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| 1 | 急性缺血性卒中患者早期处理指南:美国心脏协会/美国卒中协会对医疗专业人员的指南(续前)显示文摘7静脉溶栓
7.1静脉rtPA
急性卒中的静脉溶栓治疗现已得到普遍接受。美国FDA于1996年批准静脉应用rtPA,部分基于NINDSrtPA卒中试验结果。 | Edward C. Jauch Jeffrey L. Saver Harold P. Adams Askiel Bruno J.J. (Buddy) Connors Bart M. Demaerschalk Pooja Khatri Paul W. McMullan Adnan I. Qureshi Kenneth Rosenfield Phillip A. Scott Debbie R. Summers David Z. Wang Max Wintermark Howard Yonas 李海峰(译) 岳耀先(译) 王琦(译) 张贤军(译) 丁晓君(译) | 2013 | 国际脑血管病杂志2013，21，8： | 975 |
| 2 | Developmental dysplasia of the hip in the newborn: A systematic review显示文摘Developmental dysplasia of the hip(DDH) denotes a wide spectrum of conditions ranging from subtle acetabular dysplasia to irreducible hip dislocations. Clinical diagnostic tests complement ultrasound imaging in allowing diagnosis, classification and monitoring of this condition. Classification systems relate to the alpha and beta angles in addition to the dynamic coverage index(DCI). Screening programmes for DDH show considerable geographic variation; certain risk factors have been identified which necessitate ultrasound assessment of the newborn. The treatment of DDH has undergone significant evolution, but the current gold standard is still the Pavlik harness. Duration of Pavlik harness treatment has been reported to range from 3 to 9.3 mo. The beta angle, DCI and the superior/lateral femoral head displacement can be assessed via ultrasound to estimate the likelihood of success. Success rates of between 7% and 99% have been reported when using the harness to treat DDH. Avascular necrosis remains the most devastating complication of harness usage with a reported rate of between 0% and 28%. Alternative non-surgical treatment methods used for DDH include devices proposed by LeD amany, Frejka, Lorenz and Ortolani. The Rosen splint and Wagner stocking have also been used for DDH treatment. Surgical treatment for DDH comprises open reduction alongside a combination of femoral or pelvic osteotomies. Femoral osteotomies are carried out in cases of excessive anteversion or valgus deformity of the femoral neck. The two principal pelvic osteotomies most commonly performed are the Salter osteotomy and Pemberton acetabuloplasty. Serious surgical complications include epiphyseal damage, sciatic nerve damage and femoral neck fracture. | Vivek Gulati Kelechi Eseonu Junaid Sayani Nizar Ismail Chika Uzoigwe Muhammed Zaki Choudhury Pooja Gulati Adeel Aqil Saket Tibrewal | 2013 | World Journal of Orthopedics2013，4，2： | 29 |
| 3 | 急性缺血性卒中患者早期处理指南:美国心脏协会/美国卒中协会对医疗专业人员的指南显示文摘背景和目的作者提供了关于成年急性缺血性卒中患者评估和治疗方面的现有证据和处理推荐的一份概述。目标读者为涉及急性缺血性卒中患者发病48h内诊治的院前诊疗人员、医生、综合医疗保健人员和医院管理者。该指南将取代先前的2007年版指南及其2009年更新版。方法著述委员会成员由美国卒中协会卒中委员会的科学声明监督委员会指定,代表各个领域的医疗专家。在形成共识的整个过程中,严格遵循美国心脏协会的利益冲突原则。委员会成员被分配给与其专业领域相关的内容,对卒中文献进行回顾,重点为前一版指南发表之后的文献,然后按照美国心脏协会卒中委员会的证据强度分级方案起草推荐意见。结果本指南的目的是减少卒中相关的残疾和死亡。本指南支持卒中医疗体系的全局性概念以及从患者识别、急救医疗服务激活、转送和分诊到急诊室和卒中单元内最初数小时诊治的卒中诊治细节。本指南讨论了早期卒中评估和一般治疗、缺血性卒中治疗、特殊干预措施,如再灌注策略以及脑复苏的一般生理学干预原则。结论由于许多推荐意见基于有限的证据,还迫切需要对急性缺血性卒中的治疗做进一步的研究。 | Edward C. Jauch Jeffrey L. Saver Harold P. Adams Askiel Bruno J.J. (Buddy) Connors Bart M. Demaerschalk Pooja Khatri Paul W. McMullan Jr, Adnan I. Qureshi Kenneth Rosenfield Phillip A. Scott Debbie R. Summers David Z. Wang Max Wintermark Howard Yonas 李海峰(译) 岳耀先(译) 王琦(译) 张贤军(译) 丁晓君(译) | 2013 | 国际脑血管病杂志2013，21，7： | 17 |
| 4 | Implant biomaterials: A comprehensive review显示文摘Appropriate selection of the implant biomaterial is a key factor for long term success of implants. The biologic environment does not accept completely any material so to optimize biologic performance, implants should be selected to reduce the negative biologic response while maintaining adequate function. Every clinician should always gain a thorough knowledge about thedifferent biomaterials used for the dental implants. This article makes an effort to summarize various dental biomaterials which were used in the past and as well as the latest material used now. | Monika Saini Yashpal Singh Pooja Arora Vipin Arora Krati Jain | 2015 | World Journal of Clinical Cases2015，3，1： | 15 |
