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| 1 | Effects of suppressing glucose transporter-1 by an antisense oligodeoxynucleotide on the growth of human hepatocellular carcinoma cells显示文摘BACKGROUND:The glucose transporter-1(Glut-1),a key ratelimiting factor in the transport and metabolism of glucose in cancer cells,is over-expressed in many human cancer cells and this overexpression is correlated with poor biological behavior. The increased levels of Glut-1 expression in hepatocellular carcinoma(HCC)cells functionally affect tumorigenicity.This study was undertaken to investigate effects of suppressing Glut-1 by an antisense oligodeoxynucleotide(AS-ODN)on the growth of human hepatocellular carcinoma(HepG-2)cells. METHODS:We used AS-ODN targeting against the Glut-1 gene in a HepG-2 cell line.There were four experimental groups: empty pcDNA3.1 vector(mock transfection),pcDNA3.1-anti-Glut(+),pcDNA3.1-Glut(+),and non-transfected HepG-2 cells. The Glut-1 mRNA expression was detected by RT-PCR and the Glut-1 protein expression by Western blotting after cell culture, and the glucose uptake was detected after glucose stimulation in each group. RESULTS:Compared with non-transfected HepG-2 or Glut-1 pcDNA3.1,a down-regulation of Glut-1 mRNA in HepG-2 cells transfected with anti-Glut-1 pcDNA3.1 was noted(P<0.05).Glut-1 protein in HepG-2 cells transfected with Glut-1 AS-ODN was decreased compared with non-transfected HepG-2,Glut-1 pcDNA3.1,or empty vectors. Glucose uptake by the HepG-2 cells transfected with AS-ODN was decreased at 1 hour after glucose stimulation.CONCLUSIONS:The application of Glut-1 AS-ODN can down-regulate the expression of Glut-1 at mRNA and protein,and inhibit glucose uptake partially in HepG-2 cells.The Glut-1 gene maybe a potential therapeutic target for HCC. | Tian-Qi Liu,Jun Fan,Lin Zhou and Shu-Sen Zheng Key Laboratory of Combined Multi-organ Trans-plantation,Ministry of Public Health Key Laboratory of Organ Trans-plantation,Zhejiang Province and Division of Hepatobiliary and Pancreatic Surgery,Department of Surgery State Key Laboratory for Diagnosis and Treatment of Infectious Disease,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China Department of Hepatobiliary Surgery,the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，1： | 9 |
| 2 | Establishment and evaluation of the system of extracorporeal liver perfusion in pigs显示文摘BACKGROUND; In recent years, extracorporeal liver per- fusion (ECLP) has been regarded as a treatment of acute liver failure ( ALF ); but the system of ECLP has many problems. The purpose of this experiment was to detect the factors affecting the system of ECLP and to establish a sta- ble and effective system of ECLP. METHODS; Livers were harvested from health pigs, ac- cording to the different styles of perfusion and oxygena- tion, which were randomly divided into 3 groups. The liv- ers in group A (n =4) were subjected to single portal vein perfusion, oxygenating perfusion blood; the livers in group B (n =4) to dual ( portal vein and hepatic artery) vessel perfusion, oxygenating blood, together; and the livers in group C ( n = 4) to dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood, separately. The perfusion time, the data of bile production, and hemody- namic parameters of extracorporeal livers in each group were tested. The histological examination of liver tissues from each group was performed at the end of perfusion. RESULTS: The perfusion time of the liver in group A is significantly shorter than in groups B and C (P<0.05). At 1 , 3 , 6 hours after perfusion, the data of bile production and hemodynamic parameters of livers in group A were sta- tistically different from those of livers in groups B and C (P<0.05). At 1, 3, 6 hours after perfusion, the data of group B were not statistically different from those of livers in group C (P>0.05). But at 12 hours after perfusion, the data of group B were statistically different from those of liv- ers in group C (P<0.01). CONCLUSION: The system of ECLP, which is performed by dual ( portal vein and hepatic artery) vessel perfusionand oxygenating blood separately, is more stable and effec- tive to keep the function of extracorporeal liver. | Jin Gong, Xi-Mo Wang, Gang Long, Zhong-Tao Guo, Tao Jiang and Shi Chen Wuhan, ChinaInstitute of Organ Transplantation, Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430030, China Department of Surgery, Tianjin People’s Hospital, Tianjin 300120, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 7 |
