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| 1 | Sustained high level transgene expression in mammalian cells mediated by the optimized piggyBac transposon system显示文摘Sustained,high level transgene expression in mammalian cells is desired in many cases for studying gene functions.Traditionally,stable transgene expression has been accomplished by using retroviral or lentiviral vectors.However,such viral vector-mediated transgene expression is often at low levels and can be reduced over time due to low copy numbers and/or chromatin remodeling repression.The piggyBac transposon has emerged as a promising nonviral vector system for efficient gene transfer into mammalian cells.Despite its inherent advantages over lentiviral and retroviral systems,piggyBac system has not been widely used,at least in part due to their limited manipulation flexibilities.Here,we seek to optimize piggyBac-mediated transgene expression and generate a more efficient,user-friendly piggyBac system.By engineering a panel of versatile piggyBac vectors and constructing recombinant adenoviruses expressing piggyBac transposase(PBase),we demonstrate that adenovirusmediated PBase expression significantly enhances the integration efficiency and expression level of transgenes in mesenchymal stem cells and osteosarcoma cells,compared to that obtained from co-transfection of the CMV-PBase plasmid.We further determine the drug selection timeline to achieve optimal stable transgene expression.Moreover,we demonstrate that the transgene copy number of piggyBac-mediated integration is approximately 10 times higher than that mediated by retroviral vectors.Using the engineered tandem expression vector,we show that three transgenes can be simultaneously expressed in a single vector with high efficiency.Thus,these results strongly suggest that the optimized piggyBac system is a valuable tool for making stable cell lines with sustained,high transgene expression. | Xiang Chen Jing Cui Zhengjian Yan Hongmei Zhang Xian Chen Ning Wang Palak Shah Fang Deng Chen Zhao Nisha Geng Melissa Li Sahitya K.Denduluri Rex C.Haydon Hue H.Luu Russell R.Reid Tong-Chuan He | 2015 | Genes & Diseases2015，2，1： | 5 |
| 2 | Intraductal papillary mucinous neoplasm in chronic calcifying pancreatitis:Egg or hen?显示文摘Intraductal papillary mucinous neoplasm(IPMN)is an increasingly reported entity.Extensive pancreatic calcification is generally thought to be a sign of chronic pancreatitis,but it may occur simultaneously with IPMN leading to diagnostic difficulties.We report a case of a patient initially diagnosed with chronic calcifying pancreatitis who was later shown to have a malignant IPMN.This case illustrates potential pitfalls in the diagnosis of IPMN in the case of extensive pancreatic calcification as well as clues that may lead the clinician to suspecting the diagnosis.The possible mechanisms of the relation between pancreatic calcification and IPMN are also reviewed. | Evangelos Kalaitzakis Barbara Braden Palak Trivedi Yalda Sharifi Roger Chapman | 2009 | World Journal of Gastroenterology2009，15，10： | 3 |
| 3 | Biloma: An unusual complication in a patient with pancreatic cancer显示文摘The term biloma describes an encapsulated collection of bile within the abdomen, usually secondary to bile duct disruption. The commonest causes reported in the literature are iatrogenic (secondary to hepatobiliary surgery), trauma or complications due to choledocholithiasis. A few cases have been reported as complications of cholangiocarcinoma or acute cholecystitis. We report the case of a 64-year-old man initially diagnosed with a non-obstructive malignancy of the pancreas, who developed a spontaneous intrahepatic biloma 8 mo later. This was identifi ed following a 1-wk history of fever, rigors and icterus. The biloma was identifi ed on computed tomography and subsequently drained under ultrasound guidance. Forty-eight hours later, a stent was inserted endoscopically into his common bile duct and he made an uneventful in-hospital recovery. We believe this is the fi rst documented case of spontaneous intrahepatic biloma to occur secondary to pancreatic malignancy. | Palak Jitendrakumar Trivedi Prakash Gupta Jane Phillips-Hughes Anthony Ellis | 2009 | World Journal of Gastroenterology2009，15，41： | 3 |