| 5 | MicroRNAs in lung cancer显示文摘Micro RNAs have become recognized as key players in the development of cancer. They are a family of small non-coding RNAs that can negatively regulate the expression of cancer-related genes by sequenceselective targeting of m RNAs, leading to either m RNA degradation or translational repression. Lung cancer is the leading cause of cancer-related death worldwide with a substantially low survival rate. Micro RNAs have been confirmed to play roles in lung cancer development, epithelial-mesenchymal transition and response to therapy. They are also being studied for their future use as diagnostic and prognostic biomarkers and as potential therapeutic targets. In this review we focus on the role of dysregulated micro RNA expression in lung tumorigenesis. We also discuss the role of micro RNAs in therapeutic resistance and as biomarkers. We further look into the progress made and challenges remaining in using micro RNAs for therapy in lung cancer. | Pooja Joshi Justin Middleton Young-Jun Jeon Michela Garofalo | 2014 | World Journal of Methodology2014，4，2： | 13 |
| 6 | Bile acid receptors and gastrointestinal functions显示文摘Bile acids modulate several gastrointestinal(GI)functions including electrolyte secretion and absorption,gastric emptying,and small intestinal and colonic motility.High concentrations of bile acids lead to diarrhea and are implicated in the development of esophageal,gastric and colonic cancer.Alterations in bile acid homeostasis are also implicated in the pathophysiology of irritable bowel syndrome(IBS)and inflammatory bowel disease(IBD).Our understanding of the mechanisms underlying these effects of bile acids on gut functions has been greatly enhanced by the discovery of bile acid receptors,including the nuclear receptors:farnesoid X receptor(FXR),vitamin D receptor(VDR),pregnane X receptor(PXR),and constitutive androstane receptor(CAR);and G protein-coupled receptors(GPCRs):Takeda G protein-coupled receptor 5(TGR5),sphingosine-1-phosphate receptor 2(S1PR2),and muscarinic acetylcholine receptor M3(M3R).For example,various studies provided evidence demonstrating the anti-inflammatory effects of FXR and TGR5 activation in models of intestinal inflammation.In addition,the activation of TGR5 in enteric neurons was recently shown to increase colonic motility,which may lead to bile acid-induced diarrhea(BAD).Interestingly,TGR5 induces the secretion of glucagon-like peptide-1(GLP-1)from L-cells to enhance insulin secretion and modulate glucose metabolism.Because of the importance of these receptors,agonists of TGR5 and intestine-specific FXR agonists are currently being tested as an option for the treatment of diabetes mellitus and primary bile acid diarrhea,respectively.This review summarizes current knowledge of the functional roles of bile acid receptors in the GI tract. | Alexander L.Ticho Pooja Malhotra Pradeep K.Dudeja Ravinder K.Gill Waddah A.Alrefai | 2019 | Liver Research2019，3，1： | 13 |
| 7 | Inverted internal limiting membrane flap technique for very large macular hole显示文摘AIM: To assess the anatomical and visual outcome of idiopathic macular holes greater than 1000 μm using the inverted internal limiting membrane flap technique.METHODS: This retrospective case series included 5 eyes of 5 patients with idiopathic macular hole with base diameter greater than 1000 μm who underwent inverted internal limiting membrane flap technique along with standard 23 G pars plans vitrectomy with posterior hyaloid detachment and fluid gas exchange with 12%-14% perfluoropropane(C3F8). Preoperative and postoperative visual acuity and spectral domain optical coherence tomography images were evaluated. The main outcome measures were visual outcome and macular hole closure.RESULTS: Mean age was 63.2±8.4y with all 5 subjects being females. Mean duration of symptoms was 11±14 mo with a mean postoperative follow up of 13.2 ±13 mo. The mean base diameter of the macular holes was 1420±84.8 μm(1280-1480 μm). Type 1 closure was achieved in four out of five patients, while one patient had type 2 closure using the