mutant collection of the miniature tomato cultivar Micro-Tom for fruit cracking mutants and found a mutant displaying a glossy fruit phenotype.By using an established mapping-by-sequencing strategy,we identified the causal mutation in the SlSHN2 transcription factor that is specifically expressed in outer epidermis of growing fruit.The point mutation in the shn2 mutant intro-duces a K to N amino acid change in the highly conserved‘mm’domain of SHN proteins.The cuticle from shn2 fruit showed a~fivefold reduction in cutin while abundance and composition of waxes were barely affected.In addition to alterations in cuticle thickness and properties,epidermal patterning and polysaccharide composition of the cuticle were changed.RNAseq analysis further highlighted the altered expression of hundreds of genes in the fruit exocarp of shn2,including genes associated with cuticle and cell wall formation,hormone signaling and response,and transcrip-tional regulation.In conclusion,we showed that a point mutation in the transcriptional regulator SlSHN2 causes major changes in fruit cuticle formation and its coordination with epidermal patterning.Cecile Bres Johann Petit Nicolas Reynoud Lysiane Brocard Didier Marion Marc Lahaye Benedicte Bakan Christophe Rothan 2022Molecular Horticulture2022,2,1:0
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