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| 1 | Nitrative and oxidative DNA damage in intrahepatic cholangiocarcinoma patients in relation to tumor invasion显示文摘AIM: Nitrative and oxidative DNA damage such as 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine(8-oxodG) formation has been implicated in initiation and/or promotion of inflammation-mediated carcinogenesis.The aim of this study is to clarify whether these DNA lesions participate in the progression of intrahepatic cholangiocarcinoma.METHODS: We investigated the relation of the formation of 8-nitroguanine and 8-oxodG and the expression of hypoxia-inducible factor-1α (HIF-1α) with tumor invasion in 37 patients with intra-hepatic cholangiocarcinoma.RESULTS: Immunohistochemical analyses revealed that 8-nitroguanine and 8-oxodG formation occurred to a much greater extent in cancerous tissues than in non-cancerous tissues. HIF-1α could be detected in cancerous tissues in all patients, suggesting low oxygen tension in the tumors.HIF-1α expression was correlated with inducible nitric oxide synthase (iNOS) expression (r= 0.369 and P = 0.025)and 8-oxodG formation (r = 0.398 and P = 0.015).Double immunofluorescence study revealed that iNOS and HIF-1α co-localized in cancerous tissues. Notably, the formation of 8-oxodG was correlated significantly with lymphatic invasion (r = 0.386 and P= 0.018). Moreover, 8-nitroguanine and 8-oxodG in non-cancerous tissues were associated significantly with neural invasion (P = 0.042and P = 0.026, respectively). These results suggest that reciprocal activation between HIF-1α and iNOS mediates persistent DNA damage, which induces tumor invasiveness via mutations, resulting in poor prognosis.CONCLUSION: The formation of 8-nitroguanine and 8-oxodG plays an important role in multiple steps of genetic changes leading to tumor progression, including invasiveness. | Somchai Pinlaor Banchob Sripa Ning Ma Yusuke Hiraku Puangrat Yongvanit Sopit Wongkham Chawalit Pairojkul Vajarabhongsa Bhudhisawasdi Shinji Oikawa Mariko Murata Reiji Semba Shosuke Kawanishi | 2005 | World Journal of Gastroenterology2005，11，30： | 9 |
| 2 | Annexin A1: A new immunohistological marker of cholangiocarcinoma显示文摘AIM: To evaluate a new immunohistological marker, annexin A1 (ANXA1), in cholangiocarcinoma (CCA) and hepatocellular carcinoma (HCC). METHODS: Expression of ANXA1 protein was investigated in liver tissues from patients with CCA and HCC by immunohistochemistry. Its expression on differences stages of tumor development was investigated in hamster CCA tissues induced by Opisthorchis viverrini and N -nitrosodimethylamine. Moreover, mRNA expression of ANXA1 was assessed in CCA cell lines by quantitative real-time polymerase chain reaction and silencing of ANXA1 gene expression using small interfering RNA. RESULTS: In human CCA tissue arrays, immunohistochemical analysis revealed that the positive expression of ANXA1 was 94.1% (64/68 cases) consisting of a high expression (66.2%, 45/68 cases) and a low expression (33.8%, 23/68 cases). However, expression of ANXA1 protein was negative in all histologic patterns for HCC (46/46 cases) and healthy individuals (6/6 cases). In hamster with opisthorchiasis-associated CCA, the expression of ANXA1 was observed in the cytoplasm of inflammatory cells, bile duct epithelia and tumor cells. Grading scores of ANXA1 expression were significantly increased with tumor progression. In addition, mRNA expression of ANXA1 significantly increased in all of the various CCA cell lines tested compared to an immortalized human cholangiocyte cell line (MMNK1). Suppressing the ANXA1 gene significantly reduced the matrix metalloproteinase (MMP) 2 and MMP9, and transforming growth factor-β genes, but increased nuclear factor-kB gene expression. CONCLUSION: ANXA1 is highly expressed in CCA, but low in HCC, suggesting it may serve as a new immunohistochemical marker of CCA. ANXA1 may play a role in opisthorchiasis-associated cholangiocarcinogenesis. | Nuttanan Hongsrichan Rucksak Rucksaken Yaovalux Chamgramol Porntip Pinlaor Anchalee Techasen Puangrat Yongvanit Narong Khuntikeo Chawalit Pairojkul Somchai Pinlaorr | 2013 | World Journal of Gastroenterology2013，19，16： | 4 |
