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| 1 | 急性肾小球肾炎的循证诊治指南显示文摘该指南由中国人民解放军医学会儿科分会肾脏病学组组织起草,以当前儿童原发性急性肾小球肾炎循证诊治的最佳临床实践证据,为儿科医师提供符合中国国情、可操作的儿童原发性急性肾小球肾炎诊断和治疗指南。 | Pediatrics Nephrology Group of The People’s Liberation Army Medical Association | 2013 | 临床儿科杂志2013，31，6： | 28 |
| 2 | Expression and signifi cance of TLR4 and HIF-1α in pancreatic ductal adenocarcinoma显示文摘AIM:To investigate the expression of toll-like receptor(TLR) 4,nuclear factor-κB(NF-κB) p65 and hypoxiainducible transcription factor 1α(HIF-1α) in pancreatic ductal adenocarcinoma and their clinical significance.METHODS:The mRNA of TLR4 and HIF-1α were investigated by real-time polymerase chain reaction in 30 cases of pancreatic ductal adenocarcinoma and its adjacent tissues,and expression of TLR4,NF-κB p65 and HIF-1α protein were detected by immunohistochemistry in 65 cases of pancreatic ductal adenocarcinoma tissues and 38 cases of corresponding adjacent tissues.The relationship between TLR4 or HIF-1α and pathologic features,as well as the association between TLR4 and HIF-1α,were also analyzed.Kaplan-Meier method was used to assess the impact of expression of TLR4 and HIF-1α on survival of patients with pancreatic cancer.RESULTS:The relative quantif ication of TLR4 and HIF-1α mRNA in tumor tissues was 0.81±0.10 and 0.87±0.11,respectively,signif icantly higher than that in adjacent tissues(0.81±0.10 vs 0.70±0.16,P=0.002;0.87±0.11 vs 0.68±0.13,P=0.000).The protein expression of TLR4,NF-κB p65 and HIF-1α in tumor tissues was 69.20%,66.15% and 70.80%,respectively,being signif icantly higher than that in adjacent normal tissues(69.20% vs 39.50%,P=0.003;66.15% vs 31.58%,P=0.001;70.80% vs 36.80%,P=0.001).There was no signif icant correlation between TLR4 or HIF-1α expression and the age,gender,tumor location,the degree of tumor differentiation in the patients(P>0.05).However,there was signif icant correlation between the expression of TLR4 or HIF-1α and tumor size,lymph node metastasis,venous invasion and clinical staging(P<0.05).The expression of TLR4 and HIF-1α had a signif icant impact on survival of patients with pancreatic adenocarcinoma.CONCLUSION:TLR4,NF-κB p65 and HIF-1α are overexpressed in pancreatic adenocarcinoma,TLR4 may be partly involved in up-regulating HIF-1α,and both synergestically promote development of pancreatic adenocarcinoma. | Jian-Jun Zhang,He-Shui Wu,Lin Wang,Yuan Tian,Jing-Hui Zhang,Hai-Long Wu Department of Pancreatic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Department of Pediatrics,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China Laboratory of General Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,Hubei Province,China | 2010 | World Journal of Gastroenterology2010，16，23： | 21 |
| 3 | 中国新生儿营养支持临床应用指南显示文摘 | (The group of Pediatrics, Chinese Parenteral and Enteral Nutrition Society, Chinese Medical Association,the group of Neonatology, Chinese Pediatric Society, Chinese Medical Association,the group of Neonatology, Chinese Pediatric Surgical Society, Chinese Medical Association) | 2006 | 肠外与肠内营养2006，13，6： | 18 |
| 4 | Expression of Pdx-1 in bone marrow mesenchymal stem cells promotes differentiation of islet-like cells in vitro显示文摘Bone marrow mesenchymal stem cells(BMSCs) have the ability of self-renewal and multi-directional differentiation.Recent reports showed that BMSCs could differentiate into endocrine cells of pancreas.However,the differentiation is not efficient enough to produce insulin-producing cells for the future therapeutic use.Pdx-1 is a crucial regulator for pancreatic development.Therefore we constructed a eukaryotic expression vector containing Pdx-1 to determine the effect of Pdx-1 ex-pression on differentiation of BMSCs in vitro.The results showed that BMSCs could self-assemble to form functional pancreatic islet-like structures after differentiation in vitro.The proportion of insu-lin-producing cells differentiated from Pdx-1+BMSCs was 