|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Leptin: a multifunctional hormone显示文摘Leptin is the protein product encoded by the obese (ob)gene. It is a circulating hormone produced primarily by the adipose tissue. ob/ob mice with mutations of the gene encoding leptin become morbidly obese, infertile, hyperphagic, hypothermic,and diabetic. Since the cloning of leptin in 1994, our knowledge in body weight regulation and the role played by leptin has increased substantially. We now know that leptin signals through its receptor, OB-R, which is a member of the cytokine receptor superfamily. Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure. Leptin also plays important roles in angiogenesis, immune function, fertility and bone formation. Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment,demonstrating that enhancing or inhibiting leptin’s activities in vivo may have potential therapeutic benefits. | HUANG Lu, CAI LI(Tel: (214)-648-3340 Fax: (214)-648-9191 E-mail: li03@utsw.swmed.edu)(1 Touchstone Center for Diabetes Research1 Departments of Physiology and,2 Departments of Internal MedicineThe University of Texas Southwestern Medical Center5323 H. | 2000 | Cell Research2000，10，2： | 35 |
| 2 | The characteristics of CO_2 assimilation of photosynthesis and chlorophyll fluorescence in transgenic PEPC rice显示文摘With PEPC, PPDK, NADP-ME and PEPC+ PPDK transgenic and untransformed rice (Orysa sativa L.), the activities of related C4 photosynthesis enzymes, the chlorophyll fluorescence parameters, CO2 exchange and other physiological indexes were compared, in which the physiological characteristics of PEPC transgenic rice were mainly studied. The results were as follows: (i) The activities of PEPC in PEPC transgenic rice were 20-fold higher than those in untransformed rice; the light-saturation photosynthetic rates and the carboxylation efficiency of PEPC transgenic rice were increased by 55% and 50% more than those of untransformed rice, respectively, while the CO2 compensation point decreased by 27%. (ii) The PS II photochemical efficiency (Fv/Fm) and photochemical quenching (qp) of transgenic PEPC rice decreased less in comparison with those of untransformed rice after the treatment with high light intensity (3 h) or methyl viologen (MV), a photooxidative reagent, which demonstrated that the tolerance of PEPC | JIAO Demao, LI Xia, HUANG Xueqing, CHI Wei, KUANG Tingyun & Ku SB Maurice1. Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nangjing 210014, China 2. Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China 3. Botany Department, Washington State University, Pullman, WA 99164-4236, USA | 2001 | Chinese Science Bulletin2001，46，13： | 29 |
| 3 | Identification of quantitative trait loci associated with salt tolerance at seedling stage from Oryza rufipogon显示文摘土壤咸度是影响工厂生长和庄稼生产的主要不能生活的压力之一。在现在的学习,在米饭幼苗舞台的盐忍耐用 87 根基因渗入线(IL ) 被评估,它从在精英 indica 栽培变种 Teqing 和普通野米饭(Oryza rufipogon Griff ) 的就职之间的一个十字被导出。实质的变化为包括盐忍耐 20 的四个特点(圣) 被观察,相对的根干燥重量(RRW ) ,干燥重量(RSW ) 和相对总数弄干的相对射击重量(RTW ) 。圣断然显著地所有另外的三个特点地被相关。与这四个特点联系的 15 通常认为的量的特点 loci (QTL ) 的一个总数用单个点的分析被检测,它位于染色体 1, 2, 3, 6, 7, 9 和 10 与解释 phenotypic 变化的 8%26% 。O。在 13 QTL (86.7%) 的导出 rufipogon 的等位基因能在 Teqing 背景改进盐忍耐。影响 RRW, RSW 和 RTW 的四 QTL 簇在染色体上被发现 6, 7, 9 和 10 分别地。在这四 QTL 簇之中,包括三 QTL (qRRW10, qRSW10 和 qRTW10 ) 的主要的簇在染色体 10 的长手臂上在制造者 RM271 附近被发现,并且 O。分别地,当 phenotypic 变化由这解释为三个特点的三单个 QTL 从 19% ~ 26% 变化了时,在这三 loci 的导出 rufipogon 的等位基因与 22.7% , 17.3% 和 18.5% 的添加剂效果增加了 RRW, RSW 和 RTW。另外,几腌容忍的 IL 被选择并且能被用于识别并且利用有利的盐从普通野米饭的容忍的基因并且在盐使用容忍的米饭繁殖编程序。 | Lei Tian,Lubin Tan,Fengxia Liu,Hongwei Cai,Chuanqing Sun State Key Laboratory of Plant Physiology and Biochemistry,National Centre for Evaluation of Agricultural Wild Plant(Rice), Laboratory of Crop Heterosis and Utilization of Ministry of Education Beijing Key Laboratory of Crop Genetic Improvement and Genome of Ministry of Agriculture,Department of Plant Genetics and Breeding,China Agricultural University,Beijing 100193,China | 2011 | Journal of Genetics and Genomics2011，38，12： | 26 |