inverted internal limiting membrane(ILM) flap technique. Median baseline BCVA was 0.79 log MAR(Snellen's equivalent 20/120) and median final BCVA0.6 log MAR(Snellen's equivalent 20/80) with mean visual improvement of approximately three lines improvement. No complications related to surgical procedure were noted.CONCLUSION: The inverted internal limiting membrane flap technique may be promising for very large macular holes with high rate of macular closure and good visual outcome. | Mitali Khodani Pooja Bansal Raja Narayanan Jay Chhablani | 2016 | International Journal of Ophthalmology(English edition)2016，9，8： | 11 |
| 8 | 绿茶儿茶酚: 对心血管疾病的防御作用(英文)显示文摘Green tea,Camellia sinensis(Theaceae),a major source of flavonoids such as catechins,has recently shown multiple cardiovascular health benefits through various experimental and clinical studies.These studies suggest that green tea catechins prevent the incidence of detrimental cardiovascular events,and also lower the cardiovascular mortality rate.Catechins present in green tea have the ability to prevent atherosclerosis,hypertension,endothelial dysfunction,ischemic heart diseases,cardiomyopathy,cardiac hypertrophy and congestive heart failure by decreasing oxidative stress,preventing inflammatory events,reducing platelet aggregation and halting the proliferation of vascular smooth muscle cells.Catechins afford an anti-oxidant effect by inducing anti-oxidant enzymes,inhibiting pro-oxidant enzymes and scavenging free radicals.Catechins present anti-inflammatory activity through the inhibition of transcriptional factor NF-κB-mediated production of cytokines and adhesion molecules.Green tea catechins interfere with vascular growth factors and thus inhibit vascular smooth muscle cell proliferation,and also inhibit thrombogenesis by suppressing platelet adhesion.Additionally,catechins could protect vascular endothelial cells and enhance vascular integrity and regulate blood pressure.In this review various experimental and clinical studies suggesting the role of green tea catechins against the markers of cardiovascular disorders and the underlying mechanisms for these actions are discussed. | Pooja Bhardwaj Deepa Khanna | 2013 | 中国天然药物2013，11，4： | 10 |
| 9 | Autochthonous halotolerant plant growth-promoting rhizobacteria promote bacoside A yield of Bacopa monnieri(L.)Nash and phytoextraction of salt-affected soil显示文摘Phytoremediation is a promising approach for reclamation of salt-affected soil.Phytoextraction is the most commonly used process,which exploits plants to absorb,immobilize,and accumulate salt in their shoots.In this study,halotolerant plant growth-promoting rhizobacteria(PGPR)were isolated from the rhizosphere of wild grasses growing naturally in salt-affected areas of Lucknow,Uttar Pradesh(India)and were tested for their efficacies of salt-tolerance and plant growth-promoting(PGP)abilities.Based on 16S rRNA sequences,the most efficient halotolerant isolates possessing PGP traits were identified as Pseudomonas plecoglossicida(KM 233646),Acinetobacter calcoaceticus(KM 233647),Bacillus flexus(KM 233648),and Bacillus safensis(KM 233652).Application of these isolates as bio-inoculants significantly(P<0.05)increased the growth and bacoside A yield of a medicinal plant,Bacopa monnieri(L.)Nash,grown on natural salt-affected soil.The phytoremediation of salt-affected soil was evident by the substantial increase in shoot Na^+:K^+ratio of bio-inoculant-treated plants.When compared to un-inoculated control plants,the soil physico-chemical properties of bio-inoculant-treated plants were improved.The shoot and root biomass(fresh and dry weights),soil enzymes,and soil nutrient parameters showed significant positive correlations with the shoot Na+:K+ratio.Consequently,the halotolerant PGPR screened in this study could be useful for the reclamation of saline soils concomitant with improved plant growth and bacoside A yield. | Umesh PANKAJ Durgesh Narain SINGH Pooja MISHRA Pooja GAUR C.S.Vivek BABU Karuna SHANKER Rajesh Kumar VERMA | 2020 | Pedosphere2020，30，5： | 9 |