28.23%±2.56%,higher than that from BMSCs transfected with vacant vector and Pdx-1-BMSCs(7.23%±1.56% and 4.08%±2.69% respec-tively) by flow cytometry.Immunocytochemical examination also testified the expression of multiple β-cells-specific genes such as insulin,glucagons,somatostatin in differentiated BMSCs.The results also revealed that the expressions of genes mentioned above in Pdx-1+BMSCs were higher than that in Pdx-1-BMSCs,which was confirmed by Western blotting analysis and RT-PCR.Glucose-induced insulin secretion from Pdx-1+BMSCs in 5mmol/L and 25mmol/L glocuse was(56.61±4.82) μU/mL and(115.29±2.56) μU/mL respectively,which were much higher than those from Pdx-1-BMSCs((25.53±6.49) μU/mL and(53.26±7.56) μU/mL respectively) .Grafted animals were able to maintain their body weight and survive for relatively longer periods of time than hyperglycemic sham-grafted controls,which demonstrated an overall beneficial effect of the grafted cells on the health of the animals.These findings thus suggested that exogenous expression of Pdx-1 should provide a promising approach for efficiently producing islet-like cells from BMSCs for the future therapeutic use in diabetic patients. | SUN Jiping1,YANG Yujia1,WANG Xiaoli1,SONG Jianhui1 & JIA Yanjie2 1.Department of Pediatrics,Xiang-Ya Hospital,Central-South University,Changsha 410008,China 2.Department of Neurology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052,China | 2006 | Science China(Life Sciences)2006，49，5： | 11 |
| 5 | Surveillance of Childhood Blood Lead Levels in 14 Cities of China in 2004-2006显示文摘Objective To investigate the blood lead level in children aged 0-6 years in urban areas of China. Methods Fourteen cities were selected as sites under surveillance. A total of 44 045 peripheral blood specimens were collected from 2004 to 2006, during which 15 727, 14 737, and 13 584 specimens were tested in 2004, 2005, and 2006, respectively. Tungsten atomizer absorption spectrophotometer was employed to determine blood lead level. Results The geometric mean blood lead level in the tested children was 47.10 μg/L with 10.10% ≥100 μg/L, 46.17 μg/L with 7.78% ≥100 μg/L, and 47.03 μg/L with 7.30% ≥100 μg/L in 2004, 2005, and 2006, respectively. The blood lead levels seemed to tend to rise in parallel with the increase of age of the children and were higher in boys (48.84 μg/L, 47.56 μg/L, and 47.78 μg/L in the 3 respective years) than in girls (45.00 μg/L, 44.53 μg/L, and 46.13 μg/L). Conclusion The blood lead levels in children in cities of China are lower than those in previous national studies, but higher than those in developed countries. Childhood lead poisoning remains a public health problem in China. | SHUAI-MING ZHANG, YAO-HUA DAI1, XIAO-HUA XIE, ZHAO-YANG FAN, ZANG-WEN TAN, AND YAN-FENG ZHANG Department of Early Childhood Development, Capital Institute of Pediatrics, Beijing 100020, China | 2009 | Biomedical and Environmental Sciences2009，22，4： | 10 |
| 6 | Methodological study on real-time three-dimensional echo-cardiography and its application in the diagnosis of complex congenital heart disease显示文摘背景实时三维的回响心动描记法(RT-3DE ) 在过去几年期间使心脏的成像的改进革命化。然而,到目前为止为 RT-3DE 没有标准考试方法。这研究试图建立 RT-3DE 的诊断方法并且在复杂的先天的心疾病(CHD ) 的诊断评估它的申请。有复杂 CHD 的五十个病人被 RT-3DE 与实时 3DE 和 FullVolume 的模式检查的方法。一系列新奇容量的看法与凡·普拉格结合了顺序的部分途径被介绍揭示心的病理学的形态学,它与二维的回响心动描记法(2DE ) 的调查结果相比, angiography 和心脏的外科。在 50patients 的结果,完整的体积的 190 图象获得在几声学的窗口 includingsubcostal 被执行,顶端并且同等不连胸骨的区域。在他们之中,(179/190 )94.2% 图象获得是成功的。最部分的容量的看法能清楚地在 92.6% 成功的图象获得被显示。然而,部分容量的看法不能清楚地在 7.4% 被显示,它主要由于考试地点,不适当的 instrumentmulti 参数背景和在完整的 Volumeacquisitions 捕获的整个心的不够的信息的差的观点条件。作为与外科的调查结果和 angiography 相比, RT-3DE 与单个中庭与大动脉和其它的改正的调换包括 1 在 2 种情况中做了修正到 diagnoses 并且僧帽形劈开。2DE 开始为这 2 个病人做的 diagnoses 是有大动脉和 completeatrio 室的氏族的缺点的调换的两倍插头权利室。结论 RT-3DE 能清楚地由容量的看法通过提供更空间的增进知识的心血管的结构与顺序的部分途径相结合的一系列小说显示复杂 CHD 的病理学的形态学,它为 RT-3DE 诊断提供一个实际方法。 | CHEN Guo-zhen, HUANG Guo-ying, LIANG Xue-cun, MA Xiao-jing, CHEN Wei-da, TAO Zi-yu and LIN Qi-shan Pediatric Heart Center, Children’s Hospital of Fudan University Department of Pediatrics, Shanghai Medical College, Shanghai 200032, China | 2006 | Chinese Medical Journal2006，，14： | 9 |
