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| 1 | Methylation profile of the promoter CpG islands of 31 genes that may contribute to colorectal carcinogenesis显示文摘AIM: To establish the methylation profile of the promoter CpG islands of 31 genes that might play etiological roles in colon carcinogenesis.METHODS: The methylation specific PCR in conjunction of sequendng verification was used to establish the methylationprofile of the promoter CpG islands of 31 genes in colorectal cancer (n = 65), the neighboring non-cancerous tissues (n = 5), colorectal adenoma (n = 8), and normal mucosa (n = 1). Immunohistochemically, expression of 10 genes was assessed on the home-made tissue microarrays of tissues from 58 patients. The correlation of tumor specific changes with each of clinical-pathologic features was scrutinized with relevant statistic tools.RESULTS: In comparison with the normal mucosa of the non-cancer patients, the following 14 genes displayed no tumor associated changes: breast cancer 1, early onset (BRCA1), cadherin 1, type 1, E-cadherin (epithelial) (CDH1),death-associated protein kinase 1 (DAPK1), DNA (cytosine-5-)-methyltransferase 1 (DNMT1), melanoma antigen, family A, 1 (directs expression of antigen MZ2-E) (MAGEA1), tumor suppressor candidate 3 (N33), cyclin-dependent kinase inhibitor 1A (p21, Cipl) (p21^WAF1), cyclin-dependent kinase inhibitor 1B (p27, 10pl) (p27^WAF1), phosphatase and tensin hornolog (mutated in multiple advanced cancers 1) (PTEN), retinoic acid receptor, beta (RAR-, Ras association (RaIGDS/AF-6) domain family 1 C (RASSFIC), secreted frizzled-related protein 1 (SFRP1), tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinfiammatory) (TIMP3),and von HippeI-Lindau syndrome (VHL). The rest 17 targets exhibited to various extents the tumor associated changes.As changes in methylation of the following genes occurred marginally, their impact on the formation of colorectal cancer were trivial: adenomatous polyposis coli (APC) (8%, 5165),Ras association (RaIGDS/AF-6) domain family 1A (RASSFIA) (3%, 2/65) and cyclin-dependent kinase inhibitor 2A,alternated reading frame Co14~) (6%, 4/65). The following genes exhibited moderate changes in rnethylation: O-6rnethylguanine-DNA rnethyltransferase (MGMT) (20%, 13/65),rnutL hornolog 1, colon cancer, nonpolyposis type 2 (E. coli) (hMLH1) (18%, 12/65), cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) P16^NK4a) (10%, 10/65),rnethylated in tumor 1 (MINT1) (15%, 10/65), methylated in tumor 31 (MINT31) (11%, 7/65). The rest changed greatly in the rnethylation pattern in colorectal cancer (CRC): cyclin A1 (cyclin al) (100%, 65/65), caudal type homeobox transcriptdon factor 1 (CDX1) (100%, 65/65), RAR(85%, 55/65), myogenic factor 3 (MYOD1) (69%, 45/65),cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)(p15^INK4b) (68%, 44/65), prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (COX2) (72%, 47/65), cadherin 13, H-cadherin (heart) (CDH13) (65%, 42/65), CAAX box 1 (OO~/) (58%, 38/65),tumor protein p73 (p73) (63%, 41/65) and Wilrns tumor 1 (WT/) (58%, 38/65). However, no significant correlation of changes in rnethylation with any given clinical-pathological features was detected. Furthermore, the frequent changes in rnethylation appeared to be an early phase event of colon carcinogenesis. The in situ expression of 10 genes was assessed by the irnrnunohistochernical approach at the protein level: CDH1, CDH13, COX2, cyclin A1, hMLH1,MGMT, p14^ARF, p73, RAR-, and TIMP3 genes in the context of the rnethylation status in colorectal cancer. No clear correlation between the hyperrnethylation of the promoter CpG islands and the negative expression of the genes was established.CONCLUSION: The methylation profile of 31 genes was established in patients with colon cancer and colorectal adenornas, which provides new insights into the DNA rnethylation mediated mechanisms underlying the carcinogenesis of colorectal cancer and may be of prognostic values for colorectal cancer. | Xiao-UXu JianYu Hong-YuZhang Meng-HongSun JunGu XiangDu Da-RenShi PengWang Zhen-HuaYang Jing-DeZhu | 2004 | World Journal of Gastroenterology2004，10，23： | 43 |
