|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Multispecies probiotic protects gut barrier function in experimental models显示文摘AIM:To investigate the effect of the probiotic combination Lactibiane Tolerance?(LT)on epithelial barrier function in vitro and in vivo.METHODS:The effect of the multispecies probiotic LT was assessed on several models of epithelial barrier function both in vitro(in basal and inflammatory conditions)and in vivo[visceral hypersensitivity induced by chronic stress or by colonic perfusion of a fecal supernatant(FSN)from patients with irritable bowel syndrome(IBS)].In vitro,we measured the permeability of confluent T84 cell monolayers incubated with or without LT by evaluating the paracellular flux of macromolecules,in basal conditions and after stimulation with lipopolysaccharide(LPS)or with conditioned medium of colonic biopsies from IBS patients(IBS-CM).In vivo,male C57/Bl6 mice received orally NaCl or LT for 15 d and were submitted to water avoidance stress(WAS)before evaluating visceral sensitivity by measuring the myoelectrical activity of the abdominal muscle and the paracellular permeability with 51Cr-EDTA.Permeability and sensitivity were also measured after colonic instillation of FSN.Tight-junctions were assessed by immunoblotting and TLR-4 expression was evaluated by immunohistochemistry RESULTS:Incubation of T84 cell monolayers with LT in basal conditions had no significant effect on permeability(P>0.05 vs culture medium).By contrast,addition of LT bacterial bodies(LT)completely prevented the LPS-induced increase in paracellular permeability(P<0.01 vs LPS 10 ng/mL(LPS 10);P<0.01 vs LPS 100ng/mL(LPS 100),P>0.05 vs culture medium).The effect was dose dependent as addition of 109 LT bacterial bodies induced a stronger decrease in absorbance than 106 LT(109 LT+LPS 10:-20.1%±13.4,P<0.01vs LPS 10;106 LT+LPS 10:-11.6%±6.2,P<0.01 vs LPS 10;109 LT+LPS 100:-14.4%±5.5,P<0.01 vs LPS 100;106 LT+LPS 100:-11.6%±7.3,P<0.05 vs LPS 100).Moreover,the increase in paracellular permeability induced by culturing T84 cells with conditioned medium of colonic biopsies from IBS patients(IBS-CM)was completely inhibited in the presence of 109 LT(P<0.01 vs IBS-CM).LT also significantly prevented the epithelial disruption induced by intracolonic infusion of fecal supernatant from IBS patients(P<0.01 vs IBS FSN)or water avoidance stress P<0.01 vs WAS)in C57/Bl6 mice and increased the expression of occludin in vitro and in vivo,as assessed by immnunoblotting.The WAS-induced effect on visceral sensitivity was prevented by LT treatment since values obtained for all steps of colorectal distension were significantly(P<0.01)different from the WAS group.Finally,LT downregulated the response mediated through TLR-4 in vitro(decrease in tumor necrosis factorαsecretion in response to LPS:-65.8%for 109 LT and-52.5%for 106LT,P<0.01 vs LPS)and in vivo(inhibition of WAS induced an increase in TLR-4 expression in the LT treated mice colon,P<0.01 vs WAS).CONCLUSION:The probiotic LT mix prevented the disruption to the epithelial barrier induced by LPS,stress or colonic soluble factors from IBS patients and prevented visceral hypersensitivity. | Mylene Nébot-Vivinus Cherryl Harkat Hanene Bzioueche Christel Cartier Raffaella Plichon-Dainese Lara Moussa Helene Eutamene Dorsa Pishvaie Sophie Holowacz Christian Seyrig Thierry Piche Vassilia Theodorou | 2014 | World Journal of Gastroenterology2014，20，22： | 12 |