| 7 | Interaction between en- dogenous nitric oxide and carbon monoxide in the pathogenesis of hypoxic pulmonary hypertension显示文摘The aim of the study was to investigate the in-teraction between nitric oxygenase (NOS)/ nitric oxide (NO) and heme oxygenase (HO)/ carbon monoxide (CO) system in the pathogenesis of hypoxic pulmonary hypertension. On a rat model of hypoxic pulmonary hypertension, the pulmo-nary artery pressure was measured, and NO formation and expression of NOS in pulmonary tissues were examined after treatment with ZnPP-IX, an HO-1 inhibitor. The pulmonary artery pressure, CO formation and expression of HO-1 in pulmonary tissues were examined after treatment with L-NAME, a NOS inhibitor. We found that pulmonary hy-pertension developed after 2-week hypoxia, while the con-centration of NO in the pulmonary tissue homogenates and the expression of NOS in intrapulmonary artery endothelial cells decreased markedly. ZnPP-IX worsened pulmonary hypertension of hypoxic rats. However, it increased endoge-nous production of NO and the expression of NOS obviously. The concentration of CO in the pulmonary tissue homoge-nates and the expression of HO-1 in intrapulmonary artery smooth muscle cells increased markedly with hypoxic pul-monary hypertension. L-NAME worsened pulmonary hy-pertension of hypoxic rats, but inhibited CO formation and HO-1 expression (P < 0.01). The results of this study sug-gested that endogenous NOS/NO and HO/CO systems might interact with each other and therefore play an important regulating role in hypoxic pulmonary hypertension. | SHI Yun, DU Junbao, GUO Zhiliang, ZENG Chaomei & TANG Chaoshu Department of Pediatrics, the First Hospital, Peking University, Beijing 100034, China Correspondence should be addressed to Du Junbao (e-mail: junbaodu@ ht.rol.cn) | 2003 | Chinese Science Bulletin2003，48，1： | 8 |
| 8 | Influence of IFNα-2b and BCG on the release of TNF and IL-1 by Kupffer cells in rats with hepatoma显示文摘INTRODUCTIONKupffer cells are residential macrophages in the liver ,which play a critical role in the maintenance of normal liver function and in immunal surveilance of hepatocellular carcinoma (HCC) and other cancers[1].The biological immune modulants have cancers[2].In our previous studies ,the combined use of biological immune modulants showed better dffects . | Xian Yong Bai~1 Xiu Hong Jia~2 Ling Zhong Cheng~3 Yun Di Gu~3 ~1Department of Histology and Embryology,Binzhou Medical College,Binzhou 256603,Shandong Province,China ~2Departrnent of Pediatrics,Binzhou Medical College,Binzhou 256603,Shandong Province,China ~3Department of Histology and Embryology,Shanghai Medical University,Shanghai 200032,ChinaDr.Xian Yong Bai graduated from the Shanghai Medical University as a postgraduate in 1995,Professor of Histology and Embryology,specialized in hepatic tumor immunology,having 36 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 7 |
| 9 | Treatment of rotaviral gastroenteritis with Qiwei Baizhu powder显示文摘AIM To observe the effects of Qiwei Baizhu Powder ( QWBZP) on rotaviral gastroenteritis in children and in animal models.``METHODS Enrolled patients were divided into two groups, and one group was treated with oral rehydration solution(ORS) and the other treated with oral liquid of QWBZP. Neonate mice were orally infected with 50 μLrotavirus suspension (4 × l0s PFU/mL) and treated with ORS or oral liquid of QWBZP, respectively.