| 10 | Cryotherapy in the management of premalignant and malignant conditions of the esophagus显示文摘Endoscopic cryotherapy is a relatively new thermal ablative modality used for the treatment of neoplastic lesions of the esophagus. It relies on cycles of rapid cooling and thawing to induce tissue destruction with a cryogen(liquid nitrogen or carbon dioxide) leading to intra and extra-cellular damage. Surgical treatment was once considered the standard therapeutic intervention for neoplastic diseases of the esophagus and is associated with considerable rates of morbidity and mortality. Several trials that evaluated cryotherapy in Barrett's esophagus(BE) associated neoplasia showed reasonable efficacy rates and safety profile. Cryotherapy has also found applications in the treatment of esophageal cancer, both for curative and palliative intent. Cryotherapy has also shown promising results as salvage therapy in cases refractory to radiofrequency ablation treatment. Cryoballoon focal ablation using liquid nitrogen is a novel mode of cryogen delivery which has been used for the treatment of BE with dysplasia and squamous cell carcinoma. Most common side effects of cryotherapy reported in the literature include mild chest discomfort, esophageal strictures and bleeding. In conclusion, cryotherapy is an effective and safe method for the treatment of esophageal neoplastic processes, ranging from early stages of low grade dysplasia to esophageal cancer. | Pooja Lal Prashanthi N Thota | 2018 | World Journal of Gastroenterology2018，24，43： | 5 |
| 11 | Identification, occurrence, and validation of DRE and ABRE Cis-regulatory motifs in the promoter regions of genes of Arabidopsis thaliana显示文摘Plants posses a complex co-regulatory network Researchwhich helps them to elicit a response under diverse adverse conditions.We used an in silico approach to identify the genes with both DRE and ABRE motifs in their promoter regions in Arabidopsis thaliana.Our results showed that Arabidopsis contains a set of 2,052 genes with ABRE and DRE motifs in their promoter regions.Approximately 72%or more of the total predicted 2,052 genes had a gap distance of less than 400 bp between DRE and ABRE motifs.For positional orientation of the DRE and ABRE motifs,we found that the DR form(one in direct and the other one in reverse orientation)was more prevalent than other forms.These predicted 2,052 genes include 155 transcription factors.Using microarray data from The Arabidopsis Information Resource(TAIR)database,we present 44 transcription factors out of 155 which are upregulated by more than twofold in response to osmotic stress and ABA treatment.Fifty-one transcripts from the onepredicted above were validated using semiquantitative expression analysis to support the microarray data in TAIR.Taken together,we report a set of genes containing both DRE and ABRE motifs in their promoter regions in A.thaliana,which can be useful to understand the role of ABA under osmotic stress condition. | Sonal Mishra Aparna Shukla Swati Upadhyay Sanchita Pooja Sharma Seema Singh Ujjal J.Phukan Abha Meena Feroz Khan Vineeta Tripathi Rakesh Kumar Shukla Ashok Shrama | 2014 | Journal of Integrative Plant Biology2014，56，4： | 5 |
| 12 | Management of achilles tendon injury: A current concepts systematic review显示文摘Achilles tendon rupture has been on the rise over recent years due to a variety of reasons. It is a debilitating injury with a protracted and sometimes incomplete recovery. Management strategy is a controversial topic and evidence supporting a definite approach is limited. Opinion is divided between surgical repair and conservative immobilisation in conjunction with functional orthoses. A systematic search of the literature was performed. Pubmed, Medline and EmB ase databases were searched for Achilles tendon and a variety of synonymous terms. A recent wealth of reporting suggests that conservative regimens with early weight bearing or mobilisation have equivalent or improved rates of re-rupture to operative regimes. The application of dynamic ultrasound assessment of tendon gap may prove crucial in minimising re-rupture and improving outcomes. Studies employing functional assessments have found equivalent function between operative and conservative treatments. However, no specific tests in peak power, push off strength or athletic performance have been reported and whether an advantage in operative treatment exists remains undetermined. | Vivek Gulati Matthew Jaggard Shafic Said Al-Nammari Chika Uzoigwe Pooja Gulati Nizar Ismail Charles Gibbons Chinmay Gupte | 2015 | World Journal of Orthopedics2015，6，4： | 5 |
