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| 1 | Expression of von Hippel-Lindau tumor suppressor and tumor-associated carbonic anhydrases IX and XII in normal and neoplastic colorectal mucosa显示文摘AIM: To analyze possible relationships between CA ⅠⅩ/CA Ⅻ and pVHL expression in normal and neoplastic colorectal mucosa.METHODS: Immunohistochemical staining of 42 tissue specimens obtained from 17 cancer patients was performed to evaluate the distribution and semi-quantitatively assess the levels of CA ⅠⅩ, CA Ⅻ and pVHL. VHL mRNAs from 14 fresh-frozen tumors was amplified by RT-PCR and subjected to sequencing. CA9 and CA12 mRNA levels were analyzed by semi-quantitative RT-PCR in comparison with VEGFas an indicator of hypoxia that uncouples the pVHL control.RESULTS: Tumor tissues were associated with a borderline increase of CA ⅠⅩ staining signal and slight but significant decrease of CA Ⅻ immunoreactivity, whereas no association was found for pVHL. Sequence analysis of RT-PCR-amplified VHL mRNAs revealed no deletions/mutations, suggesting that they were VHL-competent. We did not observe any correlation between pVHL and CA ⅠⅩ/CA Ⅻ proteins as well as between VEGF and CA9 mRNAs, but the tumor-associated changes in mRNA levels of VEGF and CA12 showed a significant inverse relationship.CONCLUSION: Our results indicate that CA9 and CA12 are regulated by different intratumoral factors and that lack of apparent relationship between the levels of CA ⅠⅩ/CA Ⅻ and pVHL cannot be fully assigned to uncoupling of negative regulatory function of pVHL by tumor hypoxia signified by induced VEGF transcription. The interplay between the functional pVHL and CA ⅠⅩ/CA Ⅻ in colorectal tumors seems rather complex and is not evident merely at the expression levels. | Antti J. Kivela Seppo Parkkila Juha Saarnio Tuomo J. Karttunen Jyrki Kivela Anna-Kaisa Parkkila Maria Bartosova Vojtech Mucha Michal Novak Abdul Waheed William S. Sly Hannu Rajaniemi Silvia Pastorekova Jaromir Pastorek | 2005 | World Journal of Gastroenterology2005，11，17： | 10 |
| 2 | Carbonic anhydrase enzymes Ⅱ, Ⅶ, Ⅸ and Ⅻ in colorectal carcinomas显示文摘AIM To investigate expression of four alpha-carbonic anhydrases(CAs) in colorectal carcinomas(CRC) and compare the results with patients' survival.METHODS Colorectal carcinoma samples from 539 CRC patients and control tissues were arranged as tissue microarrays and analyzed with antibodies against CA Ⅱ, CA Ⅶ, CA Ⅸ, and CA Ⅻ. Intensity and extent of staining were both scored from 0 to 3 in each sample. These enzyme expression levels were then correlated to patients' survival and clinicopathological parameters, which were tumor differentiation grade and stage, site of tumor, patients' age, and gender. Kaplan-Meier analysis and Cox regression hazard ratio model were used to analyze survival data. RESULTS CA Ⅱ and CA Ⅻ staining intensities correlated with patients' survival in that higher expression indicated poorer prognosis. In Cox regression analysis one unit increase in the CA Ⅱ intensity increased the hazard ratio to 1.19 fold(CI: 1.04-1.37, P = 0.009). A significant correlation was also found when comparing CA Ⅻ staining intensity with survival of CRC patients(HR = 1.18, 95%CI: 1.01-1.38, P = 0.036). The extent of CA Ⅻ immunostaining did not correlate to the patients' survival(P = 0.242, Kaplan-Meier analysis). A significant interaction between age group and extent of the CA Ⅱ staining was found. Increased extent of CA Ⅱ had a significant hazard ratio among patients 65 years and older(1.42, 95%CI: 1.16-1.73, P = 0.0006). No correlations were found between CA Ⅶ(intensity P = 0.566, extent P = 0.495, Kaplan-Meier analysis), or CA Ⅸ(intensity P = 0.879, extent P = 0.315, KaplanMeier analysis) immunostaining results and survival, or the other parameters. CONCLUSION The present findings indicate that CA Ⅱ and CA Ⅻ could be useful in predicting survival in CRC. | Pia Viikil? Antti J Kivel? Harri Mustonen Selja Koskensalo Abdul Waheed William S Sly Jaromir Pastorek Silvia Pastorekova Seppo Parkkila Caj Haglund | 2016 | World Journal of Gastroenterology2016，22，36： | 3 |