``RESULTS Eighty-three cases of rotaviral gastroenteritis treated with QWBZP revealed a better efficacy than that treated with ORS (x2 - 10.8T, P<0.05). The contents of sodium and glucose as well as number of patients with positive human rotavirus antigen in stool in QWBZP group were all less than that in ORS group. In animal models,QWBZP was found effective in treating rotavirus gastroenteritis in neonate NIH mice, as compared with control groups. In QWBZP group, the mortality of infected mice was decreased by 73.3%, the body weight of infected mice was increased, the contents of sodium and glucose as well as number of mice with positive rotavirus antigen in feces were significantly reduced, and the pathological changes such as damage of small intestinal mucosa and villi were also obviously alleviated.``CONCLUSION QWBZP has effects on improving the absorptive function of small intestine, shortening the duration of diarrhea and rotavirus shedding from stool and alleviating the pathological changes of small intestine induced by rotavirus. | Shuang-Teng He~1 Fei-Zhou He~2 Can-Rong Wu~3 Shun-Xiang Li~2 Wei-Xin Liu~4 Yong-Fang Yang~2 Shi-Sheng Jiang~2 Gang He~1 1 Institute of Combined Traditional Chinese and Western Medicine,Xiangya Hospital,Hunan Medical University,Changsha,410008,China2 Hunan Province Academy of Traditional Chinese Medicine,Changsha 410006,China3 Department of Microbiology,Hunan College of Traditional Chinese Medicine,Changsha 410007,China4 Department of Pediatrics,The Second Hospital of Shuangfeng County,Hunan Province,411700,China | 2001 | World Journal of Gastroenterology2001，7，5： | 6 |
| 10 | Retrovirus-mediated delivery of an IL-4 receptor antagonist inhibits allergic responses in a murine model of asthma显示文摘This work reports the investigation of the effect of airway IL-4RA gene transfer by a recombinant retroviral vector on airway inflammation and airway responsiveness in asthmatic mice. The retrovirus-mediated delivery of IL-4RA to the airways of mice inhibited elevations of airway responsiveness and the development of allergic inflammation in asthmatic mice, and regulated the Th1/Th2 balance in OVA-sensitized and -challenged mouse models. This suggests that gene therapy is a therapeutic option for treating and controlling chronic airway inflammation and asthma symptoms. | WANG GuiLan1 & LU JiRong2* 1Department of Pediatrics, Zhongshan Boai Hospital, Zhongshan 528403, China 2Department of Pediatrics, First Hospital, Jilin University, Changchun 130021, China | 2010 | Science China(Life Sciences)2010，53，10： | 6 |
| 11 | Molecular pathogenetic mechanism of maternally inherited deafness显示文摘 | GUAN Min - XinDivision and Program in Human Genetics and Center for Hearing and Deafaess Research,Cincinnati Children’s Hospital Medical Center, and Department of Pediatrics, Universityof Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA | 2003 | 中华耳科学杂志2003，1，3： | 6 |
| 12 | ^(103)Pd-induced apoptosis of proliferative smooth muscle cells in bile ducts of dogs:significance and effects on related genes显示文摘BACKGROUND:With the objective of developing a locally- produced radioactive stent,the present study used in vivo animal experiments to explore apoptosis of proliferative smooth muscle cells resulting from facilitation of the expression of genes caused byγ-radiation in order to prevent bile duct restenosis.We therefore explored the effects and significance ofγ-radiation on the activity of caspase-3,Fas and Bcl-2 genes in apoptosis of proliferative smooth muscle cells in the bile duct walls of dogs. METHODS:Twelve dogs were randomly divided into 2 groups(6 in each group).A postinjury bile duct stenosis model was established and radioactive 103 Pd( 103 palladium) or ordinary bile duct stents were implanted into the bile ducts.HE staining,RT-PCR and immunohistochemistry were used to detect the proliferation and apoptosis of bile duct smooth muscle cells in proliferative endomembrane and the expression of related caspase-3,Bcl-2 and Fas genes. RESULTS:The expression of caspase-3 and Fas genes in the bile duct tissues of dogs with radioactive stents was higher than that of dogs with ordinary stents.There was significant apoptosis of proliferative smooth muscle cells in the bile ducts.The expression of the Bcl-2 gene in