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| 1 | Hydrogen peroxide-induced apoptosis of human lens epithelial cells is inhibited by parthenolide显示文摘AIM: To explore the effect of parthenolide on hydrogen peroxide(H_2O_2)-induced apoptosis in human lens epithelial(HLE) cells. METHODS: The morphology and number of apoptotic HLE cells were assessed using light microscopy and flow cytometry. Cell viability was tested by MTS assay. In addition, the expression of related proteins was measured by Western blot assay. RESULTS: Apoptosis of HLE cells was induced by 200 μmol/L H_2O_2, and the viability of these cells was similar to the half maximal inhibitory concentration(IC50), as examined by MTS assay. In addition, cells were treated with either different concentrations(6.25, 12.5, 25 and 50 mol/L) of parthenolide along with 200 μmol/L H_2O_2 or only 50 μmol/L parthenolide or 200 mol/L H_2O_2 for 24 h. Following treatment with higher concentrations of parthenolide(50 μmol/L), fewer HLE cells underwent H_2O_2-induced apoptosis, and cell viability was increased. Further, Western blot assay showed that the parthenolide treatment reduced the expression of caspase-3 and caspase-9, which are considered core apoptotic proteins, and decreased the levels of phosphorylated nuclear factor-κB(NF-κB), ERK1/2 [a member of the mitogen-activated protein kinase(MAPK) family], and Akt proteins in HLE cells. CONCLUSION: Parthenolide may suppress H_2O_2-induced apoptosis in HLE cells by interfering with NF-κB, MAPKs, and Akt signaling. | Xing-Chao Shentu Xi-Yuan Ping Ya-Lan Cheng Xin Zhang Ye-Lei Tang Xia-Jing Tang | 2018 | International Journal of Ophthalmology(English edition)2018，11，1： | 3 |
| 2 | Dystrophia canthorum in Waardenburg syndrome with a novel MITF mutation显示文摘AIM:To reveal a novel MITF gene mutation in Waardenburg syndrome(WS),which is an autosomal dominant inherited neurogenic disorder that consists of various degrees of sensorineural deafness and pigmentary abnormalities in the eyes,hair and skin.METHODS:The genetic analysis of the Chinese family was conducted by whole-exome sequencing,then the results were confirmed by Sanger sequencing.RESULTS:WS is classified into type I to IV,which are identified by the W index,clinical characteristics and additional features.The MITF gene mostly accounts for WS type II.In this study,a de novo heterozygous mutation in the MITF gene,c.638 A>G in exon 7,was identified in the patient diagnosed with WS type I features,as the W index was 2.17(over 2.10),with dystrophia canthorum,congenital bilateral profound hearing loss,bilateral heterochromia irides,premature greying of the hair,and excessive freckling on the face at birth.She also underwent refractive errors and esotropia,reduced pigmentation of the choroid and visible choroid vessels.The mutation was not found in previous studies or mutation databases.CONCLUSION:The novel mutation in the MITF gene,which altered the protein in amino acids 213 from the glutamic acid to glycine,is the genetic pathological cause for WS features in the patient.Those characteristics of this family revealed a novel genetic heterogeneity of MITF in WS,which expanded the database of MITF mutations and offered a possible in correcting the W index value of WS in distinct ethnicities.Moreover,ocular symptoms should be emphasized in all types of WS patients. | Xia-Jing Tang Xi-Yuan Ping Chen-Qi Luo Xiao-Ning Yu Ye-Lei Tang Xing-Chao Shentu | 2020 | International Journal of Ophthalmology(English edition)2020，13，7： | 3 |
| 3 | Development of a Wireless-Controlled LED Array for the Tunable Optogenetic Control of Cellular Activities显示文摘1.Introduction In order to decipher a complex biological process,tools are required to perturb the various players involved to gain information about the important parameters.Optogenetic modules are genetically encoded molecular reagents that,when expressed in cells,allow a specific biological process to be precisely controlled by light in a spatiotemporal manner[1].Optogenetics thus offers cell biologists an unprecedented new way to perturb cellular activities.The application of optogenetic approaches in cellular biology and synthetic biology research has evolved tremendously in the last few years[2–4]. | Yuankai Qi Junye Chen Xuechun Liu Xiaoxu Zhou Jiannan Fan Ping Shentu Olof Idevall-Hagren Yingke Xu | 2018 | Engineering2018，4，6： | 1 |