| 13 | Strategies to mitigate the adverse effect of drought stress on crop plants-influences of soil bacteria:A review显示文摘Drought stress affects plant growth and causes significant issues in meeting global demand for food crops and fodder.Drought can cause physiological,physicochemical,and morphological changes in plants,which negatively affects plant growth and productivity.To combat this under the increasing global threat of water shortage and rapid population expansion,it is crucial to develop strategies to meet global food demands.Plant growth-promoting rhizobacteria(PGPR)may provide a safe solution to enhancing crop yields through various mechanisms.These soil bacteria can provide drought tolerance to crop plants,allowing them to survive and thrive in water-scarce conditions.Productions of phytohormones,free radical-scavenging enzymes,and stress-combating enzymes that can increase tolerance to drought-induced stress are key features of plant-associated microbial communities.This review summarizes the beneficial properties of microbes that help plants tolerate water scarcity and highlights the bacterial mechanisms that enhance drought tolerance in plants. | Priyanka CHANDRA Amoolya WUNNAVA Pooja VERMA Amaresh CHANDRA Rakesh Kumar SHARMA | 2021 | Pedosphere2021，31，3： | 4 |
| 14 | Protease inhibitors:recent advancement in its usage as a potential biocontrol agent for insect pest management显示文摘Plant derived protease inhibitors(PIs)are a promising defensin for crop im-provement and insect pest management.Although agronomist made significant efforts in utilizing PIs for managing insect pests.the potentials of PIs are still obscured.Insect ability to compensate nutrient starvation induced by dietary PI feeding using different strategies,that is,overexpression of PI-sensitive protease,expression of PI-insensitive proteases,degradation of PI,has made this innumerable collection of PIs worthless.A practical challenge for agronomist is to identify potent PI candidates,to limit insect compensatory responses and to elucidate insect compensatory and resistance mechanisms activated upon herbivory.This knowledge could be further efficiently utilized to identify potential targets for RNAi-mediated pest control.These vital genes of insects could be functionally anno-tated using the advanced gene-editing technique,CRISPR/Cas9.Contemporary research is exploiting different in silico and modern molecular biology techniques to utilize PIs in controlling insect pests efficiently.This review is structured to update recent advancements in this field,along with is chronological background. | Sujata Singh Archana Singh Sumit Kumar Pooja Mittal Indrakant K.Singh | 2020 | Insect Science2020，27，2： | 4 |
| 15 | chemistry and bioactivities of essential oils of some Ocimum species:an overview显示文摘Essential oils of different species of the genus Ocimum are natural flavouring materials of commercial importance.The data given in current literature are pertaining to the chemical composition of essential oils of different Ocimum species viz.,Ocimum basilicum Linn.(alt.Ocimum basilicum var.minimum,Ocimum basilicum var.purpurience).Ocimum campechianum Mill.,Ocimum canum Sims.(Ocimum americanum),Ocimum citriodorum,Ocimum gratissimum Linn.,Ocimum kilimandscharicum Linn..Ocimum micranthum Willd.,Ocimum sanctum Linn.,(alt.Ocimum tenuiflorum Linn.),Ocimum selloi Benth..Ocimum trichodon,Ocimum utricifolium from different geographical regions.A considerable difference in chemical composition of a particular species is found,which may be due to their occurrence in different eco-climatic zones and changes in edaphic factors.Attention is also focused on the biological properties of Ocimum oils which are related to their various interesting applications as antimicrobial,antioxidant,repellent,insecticidal,larvieidal,nematicidal and therapeutic(anti-inflammatory,antinociceptive,antipyretic,antiulcer,analgesic,anthelmintic,anticarcinogenic,skin permeation enhancer,immunomodulatory,cardio-protective,antilipidemic) agents. | Abhay Kumar Pandey Pooja Singh Nijendra Nath Tripathi | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，9： | 3 |
