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| 1 | Lymphocyte subsets in alcoholic liver disease显示文摘AIM:To compare lymphocyte subsets between healthy controls and alcoholics with liver disease.METHODS:The patient cohort for this study included individuals who were suspected to have alcoholic liver disease(ALD) and who had undergone liver biopsy(for disease grading and staging,doubts about diagnosis,or concurrent liver disease;n = 56).Normal controls included patients who were admitted for elective cholecystectomy due to non-complicated gallstones(n = 27).Formalin-fixed,paraffin-embedded liver biopsy specimens were sectioned and stained with hematoxylin and eosin and Perls' Prussian blue.The non-alcoholic steatohepatitis score was used to assess markers of ALD.Lymphocyte population subsets were determined by flow cytometry.T lymphocytes were identified(CD3+),and then further subdivided into CD4+ or CD8+ populations.B lymphocytes(CD19+) and natural killer(NK) cell numbers were also measured.In addition to assessing lymphocyte subpopulation differences between ALD patients and controls,we also compared subsets of alcoholic patients without cirrhosis or abstinent cirrhotic patients to normal controls.RESULTS:The patient cohort primarily consisted of older men.Active alcoholism was present in 66.1%.Reported average daily alcohol intake was 164.9 g and the average lifetime cumulative intake was 2211.6 kg.Cirrhosis was present in 39.3% of the patients and 66.1% had significant fibrosis(perisinusoidal and portal/periportal fibrosis,bridging fibrosis,or cirrhosis) in their liver samples.The average Mayo end-stage liver disease score was 7.6.No hereditary hemochromatosis genotypes were found.ALD patients(n = 56) presented with significant lymphopenia(1.5 × 109/L ± 0.5 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001),due to a decrease in all lymphocyte subpopulations,except for NK lymphocytes:CD3+(1013.0 ± 406.2/mm3 vs 1523.0 ± 364.6/mm3,P < 0.0001),CD4+(713.5 ± 284.7/mm3 vs 992.4 ± 274.7/mm3,P < 0.0001),CD8+(262.3 ± 140.4/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(120.6 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).CD8+ lymphocytes suffered the greatest reduction,as evidenced by an increase in the CD4+/CD8+ ratio(3.1 ± 1.3 vs 2.3 ± 0.9,P = 0.013).This ratio was associated with the stage of fibrosis on liver biopsy(rs = 0.342,P = 0.01) and with Child-Pugh score(rs = 0.482,P = 0.02).The number of CD8+ lymphocytes also had a positive association with serum ferritin levels(rs = 0.345,P = 0.009).Considering only patients with active alcoholism but not cirrhosis(n = 27),we found similar reductions in total lymphocyte counts(1.8 × 109/L ± 0.3 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P = 0.018),and in populations of CD3+(1164.7 ± 376.6/mm3 vs 1523.0 ± 364.6/mm3,P = 0.001),CD4+(759.8 ± 265.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.003),CD8+(330.9 ± 156.3/mm3 vs 478.9 ± 164.6/mm3,P = 0.002),and CD19+(108.8 ± 64.2/mm3 vs 264.6 ± 88.0/mm3,P < 0.0001).In these patients,the CD4+/CD8+ ratio and the number of NK lymphocytes was not significantly different,compared to controls.Comparing patients with liver cirrhosis but without active alcohol consumption(n = 11),we also found significant lymphopenia(1.3 × 109/L ± 0.6 × 109/L vs 2.1 × 109/L ± 0.5 × 109/L,P < 0.0001) and decreases in populations of CD3+(945.5 ± 547.4/mm3 vs 1523.0 ± 364.6/mm3,P = 0.003),CD4+(745.2 ± 389.0/mm3 vs 992.4 ± 274.7/mm3,P = 0.032),CD8+(233.9 ± 120.0/mm3 vs 478.9 ± 164.6/mm3,P < 0.0001),and CD19+(150.8 ± 76.1/mm3 vs 264.6 ± 88.0/mm3,P = 0.001).The NK lymphocyte count was not significantly different,but,in this group,there was a significant increase in the CD4+/CD8+ ratio(3.5 ± 1.3 vs 2.3 ± 0.9,P = 0.01).CONCLUSION:All patient subsets presented with decreased lymphocyte counts,but only patients with advanced fibrosis presented with a significant increase in the CD4+/CD8+ ratio. | Luís Costa Matos Paulo Batista Nuno Monteiro Joo Ribeiro Maria A Cipriano Pedro Henriques Fernando Giro Armando Carvalho | 2013 | World Journal of Hepatology2013，5，2： | 4 |
