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| 1 | Interleukin-1α, 6 regulate the secretion of vascular endothelial growth factor A, C in pancreatic cancer显示文摘Vascular endothelial growth factor (VEGF, namely VEGF-A) is an angiogenic polypeptide and VEGF-C is a lymphangiogenic polypeptide that has been implicated in cancer growth, invasion and metastasis. Several cytokines and growth factors play an important part in cancer progression. These cytokines and growth factors are the principal mediators of cancer cells-stromal cell interaction , which is critical for invasion of cancer cells to the surrounding tissues and metastatic dissemination to distant organs. In this study, we studied VEGF-A, C expression in cultured human pancreatic cancer cell lines and whether the presence of VEGF-A, C in the cell lines is regulated by cytokines interleukin-lct (EL-1α), and interleukin-6 (IL-6). METHODS: We used Northern blot and Western blot methods to analyze expression of the gene and protein of VEGF-A, C in all 6 tested cell lines (ASPC-1, CAPAN-1, MIA-PaCa-2, PANC-1, COLO-357 and T3M4) respectively. To analyze what is the regulator for this VEGF-A, C expression in pancreatic cancer,we used the reverse transcription -polymerase chain reaction (RT-PCR) method to analyze VEGF-A, C expression in cultured human pancreatic cancer cell lines (CAPAN-1 and COLO-357) under the stimulation with IL-1α (10μg/L) or IL-6 (100 μg/L). RESULTS:Northern blot analysis revealed the presence of the 4.1-kb VEGF-A mRNA transcript and 2.4-kb VEGF-C mRNA transcript in all 6 tested cell lines. Immunoblotting with highly specific anti-VEGF-A, anti-VEGF-C antibody revealed the presence of a molecular weight of 43-kDa VEGF-A protein and 55-kDa VEGF-C protein in all the cell lines. RT-PCR analysis revealed the levels of the VEGF-A and VEGF-C gene were 1-2 fold and a 1-fold increase in the COLO-357 cell line by stimulation with IL-la, however, no effect was found in the CAPAN-1 cell line. The levels of the VEGF-A and VEGF-C gene were 2-5 fold and a 1-fold increase in the CAPAN-1 cell line by stimulation with IL-6, but, no effect was found in the COLO-357 cell line. CONCLUSION:These findings suggested that the expression of VEGF-A, C and their regulation by IL-1α, IL-6 in pancreatic cancer contributes to the lymphatic and distant metastasis and the disease progression. | Department of Hepatobiliary Surgery (Tang RF, Zhang FR, Peng L, Wang SX, Xiao Y and Zhang M) and Department of Dermatology (Wang SX), 4th Hospital, Hebei Medical University, Shijiazhuang 050011, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 6 |
| 2 | Immunotherapy of tumors with autologous tumor-derived heat shock protein preparations显示文摘 | Tamura Y Peng P Liu K Daou M Srivastava PK | | 0，，： | 3 |
| 3 | Characterization of proteolipid protein-peptide-specific CD_4^+ T cell of experimental allergic encephalomyelitis in Biozzi AB/H mice | Yong Peng and Chih pin Liu Department of Neurology, The Third Affiliated Hospital, Hunan Medical University , Changsha 410013, China (Peng Y) Division of Immunology, Beckman Research Institute, City of Hope National M edical Center, Duarte, CA 9 | 2002 | Chinese Medical Journal2002，，4： | 3 |