| 16 | 口服抗栓药预防非瓣膜性心房颤动患者卒中:美国心脏协会/美国卒中协会对医疗卫生专业人员的科学建议显示文摘成年心房颤动(atrial fibrillation,AF)患者的卒中发生率存在很大差异,其范围为每年1%~20%(平均4.5%/年),取决于合并症和既往脑血管事件病史。卒中风险分层十分重要,因为用于降低AF相关性卒中发生率的抗栓药的主要风险是出血。对于华法林而言,这涉及到对出血风险(每年1%~12%)与缺血性事件风险的权衡,它通常被用于血栓栓塞风险最高的那些患者。 | Karen L. Furie LarryB. Goldstein Gregory W. Albers Pooja Khatri Ron Neyens Mintu P. Turakhia Tanya N. Turan Kathryn A. Wood 严晓铭(译) 何晟(译) 丁妹(译) 柯开富(译) | 2012 | 国际脑血管病杂志2012，20，12： | 3 |
| 17 | Current status of pharmacological treatment of colorectal cancer显示文摘AIM: To review the clinical trials for the development in drugs for chemotherapeutic treatment of colorectal cancer(CRC).METHODS: A systematic review identified random-ized controlled trials(RCTs) assessing drugs for the treatment of CRC or adenomatous polyps from www.clinicaltrials.gov. Various online medical databases were searched for relevant publications.RESULTS: Combination treatment regimens of stan-dard drugs with newer agents have been shown to improve overall survival, disease-free survival, time to progression and quality of life compared to that with standard drugs alone in patients with advanced colorectal cancer. The FOLFOXIRI regimen has been associated with a significantly higher response rate, progression-free survival and overall survival compared to the FOLFIRI regimen. CONCLUSION: Oxaliplatin plus intravenous bolus fluo-rouracil and leucovorin has been shown to be superiorfor disease-free survival when compared to intravenous bolus fluorouracil and leucovorin. In addition, oxaliplatin regimens were more likely to result in successful surgi-cal resections. First line treatment with cetuximab plus fluorouracil, leucovorin and irinotecan has been found to reduce the risk of metastatic progression in patients with epidermal growth factor receptor-positive colorec-tal cancer with unresectable metastases. The addition of bevacizumab has been shown to significantly in-crease overall and progression-free survival when given in combination with standard therapy. | Reyhan Akhtar Shammy Chandel Pooja Sarotra Bikash Medhi | 2014 | World Journal of Gastrointestinal Oncology2014，6，6： | 3 |
| 18 | Effect of Storage Temperature on Vitamin C, Total Phenolics,UPLC Phenolic Acid Profile and Antioxidant Capacity of Eleven Potato(Solanum tuberosum) Varieties显示文摘Storage of potato tubers at low temperature affects their metabolism and may alter their phytochemical properties. There is a need to elucidate the changes in antioxidant compounds, activity and enzymes during storage of tubers. Eleven Indian potato varieties were evaluated for antioxidant parameters, after 0, 30, 60 and 90 days of storage at room temperature, 15 °C and 4 °C. Total phenolics(0.0786–0.1546 mg gallic acid equivalents·g^(-1)FW) and vitamin C content(0.0828–0.2416 mg·g^(-1)FW) varied among the varieties and were different with storage temperature; their levels fluctuated during storage but remained above the initial level until the last day of observation. Phenolic acid profiling by UPLC identified 12 compounds among which the most abundant was chlorogenic acid followed by gallic acid, sinapic acid and ellagic acid. Except para-coumaric acid which decreased at 4 °C, all the phenolic acids increased with storage. Caffeic acid, chlorogenic acid, protocatechuic acid and gallic acid mostly correlated with total phenolic content(r = 0.456, 0.482, 0.588 and 0.620, respectively). Antioxidant activity against both DPPH and ABTS radicals increased during the initial days of storage and then dropped to a level comparable