| 2 | Association of cyclooxygenase-2 expression with Hp-cagA infection in gastric cancer显示文摘AIM: To observe the expression of cyclooxygenase-2 (COX-2) and to investigate the association between COX-2expression and infection with cytotoxic-associated gene A( cagA) positive strair Helicobacter pylori ( Hp) in humangastric cancer, and subsequently to provide fresh ideas forthe early prevention of gastric cancer.METHODS: 32 Specimens of gastric cancer andcorresponding adjacent normal gastric mucosa were obtainedfrom patients who had undergone surgical operations ofgastric cancer. All the samples including 1 case of stomachmalignant lymphoma and 31 cases of gastric adenocarcinomawere confirmed by pathology diagnosis. The expression ofCOX-2 in 32 specimens of gastric cancer and correspondingadjacent normal gastric mucosa was quantitativelydetermined and analyzed with Flow Cytometry, and the levelsof COX-2 protein were compared between specimens withcagA+ Hp infection and those without cagA+ Hp infection.The cagA gene in 32 specimens of gastric cancer wasdetected bypolymerase chain reaction (PCR) method.RESULTS: Twenty-seven of 32 (84 %) specimens of gastriccancer showed over-expression of COX-2, compared withthe adjacent normal gastric mucosa. cagA+ gene weredetected from 19 specimens of gastric cancer, but not fromthe other 13 specimens. The levels of COX-2 protein in 19specimens of gastric cancer with cagA+ Hp infection (thenumber of positive cells was 73.82±18.2) were significantlyhigher than those in the 13 specimens without cagA+ Hpinfection (the number of positive cells was 35.92±22.1).CONCLUSION: COX-2 is overexpressed in gastric cancerand cagA+Hp infection could up-regulate the expression ofCOX-2 in gastric cancer in human. There may also existanother way or channel to regulate the expression of COX-2 in gastric cancer in addition to cagA+Hp infection.Therefore, applying COX-2 selective inhibitors could be aneffective and promising way to prevent gastric cancer. | Xiao-LinGuo Li-ErWang Shu-YanDu Chen-LingFan LiLi PengWang YuanYuan | 2003 | World Journal of Gastroenterology2003，9，2： | 31 |
| 3 | Methylation profile of the promoter CpG islands of 14 “drug-resistance”genes in hepatocellular carcinoma显示文摘AIM: To establish the DNA methylation patterns of the promoter CpG islands of 14 'drug-resistance' genes in hepatocellular carcinoma (HCC).METHODS: The methylation specific polymerase chain reaction in conjunction with sequencing verification was used to establish the methylation patterns of the 14 genes in the liver tissues of four healthy liver donors, as well as tumor and the paired non-cancerous tissues of 30 HCC patients.RESULTS: While 11 genes (ATP-binding cassette, sub-family G (WHITE), member 2(ABCG2), activating transcription factor (ATF2), beta-2-microglobulin (B2M), deoxycytidine kinase(DCK, occludin (OCLN, v-raf-1 murine leukemia viral oncogene homolog (RAF/), ralA binding protein 1 (RALBP1),splicing factor (45 kD) (SPF45), S-phase kinase-associated protein 2 (p45) (SKP2), tumor protein p53 (Li-Fraumeni syndrome) (TP53) and topoisomerase (DNA) II beta (TOP2B) maintained the unmethylated patterns, three genes displayed to various extents the hypermethylation state in tumor tissues in comparison with the normal counterparts. The