the bile duct tissues of dogs with radioactive stents was lower than that in those with ordinary stents.There was significant apoptosis of proliferative smooth muscle cells in the dogs with low Bcl-2 gene expression. CONCLUSIONS:Radiation increases the activity of caspase-3 and Fas genes and is associated with apoptosis. The radioactive 103 Pd stent may facilitate apoptosis of proliferative smooth muscle cells in the bile ducts of dogs by activating these genes.The Bcl-2 gene expression level is correlated with the occurrence of apoptosis and the radiosusceptibility of cells. | Gui-Jin He,Fa-Qiang Yu,Rong Wu,Qin-Yi Gao,Shu-He Xu,Hong Gao, Wei-Guo Jiang,Tao Jiang and Xian-Wei Dai Department of General Surgery,Second Affiliated Hospital(Shengjing Hospital),China Medical University,Shenyang 110004,China,Radiotherapy Center,Second Affiliated Hospital(Shengjing Hospital),China Medical University,Shenyang 110004,China,Pediatrics Surgery Laboratory,Second Affiliated Hospital(Shengjing Hospital),China Medical University,Shenyang 110004,China,Department of Pathology,Second Affiliated Hospital(Shengjing Hospital),China Medical University,Shenyang 110004,China Isotope Unit,First Affiliated Hospital,China Medical University,Shenyang 110001,China and Isotope Research Institute,China Institute of Atomic Energy,Beijing 100050,China | 2007 | Hepatobiliary & Pancreatic Diseases International2007，6，5： | 5 |
| 13 | Early complications after excision with hepaticoenterostomy for infants and children with choledochal cysts显示文摘Objective: To retrospectively study the early compli-cations of excision with hepaticoenterostomy for in-fants and children with choledochal cysts.Methods: We analyzed 16 patients with early posto-perative complications out of 173 patients with con-genital choledochal cysts aged 27 days to 14 years(mean 2.4 years) who had undergone excisional pro-cedures and biliary tract reconstruction.Results: The early complications included bile leak-age (10 patients), abdominal wall dehiscence (3),and hepatic failure, pancreatic juice leakage andpostoperative intussusception (each in 1) respective-ly. Three patients died from bile leakage and 1 frompostoperative hepatic failure. No statistical differen-ces were observed between the procedures of biliarytract reconstruction with jejunal segment interposi-tion hepaticoduodenostomy and Roux-en-Y hepatico-jejunostomy (P>0.75). The morbidity was signifi-cantly higher in infants below 1 year than in children(P<0.005). Prevention and treatment of the com-plications were discussed.Conclusion: Bile leakage and abdominal wall dehis-cence are major early postoperative complications.The morbidity of cholechal cysts is higher in infantsthan in children. Exploratory laparotomy should notbe delayed when biliary leakage with diffuse peritoni-tis appears. The 'tension suture in the fascial spaceof the abdominal wall' is useful to prevent and treatwound dehiscence. | Min-Ju Li Jie-Xiong Feng Qi-Fei Jin From the Department of Pediatric Surgery, Children’s Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China | 2002 | Hepatobiliary & Pancreatic Diseases International2002，1，2： | 5 |
| 14 | Posttraumatic stress disorder after liver transplantation显示文摘BACKGROUND: Liver transplantation can lead to the development of posttraumatic stress disorder (PTSD), but the risk factors associated with this progression are not well understood. To study this syndrome in adult liver transplant recipients, a cross-sectional investigation of 296 recipients at our hospital was carried out between January and June 2010. METHODS: Study participants completed two questionnaires [a PTSD self-rating scale (PTSD-SS) and a validated Chinese version of the Medical Outcomes Study Short Form-36 (SF-36)]. Clinical and demographic data were collected from the records of the Chinese Liver Transplant Registry and via questionnaires. RESULTS: The prevalence of full PTSD and partial PTSD (that met the