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| 1 | Cyclophosphamide-induced reproductive toxicity: Beneficial effects of Helichrysum odoratissimum(Asteraceae) in male Wistar rats显示文摘Objective: Cyclophosphamide(CP) is commonly used to treat some cancers, but its clinical efficacy is also linked with testicular toxicity. We investigated the effects of aqueous extract(AE) and methanolic extract(ME) of Helichrysum odoratissimum for reducing CP-induced reproductive toxicity in male rats.Methods: In addition to a normal control(group 1), drugs or vehicles were administered orally to seven groups(n = 5) of rats that had already received 4-weeks of pre-treatment with CP(5 mg/[kgád], per oral administration);group 2 received CP + distilled water(10 m L/[kgád]);group 3 received CP + 5% tween 80(10 m L/[kgád]);group 4 received CP + clomiphene citrate(0.25 mg/[kgád]);groups 5 and 6 received CP + AE(50 and 100 mg/[kgád]) and groups 7 and 8 received CP + ME(50 and 100 mg/[kgád]). Animals were sacrificed on day 15, and body and sexual organ weights, sperm characteristics, testosterone level and testicular histology were evaluated.Results: The CP-treated group showed a significant reduction(P < 0.001) in the body and seminal vesicle weights, testosterone level, sperm count, sperm motility and sperm viability, but elevated(P < 0.001)sperm morphological abnormalities and testicular structure alterations, compared to the control group.Interestingly, these detrimental effects of CP were reversed by treatment with H. odoratissimum extracts.For instance, both extracts and all doses of H. odoratissimum significantly increased the sperm count(P < 0.001), sperm motility(AE, 50 mg/kg, P < 0.05;ME, 50 and 100 mg/kg, P < 0.05) and sperm viability(AE, 50 mg/kg, P < 0.001;ME, 50 and 100 mg/kg, P < 0.001) compared to the CP group. H. odoratissimum also improved plasmatic and intratesticular testosterone levels and prevented histological alterations of the testes.Conclusion: H. odoratissimum might be considered as an alternative drug to alleviate/prevent reproductive damage in cancer patients receiving CP chemotherapy. | Pierre Watcho Ismaelle Rosine Mpeck Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Georges Romeo Bonsou Fozin Pierre Kamtchouing Albert Kamanyi | 2019 | Journal of Integrative Medicine2019，17，5： | 3 |
| 2 | Effects of Ficus asperifolia on normal rat estrus cyclicity显示文摘Objective:To evaluate Ficus asperifolia(Moraceae)(F.asperifolia)effecting on regular estrus cycle of Wistar rats.Methods:Air-dried fruits of F.asperifolia were extracted using water.Prior to the test,vaginal smear was monitored daily for a 3-week period to select females with normal(regular)estrous cycle.Those with regular estrus cycle weighing between 150-170 g were randomized into three sets of 15 animals each.Each set was then divided into three groups:Group 1(control)was orally administered with distilled water(10 mL/kg body weight)once a day for 1 week starting from the proestrus stage.Groups 2 and 3 were respectively treated with 100and 500 mg/kg body weight of the plant aqueous extract.The two other sets of 15 animals each were similarly treated as the first set for 3 weeks and 6 weeks respectively.Estrus cycle pattern was monitored before and during plant extract application whereas lipid profile,ovary,uterus and liver growth indices were determined at the end of each treatment.Results:F.asperifolia did not disrupt(0%)the order of appearance of normal estrus cycle stages,namely,proestrus,estrus,metestrus and diestrus.Short-term treatment(1 week duration)exhibited high frequency of appearance of proestrus and estrus stages while mid-(3 weeks)and long-term(6 weeks)treatments revealed constancy in the frequency of all stages irrespective to animal groups.The plasma and organ lipid profile,as well as ovary,uterus and liver growth remained unchanged when compared to distilled water-treated animals.Following long-term administration of plant extract(6 weeks),no adverse effect was noticed.Conclusions:Our data partially support the use of F.asperifolia in common medicine. | Esther Ngadjui Pierre Watcho Telesphore Benoit Nguelefack Albert Kamanyi | 2013 | Asian Pacific Journal of Tropical Biomedicine2013，3，1： | 3 |
