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| 1 | Evaluation of clinical relevance of examining K-ras, p16 and p53 mutations along with allelic losses at 9p and 18q in EUS-guided fine needle aspiration samples of patients with chronic pancreatitis and pancreatic cancer显示文摘AIM: To establish an optimum combination of molecular markers resulting in best overall diagnostic sensitivity and specificity for evaluation of suspicious pancreatic mass. METHODS: Endoscopic ultrasound (EUS)-guided fine needle aspiration cytology (FNA) was performed on 101 consecutive patients (63 males, 38 females, 60 ± 12 years; 81 with subsequently diagnosed pancreatic cancer, 20 with chronic pancreatitis) with focal pancreatic mass. Samples were evaluated on-site by an experienced cytopathologist. DNA was extracted from Giemsa stained cells selected by laser microdissection and the presence of K-ras, p53 and p16 somatic mutations was tested by cycling-gradient capillary electrophoresis (CGCE) and single-strand conformation polymorphism (SSCP) techniques. In addition, allelic losses of tumor suppressor genes p16 (INK4, CDKN2A) and DPC4 (MADH4, SMAD4) were detected by monitoring the loss of heterozygosity (LOH) at 9p and 18q, respectively. RESULTS: Sensitivity and specificity of EUS-guided FNA were 75% and 85%, positive and negative predictivevalue reached 100%. The remaining 26% samples were assigned as inconclusive. Testing of molecular markers revealed sensitivity and specificity of 70% and 100% for K-ras mutations (P < 0.001), 24% and 90% for p53 mutations (NS), 13% and 100% for p16 mutations (NS), 85% and 64% for allelic losses at 9p (P < 0.001) and 78% and 57% for allelic losses at 18q (P < 0.05). When tests for different molecular markers were combined, the best results were obtained with K-ras + LOH at 9p (92% and 64%, P < 0.001), K-ras + LOH at 18q (92% and 57%, P < 0.001), and K-ras + LOH 9q + LOH 18q (96% and 43%, P < 0.001). When the molecular markers were used as complements to FNA cytology to evaluate inconclusive samples only, the overall sensitivity of cancer detection was 100% in all patients enrolled in the study. CONCLUSION: EUS-guided FNA cytology combined with screening of K-ras mutations and allelic losses of tumor suppressors p16 and DPC4 represents a very sensitive approach in screening for pancreatic malignancy. Molecular markers may find its use particularly in cases where FNA cytology has been inconclusive. | C Salek L Benesova M Zavoral V Nosek L Kasperova M Ryska R Strnad E Traboulsi M Minarik | 2007 | World Journal of Gastroenterology2007，13，27： | 18 |
| 2 | Natural orifice transluminal endoscopic surgeryvs laparoscopic ovariectomy:Complications and inflammatory response显示文摘AIM:To compare natural orifice transluminal endoscopic surgery (NOTES) vs standard laparoscopic ovariectomy in mini pigs with respect to technical aspects,complications and parameters of systemic inflammatory response.METHODS:This was a randomized,experimental,survival study.Ten female mini pigs underwent NOTES transgastric ovariectomy (NOTES group) and ten female mini pigs underwent laparoscopic ovariectomy (LAP group).A 'percutaneous endoscopic gastrostomy' approach with guidewire and sphincterotome was used for gastrotomy creation.The ovary was resected using standard biopsy forceps and a snare.The access site was closed using a 'KING' closure with a single endoloop and several clips.In the laparoscopic group,a three-port laparoscopy and an ovariectomy were performed with the use of standard laparoscopic devices.C-reactive protein (CRP),white blood count and interleukin (IL)-6 plasma levels were used as indicators of systemic inflammatory response.All animals were euthanized 28 d after surgery.RESULTS:All animals survived without complications.The mean procedure time was 41.3 min ± 17.6 min (NOTES group) and 25.7 min ± 5.25 min (LAP group,P < 0.02).Postmortem examinations demonstrated that 50% and 70% of animals were free of any complications in the NOTES and LAP groups,respectively.The remaining animals developed minor complications (adhesions) in a comparable frequency between the two groups.In the NOTES group,one animal developed a small intramural gastric abscess close to the gastrotomy site.A minor serous exudate that was present in 50% and 40% of