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| 1 | Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion显示文摘AIM: Nitrative and oxidative DNA damage such as 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine(8-oxodG) formation has been implicated in initiation and/or promotion of inflammation-mediated carcinogenesis.The aim of this study is to clarify whether these DNA lesions participate in the progression of intrahepatic cholangiocarcinoma.METHODS: We investigated the relation of the formation of 8-nitroguanine and 8-oxodG and the expression of hypoxia-inducible factor-1α (HIF-1α) with tumor invasion in 37 patients with intra-hepatic cholangiocarcinoma.RESULTS: Immunohistochemical analyses revealed that 8-nitroguanine and 8-oxodG formation occurred to a much greater extent in cancerous tissues than in non-cancerous tissues. HIF-1α could be detected in cancerous tissues in all patients, suggesting low oxygen tension in the tumors.HIF-1α expression was correlated with inducible nitric oxide synthase (iNOS) expression (r= 0.369 and P = 0.025)and 8-oxodG formation (r = 0.398 and P = 0.015).Double immunofluorescence study revealed that iNOS and HIF-1α co-localized in cancerous tissues. Notably, the formation of 8-oxodG was correlated significantly with lymphatic invasion (r = 0.386 and P= 0.018). Moreover, 8-nitroguanine and 8-oxodG in non-cancerous tissues were associated significantly with neural invasion (P = 0.042and P = 0.026, respectively). These results suggest that reciprocal activation between HIF-1α and iNOS mediates persistent DNA damage, which induces tumor invasiveness via mutations, resulting in poor prognosis.CONCLUSION: The formation of 8-nitroguanine and 8-oxodG plays an important role in multiple steps of genetic changes leading to tumor progression, including invasiveness. | Somchai Pinlaor Banchob Sripa Ning Ma Yusuke Hiraku Puangrat Yongvanit Sopit Wongkham Chawalit Pairojkul Vajarabhongsa Bhudhisawasdi Shinji Oikawa Mariko Murata Reiji Semba Shosuke Kawanishi | 2005 | World Journal of Gastroenterology2005，11，30： | 9 |
| 2 | Annexin A1: A new immunohistological marker of cholangiocarcinoma显示文摘AIM: To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC). METHODS: Expression of ANXA1 protein was investigated in liver tissues from patients with CCA and HCC by immunohistochemistry. Its expression on differences stages of tumor development was investigated in hamster CCA tissues induced by Opisthorchis viverrini and N -nitrosodimethylamine. Moreover, mRNA expression of ANXA1 was assessed in CCA cell lines by quantitative real-time polymerase chain reaction and silencing of ANXA1 gene expression using small interfering RNA. RESULTS: In human CCA tissue arrays, immunohistochemical analysis revealed that the positive expression of ANXA1 was 94.1% (64/68 cases) consisting of a high expression (66.2%, 45/68 cases) and a low expression (33.8%, 23/68 cases). However, expression of ANXA1 protein was negative in all histologic patterns for HCC (46/46 cases) and healthy individuals (6/6 cases). In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Grading scores of ANXA1 expression were significantly increased with tumor progression. In addition, mRNA expression of ANXA1 significantly increased in all of the various CCA cell lines tested compared to an immortalized human cholangiocyte cell line (MMNK1). Suppressing the ANXA1 gene significantly reduced the matrix metalloproteinase (MMP) 2 and MMP9, and transforming growth factor-β genes, but increased nuclear factor-kB gene expression. CONCLUSION: ANXA1 is highly expressed in CCA, but low in HCC, suggesting it may serve as a new immunohistochemical marker of CCA. ANXA1 may play a role in opisthorchiasis-associated cholangiocarcinogenesis. | Nuttanan Hongsrichan Rucksak Rucksaken Yaovalux Chamgramol Porntip Pinlaor Anchalee Techasen Puangrat Yongvanit Narong Khuntikeo Chawalit Pairojkul Somchai Pinlaorr | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 3 | Amplification of chromosome 21q22.3 harboring trefoil factor family genes in liver fluke related cholangiocarcinoma is associated with poor prognosis显示文摘瞄准:在 cholangiocarcinoma (CCA ) 包括翘摇因素家庭基因(TFF ) 在染色体区域 21q22-qter 上决定突变而产生之遗传的不平衡病人和分析在突变而产生之遗传的不平衡和 clinicopathological 参数之间的关联。方法:量的 PCR 扩大用一条标准曲线和格林·西布尔在四个微卫星标记和翘摇因素家庭基因(TFF1, TFF2,和 TFF3 ) 上被执行我染料方法。相对拷贝数字被测试地点的 DNA 拷贝数字决定引用地点。当由有正常参考的比较的删除或扩大变化,相对拷贝数字被解释。在突变而产生之遗传的不平衡和 CCA 病人的 clinicopathological 参数之间的协会被 chi (2 ) 评估测试。Kaplan-Meier 方法被用来分析幸存。结果:在 D21S1890, D21S1893,和 TFF3 的扩大的频率分别地是 32.5% , 30.0% ,和 28.7% 。在盖住 D21S1893, D21S1890,和 TFF 的区域有扩大的病人显示出差的预后,而有删除的病人显示出有利预后(平均数:51.7 wk 对 124.82 wk, P = 0.012 ) 。穆尔蒂瓦里伊特·考克斯回归分析表明 D21S1893, D21S1890 和 TFF,血管侵略,和阶段的那扩大与差的预后被联系。结论:D21S1893-D21S1890 区域可以特别怀有候选人基因 TFF 和丝氨酸朊酶家庭,它可能涉及肿瘤侵略和转移贡献穷人幸存。在这个区域的扩大可以在 CCA 病人的治疗被用作一个预示的标记。 | Kanuengnuch Muenphon Temduang Limpaiboon Patcharee Jearanaikoon Chawalit Pairojkul Banchob Sripa Vajarabhongsa Bhudhisawasdi | 2006 | World Journal of Gastroenterology2006，12，26： | 3 |