| 4 | Indigenous plant medicines for health care:treatment of Diabetes mellitus and hyperlipidemia显示文摘Medicinal plants have played an important role in treating and preventing a variety of diseases throughout the world. Metabolic syndrome had become a global epidemic, defined as a cluster of three of five criteria: insulin resistance and glucose intolerance, abdominal obesity, hypertension, low high-density cholesterol, and hypertriglyceridemia. The current review focuses on Indian medicinal plant drugs and plants used in the treatment of diabetes and hyperlipidemia. Though there are various approaches to reduce the ill-effects of diabetes and hyperlipidemia and its secondary complications, plant-based drugs are preferred due to lesser side effects and low cost. The current review focuses on twenty-three medicinal plants used in the treatment of Diabetes mellitus and nine medicinal plants used in the treatment of hyperlipidemia. The wealth of knowledge on medicinal plants points to a great potential for research and the discovery of new drugs to fight diseases, including diabetes and hyperlipidemia. | Nisha H. Parikh Palak K. Parikh Charmy Kothari | 2014 | Chinese Journal of Natural Medicines2014，12，5： | 2 |
| 5 | Dentinogenic ghost cell tumor: A case report显示文摘BACKGROUND Dentinogenic ghost cell tumor(DGCT) is an uncommon locally invasive odontogenic neoplasm. It is considered to be a solid variant of calcifying odontogenic cyst(COC). This tumor makes up for only 2%-14% of all COCs and less than 0.5% of all odontogenic tumors which owes to its rarity. The purpose of this paper was to describe a case of DGCT and the treatment adopted in our case,and to provide a review of this case in the indexed literature.CASE SUMMARY In this article, we discussed a case of 18 year old male who reported with a chief complaint of a recurrent swelling and dull aching pain in upper left back region of the jaw. Computed tomography scan was carried out which revealed hypodense lesion with a few hyperdense flecks within it suggesting the presence of calcification. On incisional biopsy, diagnosis of COC was given. After segmental resection of the lesion, histopathogically odontogenic epithelium was noted along with calcifications, ghost cells and dentinoid material. Special staining was done with van Gieson and it showed pink areas of dentinoid material and yellow colour represented ghost cells. Hence, amalgamation of careful clinical examination, use of advanced radiographic imaging and detailed histopathological examination confirmed the diagnosis of DGCT. The patient was followed up for one year and there was no recurrence of the lesion or signs of any residual tumor.CONCLUSION Radical treatment should be carried out along with mandatory long-term follow up in order to avoid recurrence in aggressive lesions. | Sangeeta R Patankar Palak Khetan Sheetal K Choudhari Harshal Suryavanshi | 2019 | World Journal of Clinical Oncology2019，10，4： | 2 |
| 6 | Relationship between incident stroke and severity of internal carotid stenosis 显示文摘 | Palak J Shemanski L O'Leary DH | 1994 | Circulation1994，90，： | 1 |
| 7 | SeverepigmentedkeratitiscausedbyCladorrhinumbulbillosum显示文摘 | GajjarDU PalAK SantosJM etal | 2011 | IndianJMedMicrobiol2011，29，4： | 1 |
| 8 | Detec-ting gene relations from midline abstracts显示文摘 | Stephens M Palakal M Mukhopadhyay S | 2001 | Pacific Symposium on Biocomputing2001，，6： | 1 |
| 9 | Electric current enhanced defect mobility in Ni3 Ti intermetallics 显示文摘 | Javier E Garay Stephen C Glade Palak Asoka-kumar | 2004 | Applied Physics Letters2004，85，4： | 1 |
| 10 | Relationship between incident stroke and the severity of internal carotid stenosis显示文摘 | Palak J Shemanski L Oleary DH | 1994 | Circulation1994，90，： | 1 |
| 11 | A multilevel approach to intelligent information filtering:model,system,and evaluation显示文摘 | MOSTAFA J LAM S W PALAKAL M | 1997 | ACM Transactions on Information Systems1997，15，4： | 1 |
| 12 | Use of computational intelligence for corrosion damage assessment of aircraft panels,uner review,aerosense显示文摘 | Palakal M J Pidaparti R M Jeffrey Huang Rebbapragada S | 2001 | SPIE2001，3637，： | 1 |
| 13 | Relationship be- tween incident stroke and the severity of internal carotid stenosis 显示文摘 | Palak J Shemanski L Oleayy DH | 1994 | Circulation1994，90，1： | 1 |
| 14 | Expression of the survivin gene in the scar endometriosis and in normal human endometrium显示文摘 | Tarkowski R Kotarski J Palak G | 2001 | Ginekol Pol2001，72，12： | 1 |
| 15 | Relationship between incident stroke and the severity of internal carotid stenosis显示文摘 | Palak J Shemanskil L O'Leary DH | 1994 | Circulation1994，90，： | 1 |
| 16 | Relationship between incident stroke and the severity of internal catotid stenosis显示文摘 | Palak J Shemanski L Oleayy DH | 1994 | Circulation1994，90，： | 1 |
| 17 | Relationship between incident stroke and severity of internal carotid stenosis显示文摘 | Palak J Shemansk L O Leary DH | 1994 | Circlation1994，90，11： | 1 |
| 18 | MRI of the small and large bowel显示文摘 | Debalin JF Palak MA | 1999 | Eur Radiol1999，9，8： | 1 |
| 19 | Rough set and intelligent data analysis显示文摘 | Palak Z | 2002 | Information Sciences2002，147，： | 1 |
| 20 | Treating inflammation with the Janus Kinase inhibitor CP-690,550显示文摘 | Lalitha Vijayakrishnan R. Venkataramanan Palak Gulati | 2010 | Trends in Pharmacological Sciences2010，，1： | 1 |
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