criteria for 2 of the 3 symptom clusters) was 3.7% and 5.4%, respectively, for all transplant recipients. Significant differences between the recipients with no PTSD, partial PTSD, and full PTSD were found in all SF-36 domains except for physical functioning (P=0.466). In general, domain scores were the highest in the recipients who did not meet the criteria for PTSD and the lowest in the recipients who met the criteria for full PTSD. Greater severity of posttraumatic stress symptoms was correlated with poorer quality of life, especially in the bodily pain (P=0.004), social functioning (P=0.001), role-emotional (P=0.048), and mental health (P<0.001) domains. The model for end-stage liver disease (MELD) scores, complications, and educational status were identified by multiple regression analysis as risk factors for developing PTSD. CONCLUSIONS: PTSD occurred after liver transplantation and was significantly associated with decreased quality of life. Higher MELD scores and complications after transplantation were risk factors that contributed to PTSD, and higher education was a protective factor. | Shu-Guang Jin, Lu-Nan Yan, Bo Xiang, Bo Li, Tian-Fu Wen, Ji-Chun Zhao, Ming-Qing Xu and Jia-Ying Yang Department of Pediatric Surgery and Department of Liver and Vascular Surgery , West China Hospital, Sichuan University, Chengdu 610041, China | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，1： | 5 |
| 15 | Umbilical Cord Blood Lead Levels in Shanghai, China显示文摘This study was designed to determine the cord blood lead (BPb) levels of babies born in one urban area of Shanghai, and to preliminarily identify the demographic, social environment and prenatal factors which have an effect on the cord BPb concentrations. From August to November 1993, umbilical cord blood samples were obtained from 605 live newborns in the Yangpu Maternal and Child Hospital. 257 samples were excluded from measurement because of clotting. In 348 cord samples, the geometric mean of cord BPb levels was 9. 2μg/dl, with a 95 % confidence interval of the mean 8. 86-9. 54 (μg/dl). 142 babies (40. 8 % ) had cord BPb levels of 10μg/dl or greater. As a result of this high percentage of newborns with BPb levels equal to or greater than 10 μg/dl, we estimate that each year in the Shanghal City about 60,000 newborns are at risk for developing neuropsychological deficiencies caused by maternal lead exposure during pregnancy. To investigate the factors affecting cord blood levels, the subjects with levels greater than the 70th percentile (10. 7μg/dl) (n = 104) and less than the 3oth percentile (7. 4μg/dl) (n = 104) were selected to compare the demographic, environ ment and prenatal medical history. Increased BPb levels at birth were associated with maternal passive smoking, a family member being occupationally exposed to lead, proximity to major traffic way, household coal combustion, neighborhood coal combustion, low level of meternal occupations, and the increasing occurrence of having the high lead foodstuff pidan (preserved duck egg) during pregnancy. We conclude that prenatal lead exposure has become an impor tant health issue for young children in | SHEN XIAO-MING YAN CHONG-HUAI GUO DI WU SHENG-MEI LI REN-QIU HUANG HONG AO LI-MING ZHOU JIAN-DE HONG ZHAO-YI XU JI-DE JIN XING-MING AND TANG JUN-MING (Division of Childhood Lead Poisoning Prevention, Shanghai Institute for Pediatric Rese | 1997 | Biomedical and Environmental Sciences1997，10，1： | 4 |