| 4 | Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions显示文摘AIM: To study the expression of early growth response gene1 (Egr-1 gene) and Bcl-X/L protein and its relationship with the cell apoptosis in human esophageal carcinoma(EC) and precancerous lesions.METHODS: In situ hybridization(ISH), immunohistochemistry (IHC) and TUNEL method were used respectively to detect Egr-1mRNA, Egr-1 protein, apoptosis related-protein Bcl-X/L and cell apoptosis in situ from 66 cases of esophageal squamous cell carcinoma and their upper cut edge and paracancerous mucosa.RESULTS: Egr-1 gene in situ hybridization, Bcl-X/L immunohistochemistry positive products were located in the cytoplasm, while Egr-1 immunohistochemistry and TUNEL positive signal were located in the nuclei. The apoptosis index(AI) and the frequency of apoptosis occurrence were increased gradually from precancerous lesion to cancer (P<0.01) and the expression of Egr-1mRNA and Egr-1 protein in dysplasia was the highest among all specimens (P<0.01).The AI of Egr-1 positive cancer tissues was much higher than that of Egr-1 negative cancer tissues (P<0.01), while the AI of Bcl-X/L positive cancer tissues was much lower than that of Bcl-X/L negative cancer tissues (P<0.01). The AI and Egr-1 expression were not correlated with invasiveness and lymphatic metastasis in EC.CONCLUSION: Cell apoptosis was present through esophageal carcinogenesis. The expression of Egr-1 mRNA and Egr-1 protein were high in precancerous lesion of esophagus. The AI was increased significantly in Egr-1 positive squamous cell carcinoma. Egr-1 might promote apoptotic effect. Egr-1 expression and cell apoptosis may have an important biological significance in esophageal carcinogenesis. | Ming-Yao Wu Ying-Rui Liang Xian-Ying Wu,Department of Pathology,Shantou university Medical College,Shantou 515031,Guangdong Province,China Chu-Xiang Zhuang,Department of physiology,Shantou university Medical College,Shantou 515031,Guangdong Province,China | 2002 | World Journal of Gastroenterology2002，8，6： | 25 |
| 5 | AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis显示文摘There is increasing evidence for considerable interlinking between the responses to heat stress and oxidative stress, and recent researches suggest heat shock transcription factors (Hsfs) play an important role in linking heat shock with oxidative stress signals. In this paper, we present evidence that AtHsfA2 modulated expression of stress responsive genes and enhanced tolerance to heat and oxidative stress in Arabidopsis. Using Northern blot and quantitative RT-PCR analysis, we demonstrated that the expression of AtHsfA2 was induced by not only HS but also oxidative stress. By functional analysis of AtHsfA2 knockout mutants and AtHsfA2 overexpressing transgenic plants, we also demonstrated that the mutants displayed reduced the basal and acquired thermotolerance as well as oxidative stress tolerance but the overexpression lines displayed increased tolerance to these stress. The phenotypes correlated with the expres-sion of some Hsps and APX1, ion leakage, H2O2 level and degree of oxidative injuries. These results showed that, by modulated expression of stress responsive genes, AtHsfA2 enhanced tolerance to heat and oxidative stress in Arabidopsis. So we suggest that AtHsfA2 plays an im-portant role in linking heat shock with oxidative stress signals. | LI Chunguang1,2, CHEN Qijun1, GAO Xinqi1,3, QI Bishu1, CHEN Naizhi1, XU Shouming1, CHEN Jia1 & WANG Xuechen1 1. State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China 2. Department of Industry Engineering, Zhengzhou Institute of Aeronautic Industry Management, Zhengzhou 450015, China 3. College of Life Science, Qufu Normal University, Qufu Shandong 273165, China | 2005 | Science China(Life Sciences)2005，48，6： | 23 |
| 6 | A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes显示文摘Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. Pollen-specific members of calmodulin (CaM), CaM-like (CML), calcium-dependent protein kinase (CDPK) and calcineurin B-like protein (CBL) families were tagged with green fluorescence protein (GFP) and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane (PM), and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes. | Liming Zhou1,2, Ying Fu1,2 and Zhenbiao Yang2,3 (1State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China 2China Agricultural University (CAU)-University of California, Riverside (UCR) Joint Center for Biological Sciences and Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China 3Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA) | 2009 | Journal of Integrative Plant Biology2009，51，8： | 21 |