| 3 | Cathepsin F cysteine protease of the human liver fluke, Opisthorchis viverrini 显示文摘 | PINLAOR P KAEWPITOON N LAHA T | 2009 | PLoS Negl Trop Dis2009，3，3： | 1 |
| 4 | Oxidative and nitrative DNA damage in animals and patients with in- flammatory diseases in relation to inflammation-related carcinogenesis显示文摘 | Kawanishi S Hiraku Y Pinlaor S | 2006 | Biol Chem2006，387，4： | 1 |
| 5 | Cathepsin F eysteine protease of the human liver fluke, Opisthorchis viverrini 显示文摘 | Pinlaor P Kaewpitoon N Laha T | 2009 | PLoS Negl Trop Dis2009，3，3： | 1 |
| 6 | Oxidative and nitrative DNA damage in animals and patients with inflammatory disease in relation to inflammation-related carcinogenesis显示文摘 | Kawanishi S Hiraku Y Pinlaor S | 2006 | Biological Chemistry2006，387，4： | 1 |
| 7 | Circulating CD14(+) CD16(+) monocyte levels predict tissue invasive character of cholangiocarcinoma显示文摘 | SubimerbC PinlaorS LulitanondV | 2010 | Clin Exp Immunol2010，161，3： | 1 |
| 8 | Curcumin induces a nuclear factor erythroid 2 related factor 2-driven response against oxidative and nitrative stress after praziquantel treatment in liver fluke-infected hamsters显示文摘 | Charoensuk L Pinlaor P Prakobwong S | | 0，，： | 1 |
| 9 | 8-nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini显示文摘 | Pinlaor S Yongvanit P Hiraku Y | 2003 | Biochemical and Biophysical Research Communications2003，309，3： | 1 |
| 10 | Oxidative and nitrative DNA damage in animals and patients with inflammatory diseases in relation to inflammation-related eareinogenesis显示文摘 | Kawanishi S Hiraku Y Pinlaor S | 2006 | Biol Chem2006，387，4： | 1 |
| 11 | iNOS-dependent DNA damage via NF-kappa B expression in hamsters infected with opisthorchis viverrini and its suppression by the antihel minthic drug praziquantel显示文摘 | Pinlaor S Hiraku Y Yongvanit P | 2006 | Int J Cancer2006，119，5： | 1 |
| 12 | PCR diagnosis of pneumocystis carinii on sputum and bronchodveolar lavage samples in immuno comprom ised patients显示文摘 | Pinlaor S Mootsikapun P Pinlaor P | 2004 | Parasitol Res2004，94，3： | 1 |
| 13 | Detection of opportunistic and non-opportunistic intestinal parasites and liver flukes in HIV- positive and HIV-negative subjects显示文摘 | Pinlaor S Mootsikapun P Pinlaor P | 2005 | Southeast Asian J Trop Med Pub Hlth2005，36，4： | 1 |
| 14 | Oxidative and nitra-tive DNA damage in animals and patients with inflammatory diseases in relation to inflammation-related carcinogenesis显示文摘 | Kawanishi S Hiraku Y Pinlaor S | 2006 | Biol Chem2006，387，： | 1 |
| 15 | 8-nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini 显示文摘 | Pinlaor S Yongvanit P Hiraku Y | 2003 | Biochem Biophys Res Commun2003，309，3： | 1 |
| 16 | Mechanism of NO-mediated oxida- tive and nitrative DNA damage in hamsters infected with Opisthorehis viverrini:a model of inflammation-mediated carcinogenesis 显示文摘 | Pinlaor S Hiraku Y Ma N | 2004 | Nitric Oxide2004，11，2： | 1 |
| 17 | Repeated infection with Opisthorchis vivelrini induces accumulation of 8-nitroguanine and 8-oxo-7,8-di- hydro-2' -deoxyguanine in the bile duct of hamsters via inducible ni- tric oxide synthase 显示文摘 | Pinlaor S Ma N Hiraku Y | 2004 | Carcinogenesis2004，25，8： | 1 |
| 18 | 2013,alpha-tocopherol and lipid profiles in plasma and the expression of alpha-tocopherol-related molecules in the liver of Opisthorchis viverrini-infected hamsters显示文摘 | Laothong U Pinlaor P Boonsiri P | 2013 | Parasitology International2013，62，： | 1 |
| 19 | PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immunocompromised patients 显示文摘 | Pinlaor S Mootsikapun P Pinlaor P | 2004 | Parasitol Res2004，94，3： | 1 |
| 20 | Lipid peroxidation and etheno DNA adducts in white blood cells of liver fluke-infected patients: Protection by plasma alpha-tocopherol and praziquantel 显示文摘 | Dechakhamphu S Pinlaor S Sitthithawom P | 2010 | Cancer Epidemiology Biomarkers & Prevention2010，19，1： | 1 |
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