| 16 | Usefulness of ω-3 fatty acid supplementation in addition to mesalazine in maintaining remission in pediatric Crohn's disease: A double-blind, randomized, placebo-controlled study显示文摘AIM: To assess the value of long-chain ω-3 fatty acids (FAs) supplementation in addition to amino-salicylic-acid (5-ASA) in pediatric patients with Crohn's disease (CD).METHODS: Thirty-eight patients (20 males and 18females, mean age 10.13 years, range 5-16 years)with CD in remission were randomized into two groups and treated for 12 mo. Group Ⅰ (18 patients) received 5-ASA (50 mg/kg/d)+ω-3 FAs as triglycerides in gastroresistant capsules, 3 g/d (eicosapentanoic acid, EPA, 400mg/g, docosahexaenoic acid, DHA, 200 mg/g). Group Ⅱ (20 patients) received 5-ASA (50 mg/kg/d)+olive oil placebo capsules. Patients were evaluated for fatty acid incorporation in red blood cell membranes by gas chromatography at baseline 6 and 12 mo after the treatment.RESUJLTS: The number of patients who relapsed at 1year was significantly lower in group Ⅰ than in group Ⅱ(P<0.001). Patients in group Ⅰ had a significant increase in the incorporation of EPA and DHA (P<0.001) and a decrease in the presence of arachidonic acids.CONCLUSION: Enteric-coated ω-3 FAs in addition to treatment with 5-ASA are effective in maintaining remission of pediatric CD. | C Romano S Cucchiara A Barabino V Annese C Sferlazzas SIGENP Italian Study Group of Pediatric Inflammatory Bowel Diseases | 2005 | World Journal of Gastroenterology2005，11，45： | 4 |
| 17 | Mitochondrial DNA Mutations Associated with Aminoglycoside Ototoxicity显示文摘The mitochondrial 12S rRNA has been shown to be the hot spot for mutations associated with both aminoglycoside-induced and non-syndromic hearing loss. Of all the mutations, the homoplasmic A1555G and C1494T mutations at a highly conserved decoding region in the 12S rRNA have been associated with aminoglycoside-induced and non-syndromic hearing loss in many families worldwide. The A1555G or C1494T mutation is expected to form novel 1494C-G1555 or 1494U-A1555 base-pair at the highly conserved A-site of 12S rRNA. These transitions make the secondary structure of this RNA more closely resemble the corresponding region of bacterial 16S rRNA. Thus, the new U-A or G-C pair in 12S rRNA created by the C1494T or A1555G transition facilitates the binding of aminoglycosides, thereby accounting for the fact that the exposure to aminoglycosides can induce or worsen hearing loss in individuals carrying these mutations. Furthermore, the growth defect and impairment of mitochondrial translation were observed in cell lines carrying the A1555G or C1494T mutation in the presence of high concentration of aminoglycosides. In addition, nuclear modifier genes and mitochondrial haplotypes modulate the phenotypic manifestation of the A1555G and C1494T mutations. These observations provide the direct genetic and biochemical evidences that the A1555G or C1494T mutation is a pathogenic mtDNA mutation associated with aminoglycoside-induced and nonsyndromic hearing loss. Therefore, these data have been providing valuable information and technology to predict which individuals are at risk for ototoxicity, to improve the safety of aminoglycoside antibiotic therapy, and eventually to decrease the incidence of deafness. | GUAN Min-Xin Division of Human Genetics and Center for Hearing and Deafness Research, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio 45229, USA Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229, USA | 2006 | Journal of Otology2006，1，2： | 3 |
| 18 | Role of Stem Cell Factor and Its Receptor in the Pathogenesis of Pediatric Aplastic Anemia显示文摘In order to investigate the levels of stem cell factor (SCF) and its receptor c-kit protein and mRNA in pediatric aplastic anemia (AA) and their relevance to the pathogenesis, immunocytochemical and in situ hybridization were utilized to detect the expression of SCF and its receptor c-kit gene protein and mRNA, respectively in 59 children with AA and 51 normal controls. The relationship between SCF and c-kit and the pathogenesis of AA was analyzed subsequently. The results showed that the positive rate of SCF protein and mRNA expression in children with AA was significantly lower than that in healthy controls (P<0.05). However, there was no significant difference in the positive rate of c-kit protein and mRNA expression between children with AA and control group (P>0.05). It