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| 1 | YKL40 expression in CD14^+ liver cells in acute and chronic injury显示文摘AIM:To demonstrate that CD14 + cells are an important source of the growth factor YKL40 in acute and chronic liver damage.METHODS:Rats were inoculated with one dose of CCl4 to induce acute damage.Liver biopsies were obtained at 0,6,12,24,48 and 72 h.For chronic damage,CCl4 was administered three days per week for 6 or 8 wk.Tissue samples were collected,and cellular populations were isolated by liver digestion and purified by cell sorting.YKL40 mRNA and protein expression were evaluated by realtime polymerase chain reaction and western blot.RESULTS:Acute liver damage induced a rapid increase of YKL40 mRNA beginning at 12 h.Expression peaked at 24 h,with a 26fold increase over basal levels.By 72 h however,YKL40 expression levels had nearly returned to control levels.On the other hand,chronic damage induced a sustained increase in YKL40 expression,with 7and 9fold higher levels at 6 and 8 wk,respectively.The pattern of YKL40 expression in different subpopulations showed that CD14+cells,which include Kupffer cells,are a source of YKL40 after acute damage at 72 h[0.09 relative expression units(REU)]as well as after chronic injury at 6 wk(0.11 REU).Hepatocytes,in turn,accounted for 0.06 and 0.01 REU after 72 h(acute)or 6 wk(chronic),respectively.The rest of the CD14cells(including T lymphocytes,B lymphocytes,natural killer and natural killer T cells) yielded 0.07 and 0.15 REU at 72 h and 6 wk,respectively.YKL40 protein expression in liver was detected at 72 h as well as 6 and 8 wk,with the highest expression relative to controls(11fold;P≤0.05)seen at 6 wk.Macrophages were stimulated by lipopolysaccharide.We demonstrate that under these conditions,these cells showed maximum expression of YKL40 at 12 h,with P<0.05 compared with controls.CONCLUSION:Hepatic CD14 + cells are an YKL40 mRNA and protein source in acute and chronic liver injury,with expression patterns similar to growth factors implicated in inflammationfibrogenesis. | Oscar Pizano-Martínez Irinea Yaez-Sánchez Pilar Alatorre-Carranza Alejandra Miranda-Díaz Pablo C Ortiz-Lazareno Trinidad García-Iglesias Adrian Daneri-Navarro Mónica Vázquez-Del Mercado Mary Fafutis-Morris Vidal Delgado-Rizo | 2011 | World Journal of Gastroenterology2011，17，33： | 3 |
| 2 | Insulin resistance impairs sustained response rate to peginterferon plus ribavirin in chronic hepatitis C patients显示文摘 | Manuel Romero-Gómez Maria Del Mar Viloria Raúl J. Andrade Javier Salmerón Moisés Diago Conrado M. Fernández-Rodríguez Raquel Corpas Marina Cruz Lourdes Grande Luis Vázquez Paloma Mu?oz-de-Rueda Pilar López-Serrano Ana Gila María L. Gutiérrez Celia Pérez A | 2005 | Gastroenterology2005，，3： | 2 |
| 3 | Chitosan and Chitosan/ethylene oxide-propylene oxide block copolymer nanoparticles as noval carriers for proteins and vaccines显示文摘 | PILAR C CARMEN R L JOSE LUIS V J | 1997 | Pharm Res1997，14，10： | 1 |
| 4 | Determination of Chloramphenicol Residues in Animal Feeds by Liquid Chromatography with Photo-diode Array Detection, Manuel Hempandez Cpordoba 显示文摘 | PILAR V NURIA B | 2006 | Analytica Chimica Acta2006，558，： | 1 |
| 5 | The effect of financial constraints, absorptive capacity and complementarities on the adoption of multiple process technologies显示文摘 | JAIME G PILAR V | 2009 | Research Policy2009，，38： | 1 |
| 6 | Therapeutic hypotherrnia : past, pres- ent, and future 显示文摘 | Joseph V FCCP Pilar A | 2008 | Chest2008，133，5： | 1 |
| 7 | Preparation of pure hectorite using microwaves显示文摘 | ISABEL V PILAR S YOLANDA C | 2010 | Physics Procedia2010，8，： | 1 |
| 8 | Metabolism of oak leaf ellagitannins and urolitbin production in beef cattle 显示文摘 | Rocia G B Pilar T Rocio G V | 2012 | J Agric Food Chem2012，86，： | 1 |
| 9 | Congenital and acquired anomalies of the portal venous systerm显示文摘 | Carmen G Maria V Pilar M | 2002 | Radiographics2002，22，1： | 1 |
| 10 | Stir Bar Sorptive Extraction Coupled to Liquid Chromatography for the Analysis of Strobilurin Fungicides in Fruit Samples 显示文摘 | NATALIA C PILAR V NEREA A | 2010 | Journal of Chromatography A2010，，1217： | 1 |
| 11 | Reversed-phase liquid chromatography on an amide stationary phase for the determination of the B group vitamins in baby foods显示文摘 | Pilar V Carmenl E Nuri A B | 2003 | Chromatogr A2003，1007，： | 1 |
| 12 | Nicotinamide induces differentiation of embryonic stem cells into insulin-secreting cells 显示文摘 | Pilar V Genoveva B Raquel A | 2008 | Exp Cell Res2008，314，5： | 1 |
| 13 | Occurrence of toxic cyanobacterial blooms in San Roque Reservoir (Cordoba,Argentina): a field and chemometric study显示文摘 | Ame M V del Pilar Diaz M Wunderlin D A | 2003 | Environmental Toxicology2003，18，3： | 1 |
| 14 | Life cycle assessment of composite ma- terials made of recycled thermoplastics combined with rice husks and cotton linters 显示文摘 | Rosario V Pilar M Daniel G | 2009 | The International Journal of Life Cycle Assessment2009，14，1： | 1 |
| 15 | Organizational learning capability: a proposal of measurement 显示文摘 | Pilar J G Jose C L Ramo V C | 2005 | Journal of Business Research2005，58，6： | 1 |
| 16 | Reversed-phase liquid chromatography on an amide stationary phase for the determination of the B group vitamins in baby foods显示文摘 | PILAR V CARMEN L E NURIA B | 2003 | J Chromatogr: A2003，1007，： | 1 |
| 17 | Fractionated stereotactic radiotherapy in the treatment of exclusive cavernous sinus meningioma:functional outcome,local control,and tolerance显示文摘 | Marta B Salvador V Pilar T | | 0，，： | 1 |
| 18 | Method development and validation for strbilurin fungicides in baby foods by solid-phase microextraction gas chromatography-mass spectrometry显示文摘 | PILAR V NATALIA C NELSON M C | 2009 | Journal of Chromatography A2009，，1216： | 1 |
| 19 | Viability PCR, a Culture-Independent Method for Rapid and Selective Quantification of Viable Legionella pneumophila Cells in Environmental Water Samples显示文摘 | Pilar D V Lydie S Delattre J M | 2010 | Applied and Environmental Microbiology2010，6，15： | 1 |
| 20 | Organizational learning capability:A proposal of measurement显示文摘 | Pilar J G Jose C L Ramon V C | 2005 | Journal of Business Research2005，58，7： | 1 |
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