| 3 | Androgenic effect of Mondia whitei roots in male rats显示文摘Aim: To determine the effect of the aqueous extract of Mondia whitei (Periplocaceae) roots on testosterone production and fertility of male rats. Methods: Adult male Wistar rats were used. In the acute study, 20 rats were randomly divided into 5 groups of 4 animals each. Four treated groups were administered orally a single dose of Mondia whitei (400 mg/kg) and the controls received a similar amount of distilled water. One group of animals were sacrificed by cervical dislocation 1, 2, 4 and 6 h after treatment, respectively. The controls were sacrificed at 6 h.Testicular testosterone was determined by radioimmunoassay. In the chronic study, 28 rats were divided at random into 4 groups of 7 animals each: Groups 1, 2 and 3 were given orally the plant extract (400 mg·kg^-1·day^-1) for 2, 4 and 8 days, respectively. The animals of Groups 1 and 2 were sacrificed 24 hours after the last dosing. The controls (Group 4) received the same amount of distilled water for 8 days. The fertility was assessed only in Groups 3 and 4 and after that, the animals were sacrificed and the epididymal sperm density, the serum testosterone and the testicular testosterone and 17β-estradiol were assayed. The serum, testicular and epidydimal protein contents were also determined. Results: In the acute treatment groups, the serum and testicular concentrations of testosterone remained unchanged at all the time points. Chronic treatment for 8 days induced a significant increase in the testicular weight, the serum and testicular testosterone, the testicular protein content and the sperm density (P<0.05-0.01), but did not affect the accessory gland weights, the serum protein contents, the testicular concentration of 17β-estradiol and the fertility compared to the controls. Conclusion: Mondia whitei root extract possesses an androgenic property. (Asian J Androl 2004 Sep; 6: 269-272) | Pierre Watcho Pierre Kamtchouing Selestin D.Sokeng Paul F.Moundipa Justine Tantchou Jean L.Essame Noussithe Koueta | 2004 | Asian Journal of Andrology2004，6，3： | 2 |
| 4 | Endoplasmic reticulum stress contributes to prediabetic peripheral neuropathy显示文摘 | Sergey Lupachyk Pierre Watcho Alexander A. Obrosov Roman Stavniichuk Irina G. Obrosova | 2012 | Experimental Neurology2012，，： | 1 |
| 5 | Evaluation of In Vitro Uterotonic Activities of Fruit Extracts of Ficus asperifolia in Rats显示文摘 | Pierre Watcho Esther Ngadjui Pepin Alango Nkeng-Efouet Telesphore Beno?t Nguelefack Albert Kamanyi | 2011 | Evidence-Based Complementary and Alternative Medicine2011，，： | 1 |
| 6 | Effects of pirimiphos-methyl (an organophosphate insecticide )on the fertility of adult male rats 显示文摘 | Ferdinand Ngoula Pierre Watcho Marie-Chantal Dongmo | 2007 | African Health Sciences2007，7，1： | 1 |
| 7 | Oxidized palm oil impairs reproductive functions and architectures in female rats显示文摘Objective:To evaluate the effects of three oxidized palm oil diets(OPD)on female rat reproductive function.Methods:Forty-four female Wistar rats presenting five consecutive and regular estrous cycles were divided into 4 groups.The rats were fed with:a standard diet,70%of standard diet+30%oxidized palm oil diet(OPD1),OPD1+5 g of boiled yolk egg(OPD2)and OPD1+10%sucrose(OPD3)for 125 days,respectively.During the feeding period,morphometric,estrous cycle,sexual behavior,gestation,biochemical and histomorphometric parameters were evaluated.Results:All OPDs significantly increased abdominal circumference,body mass index and Lee index coupled to an irregularity and lengthening of the estrous cycle.They significantly decreased appetite and consumption behaviours,quantic pregnancy index,fertility rate,implantation sites and index,serum progesterone and high-density lipoprotein levels,increased pre-implantation losses,anti-implantation activities,serum estradiol,triglycerides,total and low-density lipoprotein-cholesterol levels,and impaired brain and ovaries oxidative status.Histomorphometric examinations revealed increases in the number of atresic and primary follicles and decreases in secondary,tertiary,Degraaf,total and corpus luteum follicles in ovaries coupled to a neurodegeneration of hypothalamic anteroventral periventricular neurons in the OPD groups compared to the standard diet group.Conclusions:The three OPDs induce obesity and impair the female reproductive function,especially OPD2 and OPD3.These findings contribute to a better understanding of the adverse effects of palm oil bleaching on the reproductive function in female rats,which could be useful in the management of women with obesity-related sexual dysfunction. | Wankeu-Nya Modeste Kengne Inès T Ateba Benjamin S Bend Fortune E Djeumeni Ornela N HathoDominique T Ngadjui E Moundipa Paul F Massoma DieudonnéL Dongmo Alain B Watcho Pierre | 2023 | Asian pacific Journal of Reproduction2023，12，4： | 0 |