was concluded that the expression of SCF is significantly decreased in children with AA, which may be closely associated with the pathogenesis of the AA. c-kit may be unrelated to the development of pediatric AA. Therefore, AA in children may have abnormalities at SCF/c-kit signal transduction levels. | 王军 冯建飞 王伟 胡豫 赵雪莲Department of Pediatrics The Third Affiliated Hospital Zhengzhou University Zhengzhou 450052 China 王宏Department of Pediatrics The Third Affiliated Hospital Zhengzhou University Zhengzhou 450052 China 朱绍先Department of Pediatrics The Third Affiliated Hospital Zhengzhou University Zhengzhou 450052 China | 2005 | Journal of Huazhong University of Science and Technology(Medical Sciences)2005，25，1： | 3 |
| 19 | Diagnosis and treatment of biliary atresia: a retrospective study显示文摘BACKGROUND: Biliary atresia (BA) is the end results of an inflammatory process, which affect the intrahepatic and extrahepatic bile ducts, leading to fibrosis and obliteration of the biliary tract with the development of biliary cirrho- sis. Early diagnosis of BA is difficult, and there is no speci- fic therapy for BA at present. The purpose of this study was to investigate the diagnosis, treatment and postoperative outcome of BA and to explore new diagnostic and thera- peutic strategies. METHODS; Thirty-one children with biliary atresia from our hospital and 201 children with such disease from other hospitals in China were reviewed retrospectively in terms of diagnostic modalities, operative age, operative methods and long-term survival rates after operation. RESULTS : The operative age of the 31 patients varied from 13 to 270 days. It was <60 days in 6 children (19% ), 61-90 days in 14 (45% ), 91-120 days in 8 (26% ), and >120 days in 3 ( 10% ). No children underwent transplantation for BA. Their 1-6 year survival rate on average was 32. 3% (10/31). The early operative rate in China was 12.9% (30/ 232). One child received liver transplantation with a long- term survival rate of 31.9%. CONCLUSIONS; Early recognition of babies with BA is critical for optimal intervention for preventing progressive fibrosis. Clinical presentation, imaging and laparoscopy are helpful in enhancing the early diagnostic rate of BA pa- tients. The long-term survival rates can be obtained after the improvement of operative methods, liver transplanta- tion for children with advanced BA, and development of other medical strategies. | Shao-Tao Tang, Qing-Lan Ruan, Zhi-Qing Cao, Yong-Zhong Mao, Yong Wang and Shi-Wang Li Wuhan, China Department of Pediatric Surgery, Wuhan Union Hos- pital, Huazhong Science and Technology University Wuhan 430022, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 3 |
| 20 | Airborne Pollen Grains Of Afyon, Turkey显示文摘The airborne pollen grains of Afyon have been studied for a two_year period (1999-2000) with a Durham sampler. A total of 14 367 pollen grains belonging to 40 taxa have been identified and recorded with some unidentified ones. Of them, 6 732 were identified in 1999 and 7 635 in 2000. Of the total pollen grains, 69.67% were arboreal, 26.64% non_arboreal and 3.68 % unidentified. The majority of the investigated pollen grains were from Pinus, Gramineae, Cupressaceae, Platanus , Chenopodiaceae/Amaranthaceae, Quercus, Ailanthus, Moraceae, Juglans , Salix, Cedrus and Rosaceae. The highest level of pollen grains was in May. | Adem BICAKCI 1, Süheyla ERGUN 2, Sevcan TATLIDIL 1, Hulusi MALYER 1, Sabri ?ZYURT 2, Ahmet AKKAYA 3, Nihat SAPAN 5 Department of Biology, Faculty of Science, University of Uludag, Bursa, Turkey 2. Department of Biology, Faculty of Science, University of Afyon Kocatepe, Afyon Turkey 3. Department of Pulmonology, Faculty of Medicine, University of Süleyman Demirel, Isparta, Turkey 4. Division of Pediatric Allergy, Faculty of Medicine, University of Uludag, Bursa, Turkey) | 2002 | Acta Botanica Sinica2002，44，11： | 3 |
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