|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG显示文摘Objective: To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis(M. tuberculosis) of a proteoliposome(PL)from Mycobacterium bovis Bacillus Calmette–Guerin(BCG) with and without alum hydroxide(AL) as adjuvant(PLBCG-AL and PLBCG, respectively) in BALB/c mice.Methods: BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G(Ig G), Ig G1 and Ig G2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot.Results: Sera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total Ig G and Ig G1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo.Conclusions: The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages. | Nadine Alvarez DaymíSerpa Ramlah Kadir Yanely Tirado Reinier Borrero Sonsire Fernández Rubén Cabrera Yolanda Valdes Caridad Zayas Reinaldo Acevedo Luis Izquierdo María Elena Sarmiento Mohd-Nor Norazmi JoséLuis Pérez Armando Acosta | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，3： | 3 |
| 2 | Role of toll like-receptor 2 in inflammatory activity of macrophage infected with a recombinant BCG expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum显示文摘Objective: To investigate the role of toll-like receptor 2(TLR2) in inflammatory activity of macrophage infected with the recombinant Mycobacterium bovis bacillus Calmette-Guerin(rBCG). Methods: Mouse macrophage cell line J774 A.1 was infected with Mycobacterium bovis bacillus Calmette-Guerin(BCG) and rBCG cultures for 48 h in the presence or absence of 10 μg/mL of TLR2 inhibitor. Untreated macrophages were used as a negative control while lipopolysaccharide-stimulated macrophages were used as a positive control. The ability of the macrophage to engulf the BCG and rBCG in the absence or presence of TLR2 inhibitor was assessed using a phagocytic assay, while the production of inflammatory cytokines and nitric oxide by the infected macrophages was evaluated using ELISA and Griess reagent method, while the expression of the inducible nitric oxide synthase was determined using Western blot analysis. Results: The results showed that blocking TLR2 function reduced the phagocytic activity, nitric oxide production and proinflammatory cytokine secretion such as TNF-α, IL-1β and IL-12 p40 as well as inducible nitric oxide synthase expression in the infected macrophages. These data showed the importance of TLR2 in the activation of macrophages following BCG and r BCG infections. Conclusions: Through exploring the immunological mechanism which underlies the protection conferred by the candidate vaccine, this study will improve our understanding of the vaccine candidate's mechanism to protect the host from malaria infection. | Nor Munirah Zakaria Rapeah Suppian Norazmi Mohd Nor Nor Fazila Che Mat | 2018 | Asian Pacific Journal of Tropical Biomedicine2018，8，7： | 2 |
| 3 | Pattern recognition receptors and cyto- kines in Mycobacterium tuberculosis infection-the double-Edged sword? 显示文摘 | Hossain MM Norazmi MN | 2013 | Biomed Res Int2013，2013，17： | 1 |
| 4 | Increased level of activated gamma delta lymphocytes correlates with disease severity in HIV infection显示文摘 | Norazmi MN Arifin H Jamaruddin MA | 1995 | Immunol Cell Biol1995，73，3： | 1 |
| 5 | Pattern recognition receptors and cytokines in mycobacterium tuberculosis infection-the double-edged sword?显示文摘 | Hossain MM Norazmi MN | 2013 | Biomed Res Int2013，2013，17： | 1 |
| 6 | Approaches towards the development of a vaccine against tuberculosis : re- combinant BCG and DNA vaccine显示文摘 | Norazmi Mohd Nor Mustaffa Musa | 2004 | Tuberculosis2004，，84： | 1 |
| 7 | Immunogenicity of re- combinant BCG-based vaecine expressing the 22 kDa of serine repeat antigen (SE22)of Plasmodium falciparum 显示文摘 | Teo WH Nurul AA Norazmi MN | 2012 | Trop Biomed2012，29，2： | 1 |
| 8 | Innnunogenicity of recombinant BCG-based vaccine expressing the 22kDa of serine repeat antigen (SE22) of Plasmodium falciparum显示文摘 | Teo WH Nurul AA Norazmi MN | | 0，，2： | 1 |
| 9 | Immunogenicity and in vitro protective efficacy of recombinant Mycobacterium bovis bacillus CalmetteGuerin (rBCG) expressing the 19kDa merozoite surface protein (MSP-1 (19显示文摘 | Nurul AA Norazmi MN | | 0，，4： | 1 |
| 10 | Modulation of cell growth and PPARgamma expression in human colorectal cancer cell lines by ciglitazone 显示文摘 | Yaacob NS Dams HM Norazmi MN | 2008 | Exp Toxicol Pathol2008，60，6： | 1 |
| 11 | Pattern Recognition Receptors and Cytokines in Mycobacterium tuberculosis Infection—The Double-Edged Sword?显示文摘 | Md. Murad Hossain Mohd-Nor Norazmi Gernot Zissel | 2013 | BioMed Research International2013，，： | 1 |
| 12 | Immunogenicity of a recombinant Mycobacterium bovis bacille Calmette-Guerin expressing malarial and tuberculosis epitopes显示文摘 | Rapeah S Norazmi MN | 2006 | Vaccine2006，24，17： | 1 |
| 13 | Liposomes as immunological adjuvants and delivery systems in the development of tuberculosis vaccine:A review显示文摘Liposomes are phospholipid bilayer vesicles,which are biocompatible,biodegradable and nontoxic vehicles suitable for numerous drug and gene delivery applications.In this review,we discuss the prospect of using liposome technology in the development of a vaccine for tuberculosis.Tuberculosis remains an important health problem that requires the development of an effective vaccine,especially since the only approved vaccine for it continues to be the Bacille Calmette-Geurin(BCG)one developed 100 years ago.This review focuses on the different applications of liposomes toward achieving this goal.Numerous liposomal formulations showing prospect in the research stage and in clinical trials are discussed. | Nur Ellene Mat Luwi Suhana Ahmad Ahmad Suhaimi Nurfatihah Azlyna Asyikin Nordin Maria Elena Sarmiento Armando Acosta Mohd Nor Norazmi Vuk Uskoković Rohimah Mohamud Ramlah Kadir | 2022 | Asian Pacific Journal of Tropical Medicine2022，15，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员