| 7 | Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo显示文摘BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor metastasis.However,its role in hepatocellular carcinoma(HCC) cells remains unknown.METHODS:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical staining.Apoptosis was evaluated by TUNEL assay.Cell viability and apoptosis were determined by MTT assay and flow cytometry,respectively.Apoptosis-related proteins in SMMC-7721 and HepG2 cells were detected by Western blotting.RESULTS:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of 12-LOX.Baicalein inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent manner.Our in vivo study demonstrated that baicalein also reduced neoplastic nodules.Mechanistically,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of caspase-3.Furthermore,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of 12-LOX.CONCLUSIONS:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment. | Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，2： | 20 |
| 8 | Effect of areca on contraction of colonic muscle strips in rats显示文摘AIM: To investigate the effects of areca on the contractileactivity of isolated colonic muscle strips in rats andmechanism involved.METHODS: Each strip (LMPC, longitudinal muscle ofproximal colon; CMPC, circular muscle of proximal colon;LMDC, longitudinal muscle of distal colon; CMlC, circularmuscle of distal colon. ) was suspended in a tissue chambercontaining 5 mL Krebs solution (37 ℃), bubbledcontinuously with 950 mL@ L-1 O2 and 50 mL@ L-1 CO2 . Themean contractile amplitude (A), the resting tension (T),and the contractile frequency (F) were simultaneouslyrecorded on recorders.RESULTS: Arsca dose dependently increased the meancontractile amplitude, the resting tension of proximal anddistal colonic smooth muscle strips in rats ( P < 0.05). Italso partly increased the contractile frequency of colonicsmooth muscle strips in rats ( P < 0.05). The effects werepartly inhibited by atropine (the resting tension of LMPCdecreased from 0. 44 ± 0. 12 to 0. 17 ± 0.03; the restingtension of LMDC decreased from 0.71 ± 0.14 to 0.03 ± 0.01;the mean contractile amplitude of LMPC increased from -45.8 ± 7.2 to -30.5 ± 2.9; the motility index of CMDC decreasedfrom 86.6± 17.3 to 32.8 ± 9.3; P< 0.05 vs areca), but theeffects were not inhibited by hexamethonium (P> 0.05).CONCLUSION: Areca stimulated the motility of isolatedcolonic smooth muscle strips in rats. The stimulation ofareca might be relevant with M reoeptor partly. | Dong-Ping Xie Lian-Bi Chen,Department of Physiology,Medical College,Shandong University,Jinan 250012,Shandong Province,China Wei Li Song-Yi Qu Tian-Zhen Zheng,Department of Physiology,Lanzhou Medical College,Lanzhou 730000,Gansu Province,China Ying-Li Yang,Northwest Normal University,Lanzhou 730070,Gansu Province,China Yong-Hui Ding Yu-Ling Wei,Drug Control Institute of Gansu Province,Lanzhou 730000,Gansu Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 18 |
| 9 | Significance of changes of gastrointestinal peptides in blood and ileum of experimental spleen deficiency rats显示文摘AIM: To explore the mechanism of spleen deficiency (SD)by studying the relationship of gastro-intestinal peptides level and ileal electro-mechanical activity of SD rats and cold restrain rats.METHODS: (1) spleen deficiency (SD) model was established by feeding Houpou:Zhishi: Dahuang in the ratio of 3:3:2,3 ml/time, for 42 days. (2) The cold restrain stress model:Animals were restrained on grille and placed in a cool water at 18 °C for 3 h. (3) Substance P (SP) and vasoactive intestinal peptide (VIP) levels in all layers of initial part of ileum and blood in rats were measured by radioimmunoassays (RIA)while changes of electric activity and motility in ileum of rats were recorded with electrode and strain gauge.RESULTS: SP levels in ileum and blood of experimental SD rats were significantly higher than that of the control groups (9.89±5.65 