| 3 | Rate minimum in solution crystallization of long paraffins 显示文摘 | ORGAN S J UNGAR G KELLER A | 1989 | Macromolecules1989，，22： | 1 |
| 4 | Characteristics of aerobic granules 显示文摘 | Tay J H Pan S He Y X et aL Effect of organic loading rate on aerobic granulation: Part II | 2004 | J Environ Eng2004，130，： | 1 |
| 5 | Chemical inhibition of acetyl-CoA carboxylase induces growth arrest and cytotoxicity selectively in cancer cells 显示文摘 | Beckers A Organe S Timmermans L | 2007 | Cancer Res2007，67，17： | 1 |
| 6 | Job satisfaction and the good soldier: The relationship between affect and em- ployee 'citizenship'显示文摘 | Bateman T S Organ D W | 1983 | Academy of Management Jour- nal1983，26，4： | 1 |
| 7 | Job satisfaction and the good soldier:the relationship between affect and employee citizenship显示文摘 | Bateman T S Organ D W | 1983 | Academy of Management Journal1983，26，： | 1 |
| 8 | Job satisfaction and the good soldier:The relationship between affect and employee 'citizenship'显示文摘 | BATEMAN T S ORGAN D W | 1983 | Academy of Management Journal1983，26，： | 1 |
| 9 | Comparison of fondaparinuxand enoxaparin in ^cute coronary syndromes 显示文摘 | Fifth Organization to Assess Strategies in Acute Ischemic SyndromesInvestigators Yusuf S Mehta SR | 2006 | N Engl J Med2006，354，14： | 1 |
| 10 | Chemical inhi- bition of acetyl-CoA carboxylase induces growth arrest and cytotoxicity selectively in cancer cells 显示文摘 | Beckers A Organe S Timmermans L | 2007 | Cancer Res2007，67，17： | 1 |
| 11 | Correlation between defensin expression and induction profiles in gingival keratinocytes显示文摘 | Joly S Organ CC Johnson GK | 2005 | Mol Immunol2005，42，9： | 1 |
| 12 | Job satisfaction and the good soldier:Tile relationship between affect and employee, citizenship显示文摘 | Bateman T S Organ D W | 1983 | Academy of Management Journal1983，，26： | 1 |
| 13 | Job satisfaction and the good soldier: the relationship between affect and employee 'citizenship' 显示文摘 | Bateman T S Organ D W | 1983 | Academy of Management Journal1983，26，4： | 1 |
| 14 | The C om m itm en t-tru st T h eoryo f R elation sh ip M arketin g显示文摘 | M organ R M H unt S D | 1994 | Jou rn al o f M arketing1994，58，3： | 1 |
| 15 | Dynamics of crop yields and carbon in a long-term fertilization the Huang-Huai-Hai Plain of China 显示文摘 | Cai Z C @in soil organic experiment in S W | 2006 | Geoderma2006，136，： | 1 |
| 16 | Job Satisfaction and the Good Soldier:The Relationship between Affect and Employee Citizenship显示文摘 | BATEMAN T S ORGAN D W | 1983 | Academy of Management Journal1983，，26： | 1 |
| 17 | European evidence-based consensus on the diagnosis and management of ulcerative colitis显示文摘 | The European Crohn′s and Colitis Organization | 2008 | J Crohn′s Colitis2008，2，1： | 1 |
| 18 | Syndesmosis fixation: a comparison of three and four cortices of screw fixation without hardware removal显示文摘 | Moore J A Jr Shank J R M organ S J | 2006 | Foot Ankle Int2006，27，8： | 1 |
| 19 | Chemical inhibition of acetyl-CoA carboxylase induces growth arrest and cytotoxicity selectively in cancer cells显示文摘 | Beckers A Organe S Timmermans L | 2007 | Cancer Res2007，67，17： | 1 |
| 20 | Active gate control of series connected IGBTs using positive current feedback technique显示文摘 | Krishna D Agarwal V Organ I S R | 2005 | IEEE Transactions on Circuits and Systems lI: Express Briefs2005，52，5： | 1 |
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