|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 新致病基因在颅缝早闭Crouzon综合征中的初步机制研究显示文摘目的研究Rbp4(Retinol binding protein 4)、Gpc3(Glypican family of growth factor binding protein 3)、C1qtnf3(Collagenous repeat-containing sequence of 26 KDa protein)等新致病基因在颅缝早闭症中的发病机制,为疾病非手术治疗奠定理论基础。方法以Crouzon综合征Fgfr2cC342Y/+小鼠为实验模型,应用MicroCT和组织学染色,研究小鼠颅缝闭合模式;以Fgfr2cC342Y/+模型,RT-qPCR研究Rbp4、Gpc3、C1qtnf3等新致病基因的表达差异,初步探讨其在颅缝闭合过程中的调控作用;以体外培养的Fgfr2cC342Y/+小鼠颅缝细胞为模型,研究基因突变动物细胞增殖与代谢改变。结果获得Fgfr2cC342Y/+小鼠后额缝、冠状缝、人字缝、矢状缝等颅缝闭合模式,随颅骨发育、颅缝闭合,OC(Osteocalcin)、ALP(Alkalinephosphatase)表达增加,目的基因Rbp4、Gpc3、C1qtnf3表达下降,Msx2(Muscle segment homeobox gene 2)表达增加,杂合子与野生型小鼠之间均存在显著统计学差异,与前期人颅缝组织Microarray研究结果一致。Gpc1(Glypican family ofgrowth factor binding protein 1)、FliI(Flightless I)在颅缝闭合中的表达较恒定,野生型与杂合子之间未见明显统计学差异。体外培养Fgfr2cC342Y/+小鼠颅缝细胞,CellTiter96 MTS和Quant-iT Picogreen dsDNA细胞增殖与代谢分析结果显示,Fgfr2功能获得性突变可促进冠状缝细胞的增殖,从而导致成骨增加,颅缝早闭。结论 Fgfr2cC342Y/+模型新致病基因表达趋势与前期人颅缝组织Microarray研究结果一致,Rbp4、Gpc3、C1qtnf3可能在颅缝早闭中具重要调控作用。 | 杨娴娴 Jodie T Hatfield Susan J Hinze 穆雄铮 Peter J Anderson Barry C Powell | 2012 | 组织工程与重建外科杂志2012，8，5： | 3 |
| 2 | Organizational alignment as competitive advantage显示文摘 | Powell T C | 1992 | Strategie Management Journal1992，13，2： | 1 |
| 3 | A double blind, randomized, controlled trial of glutamine supplementation in parenteral nutrition显示文摘 | Powell T J Jamieson C P Bettany G E | 1999 | Gut1999，45，1： | 1 |
| 4 | Laparoscopic renal cryotherapy: biology, techniques and outcomes 显示文摘 | POWELL T WHELAN C SCHWARTZ B F | 2005 | Minerva Urol Nefrol2005，57，2： | 1 |
| 5 | Post-traumatic growth 'after head injury:a long-term follow-up 显示文摘 | Powell T Ekin-Wood A Collin C | 2007 | Brain lnj2007，21，1： | 1 |
| 6 | Influence of tempera- ture and rainfall on the development of tobacco black shank 显示文摘 | Jacobi W R Main C E Powell N T | 1983 | Phytopathology1983，73，2： | 1 |
| 7 | Information Tehcnology as Competitive Advantage: The Role of Human, Business and Technology Resources 显示文摘 | Powell T C Micallef-Dent A | 1997 | Strategic Management Journal1997，18，5： | 1 |
| 8 | Efficacy and Safety of a Low Molecular Weight Hyaluronic Acid Topical Gel in the Treatment of Facial Seborrheic Dermatitis Final Report 显示文摘 | Schlesinger T Powell C R | 2014 | The Journal of clinical and aesthetic dermatology2014，7，5： | 1 |
| 9 | Pancreaticoduodenectomy : a 20-year experience in 516 patients显示文摘 | Schmidt C M Powell E S Yiannoutsos C T | 2004 | Arch Surg2004，139，7： | 1 |
| 10 | Administration of a CD25 - directed immunotoxin, LMB-2, to patients with metastatic melanoma induces a selective partial reduction in regulatory T cells in vivo 显示文摘 | Powell DJ Jr Felipe-Silva A Merino M J Ahmadzadeh M Alien T Levy C | 2007 | J Immunol2007，179，49： | 1 |
| 11 | Information Technology as Competitive Advantage: The Role of Human, Business, and Technology Resources 显示文摘 | POWELL T C DENT MICALLEF A | 1997 | Strategic Management Journal1997，18，5： | 1 |
| 12 | Total quality management as competitive advantage:a review and empirical study显示文摘 | POWELL T C | 1995 | Strategic Management Journal Chichester1995，16，1： | 1 |
| 13 | Complaint voicing by industrial buyers 显示文摘 | Barksdale H C Powell T E and Hargrove E | 1984 | Industrial Marketing Mnagement1984，，13： | 1 |
| 14 | a 20-year experience in 516 patients显示文摘 | Schmidt C M Powell E S Yiannoutsos C T | 2004 | Arch Surg2004，139，7： | 1 |
| 15 | Molecular cloning of a conrplementary DNA encoding a prostate-specific membrane antigen 显示文摘 | Israeli P S Powell C T Fair W R etal | 1993 | Cancer Res1993，53，2： | 1 |
| 16 | A new clathrate hydrate structure 显示文摘 | Ripmeester J A John S T Ratcliffe C I Powell B M | 1987 | Nature1987，325，6100： | 1 |
| 17 | Transient aqueous pores in bilayer mem- branes: a statistical theory显示文摘 | Powell K T Weaver J C | 1986 | Bioelectrochemistry and Bioanergetics1986，15，2： | 1 |
| 18 | Information Technology As Competitive Advantage: The Role of Human, Business, and Technology Resources 显示文摘 | Powell T C Dent - Micallef A | 1997 | Strategic Management Journal1997，18，5： | 1 |
| 19 | Information technology as competitive advantage : The role of human, business, and technology resources 显示文摘 | Powell T C Dent-Micallef A | 1997 | Strategic Management Journal1997，18，5： | 1 |
| 20 | Influence of temperature and rainfall on the development of tobacco black shank 显示文摘 | Jacobi W R Main C E Powell N T | 1983 | Phytopathology1983，73，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员