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| 1 | A potato late blight resistance gene protects against multiple Phytophthora species by recognizing a broadly conserved RXLR-WY effector显示文摘Species of the genus Phytophthora,the plant killer,cause disease and reduce yields in many crop plants.Although many Resistance to Phytophthora infestans(Rpi)genes effective against potato late blight have been cloned,few have been cloned against other Phytophthora species.Most Rpi genes encode nucleotide-binding domain,leucine-rich repeat-containing(NLR)immune receptor proteins that recognize RXLR(Arg-X-Leu-Arg)effectors.However,whether NLR proteins can recognize RXLR effectors from multiple Phytophthora species has rarely been investigated.Here,we identified a new RXLR-WY effector AVRamr3 from P.infestans that is recognized by Rpi-amr3 from a wild Solanaceae species Solanum americanum.Rpi-amr3 associates with AVRamr3 in planta.AVRamr3 is broadly conserved in many different Phytophthora species,and the recognition of AVRamr3 homologs by Rpi-amr3 activates resistance against multiple Phytophthora pathogens,including the tobacco black shank disease and cacao black pod disease pathogens P.parasitica and P.palmivora.Rpi-amr3 is thus the first characterized resistance gene that acts against P.parasitica or P.palmivora.These findings suggest a novel path to redeploy known R genes against different important plant pathogens. | Xiao Lin Andrea Olave-Achury Robert Heal Marina Pais Kamil Witek Hee-Kyung Ahn He Zhao Shivani Bhanvadia Hari S.Karki Tianqiao Song Chih-hang Wu Hiroaki Adachi Sophien Kamoun Vivianne G.A.A.Vleeshouwers Jonathan D.G.Jones | 2022 | Molecular Plant2022，15，9： | 2 |
| 2 | STX5 Inhibits Hepatocellular Carcinoma Adhesion and Promotes Metastasis by Regulating the PI3K/mTOR Pathway显示文摘Background and Aims:Syntaxin 5(STX5)is a member of the syntaxin or target-soluble SNAP receptor(t-SNARE)fam-ily and plays a critical role in autophagy.However,its function and molecular mechanism in tumor cell migration are still un-known.The role of STX5 in influencing hepatocellular carci-noma(HCC)is an important topic in our research.Methods:By using quantitative reverse transcription polymerase chain reaction(qPCR),western blotting,and immunohistochemical analysis of RNA and protein in tissues,we comprehensively evaluated data sets from public databases and clinical patient cohorts for STX5.The correlation of STX5 expression with the clinicopathological characteristics of HCC patients were assessed.In addition,we predicted signal pathways from dif-ferentially expressed genes(DEGs)and the Cancer Genome Atlas(TCGA)databases,and confirmed the prediction using integrated transcriptome and RNA-seq.We further investi-gated the underlying mechanisms of STX5 in the migration and adhesion of HCC cells both in vitro and in vivo.Results:In the TCGA dataset and our patient cohort,STX5 levels were significantly higher in HCC tissues than in adjacent normal liver tissues.At the same time,high expression of STX5 pre-dicted worse prognosis in patients with liver cancer.High ex-pression of STX5 indicates the decrease of adhesion and the increase of migration of HCC cells,and the conversion of epi-thelial-mesenchymal transition(EMT)in vitro via PI3K/mTOR pathway activation.Conversely,when Sirolimus,a phospho-inositide 3-kinase(PI3K)/AKT/mechanistic target of rapa-mycin(mTOR)inhibitor acts on cells simultaneously,STX5 overexpression-mediated enhancement of HCC metastasis is reversed.Double-negative regulation of STX5 and mTOR further enhanced the inhibitory effect of STX5 on HCC me-tastasis.In vivo,STX5 knockdown inhibited the metastasis of HCC cells.Conclusions:Our study demonstrates a novel research result that STX5 promotes HCC metastasis through PI3K/mTOR pathway.We believe that combined inhibition of STX5 and mTOR is a potential treatment for effectively pro-longing patient survival and inhibiting HCC metastasis. | Bin Zhang Ziyin Zhao Youpeng Wang Tingting Guo Mingyang He Ge Guan Pai Peng Jinzhen Cai Bingyuan Zhang Xutao Liu Qiaoling Song | 2023 | Journal of Clinical and Translational Hepatology2023，11，3： | 1 |