| 4 | 中国大陆居民对中风的危险因素、征兆、应急处理及信息获取等相关认知的调查研究(英文)显示文摘目的:以中国大陆居民为受访对象,就中风的认知和预防进行问卷调查,以推进预防中风的健康教育活动。方法:选择大陆两个主要城市(武汉和成都)公园、商场及其他公共场所的居民为对象,进行一项有关中风知识标准化的横截面问卷调查,内容包括风险因素、征兆、应急救援和信息获取。结果:846人参与问卷调查,其中55%为男性,45%为女性,平均年龄45.7岁。收集数据表明,28%的受调查居民认为高血压是主要的危险因素,头晕(29%)和麻木(20.2%)是中风常见的征兆。所有数据都显示,当中风时人们采取急救措施是有必要的。家庭/朋友(21%)及媒体(电视占19.5%,报纸占17.7%)是中风信息传播的主要途径。结论:应重视推广中国大陆地区有关中风预防的低成本高效率的教育项目,尤其是中风危险因素、征兆、应急处理和信息获取途径的相关项目。开拓多元化的途径,包括开展促进公众健康的活动,通过医疗专家和医疗设施开展健康教育活动向广大民众宣传中风常识和预防措施。 | Zhenyi LI Peng WANG Alice Y M JONES Elizabeth DEAN | 2015 | 康复学报2015，0，2： | 3 |
| 5 | Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study显示文摘 | Hongjie Yu Joseph T Wu Benjamin J Cowling Qiaohong Liao Vicky J Fang Sheng Zhou Peng Wu Hang Zhou Eric H Y Lau Danhuai Guo Michael Y Ni Zhibin Peng Luzhao Feng Hui Jiang Huiming Luo Qun Li Zijian Feng Yu Wang Weizhong Yang Gabriel M Leung | 2013 | The Lancet2013，，： | 2 |
| 6 | Reaction of N-methyl-2-pyrrolidinone with carbon disulfide显示文摘 | Zong Z M Peng Y L Qin Z H | 2000 | Energy Fuels2000，14，3： | 1 |
| 7 | EGFP expres sion in goat mammary epithelial cells显示文摘 | Zheng Y M An Z X Peng X R | 2006 | Chin J Agri Biotechnol2006，3，1： | 1 |
| 8 | PBDEs in sediments of the Beijiang River,China: levels,distribution,and influence of total organic carbon 显示文摘 | CHEN L G HUANG Y M PENG X C | 2009 | Chemosphere2009，76，2： | 1 |
| 9 | An Institution-based View of International Business Strategy: A Focus on Emerging Eeonomies显示文摘 | PENG M W WANG D YL JIANG Y | 2008 | Journal of International Business Studies2008，39，5： | 1 |
| 10 | Shaping carbon nanotubes and the effects on their electrical and mechanical properties显示文摘 | Wang M S Peng L M Wang J Y | 2006 | Adv Funct Mater2006，16，11： | 1 |
| 11 | Characterization of antioxidant and antiglycation properties and isolation of active ingredients from traditional Chinese medicine显示文摘 | Tang S Y Whiteman M Peng Z F | 2004 | Free Radio Biol Med2004，36，12： | 1 |
| 12 | Managerial ties and firm perform- ance in a transition economy: the nature of a micro-macro link 显示文摘 | PENG M W LUO Y D | 2000 | Academy of Management Jorunal2000，43，3： | 1 |
| 13 | Size distribution of heavy metal aerosols in cooling and spray dryer system显示文摘 | WEY M Y YANG J T PENG C Y | 1999 | Journal of Environmental Engineering1999，125，11： | 1 |
| 14 | Effect of rice bran on the production of different king oyster mushroom strains during bottle cultivation显示文摘 | Peng J T Lee C M Tsai Y E | 2000 | J A_gric Res China2000，49，： | 1 |
| 15 | Establishment of a high frequency plant regeneration system of Rauvolfia verticillata via somatic embryogenesis 显示文摘 | Peng M Liao Z Zheng Y | 2011 | J Med Plant Res2011，5，18： | 1 |
| 16 | Preparation and properties of high purity Mg-Y biomaterials显示文摘 | Peng Q M Huang Y D Zhou L Hort N Kainer K U | | 0，，： | 1 |
| 17 | Synopsis of thechinese species of Begonia ( Begoniaceae), with areappraisal of sectional delimitation 显示文摘 | SHUI Y M PENG C I WU C Y | 2002 | BotanicalBulletin of Academia Sinica2002，43，： | 1 |
| 18 | The prevalence of parasites and pathogens in Asian honeybees Apis cerana in China显示文摘 | Li J L Qin H R Wu J Sadd B M Wang X Evans J D Peng W Chen Y | 2012 | PLoS One2012，7，47： | 1 |
| 19 | Hepatoprotective effect of Phyllanthus in Taiwan on acute liver damage induced by carbon tetrachloride显示文摘 | Lee C Y Peng W H Cheng H Y Chen F N Lai M T | 2006 | The American Journal of Chinese Medicine2006，34，3： | 1 |
| 20 | Mechanism of ischemic tolerance induced by hyperbaric oxygen preconditioning involves upregulation of hypoxia-inducible factor-lalpha and erythropoietin in rats显示文摘 | Gu G J Li Y P Peng Z Y Xu J J Kang Z M Xu W G | 2008 | J Appl Physiol2008，104，： | 1 |
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