vs 1.22±1.18, P<0.005, in ileum; 22.7±3.95 vs6.60±1.47, P<0.001, in blood) while the VIP levels of theSD rats were significantly lower than that of the controls (3.50±2.01 vs9.10±4.91, P<0.05, in ileum; 229.8±62.4 vs560.4±151.3, P<0.001, in blood). As compared with the controls, the average frequency of slow electric waves (21.3±0.96 vs18.2±2.28, P<0.05) and motility (21.5±0.58vs 18±2.65, P<0.005) of SD rats increased obviously and the frequency of fast waves of SD rat also increased. In spontaneous recovery cases, SP levels recovered significantly (compared with the SD groups, 2.99±0.62 vs 9.89±5.65,P<0.001, in ileum; 14.4±4.22 vs 22.7±3.95, P<0.001, in blood) but did not drop to normal. After the SD rats treated with Chinese herbs (Jiawei Sijun zi Tang), SP improved (compared with SD cases, 2.20±1.25 vs9.89±5.65, (P<0.001),in ileum; 10.7±1.88 vs 22.7±3.95, (P<0.001), in blood)and VIP in blood also improved (compared with SD rats,485.7±229.0 vs 229.8±62.4, P<0.01) while the amplitude of motility decreased apparently (compared with the SD rats,0.64±0.096 vs0.89±0.15, P<0.01). The ileal SP levels of cool stress didn't change while the ileal VIP levels of cool stress became significantly lower than that of the control groups (2.87±0.87 vs 9.10±4.91, P<0.01). The blood SP levels of cool stress were significantly higher (15.60±1.83vs 6.60±1.47, P<0.001) whereas the blood VIP levels of cool stress were significantly lower than that of the control group (153.4±70.46 vs 560.4±151.30, P<0.001).CONCLUSION: Changes of SP and VIP levels in initial part of ileum and blood of SD rats and cool stress rats may be closely related to the gastrointestinal motility disorders presented in SD and cool stress rats. the Chinese herbs (Jiawei Sijun zi Tang) currently used have partially therapeutic effect. | Li-Sheng Li Rui-Yao Qu Wei Wang Hua Guo Department of Physiology,Capital University of Medical Sciences,100054,Beijing,China | 2003 | World Journal of Gastroenterology2003，9，3： | 17 |
| 10 | Intracellular compartmentation of CTP synthase in Drosophila显示文摘Compartmentation is essential for the localization of biological processes within a eukaryotic cell.ATP synthase localizes to organelles such as mitochondria and chloroplasts.By contrast,little is known about the subcellular distribution of CTP synthase,the critical enzyme in the production of CTP,a high-energy molecule similar to ATP.Here I describe the identification of a novel intracellular structure con-taining CTP synthase,termed the cytoophidium,in Drosophila cells.I find that cytoophidia are present in all major cell types in the ovary and exist in a wide range of tissues such as brain,gut,trachea,testis,accessory gland,salivary gland and lymph gland.In addition,I find CTP synthase-containing cytoophidia in other fruit fly species.The observation of compartmentation of CTP synthase now permits a broad range of questions to be addressed concerning not only the structure and function of cytoophidia but also the organization and regulation of CTP synthesis. | Ji-Long Liu Medical Research Council Functional Genomics Unit,Department of Physiology,Anatomy and Genetics,University of Oxford,Oxford,OX1 3QX,United Kingdom | 2010 | Journal of Genetics and Genomics2010，37，5： | 16 |
| 11 | Pharmacokinetics of traditional Chinese syndrome and recipe:a hypothesis and its verification(Ⅰ)显示文摘AIM To propose a hypothesis defining theabsorption,distribution,metabolism andelimination of traditional Chinese recipe(TCR)-component in blood of healthy subjects andpatients,and estimate its correctness.METHODS The pharmacokinetics(PK)of samedose of drug was studied in the animal model oftraditional Chinese syndrome(S)and healthyanimals.The classification,terminology,concept and significance of the hypothesis wereset forth with evidence provided in the presentstudy.The hypotheses consisted of traditionalChinese syndrome PK(S-PK)and traditionalChinese recipe PK(R-PK).Firstly,the observedtetramethylpyrazine(TMP)PK in healthy,chronically reserpinized rats(rat model ofspleen deficiency syndrome,RMSDS)andRMSDS treated with Sijunzi decoction(SJZD)forconfirmation were used to verify S-PK; secondly,the ferulic acid(FA)PK in healthy andhigh