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| 1 | Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research. | LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER | 2008 | Chinese Science Bulletin2008，53，7： | 8 |
| 2 | Stratospheric ozone depletion and future trends of atmospheric chlorine and bromine显示文摘 | PRATHER M J WATSON R T | 1990 | Nature1990，344，： | 1 |
| 3 | Nucleartransplantation in the bovine embryo: as-sessmentof donor nuclei and recipient oocyte显示文摘 | Prather R S | 1987 | Biol Reprod1987，37，： | 1 |
| 4 | CAB reviews : perspectives in agriculture, veterinary science 显示文摘 | Ross J W Prather R S Whyte J J | 2009 | Nutr Nat Re- sour2009，4，38： | 1 |
| 5 | