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| 1 | Ability of luteinizing hormone releasing hormone-Pseudomonas aeruginosa exotoxin 40 binding to LHRH receptor on human liver cancer cells显示文摘AIM: TO explore the ability of recombinant toxin luteinizinghormone releasing hormone-Pseudomonas aeruginosaexotoxin 40 (LHRH-PE40) and LHRH binding to LHRH receptor(LHRHR) on the membrane surface of human liver cancerHEPG cells.METHODS: LHRH was labeled by using 12sI with enzymaticreaction. The affinity and receptor volume of LHP, H-PE40and LHRH binding to LHRHR on the membrane surface ofhuman liver cancer cells were measured with radioligandreceptor assay.RESULTS: The specific activity of LHRH labeled with 12sIwas 2.7x 104 kBq/l^L, and its radiochemical purity reachedto 99.2-99.7%. The binding of 12sI to LHRH was maximalfor 240 min in the warm cultivation, and this binding wasstabilized. The inhibiting rates of ~2SI-LHRH and LHRH onthe proliferation of human liver cancer HEPG cells werenot significantly different. On the basis of the saturationcurve of ~2SI-LHRH binding to the membrane LHRHR of HEPGcells, ~2SI-LHRH of lx10s cpm was selected for radioligandreceptor assay. The affinity constants (Kd) of LHRH-PE40and LHRH binding to the membrane LHRHR of HEPG cellswere 0.43~0.12 nmol/L and 4.86~ 1.47 nmol/L, respectively,and their receptor volumes were 0.37~0.15 l^mol/g and0.42=I=0.13 l^mol/g, respectively. The binding of LHRH-PE40to the membrane protein of normal liver cells was notobserved.CONCLUSION: The recombinant toxin LHRH-PE40 binding tothe membrane surface of LHRHR of human liver cancer HEPGcells was very strong, while the specific binding of it to normalliver cells was not observed. The results provide an importantexperimental basis for the clinical application of LHRH-PE. | Shou-LiangGong GangZhao Hong-GuangZhao Wen-TianLü Guang-WeiLiu PingZhu | 2004 | World Journal of Gastroenterology2004，10，19： | 8 |
| 2 | Novel mixed polymericmicelles for enhancing delivery of anticaneer drug and over- coming multidrug resistance in tumor cell lines simultaneously显示文摘 | LI Xinru LI Pingzhu ZHANG Yanhui | 2010 | Pharm Res2010，27，10： | 1 |
| 3 | Image-guided intracavitary high-dose-rate brachytherapy for cervix cancer:A single institutional experience with three-dimensional CT-based planning显示文摘 | Brian Wang Alexander Kwon Yun pingZhu | 2009 | Brachytherapy2009，8，2： | 1 |
| 4 | Modelling three‐dimensional non‐linear seismic performance of elevated bridges with emphasis on pounding of girders显示文摘 | PingZhu MasatoAbe YozoFujino | 2002 | Earthquake Engng Struct Dyn2002，，11： | 1 |
| 5 | The research on the interface adhesion comparison of ZnS,SiO2 and Ag thin films deposited by vacuum coating method显示文摘 | PingZhu Hao Ren | 1998 | SPIE1998，3175，： | 1 |
| 6 | Catalysis and deactivation of montmorillonite K10 in the aryl O-glycosylation of glycosyl trichloroacetoimidates显示文摘The catalysis of montmorillonite K10(MK10)for aryl O-glycosylation of glycosyl trichloroacetimidates was investigated.It was found that the catalyst MK10 is deactivated gradually in the recycle glycosylation.The fresh and the deactivated catalysts were characterized by X-ray diffraction(XRD),X-ray photoelectron spectroscopy(XPS),Thermogravimetric analysis(TGA),and N_(2)adsorption-desorption.The results show that the eliminated trichloroacetamide molecule deposits on the MK10,which blocks and poisons the active sites,resulting in the deactivation of the catalyst.The regeneration of the deactivated MK10 by calcination was studied preliminarily. | Xiaoliu LI Xinhao YAN Zhiwei LI Hua CHEN Pingzhu ZHANG | 2010 | Frontiers of Chemical Science and Engineering2010，4，3： | 0 |
| 7 | Fluorescence polarization assay for identification of lox-1 ligand through high throughput screening显示文摘AIM: Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) was identified as special receptor for oxidized lowdensity lipoprotein (oxLDL) in endothelial cells. LOX-1 critically mediated oxLDL stimulating the progression of atherosclerosis. Identification and discovery LOX-1 antagonist to therapy atherosclerosis will be an interested exploration. METHODS: Human LOX-1 was expressed as a His-fusion inbacteria and purified by metal ion affinity and gel | Tian-taiZHANG Zhen-taiHUANG PingZHU Guan-huaDU | 2004 | Acta Pharmacologica Sinica2004，25，11： | 0 |
| 8 | Ultramicroscopic observation on the traumainduced neurodegeneration in the newborn rat brain显示文摘 | HuiyingZhang ZhengruiHe PingZhu JunGu WenchaoLiu LihuaCheng | 2004 | 中国组织化学与细胞化学杂志2004，13，3： | 0 |
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