| 3 | Region-of-interest micro-focus computed tomography based on an all-optical inverse Compton scattering source显示文摘Micro-focus computed tomography(CT),which allows the hyperfine structure within objects to be reconstructed,is a powerful nondestructive testing tool in many fields.However,current x-ray sources for micro-focus CT are typically limited by their relatively low photon energy and low flux.An all-optical inverse Compton scattering source(AOCS)based on laser wakefield acceleration can generate intense quasi-monoenergetic x/gamma-ray pulses in the kilo-to megaelectronvolt range with micrometer-level source size,and its potential application for micro-focus CT has become very attractive in recent years because of the rapid progress made in laser wakefield acceleration.Reported here is a successful experimental demonstration of high-fidelity micro-focus CT using an AOCS(∼70 keV)by imaging and reconstructing a test object with complex inner structures.A region-of-interest CT method is adopted to utilize the relatively small field of view of the AOCS to ensure high spatial resolution.This demonstration of AOCS-based region-of-interest micro-focus CT is a key step toward its application in the field of hyperfine nondestructive testing. | Yue Ma Jianfei Hua Dexiang Liu Yunxiao He Tianliang Zhang Jiucheng Chen Fan Yang Xiaonan Ning Zhongshan Yang Jie Zhang Chih-Hao Pai Yuqiu Gu Wei Lu | 2020 | Matter and Radiation at Extremes2020，5，6： | 1 |
| 4 | Conducting-polymer microcontainers , Controlled syntheses and potential applications 显示文摘 | Baj pai V He p Dai L | 2004 | Adv Funct Mater2004，14，2： | 1 |
| 5 | Wavelength-tunable blue photoluminescenee of <2 nm Si nanocrystal synthesized by ultra-low-flow-density PECVD显示文摘 | CHANG C H PAI Y H HE J H | 2010 | Aeta Materialia2010，58，4： | 1 |
| 6 | Surgical treatment of tuberculous cold abscess of the chest wall显示文摘 | Pai He Chuny Ky Kang JH | 2002 | Yonse Med2002，43，3： | 1 |
| 7 | Real-world effectiveness of an intranasal spray A8G6 antibody cocktail in the post-exposure prophylaxis of COVID-19显示文摘Previously,we identified an antibody combination A8G6 that showed promising efficacy in COVID-19 animal models and favorable safety profile in preclinical models as well as in a first-in-human trial.To evaluate the real-word efficacy of A8G6 neutralizing antibody nasal spray in post-exposure prophylaxis of COVID-19,an open-label,non-randomized,two-arm,blank-controlled,investigator-initiated trial was conducted in Chongqing,China(the register number:ChiCTR2200066416).High-risk healthy participants(18–65 years)within 72 h after close contact to COVID-19 patients were recruited and received a three-dose(1.4 mg/dose)A8G6 treatment daily or no treatment(blank control)for 7 consecutive days.SARS-CoV-2 infection occurred in 151/340(44.4%)subjects in the blank control group and 12/173(6.9%)subjects in the A8G6 treatment group.The prevention efficacy of the A8G6 treatment within 72 h exposure was calculated to be 84.4%(95%CI:74.4–90.4%).Moreover,compared to the blank-control group,the time from the SARS-CoV-2 negative to the positive COVID-19 conversion was significantly longer in the AG86 treatment group(mean time:3.4 days vs 2.6 days,p=0.019).In the secondary end-point analysis,the A8G6 nasal treatment had no effects on the viral load at baseline SARS-CoV-2 RT-PCR positivity and the time of the negative COVID-19 conversion.Finally,except for 5 participants(3.1%)with general adverse effects,we did not observe any severe adverse effects related to the A8G6 treatment.In this study,the intranasal spray AG86 antibody cocktail showed potent efficacy for prevention of SARS-CoV-2 infection in close contacts of COVID-19 patients. | Xiaosong Li Pai Peng Haijun Deng Qian Yang Shi Chen Benhua Li Miao He Aishun Jin Zhu Yang Ni Tang Ailong Huang | 2023 | Signal Transduction and Targeted Therapy2023，8，11： | 0 |
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