catalase (CAT) was hypermethylated in tumor and the neighboring non-cancerous tissue of one case (3.3%). Both glutathione S-transferase pi (GSTp/) (80%, 24/30 in tumor and 56.7%,17/30 in the paired non-cancerous tissues) and cystic fibrosis transmembrane conductance regulator, ATP-binding cassette (sub-family C, member 7) (CF-FR) (77%, 23/30 in tumor and 50%, 15/30 in the paired non-cancerous tissues) genes were prevalently hypermethylated in HCC as well as their neighboring non-cancerous tissues. No significant difference in the hypermethylation occurrence was observed between the HCC and its neighboring non-cancerous tissues.CONCLUSION: Hypermethylation of promoter CpG islands of both CF-FR and GSTpigenes occurs prevalently in HCC,which may correlate with the low expression of these two genes at the mRNA level and has the profound etiological and clinical implications. It is likely to be specific to the early phase of HCC carcinogenesis. | ShengDing Bang-DongGong JianYu JunGu Hong-YuZhang Zu-BinShang QiFei PengWang Jing-DeZhu | 2004 | World Journal of Gastroenterology2004，10，23： | 14 |
| 4 | Current preventive treatment for recurrence after curative hepatectomy for liver metastases of colorectal carcinoma: A literature review of randomized control trials显示文摘To review the preventive approaches for recurrence after curative resection of hepatic metastases from coloreclal carcinoma, we have summarized all available publications reporting randomized control trials (RCTs) covered in PubMed. The treatment approaches presented above include adjuvant intrahepatic arterial infusion chemotherapy,systemic chemotherapy, neoadjuvant chemotherapy, and immunotherapy. Although no standard treatment has been established, several approaches present promising results, which are both effective and tolerable in posthepatectomy patients. Intrahepatic arterial infusion chemotherapy should be regarded as effective and tolerable and it increases overall survival (OS) and diseasefree survival (DFS) of patients, while 5-fluorouracil-based systemic chemotherapy has not shown any significant survival benefit. Fortunately chemotherapy combined with hepatic arterial infusion and intravenous infusion has shown OS and DFS benefit in many researches. Few neoadjuvant RCT studies have been conduced to evaluate its effect on prolonging survivals although many retrospective studies and case reports are published in which unresectable colorectal liver metastases are downstaged and made resectable with neoadjuvant chemotherapy.Liver resection supplemented with immunotherapy is associated with optimal results; however, it is also questioned by others. In conclusion, several adjuvant approaches have been studied for their efficacy on recurrence after hepatectomy for liver metastases from colorectal cancer (CRC), but multi-centric RCT is still needed for further evaluation on their efficacy and systemic or local toxicities. In addition, new adjuvant treatment should be investigated to provide more effective and tolerable methods for the patients with resectable hepatic metastases from CRC. | PengWang ZhenChen Wen-XiaHuang Lu-MingLiu | 2005 | World Journal of Gastroenterology2005，11，25： | 5 |
| 5 | Morphological variability and genetic diversity in five species of Skeletonema(Bacillariophyta)显示文摘从中国沿海的水的 Skeletonema 的 12 紧张和来自朝鲜的西方海的另一紧张用轻显微镜学(LM ) 被检验,扫描并且传播电子显微镜学(SEM, TEM ) ,并且小子单元(SSU ) rDNA 顺序分析。我们识别了 Skeletonema 的四种:S。marinoi Sarno 和 Zingone, S。subsalsum (A.Cleve ) Bethge, S。dohrnii Sarno 和 Kooistra, S。回归线嗯 Cleve,以及象 Skeletonema 一样种。这些是 S 的第一个记录。marinoi, S。dohrnii 和 S。在中国的 subsalsum。象 Skeletonema 一样种类, MMDL5067,从 Changjiang 河的河口被孤立。这种的词法、种系发生的特征不任何以前出版的 Skeletonema 种类对应于那些。闰的 fultoportula 过程(IFPP ) 有长开的试管,和闰的 rimoportula 过程(IRP ) 在 IFPP 的边界内短、定位。种系发生的分析揭示了与 S 聚类的那 MMDL5630。回归线嗯在 NJ 和 MP,树,而是它的 IRP S 的以前描述的标本不同于那些。回归线嗯。IRP 部分是开的,与在试管的底的一个伸长的洞,并且比 IFPP 长。S 的一些标本。marinoi 和 S。dohrnii 也比 IFPP 有更长管状的 IRP。比作 S 的以前描述的标本。marinoi 和 S。dohrnii,我们识别了的这二个标本有更短的 IRP。我们的结果反映 Skeletonema 分类的歧义和差异。他们为未来鉴定和 Skeletonema 在中国附近在海引起的红潮的管理也是重要的。 | Jinfeng Cheng Yang Li Junrong Liang Yahui Gao PengWang Ho Kin-Chung Xin Lin | 2008 | Progress in Natural Science:Materials International2008，18，11： | 5 |
