|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Seasonal variation for the antidiabetic activity of Loranthus micranthus methanol extract显示文摘Objective:To determine the season in which the Eastern Nigeria mistletoe,Loranthus micranthus,parasitic on Persea americana possesses optimum antidiabetic activity and to determine the seasonal variation in the constituents.Methods:The antidiabetic activities of the aqueous methanol extracts of the leaves of Eastern Nigeria mistletoe,Loranthus micranthus, harvested in two seasons of the year,the onset of rainy season(April) and the peak of rainy season(July) were compared.The tests were carried out on six(6) groups(A-F) of alloxaninduced diabetic rats.Groups A and B received 200 mg/kg and 400 mg/kg of the April sample extracts respectively while groups C and D received same doses of the July sample extracts. Group E and F which were the positive and negative controls received 10 mg/kg of glibenclamide and 2 ml/kg of 3%tween 20 respectively.The blood glucose levels of the animals were monitored hourly with a glucometer for six hours.The phytochemical analysis of the plant extracts were also carried out by standard procedures.Results:The results showed that group A and B exhibited significant(P<0.05) percentage reduction in the fasting blood sugar(FBS) level of the animals(38.9%and 39.2%respectively) with maximum reduction observed at the 5th and 6th hour respectively compared to glibenclamide(71.3%).Group C showed no significant (P>0.05) FBS reduction(15.9%) while group D exhibited highly significant(P<0.01) reduction (47.5%) with the maximum reduction occurring after 6 hours.The phytochemical analysis of the crude methanol extracts revealed the presence of carbohydrates,glycosides,saponins, tannins,flavonoids,steroids,terpenoids,acidic compounds,resins and oils.These were present in different proportions in both seasons.Conclusions:This study shows that there is a seasonal, dose-dependent variation in the chemical composition viz-a-viz the antidiabetic activity of the plant under study.This activity is highest at the peak of the rainy season. | Patience Ogoamaka Osadebe Edwin Ogechukwu Omeje Philip Felix Uzor Ernest Kenechukwu David Damian Chiedozie Obiorah | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，3： | 4 |
| 2 | Antidiabetic principles of Loranthus micranthus Linn.parasitic on Persea americana显示文摘Objective:To explore antidiabetic principles of the Eastern Nigeria mistletoe,Loranlhus micranthus Linn,parasitic on Persea Americana.Methods:The weakly acidic fraction of the aqueous methanol extract of the leaves of Loranlhus micranthus(Linn.) was isolated and tested for its antidiabetic activities.The isolation of the weakly acidic fraction was carried out following established physico-chemical based procedures.Furthermore,alloxan-induced diabetic rats were treated intraperitoneally(ip) with 250 mg/kg and 400 mg/kg of the weakly acidic fraction, glibenclamide 10 mg/kg(positive control) and 2 mg/kg of 3%v/v tween 20(negative control).The sugar levels of the treated and untreated animals were determined by withdrawing the blood at regular intervals and testing them with an automated glucometer.The phytochemical analysis of the acidic fraction was carried out using standard procedures.Chromatographic techniques were employed in the subsequent isolation and purification of the constituents of the weakly acidic fraction.Results:It was shown that the maximum effect of the weakly acidic fraction was obtained at 24 hours after administration and was found to be statistically comparable with that of the positive control.The phytochemical analysis revealed the presence of steroids,terpenoids, alkaloids,flavonoids,glycosides,carbohydrates,saponins,and acidic compounds in the crude extract and carbohydrates,flavonoids.terpenoids and oil in the weakly acidic fraction.Further purification of the