molecular weight dextran(HMWD)-inducedrabbit model with blood stasis syndrome(RDBSS)was also used to verify S-PK;andlastly,TMP PK parameters in serum of healthyrats after orally taken Ligusticum wallichii(LW),LW and Salvia miltiorrhiza(LW&SM)decoctions were compared to verify R-PK.RESULTS The apparent first-order absorption[Ka,(13.61±2.56)h-1,area under the blooddrug concentration-time curve[AUC,(24.88±9.76)μg.h-1mL-1],maximum drug concentration[Cmax,(4.82±1.23)μg·mL-1]of serum TMP inRMSDS were increased markedly(P<0.05)compared with those[Ka=(5.41±1.91)h-1,AUC=(5.20±2.57)μg·h-1·mL-1,Cmax=(2.33±1.77)μg·mL-1]of healthy rats(HR).Theapparent first-order rate constant for α and βdistribution phase[α=(0.38±0.09)h-1,β=(0.06±0.03)h-1,the apparent first-orderintercompartmental transfer rate constants[K10=(0.24±0.07)h-1,K12=(0.11±0.02)h-1,K21=(0.11±0.02)h-1]of serum TMP in RMSDS weredecreased significantly(P<0.01)comparedwith those[K10=(0.88±0.20)h-1,K12=(1.45±0.47)h-1,K21=(0.72±0.22)h-1]of HR.However,no apparent differences occurredbetween HR and RMSDS treated with SJZD.Theserum FA concentration and its AUC[(5.6690±2.3541)μg·h-1·mL-1] in RMBSS were also higherthan those[AUC=(2.7566±0.8232)μg·h-1·mL-1]of healthy rabbits(P<0.05).The Ka(11.51±2.82)h-1,AUC(0.84±0.17)μg·h-1·mL-1of LW & SM-derived TMP in serum weremuch lower(P<0.05)than those[Ka=(19.58±4.14)h-1,AUC=(1.27±0.26)μg·h-1·mL-1]ofLW-derived TMP in serum after oral decoctions.CONCLUSION The SDS and blood stasissyndrome state could affect significantly thepharmacokinetic parameters of drugs and theabnormal SDS pharmacokinetic parameters couldbe normalized by SJZD.The combination ofChinese medicine in TCR could reciprocallyaffect the pharmacokinetic parameters of othercomponents absorbed into the systemiccirculation.These results support the S-and R-PK hypothesis. | Xi Huang Ping Ren Ai Dong Wen Li Li Wang Li Zhang Feng Gao Laboratory of Clinical Pharmacology of Chinese Medicine,Xijing Hospital,The Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Department of Pharmacy,Xijing Hospital,The Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China Department of Physiology,The Fourth Military Medical University,Xi’an 710032,Shaanxi Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 16 |
| 12 | Enhancement in wheat leaf photophosphorylation and photosynthesis by spraying low concentration of NaHSO_3显示文摘Spraying 1-2 mmol/L NaHSO3 on the leaf of wheat results in enhancement of photosynthesis in leaves for about 3 d. The amount of ATP has been increased and the millisecond delayed light emission of the leaves has been enhanced, showing that the transmembrane proton motive force related to photophosphorylation is increased. Spraying PMS (a cofactor catalyzing cycle photophosphorylation) and NaHSO3 separately or together on the leaves, 20% increase in photosynthesis has been observed in all the treatments. There is no additive effect when a mixture is applied, suggesting that the mechanism for NaHSO3 promotion of photosynthesis is similar to PMS, and both of them enhance the supply of ATP. | WANG Hongwei, WEI Jiamian & SHEN Yungang (SHEN Yun Kang)Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai 200032, China | 2000 | Chinese Science Bulletin2000，45，14： | 15 |
| 13 | Polyphenol Oxidase, Peroxidase and PhenylalanineAmmonium Lyase Induced in Postharvest Peach Fruitsby Inoculation with Pichia membranefaciensor Rhizopus stolonifer显示文摘Rhizopus rot of peach fruits could be significantly suppressed by Pichia membranefaciens.Polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonium-lyase (PAL) activities inducedby inoculation with P. membranefaciens or R. stolonifer were studied in postharvest peach fruits. The activ-ities of PPO and PAL in peaches increased significantly after being inoculated with P. membranefaciens + R.stolonifer by 24 h, the activities maintained at a high level throughout the experiment. Under the condition ofinfected with R. stolonifer alone, activity of PPO and PAL could also increased, but the levels were lowerthan those treated with P. membranefaciens+ R. stolonifer. However, fruits inoculaed with P. membrane-faciens+ R. stolonifer or R. stolonifer alone did not stimulated POD activity. The results suggest that theactivation of these defense enzymes is involved in the action of P. membranefaciens against R. stolonifer. | QIN Guo-zheng, TIAN Shi-ping, LIU Hai-bo and XU Yong(Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany , Chinese Academy of Sciences , Beijing 100093 , P. R. China) | 2002 | Agricultural Sciences in China2002，1，12： | 13 |