| 2 | Ad- renocortical incidentaloma with uncertain prog- nosis associated with an inadequately treated congenital adrenal hyperplasia 显示文摘 | Chevalier N Carrier P Piche M | 2010 | Ann En- docrinol (Paris)2010，71，1： | 1 |
| 3 | Minimally invasive nephron-sparing surgery for unilateral Wilms tumor显示文摘 | Piché N Barrieras D | | 0，，7： | 1 |
| 4 | A multiplex PCR assay to characterize Potato virus Y isolates and identify strain mixtures显示文摘 | Loranzen J H Piche L M Gudmestad N C | 2006 | Plant Disease2006，，90： | 1 |
| 5 | Effect of the F129L mutation in Alternaria solani on fungicides affecting mitochondrial respiration显示文摘 | PASCHE J S PICHE L M GUDMESTAD N C | 2005 | Plant Disease2005，89，3： | 1 |
| 6 | Mycoparasitism of the extramatrical phase of Glornus intraradices by Tricboderma harzianum显示文摘 | Rousseau A Benhamou N Chet I Piche Y | 1996 | Phytopathology1996，，86： | 1 |
| 7 | Effect of the F129L mutation in Alternaria solani on fungicides affecting mitochondrial respiration显示文摘 | Pasche J S Piche L M Gudmestad N C | 2005 | Plant Disease2005，89，： | 1 |
| 8 | Random and site-specific mutagenesis of the Helicobacter pylori ferric uptake regulator provides insight into Fur structure-function relationships显示文摘 | Gilbreath J J Pich O Q Benoit S L Besold A N Cha J H Maier R J Michel S L Maynard E L Merrell D S | 2013 | Molecular Microbiology2013，89，2： | 1 |
| 9 | Rationale for heating oxaliplatin for the intraperitoneal treatment of peritoneal carcinomatosis: a study of the effect of heat on intraperitoneal oxaliplatin using a murine model显示文摘 | Piche N Leblond FA Sideris L | 2011 | Ann Surg2011，254，1： | 1 |
| 10 | Minimally invasive nephron-sparing surgery for unilateral Wilms tttmor显示文摘 | Piche N Barrieras D | 2012 | J Pediatr Surg2012，47，7： | 1 |
| 11 | Distinct contributions of β4GalNAcTA and β4GalNAcTB to Drosophila glycosphingolipid biosynthesis显示文摘 | STOLZ A HAINES N PICH A | 2008 | Glycoconj J2008，25，2： | 1 |
| 12 | Increased transcription of mitochondrial genes for complex Ⅰ in human platelets during ageing显示文摘 | Merlo Pich M Raule N Catani L | 2004 | FEBS Lett2004，558，13： | 1 |
| 13 | A multiplex PCR assay to characterize Potato virus Y iso- lates and identify strain mixtures 显示文摘 | Lorenzen J H Piche L M Gudmestad N C | 2006 | Plant Disease2006，90，7： | 1 |
| 14 | Rationale for heating oxaliplatin for the intraperitoneal treatment of peritoneal carcinomatosis: a study of the effect of heat on intraperito- neal oxaliplatin using a murine model显示文摘 | Pich~ N Leblond FA Sid6ris L | 2011 | Ann Surg2011，254，1： | 1 |
| 15 | Effect of the F129L mutation in Altemaria solani on fungicides affect- ing mitochondrial respiration 显示文摘 | PASCHE J S PICHE L M GUDMESTAD N C | 2005 | Plant Disease2005，89，10： | 1 |
| 16 | Synthesis of novel tantalum oxide sub- micrometer hollow spheres with tailored shell thickness显示文摘 | AGRAWAL M PICH A GUPTA S ZAFEIROPOULOS N E SIMON P STAMM M | 2008 | Langmuir2008，24，： | 1 |
| 17 | t3ic~synthesks of pIant volatiles: nature's diversity ~ nd ingen 1 tyi显示文摘 | PICHE~ SKY E NOEL J P DUDAREVA N | 2006 | Science2006，311，5762： | 1 |
| 18 | Assessment of a redox alkaline/iron-chelate absorption proeess for the removal of dilute hydrogen sulfide in air emissions 显示文摘 | Piche S Ribeiro N Bacaoui A | 2005 | Chem Eng Sci2005，60，22： | 1 |
| 19 | 显示文摘 | Agrawal M Pich A Zafeiropoulos N E | 2008 | Colloid Polym Sci2008，286，： | 1 |
| 20 | The transcriptome of human epicardial, mediastinal and subcutaneous adipose tissue in men with coronary artery dis- ease 显示文摘 | GUAUQUE- OLARTE S GAUDREAULT N PICHE M E | 2011 | PLoS One2011，6，19： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员