| 2 | Chemical composition of Rosmarinus officinalis essential oil and antioxidant action against gastric damage induced by absolute ethanol in the rat显示文摘Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were prepared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured.Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of(140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis(50 mg/kg, p.o.), a significant inhibition of mucosal injury of(21.2 ± 7.1) mm2(84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes(superoxide dismutase and glutathione peroxidase) and increasing or maintaining the levels of glutathione. In addition,lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography–mass spectrometer and the main constituents were cineole(28.5%),camphor(27.7%) and alpha-pinene(21.3%).Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis. | Christiane Takayama Felipe Meira de-Faria Ana Cristina Alves de Almeida Ricardo Jose Dunder Luis Paulo Manzo Eduardo Augusto Rabelo Socca Leonia Maria Batista Marcos Jose Salvador Alba Regina Monteiro Souza-Brito Anderson Luiz-Ferreira | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，8： | 3 |
| 3 | 体外评估自酸蚀粘结剂的釉质封闭能力显示文摘目的:尽管有报道自酸蚀釉质粘合剂存在粘结不足的缺陷.自酸蚀粘结剂的应用在不断扩大.有些临床医师选择单独酸蚀釉质以弥补其缺陷。本研究目的是评估3种自酸蚀粘结剂在热疲劳后,酸蚀对釉质封闭的影响。材料与方法:取30颗牛切牙,在每颗牙上预备两个箱状洞型,分别用Adper Prompt L-Pop(3MESPE)、Clearfil SE Bond(Kuraray America)和iBond(Heraeus Kulzer)这三种自酸蚀粘结剂中的一种处理窝洞。其中部分先用磷酸酸蚀釉质,另一部分直接涂布自酸蚀粘结剂,然后用FiltekZ250(3MESPE)充填洞型。然后取一半的充填体置于1500个热循环中使之疲劳,再用50%硝酸氨银来检验材料的边缘封闭能力。银离子渗透按O~4分级.材料界面通过黑点探测器在发散区域扫描电镜(FESEM)下分析。用非参数统计计算出一个95%的可信区间。结果:Clearfil SE Bond的釉质微渗漏显著低于另两个自酸蚀粘结剂。对置于热循环后的标本,酸蚀能显著降低所有自酸蚀粘结剂的银离子渗透。对没有经过热循环的标本,酸蚀只能减少iBond的银离子渗透。在FESEM下.银离子沉淀可被发现位于Adper Prompt L-Pop和iBond的粘结层中.而Clearfil SE Bond处理后的10个标本中只有2个表现出银离子沿釉质粘结界面渗透。结论:标本在热循环疲劳状态.磷酸酸蚀能提高3种自酸蚀粘结剂的釉质边缘封闭能力。 | Jorge Perdigǎo Paulo Monteiro George Gomes 李珍(译) | 2009 | 中国口腔医学继续教育杂志2009，，4： | 2 |
| 4 | Animal modeling in bone research—Should we follow the White Rabbit?显示文摘Animal models are live subjects applied to translational research.They provide insights into human diseases and enhance biomedical knowledge.Livestock development has favored the pace of human social development over millennia.Today's society is more aware of animal welfare than past generations.The general public has marked objections to animal research and many species are falling into disuse.The search for an ideal methodology to replace animal use is on,but animal modeling still holds great importance to human health.Bone research,in particular,has unmet requirements that in vitro technologies cannot fully address.Standardizing novel models remains necessary and rabbits are gaining in popularity as potential bone models.Our aim here is to provide a broad overview of animal modeling and its ethical implications,followed by a narrower focus on bone research and the role rabbits are playing in the current scenario. | Aline Schafrum Macedo Caroline Cezaretti Feitosa Fernando Yoiti Kitamura Kawamoto Paulo Vinicius Tertuliano Marinho Isis dos Santos Dal‐Bo Bianca Fiuza Monteiro Leonardo Prado Thales Bregadioli Gabriel Antonio Covino Diamante Cassio Ricardo Auada Ferrigno | 2019 | Animal Models and Experimental Medicine2019，2，3： | 2 |