| 14 | DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments显示文摘The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors,and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses.Through a detailed analysis of the Arabidopsis thaliana genome,82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS),PI-phosphate kinases (PIPK),phospholipases (PL),inositol polyphosphate phosphatases (IPPase),inositol polyphosphate kinases (IPK),PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK,PLC,PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly,DNA chip technology was employed to study the expression patterns of various isoforms.In total,79 mRNA clones were amplified and used for DNA chip generation. Expression profile analysis was performed using samples that represented multiple tissues or cellular responses. Tested samples included normal leaf,stem and flower tissues,and leaves from plants treated with various hormones (auxin,cytokinin,gibberellin,abscisic acid and brassinosteroid) or environmental factors (temperature,calcium,sodium,drought,salicylic acid and jasmonic acid).Results showed that many PI pathway-related genes were differentially expressed under these experimental conditions.In particular,the different isoforms of each family were specifically expressed in many cases,suggesting their involvement in tissue specificity and cellular responses to environmental conditions. This work provides a starting point for functional studies of the relevant PI-related proteins and may help shed light onto the role of PI pathways in development and cellular responses. | Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-Institute of Molecular Plant Physiology (MPI-MP) on 'Plant Molecular Physiology and Signal Transduction', 300 Fenglin Road, 200032 Shanghai, China | 2004 | Cell Research2004，14，1： | 12 |
| 15 | Overexpression of the tonoplast aquaporin AtTIP5;1 conferred tolerance to boron toxicity in Arabidopsis显示文摘Boron (B) toxicity to plants is responsible for low crop productivity in many regions of the world. Here we report a novel and effective means to alleviate the B toxicity to plants under high B circumstance. Functional characterization of AtTIP5;1, an aquaporin gene, re-vealed that overexpression of AtTIP5;1 (OxAtTIP5;1) in Arabidopsis significantly increased its tolerance to high B toxicity. Compared to wild-type plants, OxAtTIP5;1 plants exhibited longer hypocotyls, accelerated development, increased silique production under high B treatments. GUS staining and quantitative RT-PCR (qRT-PCR) results demonstrated that the expression of AtTIP5;1 was induced by high B concentration treatment. Subcellular localization analysis revealed that the AtTIP5;1-GFP fusion protein was localized on the tonoplast membrane, which was consistent with the prediction based on bioinformatics. Taken together, our results suggest that AtTIP5;1 is in-volved in B transport pathway possibly via vacuolar compartmentation for B, and that overexpression of AtTIP5;1 in plants may provide an effective way to overcome the problem resulting from high B concentration toxicity. | Yongqi Pang, Lijuan Li, Fei Ren, Pingli Lu, Pengcheng Wei, Jinghui Cai, Lingguo Xin, Juan Zhang, Jia Chen, Xuechen Wang State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China | 2010 | Journal of Genetics and Genomics2010，37，6： | 11 |