| 4 | Ocular findings in syndromic gingival fibromatosis: a case study and electronic microscopic investigation of lens显示文摘We report a case of syndromic gingival fibromatosis with notable ocular lesions,bilateral congenital cataracts,esotropia,and high myopia of a 21-year-old male patient from China.The patient was diagnosed with gingival fibromatosis based on his massive gingival overgrowth and histological findings that were consistent with gingival fibromatosis through a gingival biopsy.Lens opacity features were presented and phacoemulsificaion with intraocular lens(IOL)implantation was performed to manage the cataracts in both eyes.Transmission electronic microscopy was used to investigate the ultrastructure of the removed lens tissue.We also review the literature on gingival fibromatosis and briefly summarize the ocular manifestations of this rare disease. | Ye-Lei Tang Xing-Chao Shentu Su-Juan Zhao Xia-Jing Tang Long He Fei-Yun Ping | 2014 | International Journal of Ophthalmology(English edition)2014，7，3： | 0 |
| 5 | Role of Rapamycin and 3-MA in oxidative damage of HLECs caused by two doses of UVB radiation显示文摘Background:This study compared the role of autophagy regulators Rapamycin and 3-MA in oxidative damage and apoptosis of human lens epithelial cells(HLECs)caused by two doses of Ultraviolet Radiation B(UVB).Methods:HLECs were irradiated with UVB,and two doses of UVB damage models were constructed.After treatment with autophagy regulators,cell damage tests such as CCK-8,LDH activity,and Ros detection were performed.Western blotting was used to detect the levels of autophagy-related proteins and apoptosis-related proteins.Quantitative real-time PCR(RT-qPCR)was used to detect the mRNA leve of secondary antioxidant enzymes.Flow cytometry was used to examine cell viability and apoptosis.Finally,the proportion of autophagy and apoptosis was observed by electron microscope.Results:Autophagy inhibitor 3-MA promoted oxidative damage and apoptosis of HLECs at low doses of UVB(5 mJ/cm2),which corresponds to 1.3 h of exposure to sunlight in human eyes.Under the high dose of UVB(50mJ/cm2),which is equivalent to 13 h of exposure to sunlight in human eyes,the autophagy inducer Rapamycin caused more extensive oxidative damage and apoptosis of HLECs.3-MA was able to reduce this damage,indicating that moderate autophagy is necessary for HLECs to cope with mild oxidative stress.For high dose UVBinduced oxidative stress,the use of 3-MA inhibiting autophagy is more beneficial to reduce cell damage and apoptosis.The mechanisms include degradation of damaged organelles,regulation of the expression of antioxidant enzymes HO-1,NQO1,GCS and regulation of apoptosis-related proteins.Conclusions:Autophagy played different roles in HLECs oxidative stress induced by two doses of UVB.It provides new ideas for reducing oxidative damage and apoptosis of HLECs to prevent or delay the progression of agerelated cataract(ARC). | Hao Yang Xiyuan Ping Yilei Cui Sifan Zheng Xingchao Shentu | 2023 | Advances in Ophthalmology Practice and Research2023，3，1： | 0 |
| 6 | The impact of GJA3 SNPs on susceptibility to age-related cataract显示文摘AIM: To determine the association of gap junction protein alpha 3(GJA3) gene tag single-nucleotide polymorphisms(SNPs) with susceptibility to age-related cataract(ARC).METHODS: In total, 486 ARC patients were matched with 500 healthy controls. All the participants underwent complete ophthalmic examinations. Haplotype-tagging SNPs of GJA3 gene were selected from the HapMap Beijing Han Chinese population. Genomic DNA was extracted from the peripheral blood leukocytes of all the subjects.Under three different genetic models: dominant, recessive,and additive, the association between SNPs and ARC was examined. After adjusting for age and sex, the genetic effects of the GJA3 SNPs were evaluated with logistic regression analysis.RESULTS: Four tag GJA3 SNPs(rs6490519, rs9506430,rs9509053, and rs9552089) were included in the present study. None of the SNPs showed a significant relationship with an altered risk of total ARC under the dominant,recessive, or additive models. In the subgroup analysis,rs9506430 had a significant effect on the formation of a posterior subcapsular cataract(P=0.002, OR: 0.227, 95%CI:0.088-0.590) under the recessive model.CONCLUSION: Our study indicates that GJA3 variants may influence the development of posterior subcapsular cataracts. Further studies need to be designed to confirm this possibility. | Xia-Jing Tang Xing-Chao Shentu Ye-Lei Tang Xi-Yuan Ping Xiao-Ning Yu | 2019 | International Journal of Ophthalmology(English edition)2019，12，6： | 0 |
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