| 6 | The Arabidopsis phosphatase PP2C49 negatively regulates salt tolerance through inhibition of At HKT1;1显示文摘Type 2C protein phosphatases(PP2Cs)are the largest protein phosphatase family.PP2Cs dephosphorylate substrates for signaling in Arabidopsis,but the functions of most PP2 Cs remain unknown.Here,we characterized PP2 C49(AT3G62260,a Group G PP2C),which regulates Na^(+)distribution under salt stress and is localized to the cytoplasm and nucleus.PP2C49 was highly expressed in root vascular tissues and its disruption enhanced plant tolerance to salt stress.Compared with wild type,the pp2c49 mutant contained more Na^(+)in roots but less Na^(+)in shoots and xylem sap,suggesting that PP2C49 regulates shoot Na^(+)extrusion.Reciprocal grafting revealed a root-based mechanism underlying the salt tolerance of pp2 c49.Systemic Na+distribution largely depends on AtHKT1;1 and loss of function of AtHKT1;1 in the pp2c49 background overrode the salt tolerance of pp2c49,resulting in salt sensitivity.Furthermore,compared with plants overexpressing PP2C49 in the wild-type background,plants overexpressing PP2C49 in the athtk1;1 mutant background were sensitive to salt,like the athtk1;1 mutants.Moreover,protein-protein interaction and two-voltage clamping assays demonstrated that PP2C49 physically interacts with AtHKT1;1 and inhibits the Na^(+)permeability of AtHKT1;1.This study reveals that PP2C49 negatively regulates AtHKT1;1 activity and thus determines systemic Na^(+)allocation during salt stress. | Moli Chu Pengwang Chen Sufang Meng Peng Xu Wenzhi Lan | 2021 | Journal of Integrative Plant Biology2021，63，3： | 5 |
| 7 | STRONG PREDICTOR-CORRECTOR METHODS FOR STOCHASTIC PANTOGRAPH EQUATIONS显示文摘纸为随机的缩放仪方程的近似答案介绍数字计划的一个新班。作为到工具的一种有效技术含蓄的随机的方法,强壮的预言者修正者方法(脉冲编码调制) 被设计处理随机的缩放仪方程的答案的情形模拟。脉冲编码调制是强壮的,与顺序被证明会聚 ? 。随机的缩放仪方程和脉冲编码调制的线性 MS 稳定性在论文被研究。随机的缩放仪方程的 MS-unstability 和脉冲编码调制的 MS 稳定性的足够的条件分别地被获得。数字实验表明这些理论结果。 | Feiyan Xiao PengWang | 2016 | Journal of Computational Mathematics2016，34，1： | 5 |
| 8 | A novel protein-DNA interaction involved with the CpG dinucleotide at -30 upstream is linked to the DNA methylation mediated transcription silencing of the MAGE-A1 gene显示文摘To understand the DNA-methylation mediated gene silencing mechanisms, we analyzed in cell culture of the promoter function of the MAGE-A1 gene, which is frequently demethylated and over-expressed in human hepatocellular carcinoma. We have established the correlation of the DNA methylation of the promoter CpG island with expression status of this gene in a panel of the established liver cancer cell lines. The crucial CpG dinucleotide(s) within the minimal promoter subjected to the control mediated by DNA methylation with profound biological functions was also delineated.Furthermore, a novel sequence-specific DNA-protein interaction at the -30 CpG dinucleotide upstream of the gene was found having a vital part to play in the DNA methylation mediated transcription silencing of the MAGE-A1 gene. Our results would not only provide new insights into the DNA methylation mediated mechanisms over transcription of the MAGE-A1 gene, but also pave the way for further defining the cross-talk among DNA methylation, histone modification and chromatin remodeling in detail. | JieZHANG JianYU JunGU BaoMeiGAO YingJunZHAO PengWANG HongYuZHANG JingDeZHU | 2004 | Cell Research2004，14，4： | 3 |