weakly acidic fraction of the methanol extract using thin layer chromatography shows that toluene:methanol:diethyl amine(3:1:1) and chloroform:methanol:diethyl amine (9:1:1) are the best solvent system for the isolation of the various components of the weakly acidic fraction of the crude methanol extract of Loranlhus micranthus.Conclusions:The present study has led to the conclusion that the weakly acidic fraction of the plant under study has the potent antidiabetic activity and that the various components can be isolated using basic chromatographic techniques. | Patience Ogoamaka Osadebe Edwin Ogechukwu Omeje Sylvester Chukwuemeka Nworu Charles Okechukwu Esimone Philip Felix Uzor Ernest kenechukwu David John Uchechukwu Uzoma | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，8： | 4 |
| 3 | A Mother-to-Child Transmission Study in Nigeria:The Impact of Maternal HIV Infection and HAART on Plasma Immunoglobulins,Cytokine Profiles and Infant Outcome显示文摘Prevention of mother-to-child transmission(PMTCT)of HIV with highly active antiretroviral therapy(HARRT)allows the HIV^+pregnant mothers to have vaginal delivery and breastfeed.Here we investigated the maternal plasma immunoglobulin,cytokine secretion and the outcome of the exposed infants among the HIV^+HAART treated pregnan women in Nigeria.In this study,different plasma immunoglobulins and cytokines were measured in the HIV^+HAART treated pregnant mothers.Pooled culture supernatants of B and T lymphocytes showed lower levels of IFN-γ,IL-10 and IL-4.There were lower IFN-γand IL-10 secretions at 1st trimester;however,IL-10 continued to be lower throughout 2nd and 3rd trimesters.TNF-αsecretion significantly decreased as pregnancy progressed to term.There were high plasma IgG and low IgM in the HIV^+HAART treated pregnant women.Plasma IgG was high during 1st and 3rd trimesters.After one year of follow up,all the exposed children were seronegative for HIV-1 and HIV-2.Vaginal delivery and breastfeeding among HIV^+HAART treated mothers have shown to be safe.The use of HAART by the infected mothers and the use of septrin and niverapin by the exposed infants prevented mother to-child transmission of HIV. | Chinwe O.Ewenighi-Amankwah Charles Chinedum Onyenekwe Ogochukwu Udemba Patience Muogbo Lijun Rong | 2020 | Virologica Sinica2020，35，4： | 3 |
| 4 | 热应激和低营养摄入对猪肠道完整性和功能的影响显示文摘热应激会损害肠道完整性,并诱发肠渗漏。因此,本试验研究了直接或间接热应激(通过减少采食量)对生长猪肠道通透性的影响。选用体重(43±4)kg的杂交后备母猪(n=48),分别安置在以下控制环境中:热中性(TN)环境(20℃,湿度35%~50%,自由采食)、热应激(HS)环境(35℃,湿度20%~35%,自由采食)、热中性环境配对饲喂(PFTN,以消除不同采食量的交互影响)。第1、3、7天使猪只安乐死,采集的空肠样品被安装到尤式灌流室,测定跨膜电阻抗值(TER)和异硫氰基荧光标记的脂多糖(LPS)渗透性(APP,表示为表观渗透系数)。此外,评估肠道完整性以及应激基因和蛋白标志物。结果表明,不考虑热应激天数,HS组与TN组猪相比,血浆内毒素水平增加45%(P〈0.05),空肠TER降低30%(P〈0.05),LPS渗透率增加2倍(P〈0.01)。此外,与PFTN组相比,HS组第7天的猪LPS渗透性有增加的趋势(P=0.06)。整个试验阶段HS组溶菌酶和碱性磷酸酶活性下降,分别下降46%和59%(P〈0.05);然而,3 d和7 d空肠免疫细胞标记物——髓过氧化物酶活性增加(P〈0.05)。由此可见,热应激和减少采食量都会降低肠道完整性,并增加内毒素渗透性。推测这可能会导致炎症加重,在炎热的夏季可能是导致猪生长性能降低的原因。 | Pearce S C Mani V Weber T E Rhoads R P Patience J F Baumgard L H Gabler N K | 2016 | 中国饲料2016，，3： | 3 |
| 5 | Phytochemical analysis,hepatoprotective and antioxidant activity of Alchornea cordifolia methanol leaf extract on carbon tetrachloride-induced hepatic damage in rats显示文摘Objective:To investigate the hepatoprotective and antioxidant activities of Alchornea cordifolia (A.cordifolia) leaf extract.Methods:Various solvent fractions of the methanol extract of the leaf of the plant.4.cordifolia Mull.Arg(Fam:Euphorbiaceae) were evaluated for hepatoprotective activity by carbon tetrachloride-induced liver damage in rats.The degree of protection was measured by using biochemical parameters such as serum glutamate oxalate transaminase(SGOT/ AST),serum glutamate pyruvate transaminase(SCPT/ALT),alkaline phosphatase(ALP) and total bilirubin.The in vitro antioxidant activity of the extract was also evaluated by the I,I-diphenyl 2-picrylhydrazyl(DPPH) free radical