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| 1 | Using the polymerase chain reaction coupled with denaturing gradient gel electrophoresis to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted acute severe pancreatitis显示文摘AIM: To investigate the use of PCR and DGGE to investigate the association between bacterial translocation and systemic inflammatory response syndrome in predicted severe AP.METHODS: Patients with biochemical and clinical evidence of acute pancreatitis and an APACHE Ⅱ score ≥8 were enrolled. PCR and DGGE were employed to detect bacterial translocation in blood samples collected on d1,3, and 8 after the admission. Standard microbial blood cultures were taken when there was clinical evidence of sepsis or when felt to be clinically indicated by the supervising team.RESULTS: Six patients were included. Of all the patients investigated, only one developed septic complications;the others had uneventful illness. Bacteria were detected using PCR in 4 of the 17 collected blood samples. The patient with sepsis was PCR-positive in two samples (taken on d 1 and 3), despite three negative blood cultures. Using DGGE and specific primers, the bacteria in all blood specimens which tested positive for the presence of bacterial DNA were identified as E coli.CONCLUSION: Our study confirmed thatunlike traditional microbiological techniques, PCR can detect the presence of bacteria in the blood of patients with severe AP. Therefore, this latter method in conjunction with DGGE is potentially an extremely useful tool in predicting septic morbidity and evaluating patients with the disease. Further research using increased numbers of patients, in particular those patients with necrosis and sepsis, is required to assess the reliability of PCR and DGGE in the rapid diagnosis of infection in AP. | Callum B Pearce Vitaly Zinkevich Iwona Beech Viera Funjika Ana Garcia Ruiz Afraa Aladawi Hamish D Duncan | 2005 | World Journal of Gastroenterology2005，11，45： | 11 |
| 2 | Does cryotherapy before drainage increase the risk of intraocular hemorrhage and affect outcome? 显示文摘 | Pearce I A Wang D Ocata W | 1997 | Br J Ophthalmol1997，81，6： | 2 |
| 3 | Molecular determination of point mutation haplotypes in the dihydrofolate reductase and dihydropteroate synthase of Plasmodium falciparum in three districts of northern Tanzania显示文摘 | Pearce RJ Drakeley C Chandramohan D | 2003 | Antimierob Agents Chemother2003，47，4： | 1 |
| 4 | Spatial patterns of package tourism in Europe显示文摘 | Pearce D G | 1987 | Annals of Tourism Research1987，14，2： | 1 |
| 5 | Tectonic implications of the composition of volcanic arc magmas显示文摘 | PEARCE J A PEATE D W | 1995 | Annual Reviews of Earth and Planetary Science1995，23，： | 1 |
| 6 | Significant changes in the bacterioplankton community structure of a maritime Antarctic fieshwater lake following nutrient enrichment显示文摘 | PEARCE D A van der GAST C J WOODWARD K | 2005 | Microbiology2005，151，10： | 1 |
| 7 | Measuring sustainable development:Progress on indicators 显示文摘 | Pearce D K Hamilton A | 1996 | Environmental and Development Economics1996，，1： | 1 |
| 8 | Ramsdellite-MnO2 for lithium batteries: The ramsdellite to spinel transformation显示文摘 | THACKERAY M M ROSSOUW M H GUMMOW R J LILES D C PEARCE K KOCK A D DAVID W I F HULLS S | 1993 | Electrochim Acta1993，38，9： | 1 |
| 9 | Characterization of programmed cell death during aerenchyma formation induced by ethylene of hypoxia in roots of maize (Zea mays L)显示文摘 | GUNAWARDENA A PEARCE D M JACKSON M B etal | 2001 | Planta2001，212，1： | 1 |
| 10 | Decentralised R&D and strategic competitiveness:globalised approaches to generation and use of technology in multinational enterprises (MNEs) 显示文摘 | Pearce R D | 1999 | Re- search Policy1999，28，23： | 1 |
| 11 | Activation tagging of a dominant gibberellin catabolism gene (GA 2-oxidase) from poplar that regulates tree stature显示文摘 | BUSOV V B MEILAN R PEARCE D W | 2003 | Plant Physiol2003，132，3： | 1 |
| 12 | The political economy of an energy tax: the United Kingdom 's climate change levy 显示文摘 | Pearce D | 2006 | Energy Economics2006，28，2： | 1 |
| 13 | Nature of remanence carried by Allende chondrules显示文摘 | Lanoix M Strangway D W Pearce G W | 1978 | Transactions-American Geophysical Union1978，，59： | 1 |
| 14 | Investigat-ion of cyclic thermal shock behavior of fiber reinforced glass matrix composites using non-destructive forced resonance technique显示文摘 | Boccaccini A R Pearce D H | 1997 | Mater Sci Techn1997，13，10： | 1 |
| 15 | Melting Temperature of High-Temperature Fixed Points for Thermocouple Calibrations显示文摘 | Pearce J V Montag V Lowe D | 2011 | International Journal of Thermophysics2011，32，1： | 1 |
| 16 | The proinflammatory cytokine,interleukin-1alpha,reduces glucocorticoid receptoR translocation and function显示文摘 | Pariante C M Pearce B D Pisell T L | 1999 | Endocrinology1999，140，9： | 1 |
| 17 | Hematopoietic stem ceils express multiple myeloid markers: implications for the origin and targeted therapy of acute myeloid leukemia显示文摘 | Taussig DC Pearce D J Simpson C | 2005 | Blood2005，106，13： | 1 |
| 18 | Optimal information transmission in nonlinear arrays through suprathreshold stochastic resonance 显示文摘 | M D McDonnell N G Stocks C E M Pearce D Abbott | 2006 | Physics Letters A2006，352，3： | 1 |
| 19 | A polypeptide from tomato leaves activates the expression of proteinase inhibitor genes显示文摘 | PEARCE G STRYDOM D JOHNSON S | 1991 | Science1991，253，： | 1 |
| 20 | Production of multiple plant hormones from a single poly protein precursor显示文摘 | Pearce G Moura D S Stratmann J | 2001 | Nature2001，411，6839： | 1 |
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