| 9 | Construction of a novel Shigella live-vector strain co-expressing CS3 and LTB/STm of enterotoxigenic E.coli显示文摘AIM: To construct and evaluate a polyvalent recombinant vaccinie strain Shigella flexneri2a T32 against enterotoxigenic E.coli(ETEC).METHODS: By using a host-plasmid balanced lethal system based on asdgene, a polyvalent recombinant strain was constructed to highly express CS3 and regularly express fusion enterotoxin of LTB subunit and mutant ST (LTB/STm) in a vaccine strain Shigella flexneri 2a T32with specific deletion of asd gene. Fimbria CS3 was observed by immunofluorescence and electron microscopy assay. The security of LTB/STm was examined by ileal loop assay and suckling mouse assay. To evaluate this new candidate vaccine, it was compared with a previous vaccine strain in plasmid and protein level, growth assay and immunogenicity in Balb/c mice.RESULTS: The newly constructed vaccine expressed CS3 and grew better than the previously constructed vaccine except for the lower expression of LTB/STm. Serum IgG and mucosal IgA against CS3, LTB, ST, and host lipopolysaccharide (LPS) were produced after immunization of Balb/c mice by oral route with the new strain. The titers were not significantly different from the Balb/c mice with the previous strain.CONCLUSION: This novel candidate diarrheal vaccine can effectively induce serum and mucosal antibody responsesagainst ETEC and Shigella. | Ji-PingZheng Zhao-ShanZhang Shu-QinLi Xiang-XinLiu Sheng-LingYuan PengWang De-WenZhan Ling-ChunWang Cui-FenHuang | 2005 | World Journal of Gastroenterology2005，11，22： | 2 |
| 10 | The Investigation on the Value of Repeat and Combination Test of ACA and Anti‐β<sub>2</sub>‐GPI Antibody in Women with Recurrent Spontaneous Abortion显示文摘 | Shi HuaBao Xi PengWang Qi DeLin WenDi LiangXu Chuan WeiDing | 2008 | American Journal of Reproductive Immunology2008，，4： | 1 |
| 11 | HOMOTOPY CONTINUATION METHODS FOR STOCHASTIC TWO-POINT BOUNDARY VALUE PROBLEMS DRIVEN BY ADDITIVE NOISES显示文摘在这份报纸,为添加剂噪音驾驶的随机的二点的边界价值问题的 homotopy 继续方法被学习。homotopy 方程的答案的存在被证明。数字计划被构造,错误估计被获得。数字实验在象射击方法那样的另外的通常使用的方法上表明 homotopy 继续方法的有效性。[从作者抽象] | Yanzhao Cao PengWang Xiaoshen Wang | 2014 | Journal of Computational Mathematics2014，32，6： | 1 |
| 12 | Liquid chromatogra- phy-mass spectrometry method for determination of tetram- ethylpyrazine and its metabolite in dog plasma显示文摘 | PengWang Xin Jin Meiling Qi | 2004 | Journal of Chromatography B2004，，813： | 1 |
| 13 | Mercury(Ⅱ) detection by water-soluble photoluminescent ultra-small carbon dots synthesized from cherry tomatoes显示文摘Mercury ions have been considered highly toxic to human health. What would be great is to develop the ionic probes without any toxicities themselves. Here, we report a friendly, highly sensitive mercury(II) ionic probe, watersoluble photoluminescence carbon dots which were synthesized by simply hydrothermal treatment of fresh cherry tomatoes without adding any other reagents. The ultra-small(\1 nm) carbon dots show robust excitation-depended photoluminescence under a wide p H range(4–10) or a strong ionic strength of up to 1 M, and the detection limit of mercury(II) has been determined as low as 18 n M. We envision such water-soluble, biocompatible carbon dots that could be applied to biolabeling, bio-imaging, and biosensing fields. | PengWang Rui-Bo Zhong Ming Yuan Pei Gong Xin-Min Zhao Feng Zhang | 2016 | Nuclear Science and Techniques2016，27，2： | 1 |
| 14 | Millimeter-scale Fuel Cell with onboard fuel and passive control system 显示文摘 | Moghaddam S Pengwang E Lin K Y | 2008 | Journal of Micro Electromechanical Systems2008，17，6： | 1 |