| 16 | AtMYB103 is a crucial regulator of several pathways affecting Arabidopsis anther development显示文摘Previous reports indicated that AtMYB103 has an important role in tapetum development,callose dissolution,and exine formation in A.thaliana anthers.Here,we further characterized its function in anther development by expression pattern analysis,transmission electron microscopy observation of the knockout mutant,and microarray analysis of downstream genes.A total of 818 genes differentially expressed between ms188 and the wild-type were identified by global expression profiling analysis.Functional classification showed that loss-of-function of AtMYB103 impairs cell wall modification,lipid metabolic pathways,and signal transduction throughout anther development.RNA in situ hybridization confirmed that transcription factors acting downstream of AtMYB103 (At1g06280 and At1g02040) were expressed in the tapetum and microspores at later stages,suggesting that they might have important roles in microsporogenesis.These results indicated that AtMYB103 is a crucial regulator of Arabidopsis anther development. | ZHU Jun1,ZHANG GuoQiang2,CHANG YuHua2,LI XiaoChuan2,YANG Jun2,HUANG XueYong2,YU QingBo2,CHEN Hui3,WU TianLong1* & YANG ZhongNan2* 1College of Agriculture and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China 2College of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200234,China 3Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032,China | 2010 | Science China(Life Sciences)2010，53，9： | 10 |
| 17 | Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells显示文摘AIM: To elucidate molecular mechanism of chemopreventiveefficacies of garlic against human gastric cancer (HGC):METHODS: HGC cell line BGC823 was treated with Allitridi (akind of garlic extract) and Allitridi-treated and parentalBGC823 cDNA librarles were constructed respectively byusing λZAP Ⅱ vector. cDNA Representatinal DifferenceAnalysis (cDNA RDA) was perfonmed using BamH Ⅰ cutting-site and abundant ~DNA messages provided by the Iibrarles.Northern blot analysls was applied to identifythe obtaineddifference prnducts.RESULTS: Two specific cDNA fragments were obtained andcharacterized to be derived from homo sapiens folatereceptorα (FRα) gene and calcyclin gene respectively.Northern blot results showed a 4-fold increase in FRα geneexpression level and 9-fold increase in calcyclin mRNA levelin BGC823 cells after Allilridi treatment for 72 h.CONCLUSION: The method of cDNA RDA based on cDNAlibraries combines the high specificity of cDNA RDA withabundant cDNA messages in cDNA library; this expands theapplication of cDNA library and increases the specificity ofcDNA RDA. Up-regulstion of FRα gene and calcyclin geneexpressions induced by Allitridi provide valuable molecularevidence for theefficacy of garlic in treating HGC as well asother diseases. | Yong Li Jian-Tao Cui Wen-Mei Li You-Yong Lu,Beijing Institute for Cancer Research,Beijing Laboratory of Molecular Oncology,School of Oncology,Peking University,Beijing 100034,China Lin Yang,Department of Gastroenterology,PLA Institute for Digestive Diseases,First Affiliated Hospital of First Military Medical University,Chinese,Guangzhou,510000,Guangdong Province,China Rui-Fang Guo,Department of Physiology,Neimenggu Medical College,Huhehaote,010000,Neimengguzizhiqu,China | 2002 | World Journal of Gastroenterology2002，8，2： | 10 |
| 18 | Morphology and mapping analysis of rice(Oryza sativa L.)clustered spikelets(Cl)mutant显示文摘The rice clustered spikelets (Cl) mutant exhib-its a phenotype that most of branch apical have 2-3 spikelets clustered together. SEM (scanning electron microscope) ob-servation suggested that the Cl gene controlled branch apical development, and influenced the terminal spikelets elonga-tion. The spikelet number was reduced in mutant, indicating that Cl may also have an effect on spikelet number. To map Cl locus, two F2 mapping populations derived from the crosses between the Cl and ZhongHua11, and Cl and ZheFu802 were constructed, respectively. The Cl locus was roughly mapped between two CAPS markers, CK0214 and SS0324. A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl2560, with genetic distances of 0.2 and 2.1 cM, respectively. Then we found a PAC contig spanning Cl locus, the region was delim-ited to 196 kb. This result was useful for cloning of the Cl gene. Allelism test demonstrated that Cl was allelic to Cl2, another rice clustered spikelets mutant. | ZHENG Leiying1, ZHU Xudong2, QIAN Qian2, ZHAO Zhong1, ZHANG Jianjun3, HU Xiaohe1, LIN Hongxuan1,4 & LUO Da1 1. National Key Laboratory of Plant Molecular Genetics, Shanghai In-stitute of Plant Physiology and Ecology, Chinese Academy of Sci-ences, Shanghai 200032, China 2. China National Rice Research Institute, Hangzhou 310006, China 3. Crop Institute of Breeding and Cultivation, Shanghai Agricultural Academy, Shanghai 201106, China 4. Shanghai Anxin Research Foundation Laboratory, Shanghai 200032, China | 2003 | Chinese Science Bulletin2003，48，6： | 10 |