the animals in the NOTES and laparoscopy groups,respectively,was not considered a complication.In both groups CRP levels increased significantly on the 2nd and 7th postoperative days (POD) and returned to normal after 28 d.On POD 2,an increase of CRP level was significantly higher in the NOTES group compared to the LAP group.Values of IL-6 did not differ from baseline values in either of the groups postoperatively.Interestingly,the platelet count decreased significantly on POD 2,but returned close to baseline values on POD 7 and PODs 28-30.CONCLUSION:Both NOTES and laparoscopic ovariectomies had a similar frequency of minor complications.However,the NOTES technique produced an increased systemic inflammatory response on POD 2. | Jan Martínek Ondej Ryska Tereza Filípková Radek Dolezel Stefan Juhas Jan Motlík Monika Holubová Vladimír Nosek Barbora Rotnáglová Miroslav Zavoral Miroslav Ryska | 2012 | World Journal of Gastroenterology2012，18，27： | 4 |
| 3 | Understanding and using the implicit association test : An improved scoring algorithm显示文摘 | Greenwald A G Nosek B A Banaji M R | 2003 | Journal of Personality and Social Psychology2003，85，2： | 1 |
| 4 | Health promotion for women with physical disabilities: a pilot study 显示文摘 | Hughes RB Nosek MA Howland CA | 2003 | Rehabil Psychol2003，48，3： | 1 |
| 5 | Absorption and metabolic effect of inhaled insulin: intrapatient variability after inhalation via the Aerodose insulin inhaler in patients with type 2 diabetes 显示文摘 | Perera AD Kapitza C Nosek L | 2002 | Diabetes Care2002，25，12： | 1 |
| 6 | Lower within-subject variability of insulin detemir in comparison to NPH insulin and insulin glargine in people with type 1 diabetes显示文摘 | Heise T Nosek L Ronn BB | 2004 | Diabetes2004，53，6： | 1 |
| 7 | Changes in basal insulin infusion: time until a change in metabolic effect is induced in patients with type 1 diabetes 显示文摘 | Heinemann L Nosek L Kapitza C | 2009 | Diabetes Care2009，32，8： | 1 |
| 8 | How valproate and phenytoin affect the ionic conductances and active transport characteristics of the crayfish giant axon显示文摘 | Nosek TM | 1981 | Epilepsia1981，22，： | 1 |
| 9 | Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy显示文摘 | Jin JP Brotto MA Hossain MM Huang QQ Brotto LS Nosek TM | 2003 | J Biol Chem2003，278，26： | 1 |
| 10 | Understanding and using the implicit association test:An Improved Scoring Algorithm显示文摘 | Greenwald A G Nosek B A Banaji M R | 2003 | Journal of Personality and Social Psychology2003，85，2： | 1 |
| 11 | No Place for Nostalgia in Science: A Response to Arkes and Tetlock 显示文摘 | Banaji M R Nosek B A Greenwald A G | 2004 | Psychological Inquiry2004，，15： | 1 |
| 12 | Superoxide,hydroxyl raqdical,and hydrogen peroxide effects on single-diaphragm fiber contraction apparatus显示文摘 | Callahan LA She ZW and Nosek TM | 2001 | J Appl Physiol2001，90，1： | 1 |
| 13 | High-efficiency Transformation of the Pathogenic Yeast Candida parapsilosis 显示文摘 | ZEMANOVA J NOSEK J TOMASKA L | 2004 | CurrGenet2004，45，3： | 1 |
| 14 | Affective focus increases the concordance between implicit and explicit attitudes显示文摘 | Smith CT Nosek BA | 2011 | Soc Psychol2011，42，： | 1 |
| 15 | Contextual variations in implicit evaluation显示文摘 | Mitchell JP Nosek BA Banaji MR | | 0，，： | 1 |
| 16 | Lower within-subject variability of insulin detemir in comparison to NPH insulin and insulin glargine in people with type 1 diabetes显示文摘 | Heise T Nosek L Ronn B | 2004 | Diabetes2004，53，6： | 1 |
| 17 | Lower within-subject variability of insulin detemir in comparison to NPH insulin and insulin glargine in people with type 1 diabetes显示文摘 | Heise T Nosek L Ronn BB | 2004 | Diabetes2004，53，6： | 1 |
| 18 | Understanding and Using the Implicit Association Test Ⅰ an Improved Scoring Algorithm 显示文摘 | Greenwald A G Nosek B A Banaji M R | 2003 | Journal of Personality and Social Psychology2003，85，2： | 1 |
| 19 | Insulin degludec: less pharmacodynamic variability than insulin glargine under steady state conditions显示文摘 | Heise T Hermanski L Nosek I | 2010 | Diabetologia2010，53，1： | 1 |
| 20 | Lower within-subject variability of insulin detemir in comparison to NPH in- sulin and insulin glargine in people with type I diabetes 显示文摘 | Heise T Nosek L Ronn BB | 2004 | Diabetes2004，53，6： | 1 |
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