| 15 | Millimeter- Scale Fuel Cell with Onboard Fuel and Passive Control System 显示文摘 | Moghaddam S Pengwang E Lin K Y | 2008 | Journal of Micro Electromechanieal Systems2008，17，6： | 1 |
| 16 | An inorganic-organic proton exchange membrane for fuel cells with a controlled nanoscale pore structure显示文摘 | MOGHADDAM S PENGWANG E JIANG Y | 2010 | Nature Nanotech2010，5，： | 1 |
| 17 | An inorgan-ic-organic proton exchange membrane for fuel cells with acontrolled nanoscale pore structure显示文摘 | Moghaddam S Pengwang E Jiang Y B | 2010 | Nature2010，13，： | 1 |
| 18 | Identifying critical factors that affect the application of information technology in construction management: A case study of China显示文摘The new mode for managing construction projects with information technology(IT)has attracted worldwide attention because it can help managers and workers perform tasks and bring potential benefits,such as high-quality products and accident-free production,effectively.However,the application of IT in site have not achieved expected results because it is faced with many constraints caused by internal factors from enterprises and projects and external factors from the government and environment.Although many relevant studies have discussed the constraints of implementing different IT and devices in the construction industry or site,few articles have specifically focused on identifying and analyzing the indicator system.In this work,we took China as the background,scientifically identified 23 influential factors that affect the implementation of IT in construction management through literature review and expert interviews.Subsequently,questionnaires were issued,and Delphi method was used to obtain empirical data that aimed at four different management fields.Then,an efficient and convenient method called DEMATEL was used to deal with these data.Afterward,the factors were divided into four categories,namely,core,diving,independent,and impact factors.Finally,the similarities and differences of the analysis results from the four fields were compared,and the key factors were identified.Results show that the cross-domain talent ability,concept and value cognition,and organization structure are core factors in all management fields that should be managed first along with the IT innovation ability in the enterprise.The formulation of technical standards and related device and training input are also critical in specific fields.Strategic planning plays a role in macro control and promotion.Data management and application,platform construction,solution,and collaboration have direct impacts on information management.The research results provide suggestions not only for the government to formulate effective policies for IT application and promotion in construction industry,but also for enterprises to take measures in improving management efficiency in the construction site and realizing its substantial benefits. | Meishan JIA Youquan XU Pengwang HE Lingmin ZHAO | 2022 | Frontiers of Engineering Management2022，9，2： | 1 |
| 19 | Smac mimetcs sensitize monstemidial anti-inflanunatory drug-induced apoptosis by promoting caspase-3 mediated cytoehrome c release显示文摘 | Alexander Bank PengWang ChunYingDU | 2008 | Cancer Res2008，68，1： | 1 |
| 20 | THE EXACT RECOVERY OF SPARSE SIGNALS VIA ORTHOGONAL MATCHING PURSUIT显示文摘这份报纸试图经由直角的匹配追求(OMP ) 为稀少的信号的恢复调查足够的条件算法。在无声案例中,我们在场为由 OMP 算法的所有 k 稀少的信号的准确恢复的一个新奇足够的条件,并且证明这个条件是锋利的。在吵闹的案例中,为恢复 k 稀少的信号的支持的一个足够的条件也被介绍。通常,为处于这些足够的条件的限制 isometry 常数(RIC ) 的计算典型地是困难的,因此,我们提供为所有 k 稀少的信号的准确恢复不仅可计算出来而且足够的一个新条件。 | Anping Liao Jiaxin Xie Xiaobo Yang PengWang | 2016 | Journal of Computational Mathematics2016，34，1： | 0 |
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