| 3 | Left ventricular torsion is equal in mice and humans 显示文摘 | Henson RE Song SK Pastorek JS Ackerman JJH Lorenz CH | 2000 | Am J Physiol Heart Circ Physiol2000，278，4： | 1 |
| 4 | Molecular mechanisms ofcarbonic anhydrasc IX-mediated pH regulation under hypoxia 显示文摘 | Pastorekova S Ratcliffe PJ Pastorek J | 2008 | BJU Int2008，101，4： | 1 |
| 5 | Carbonic anhydrase IX, MN/CA IX: Analysis of stomach complementary DNA sequence and expression in human and rat alimentary tracts显示文摘 | S Pastorekova S Parkkila AK Parkkila R Opavsky V Zelnik J Saarnio J Pastorek | 1997 | Gastroenterology1997，，2： | 1 |
| 6 | Immunohistochemical Study of Colorectal Tumors for Expression of a Novel Transmembrane Carbonic Anhydrase, MN/CA IX, with Potential Value as a Marker of Cell Proliferation显示文摘 | Juha Saarnio Seppo Parkkila Anna-Kaisa Parkkila Kari Haukipuro Silvia Pastoreková Jaromir Pastorek Matti I. Kairaluoma Tuomo J. Karttunen | 1998 | The American Journal of Pathology1998，，1： | 1 |
| 7 | Cloning and charac- terization of MN, a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix-lcp-helix DNA binding segment显示文摘 | Pastorek J Pastorekov6 S Callebaut I | 1994 | Oncogene1994，9，10： | 1 |
| 8 | Deep plane face-lift显示文摘 | Pastorek N Bustillo A | 2005 | Facial Plast Surg Clin North Am2005，13，3： | 1 |
| 9 | Human tumor-associated cell adhesion protein MN/CA IX:identification of M75 epitope and of the region mediating cell adhesion显示文摘 | Zavada J Zavadova Z Pastorek J | 2000 | Br J Cancer2000，82，： | 1 |
| 10 | Left ventricular torsion is equal in mice and humans显示文摘 | Henson RE Song SK Pastorek JS | 2000 | Am J Physiol Heart Circ Physiol2000，278，4： | 1 |
| 11 | Current outcome of surgical closure of secundum atrial septal defect显示文摘 | Pastorek JS Allen HD Davis JT | 1994 | Am J Cardiol1994，74，： | 1 |
| 12 | Cloning and characterization of MN, a human tumor-associated protein with a domain ho- mologous to carbonic anhydrase and a putative helix-loop-helix DNA binding segment显示文摘 | Pastorek J Pastorekova S Callebant I | 1994 | Oncogene1994，9，10： | 1 |
| 13 | Cloning and characterization of MN, a human tumor-associated protein with a domain homologous to carbonic anhydrase and a putative helix-loop-helix DNA binding segment 显示文摘 | Pastorek J Pastorekova S Callebaut I | 1994 | Oncogene1994，9，10： | 1 |
| 14 | Human tumor-associated cell adhension protein MN/CA IX : identification of M75 eptope and of the region mediating cell adhension 显示文摘 | Zavada J Zavadova Z Pastorek J | 2000 | Br J Cancer2000，82，11： | 1 |
| 15 | Left ventricular torsion is equal in mice and humans显示文摘 | HENSON R E SONG S K PASTOREK J S | 2000 | Am J Physiol Heart Circ Physiol2000，278，4： | 1 |
| 16 | Carbonic Anhydrases: Current State of the Art, Therapeutic Applications and Future Prospects显示文摘 | Pastorekova S Parkkila S Pastorek J | 2004 | J Enzyme Inhib Med Chem2004，19，： | 1 |
| 17 | Comparison of MOS capacitor and transistor postirradiation response显示文摘 | McWhorter P J Fleetwood D M Pastorek R A | 1989 | IEEE transactions on nuclear science1989，36，6： | 1 |
| 18 | Current outcomes of surgical closure of secondum atrial septal defect显示文摘 | Allen HD Davis JT | 1994 | Am J Cardial1994，74，： | 1 |
| 19 | Molecular mechanisms of carbonic anhydrase Ⅸ-mediated pH regulalion under hypoxia 显示文摘 | Pastorekova S Ratcliffe P Pastorek J | 2008 | BJU Int2008，4，： | 1 |
| 20 | Human tumor-associated cell ad- hension protein MN/CA IX:identification of M75 eptope and of the re- gion mediating cell adhension 显示文摘 | Zavada J Zavadova Z Pastorek J | 2000 | Br J Cancer2000，82，11： | 1 |
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