or lower than the original value,irrespective of the storage temperature. Correlation study revealed that chlorogenic acid, gallic acid and ferulic acid mostly contributed to antioxidant activity. Activity of both antioxidant enzymes, superoxide dismutase and ascorbate peroxidase, increased initially but then decreased to values lower than the initial level and were not influenced by storage temperature. Correlation with antioxidant activity indicated that the enhancement of reactive oxygen scavenging species in cold stored tubers could result mainly from ascorbate peroxidase activity. Our results demonstrate that storage temperature adversely influences the metabolism and the content of antioxidant compounds in potato tubers, with subsequent increase on their antioxidant capacity. | Joseph Hubert Yamdeu Galani Pooja M.Mankad Avadh K.Shah Nilesh J.Patel Rajeshkumar R.Acharya Jayant G.Talati | 2017 | Horticultural Plant Journal2017，3，2： | 2 |
| 19 | Staphylococcus aureusβ-hemolysin-neutralizing single-domain antibody isolated from phage display library of Indian desert camel显示文摘Objective:To isolate and characterize Staphylococcus aureus(S.aureus)β-hemolysinneutralizing dAbs from phage display library of Indian desert camel.Methods:Phage display library of 5×10 dAb clones of LPS-immunized Indian desert camel constructed in our laboratory was used for selection of S.aureus exotoxin-specific clones by panning technique.Enrichment of Ag-specific clones in successive rounds of panning was assessed by phage-ELISA and phage titration.Different dAb clones binding to S.aureus exotoxin Ags were expressed with C-terminal 6×His tag in E.coli and purified by Ni-chelate chromatography.The expression was verified by SDS-PAGE and western blotting.The purified clones were tested for inhibition of ’hot-cold’ hemolytic activity in vitro.Resistance to thermal inactivation of the dAb clones was studied by observing the effect of heat treatment from 50℃to 99℃for 30 min on the ’hot-cold’ hemolytic activity in vitro.Results:Several dAb clones binding to S.aureus exotoxins were isolated and enriched by three rounds of panning.The soluble dAb clones were approximately~16 kDa in size and reacted with 6×His tag specific murine monoclonal antibody in western blot.One of the Ni-chelate affinity purified dAb.6×His clones,inhibited S.aureusβ-hemolysin activity in vitro and resisted thermal inactivation upto 991.Conclusions:An S.aureusβ-hemolysinneutralizing dAb clone of possible therapeutic potential has been isolated. | Jangra Pooja Singh Ajit | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，1： | 2 |
| 20 | Emerging industrial applications of microalgae:challenges and future perspectives显示文摘Microalgae are unicellular photosynthetic organisms that have been recently attracted potential interests and have applications in food,nutraceuticals,pharmaceuticals,animal feed,cosmetics,and biofertilizers industry.Microalgae are rich in a variety of high-value bioactive compounds which have potential benefits on human health and can be used for the prevention and curing of many disease conditions.But scale-up and safety issues remain a major challenge in the commercialization of microalgal products in a cost-effective manner.However,techniques have been developed to overcome these challenges and successfully selling the products derived from microalgae as food,cosmetics and pharmaceutical industries.Microalgae are rich in many nutrients and can be used for the production of functional food and nutraceuticals,safety and regulatory issues are major concerns and extensive research is still needed to make microalgae a commercial success in the future.Many practical difficulties are involved in making the microalgal food industry commercially viable.The present review focuses on the industrial applications of microalgae and the challenges faced during commercial production. | Aswathy Udayan Ashutosh Kumar Pandey Pooja Sharma Nidhin Sreekumar Sunil Kumar | 2021 | Systems Microbiology and Biomanufacturing2021，1，4： | 2 |
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