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| 1 | Reprogramming mature terminally differentiated adipocytes to induced pluripotent stem cells显示文摘Mature adipocytes are terminally differentiated somatic cells. Here, we report the successful generation of induced pluripotent stem(i PS) cells from mouse mature adipocytes by forced expression of six transcription factors(Oct4, Sox2, c-Myc, Klf4, Rarc, and Lrh1) with a piggy Bac transposon-based strategy. The resulting i PS cells were pluripotent as evidenced by the fact that they stained positive for alkaline phosphatase, expressed high levels of key pluripotency markers including Oct4, Nanog, and SSEA1,and remained pluripotent on a 2i media. In vitro differentiation of the i PS cells showed that the cell derivatives of all three germ layers could be readily obtained through formation of embryoid bodies. Most importantly, these adipocytederived i PS cells were capable of producing chimera with high frequencies when reintroduced into early-stage embryos and transmitted through the germ line. This study demonstrates that the new six-factor reprogramming technology facilitates the reset of the terminally differentiated adipocytes to the ground state of pluripotency, enabling us to fully explore the potential of mature adipocytes as a viable cell source for regenerative medicine. | Tao Nie Wei Deng Xuefei Gao Wei Sun Xiaoyan Hui Hong Song Dajiang Qin Aimin Xu Peng Li Pentao Liu Liangxue Lai Donghai Wu | 2015 | Science Bulletin2015，60，20： | 1 |
| 2 | A Highly Efficient Recombineering- Based Method for Generating Conditional Knockout Mutations 显示文摘 | Pentao Liu | 2003 | Genome Research2003，13，： | 1 |
| 3 | The Merlin/NF2 Tumor Suppressor Functions through the YAP Oncoprotein to Regulate Tissue Homeostasis in Mammals显示文摘 | Nailing Zhang Haibo Bai Karen K. David Jixin Dong Yonggang Zheng Jing Cai Marco Giovannini Pentao Liu Robert A. Anders Duojia Pan | 2010 | Developmental Cell2010，，1： | 1 |
| 4 | Delineating nuclear reprogramming显示文摘Nuclear reprogramming is described as a molecular switch,triggered by the conversion of one cell type to another.Several key experiments in the past century have provided insight into the field of nuclear repro-gramming.Previously deemed impossible,this re-search area is now brimming with new findings and developments.In this review,we aim to give a historical perspective on how the notion of nuclear reprogram-ming was established,describing main experiments that were performed,including(1)somatic cell nuclear transfer,(2)exposure to cell extracts and cell fusion,and(3)transcription factor induced lineage switch.Ultimately,we focus on(4)transcription factor induced pluripotency,as initiated by a landmark discovery in 2006,where the process of converting somatic cells to a pluripotent state was narrowed down to four tran-scription factors.The conception that somatic cells possess the capacity to revert to an immature status brings about huge clinical implications including per-sonalized therapy,drug screening and disease model-ing.Although this technology has potential to revolu-tionize the medical field,it is still impeded by technical and biological obstacles.This review describes the effervescent changes in this field,addresses bottle-necks hindering its advancement and in conclusion,applies the latest findings to overcome these issues. | Jolene Ooi Pentao Liu | 2012 | Protein & Cell2012，3，5： | 0 |
| 5 | Pluripotency and its layers of complexity显示文摘Pluripotency is depicted by a self-renewing state that can competently differentiate to form the three germ layers.Different stages of early murine development can be captured on a petri dish,delineating a spectrum of pluripotent states,ranging from embryonic stem cells,embryonic germ cells to epiblast stem cells.Anomalous cell populations displaying signs of pluripotency have also been uncovered,from the isolation of embryonic carcinoma cells to the derivation of induced pluripotent stem cells.Gaining insight into the molecular circuitry within these cell types enlightens us about the significance and contribution of each stage,hence deepening our understanding of vertebrate development.In this review,we aim to describe experimental milestones that led to the understanding of embryonic development and the conception of pluripotency.We also discuss attempts at exploring the realm of pluripotency with the identification of pluripotent stem cells within mouse teratocarcinomas and embryos,and the generation of pluripotent cells through nuclear reprogramming.In conclusion,we illustrate pluripotent cells derived from other organisms,including human derivatives,and describe current paradigms in the comprehension of human pluripotency. | Jolene Ooi Pentao Liu | 2012 | Cell Regeneration2012，1，1： | 0 |
| 6 | Using mouse models to study function of transcriptional factors in T cell development显示文摘Laboratory mice have widely been used as tools for basic biological research and models for studying human diseases.With the advances of genetic engineering and conditional knockout(CKO)mice,we now understand hematopoiesis is a dynamic stepwise process starting from hematopoietic stem cells(HSCs)which are responsible for replenishing all blood cells.Transcriptional factors play important role in hematopoiesis.In this review we compile several studies on using genetic modified mice and humanized mice to study function of transcriptional factors in lymphopoiesis,including T lymphocyte and Natural killer(NK)cell development.Finally,we focused on the key transcriptional factor Bcl11b and its function in regulating T cell specification and commitment. | Peng Li Yiren Xiao Zhixin Liu Pentao Liu | 2012 | Cell Regeneration2012，1，1： | 0 |
| 7 | Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis显示文摘Dear Editor,The cell-cell interactions are fundamental mechanisms of differentiating embryonic tissues.1 We aimed to model human embryonic development in stem-cell-derived organoids.We exploited the time series single-cell and spatial transcriptomics to reveal the gene expression landscape in the development of embryonic tissues and their interactions. | Yiming Chao Yang Xiang Jiashun Xiao Weizhong Zheng Mo R.Ebrahimkhani Can Yang Angela Ruohao Wu Pentao Liu Yuanhua Huang Ryohichi Sugimura | 2023 | Signal Transduction and Targeted Therapy2023，8，7： | 0 |
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