| 5 | Alkali-silica Reaction,Part 2:Use of the Double-layer Theory to Explain the Behavior of Reaction-product Gels显示文摘 | Paulo J M Monteiro Garrison Sposito | 1997 | ACI Materials Journal1997，，1： | 1 |
| 6 | Alkali-silica Reaction,Part 2:The Effect of Chemical Admixtures显示文摘 | Paulo J M Monteiro Garrison Sposito | 1998 | ACI Materials Journal1998，，1： | 1 |
| 7 | Inhibition of tumor necrosis factor-alpha and cyclooxigenase-2 by Isatin: A molecular mechanism of protection against TNBS-induced colitis in rats显示文摘 | Eduardo Augusto Rabelo Socca Anderson Luiz-Ferreira Felipe Meira de Faria Ana Cristina de Almeida Ricardo José Dunder Luis Paulo Manzo Alba Regina Monteiro Souza Brito | 2014 | Chemico-Biological Interactions2014，，： | 1 |
| 8 | Thestudy of disorder and nanocrystallinity in C-S-H,sup-plementary cementitious materials and geopolymers u-sing pair distribution function analysis显示文摘 | Meral Cagla Benmore C J Monteiro Paulo J M | 2011 | Cementand Concrete Reseach2011，41，： | 1 |
| 9 | Time to Failure for Concrete Exposed to Severe Sulfate Attack显示文摘 | Paulo J M Monteiro Kimberly E Kurtis | 2003 | Cement and Concrete Research2003，33，7： | 1 |
| 10 | Effect of carbon fibre reinforcement in the frictional behaviour of PEEK in a water lubricated environment显示文摘 | J Paulo Davim Nuno Marques A Monteiro Baptista | 2001 | Wear2001，251，： | 1 |
| 11 | X-ray Diffraction Investigations of Microstructure of Calcium Hydroxide Crystallites in the Interfacial Transition Zone of Concrete显示文摘 | Harutyunyan Valeri S Abovyan Eduward S Monteiro Paulo J M | 2003 | Journal of the American Ceramic Society2003，86，12： | 1 |
| 12 | The influence of polymerson the hydration of Portland cement phases analyzed by soft X-ray transmission microscopy 显示文摘 | Denise A S Paulo J M Monteiro | 2006 | Cem Concr Compos2006，34，： | 1 |
| 13 | A model to predict the amount of calcium hydroxide in concrete containing mineral admixtures显示文摘 | Tatsuhiko Saeki Paulo J.M. Monteiro | 2005 | Cement and Concrete Research2005，，： | 1 |
| 14 | The decomposition of excess demand functions on banach spaces显示文摘 | Monteiro Paulo Kinger | 1994 | Journal of Mathematical Economics1994，1，： | 1 |
| 15 | Surface Characterization of Recycled Tire Rubber to Be Used in Cement Paste Matrix显示文摘 | Nadia Segre Paulo J.M. Monteiro Garrison Sposito | 2002 | Journal of Colloid And Interface Science2002，，2： | 1 |
| 16 | Surface characterization of recycled tire rubber to be used in cement paste matrix显示文摘 | Segre Nadia Monteiro Paulo J M Sposito Garrison | 2005 | Journal of Colloid andInterlace Science2005，2482，： | 1 |
| 17 | Surface characterization of recycled tire rubber to be used in cement paste matrix 显示文摘 | Nadia Segre Paulo J M Monteiro Garrison Sposito | 2002 | Colloid and Interface Science2002，，248： | 1 |
| 18 | Kras Mutation Analysis of Fine Needle Aspirate Under EUS Guidance Facilitates Risk Stratification of Patients With Pancreatic Mass显示文摘 | Fauze Maluf-Filho Atul Kumar René Gerhardt Márcia Kubrusly Paulo Sakai Fabio Hondo Sergio Eiji Matuguma Everson Artifon José Eduardo Monteiro da Cunha Marcel Cerqueira César Machado Shinichi Ishioka Elias Forero | 2007 | Journal of Clinical Gastroenterology2007，，10： | 1 |
| 19 | The influence of polymers on the hydration of Portland cement phases analyzed by soft X-ray transmission microscopy 显示文摘 | Denise A S Paulo J M Monteiro | 2006 | Cem Concr Compos2006，34，： | 1 |
| 20 | Noninvasive Surface Measurement of Corrosion Impedance of Reinforcing Bar in Concrete-part1 显示文摘 | Zhang Jieying Monteiro Paulo J M Morrison H Frank | 2001 | ACI Materials Journal2001，98，2： | 1 |
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