| 4 | Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma显示文摘AIM: To evaluate the role of thymidine phosphorylase (TP) in cholangiocarcinoma using small interfering RNA (siRNA). METHODS: A human cholangiocarcinoma-derived cell line KKU-M139, which has a naturally high level of endogenous TP, had TP expression transiently knocked down using siRNA. Cell growth, migration, in vitro angiogenesis, apoptosis, and cytotoxicity were assayed in TP knockdown and wild-type cell lines. RESULTS: TP mRNA and protein expression were decreased by 87.1% ± 0.49% and 72.5% ± 3.2%, respectively, compared with control cells. Inhibition of TP significantly decreased migration of KKU-M139, and suppressed migration and tube formation of human umbilical vein endothelial cells. siRNA also reduced the ability of TP to resist hypoxia-induced apoptosis, while suppression of TP reduced the sensitivity of KKU-M139 to 5-fluorouracil. CONCLUSION: Inhibition of TP may be beneficial in decreasing angiogenesis-dependent growth and migration of cholangiocarcinoma but may diminish the response to 5-fluorouracil chemotherapy. | Jongkonnee Thanasai Temduang Limpaiboon Patcharee Jearanaikoon Banchob Sripa Chawalit Pairojkul Srisurang Tantimavanich Masanao Miwa | 2010 | World Journal of Gastroenterology2010，16，13： | 2 |
| 5 | Up-regulation of annexin A2 in cholangiocarcinoma caused by Opisthorchis viverrini and its implication as a prognostic marker显示文摘 | Yonglitthipagon P Pairojkul C Chamgramol Y | 2010 | Int J Parasitol2010，40，10： | 1 |
| 6 | Prognostic significance of peroxiredoxin 1and ezrin-radixin-moesinbinding phosphoprotein 50in cholangiocarcinoma显示文摘 | Yonglitthipagon P Pairojkul C Chamgramol Y | 2012 | Hum Pathol2012，43，10： | 1 |
| 7 | Exome sequencing of liver fluke-associated cholangiocarcinoma显示文摘 | Ong CK Subimerb C Pairojkul C | 2012 | Nat Genet2012，44，6： | 1 |
| 8 | Cholangiocarcinoma: lessons from Thailand显示文摘 | Banchob Sripa Chawalit Pairojkul | 2008 | Current Opinion in Gastroenterology2008，，3： | 1 |
| 9 | Intrahepatic cholangiocarcinoma in Thailand显示文摘 | Thongueb Uttaravichien Vajarabhongsa Bhudhisawasdi Chawalit Pairojkul Ake Pugkhem | 1999 | Journal of Hepato - Biliary - Pancreatic Surgery1999，，2： | 1 |
| 10 | ERBB-2 overexpression and cyclooxygenase-2 up-regulation in human cholangiocarcinoma and risk conditions显示文摘 | Endo K Yoon BI Pairojkul C | | 0，，： | 1 |
| 11 | Mutations of the p53 tumor suppressor gene and the ras gene family in intrahepatic cholangiocellular carcinomas in Japan and Thailand 显示文摘 | Kiba T Tsuda H Pairojkul C | 1993 | Mol Careinog1993，8，4： | 1 |
| 12 | Risk factors and histological outcome of abnormal cervix with human papilloma infection in northeastern Thai-women 显示文摘 | Kanjanavirojkul N Pairojkul C Yuenyao P | 2006 | Asian Pac J Cancer Prey2006，7，4： | 1 |
| 13 | Intrahepatic cholangiocarcinoma in Thailand显示文摘 | Thongueb Uttaravichien Vajarabhongsa Bhudhisawasdi Chawalit Pairojkul Ake Pugkhem | 1999 | Journal of Hepato - Biliary - Pancreatic Surgery1999，，2： | 1 |
| 14 | Prognostic significance of peroxiredoxin 1 and ezrin-radixin-moesin-binding phosphoprotein 50 in cholangiocarcinoma显示文摘 | Yonglitthipagon P Pairojkul C Charngramol Y | 2012 | Hum Pathol2012，43，10： | 1 |
| 15 | Exome sequencing of liver fluke-associated cholangiocarcinoma显示文摘 | Ong CK Subimerb C Pairojkul C | 2012 | Nat Genet2012，44，6： | 1 |
| 16 | Exome sequencing of liver fluke-associated eholangioeareinoma 显示文摘 | Ong CK Subimerb C Pairojkul C | 2012 | Nat Genet2012，44，6： | 1 |
| 17 | ERBB- 2 overexpression and cyclooxygenase-2 up-regulation in human cholangiocarcinoma and risk conditions显示文摘 | Endo K Yoon BI Pairojkul C | 2002 | Hepatology2002，36，2： | 1 |
| 18 | Genetic and epi- genetic alterations of RIZ1 and the correlation to elinicopathologieal parameters in liver fluke-related cholangiocarcinoma显示文摘 | Khaenam P Jearanaikoon P Pairojkul C | 2010 | Exp Ther Med2010，1，2： | 1 |
| 19 | Inflammation-related DNA damage and expression of CD133 and Oct3/4 in cholangiocarcinoma patients with poor prognosis 显示文摘 | Thanan R Pairojkul C Pinlaor S | 2013 | Free Radic Biol Med2013，65，： | 1 |
| 20 | ERBB-2 overexpression and cyclooxygenase-2 up-regulation in human cholangiocarcinoma and risk conditions 显示文摘 | Endo K Yoon B I Pairojkul C | 2002 | Hepatology2002，，： | 1 |
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