scavenging assay.The extract was subjected to preliminary phytoehemical screening.Results:The ethyl acetate and chloroform fractions,at a dose of 300 mg/kg,produced significant(P<0.05) hepatoprolection by decreasing the activities of the serum enzymes and bilirubin while there were marked scavenging of the DPPH free radicals by the fractions.The effects were comparable to those of the standard drugs used for the respective experiments,silymarin and ascorbic acid.Alkaloids,flavonoids,saponins and tannins were delected in the phylochemical screening.Conclusion:From this study,it was concluded that the plant of A.cordifolia possesses hepatoprotective as well as antioxidant activities and these activities reside mainly in the ethyl acetate and acetone fractions of methanol leaf extract. | Patience O Osadebe Festus BC Okoye Philip F Uzor Nneka R Nnamani Ijeoma E Adiele Nkemakonam C Obiano | 2012 | Asian Pacific Journal of Tropical Medicine2012，5，4： | 2 |
| 6 | 日粮成分对生长猪饮水量的影响显示文摘1 前言
历史上,人们一直把水看成是充裕而廉价的资源,所以缺乏确定猪需水量或对影响水利用情况因素进行研究(Fraser等,1990)的积极性。现在,人类社会正越来越意识到水是有限的资源,而家畜生产行业却又是一个主要的耗水行业之一。对肉猪生产者来说,过度用水会产生过多的厩粪,而厩粪储存和田间施用的费用都很高。因此,肉猪生产者日益有兴趣于既能减少用水量又不危害猪生长性能的管理方法。日粮调整不失为一个可能解决问题的策略。 | M. I. Shaw A. D. Beaulieu J. F. Patience 陈春月(译) 覃矜(校) 樊薛安(摘) | 2007 | 国外畜牧学(猪与禽)2007，，6： | 2 |
| 7 | Heat transfer studies inan inorganic membrane reactor at pilot plant s(-ale显示文摘 | Alonso M Patience G Fernandez J R | 2006 | CatalysisToday2006，118，12： | 1 |
| 8 | Effects of supplemental zinc amino acid complex on gut integrity in heat-stressed growing pigs显示文摘 | M. V. Sanz Fernandez S. C. Pearce N. K. Gabler J. F. Patience M. E. Wilson M. T. Socha J. L. Torrison R. P. Rhoads L. H. Baumgard | 2013 | animal2013，，1： | 1 |
| 9 | Phosphorous modified ZSM-5:deactivation and product distribution for MTO显示文摘 | Kaarsholm M Joensen F Nerlov J Cenni R Chaouki J Patience G S | | 0，，18： | 1 |
| 10 | Infection of human cells by an endogenous retrovirus of pigs显示文摘 | Patience C Takeuchi Y Weiss R A | 1997 | Nat Med1997，3，3： | 1 |
| 11 | Structure and significance of C30 4-methyl stranes in lacustrine shales and oils显示文摘 | Goodwin N S Mann A L Patience R L | 1988 | Organic Geochemistry1988，12，5： | 1 |
| 12 | Molecular parameters of maturation in the Toarcian shales,Paris,France Ⅰ:changes in the configuration of the acyclic isoprenoid alkanes,steranes and triterpanes显示文摘 | Mackenzie A S Patience R L Maxwell J R | 1980 | Geochimica et Cosmochimica Acta1980，44，： | 1 |
| 13 | Infection of human cells by an endogenous retrovirus of pigs显示文摘 | Patience C Takeuchi Y Weiss RA | 1997 | Nat Med1997，3，： | 1 |
| 14 | Effect of dietary electrolyte balance on growth and acid-base status in swine 显示文摘 | Patience J F Austic R E Boyd R D | 1987 | J Animal Science1987，64，2： | 1 |
| 15 | The energy content of barley fed to growing pigs: characterizing the nature of its variability and developing prediction equations for its es- timation显示文摘 | Fairbairn S L Patience J F Classen H L | 1999 | Journal of Animal Science1999，77，6： | 1 |
| 16 | Investigation of the effect of polymer type on flocculation,rheology and dewatering behaviour of kaolinite dispersions显示文摘 | Patience M Addai-Menash J Ra1ston J | 2003 | Int J Miner Process2003，71，： | 1 |
| 17 | Xylanase supplementation improves energy digestibility of wheat by-products in grower pigs显示文摘 | Nortey T N Patience J F Sands J S | 2007 | Livestock Science2007，92，1： | 1 |
| 18 | Membrane pilot reactorapplied to selective oxidation reactions 显示文摘 | Alonso M Lorences M J Patience G S | 2005 | Catalysis Today2005，104，234： | 1 |
| 19 | Gene therapy-its potential in surgery显示文摘 | Gojo S Yamamoto S Patience C | 2002 | Ann R Coil Surg Engl2002，84，5： | 1 |
| 20 | Host range and interference studies of three classes of pig endogenous retrovirus 显示文摘 | Takeuchi Y Patience C Magre S | 1998 | J Virol1998，72，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员