| 8 | Aframomum melegueta prevents the ejaculatory complications of propylthiouracil-induced hypothyroidism in sexually experienced male rats: Evidence from intravaginal and fictive ejaculations显示文摘Objective: Hypothyroidism has been claimed to generate sexual dysfunctions such as ejaculatory disorders. Aframomum melegueta is an aphrodisiac plant with pro-ejaculatory properties. We investigated the protective effects of aqueous extract(AE) and methanolic extract(ME) of A. melegueta on the ejaculatory function of hypothyroid male rats.Methods: Forty sexually experienced male rats were partitioned into 8 groups(5 rats per group) and treated for 28 d as follows: Group 1, Control;Group 2, propylthiouracil(PTU, 10 mg/kg)+ distilled water(DW,10 m L/kg);Group 3, PTU + 5% Tween 80(10 m L/kg);Group 4, PTU + bromocriptine(6 mg/kg);Group 5,PTU + AE(20 mg/kg);Group 6, PTU + AE(100 mg/kg);Group 7, PTU + ME(20 mg/kg), and Group 8,PTU + ME(100 mg/kg). On days 0, 7, 14 and 28 of treatment, each male rat was paired with primed receptive female for measurement of ejaculatory latency time(ELT) and post-ejaculatory interval(PEI) for1.5 h. On day 29, each male rat was urethane-anesthetized and the spinal cord was transected.Thereafter, following urethral/penile stimulations and intravenous injection of dopamine, contractions of the bulbospongiosus muscles and the intraseminal pressure were registered. After these recordings,blood was collected through the catheterization of abdominal artery and plasma was used for thyroidstimulating hormone(TSH), prolactin and testosterone assays.Results: PTU-induced hypothyroidism was characterized by a significant elevation(P < 0.001) of plasmatic TSH and prolactin levels, but a decline(P < 0.001) in plasmatic testosterone, compared to untreated group. ELT, PEI, contractions of the bulbospongiosus muscles and the intraseminal pressure were also altered by PTU treatment. On the contrary, A. melegueta extracts elevated testosterone(AE, 100 mg/kg,P < 0.01;ME, 100 mg/kg, P < 0.05) and decreased prolactin(AE, 100 mg/kg, P < 0.05;ME, 20 mg/kg,P < 0.05) levels, compared to corresponding controls. With regard to DW + PTU group, prolactin concentration was lowered(P < 0.05) in rats administered with bromocriptine. Treatment with A. melegueta extracts significantly prevented the lengthening of ELT(P < 0.05) and PEI(P < 0.001). Hypothyroid state also altered the fictive ejaculation by increasing the latency and decreasing the number and frequency of bulbospongiosus muscle contractions. There was also a decrease in the intraseminal pressure. These alterations were significantly(P < 0.05) alleviated in plant extract-treated groups.Conclusion: This study highlighted the ejaculatory disturbance of hypothyroidism in male rats and its prevention with A. melegueta extracts. | Francois Xavier Kemka Nguimatio Patrick Brice Deeh Defo Modeste Wankeu-Nya Esther Ngadjui Albert Kamanyi Pierre Kamtchouing Pierre Watcho | 2019 | Journal of Integrative Medicine2019，17，5： | 0 |
| 9 | Bersama engleriana对脊髓损伤雄性大鼠体外射精潜力影响的评估显示文摘本研究旨在探讨Bersama engleriana对脊髓损伤雄性大鼠虚构射精潜力的影响及潜在作用机制。在注射和不注射多巴胺(12mg kg^-1)或催产素(0.5UI kg^-1)的两种条件下,将大鼠脊髓横向切断,用氨基甲酸乙酯麻醉,再分别静脉注射从Bersama engleriana枯叶中提取的水提物(100mg kg^-1)和醇提物(100mg kg^-1),记录大鼠海绵体肌的肌电活动。序贯疗法结束后每5分钟机械刺激尿道。按照100mg kg^-1的剂量单独静脉注射水提物和醇提物都不能诱导虚构射精。注射植物提取物和生理盐水(200GL/min)的肌电图记录相似,都不能诱导球海绵体肌收缩。多巴胺(12mg kg^-1)和催产素(0.5UI kg^-1)能诱导快速的节律性收缩,伴有阴茎勃起,有时会有精栓排出。用植物提取物对大鼠进行前处理,多巴胺(12mg kg^-1)和催产素(0.5UI kg^-1)诱导的射精完全消失.序贯疗法结束后每5分钟机械刺激尿道总是能诱导阴茎运动和勃起。Bensama engleriana提取物对脊髓损伤雄性大鼠虚构射精活动的作用通过多巴胺和催产素通道进行调节。Bersama engleriana的这种延长射精潜伏期的作用可用于快速射精病人。 | Pierre Watcho Miguel Carro-Juarez | 2009 | Asian Journal of Andrology2009，11，5： | 0 |
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