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| 1 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 2 | Histological and Ultrastructural Features for Proliferation Inhibition by Delivery of Exogenous p27^(Kip1) to Rabbit Models after Glaucoma Filtration Surgery显示文摘Purpose: To investigate postoperative inhibition of cell proliferation in animals treated with exogenous p27Kip1 during an experimental glaucoma filtration surgery (GFS) by histological and ultrastructural examinations. Methods:The primer was designed according to p27Kip1 gene sequence of GenBank..The p27Kip1-expressing adenovirus-mediated Ad-p27 was constructed according to standard techniques..Gene therapy was performed by subconjunctival delivery of Ad-p27,mitomycin C(MMC) and PBS served as controls..Histological and ultastructural changes at surgical sites were observed for 28 days postoperatively. Results: Histologically, evident cellular proliferation induced by Ad-p27 was observed on day 7,.with appreciable filtering cavity. However, thin conjunctival layers were noted, and the number of fibroblasts decreased in the Tenon's capsule on day 14. On day 28, the filtering cavity partially disappeared. The histological features of the animals with MMC delivery were similar to those treated by Ad-p27..In contrast,.in the PBS treatment group, thick conjunctival layers were observed, and the number of goblet cells and fibroblasts, increased markedly..The filtering cavity disappeared at postoperative day 21. Twenty eight day postoperatively,.ultrastructural findings showed that most conjunctival epithelial cells in the Ad-p27 treatment group were under static state,.and that organelles were inactive. The number of goblet cells,.and the secretion of mucin,.were decreased..The amount of fibroblasts also decreased with partial apoptotic cells. The ultrastructural features presented by rabbits with the MMC delivery resembled those treated with Ad-p27. In the PBS treatment group, the number of conjunctival epithelial cells increased; the amount of goblet cells increased as the secretion of mucin was strengthened, and a substantial amount of fibroblasts were observed with active and productive organelles. Conclusion: Ad-p27 inhibits the proliferation of Tenon's capsule fibroblasts at surgical sites, and suppresses the formation of scars, thereby promoting surgical efficacy. | Jiangang Yang Pengrui Sun Lingxiao Zhou Xiaoyan Li Yong Zhang Naixue Sun | 2012 | Eye Science2012，27，3： | 3 |
| 3 | Clinical Evaluation of Traumatic Ciliochoroidal Detachment with Surgical Treatment显示文摘Purpose: To determine the clinical features of traumatic ciliochoroidal detachment(CCD), and to evaluate the surgical outcomes.Methods: We retrospectively reviewed the records of 37 consecutive patients with traumatic CCD who underwent surgical procedures,.including ciliary body suturing,.transscleral cyclophotocoagulation,.and cyclocryopexy..A complete ocular examination was performed at pre-surgery and at periodical post-surgery follow-ups. We compared visual acuity(VA),intraocular pressure.(IOP),.and morphologic changes with UBM among the different surgical procedures at the presurgery and periodical follow-ups.Results:.The mean IOP was 6.62 mmHg, and the median VA was 20 / 200 at baseline..The mean final IOP was 11.03mmHg,.and the final median VA improved to 20 / 50..IOPs were significantly different in post-surgery compared with those at baseline(P=0.000) among the ciliary body suturing,cyclophotocoagulation, and cyclocryopexy groups. However,no significant differences were noted at each follow-up among the 3 groups(P>0.05). The post-surgical morphological figures consisted of complete reattachment, partial reattachment,and the complete detachment. Cyclocryopexy(71.4%), suturing(68.4%), and cyclophotocoagulation(63.6%) produced similar surgical outcomes of the complete reattachment based on UBM images.Conclusion:Prompt treatment and periodic follow-ups are necessary after traumatic CCD, based on accurate dimensions and configuration by UBM. The appropriate choice of surgical procedures is pivotal for an optimal outcome. | Jiangang Yang Qianying Liu Xiaoyan Li Lingxiao Zhou Pengrui Sun XiaoHua Wang | 2013 | Eye Science2013，28，3： | 0 |
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