| 19 | Proteomic analysis of mitochondria reveals a metabolic switch from fatty acid oxidation to glycolysis in the failing heart显示文摘This work characterizes the mitochondrial proteomic profile in the failing heart and elucidates the molecular basis of mitochondria in heart failure. Heart failure was induced in rats by myocardial infarction, and mitochondria were isolated from hearts by differential centrifugation. Using two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry, a system biology approach was employed to investigate differences in mitochondrial proteins between normal and failing hearts. Mass spectrometry identified 27 proteins differentially expressed that involved in energy metabolism. Among those, the up-regulated proteins included tricarboxylic acid cycle enzymes and pyruvate dehydrogenase complex subunits while the down-regulated proteins were involved in fatty acid oxidation and the OXPHOS complex. These results suggest a substantial metabolic switch from free fatty acid oxidation to glycolysis in heart failure and provide molecular evidence for alterations in the structural and functional parameters of mitochondria that may contribute to cardiac dysfunction during ischemic injury. | WANG Jun1,2, BAI Ling1,2, LI Jing3, SUN ChaoFeng1,2, ZHAO Jin1, CUI ChangCong1,2, HAN Ke1,2, LIU Yu1,2, ZHUO XiaoZhen1,2, WANG TingZhong1,2, LIU Ping1,2, FAN FenLing1,2, GUAN YouFei3 & MA AiQun1,2 1 Department of Cardiology, First affiliated hospital of medical college of Xi’an Jiaotong University, Xi’an 710061, China 2 Ion Channel Disease Laboratory, Key Laboratory of Environment and Genes Related to Diseases of Education Ministry, Xi’an 710061, China 3 Department of Physiology and Pathophysiology Key Laboratory of Molecular Cardiovascular Sciences of the Ministry of Education, Peking University Health Science Center, Beijing 100083, China | 2009 | Science China(Life Sciences)2009，52，11： | 10 |
| 20 | Insulin improves cardiac myocytes contractile function recovery in simulated ischemia-reperfusion:Key role of Akt显示文摘The present study examined cardiac myocytecontractile and Ca2+ transient responses to insulin duringsimulated ischemia/reperfusion (I/R) and furtherinvestigated the role of protein kinase B (Akt) in the insulin-induced inotropic effect. Ventricular myocytes wereenzymatically isolated from adult Sprague-Dawley rats and perfused with Tyrode solution while electrically field-stimulated. Simulated I/R was induced by perfusing the cells with chemical anoxic solution including sodiumcyanide-sodium lactate for 15 min followed by reperfusionwith normal oxygenated Tyrode solution with or withoutinsulin. It is found that insulin only at concentration as high as 10 IU/L could increase cell shortening (16±5%, P < 0.05) in normal myocytes, whereas it concentration-dependently (0.01—10 IU/L) increased the contraction,the velocity ofshortening/releng- thening and Ca2+ transient in I/Rmyocytes. In addition, insulin treatment (1 IU/L) increasedAkt phosphorylation of I/R cardiomyocytes by 2.4-foldcompared with that of the control (P < 0.01). Mostimportantly, pretreatment with LY 294002, a specificinhibitor of phosphatidylinositol 3′-kinase (PI3-kinase), significantly inhibited both Akt phosphorylation and thepositive inotropic response to insulin in the I/Rcardiomyocytes. These results suggest that insulin exertsdirect positive inotropic effect by increasing Ca2+ transient of cardiomyocytes, which is enhanced in the pathologicalcondition of I/R. Akt activation plays an important role inthe insulin-induced improvement of myocyte contractile function following I/R. | ZHANG Bo, ZHANG Haifeng, FAN Qian, MA Xinliang & GAO Feng Department of Physiology, The Fourth Military Medical University,Xi抋n 710032, China Correspondence should be addressed to Gao Feng (e-mail: fgao@fmmu.edu.cn) | 2003 | Chinese Science Bulletin2003，48，13： | 9 |
您所在的机构暂未开通此部分的功能，请联系管理员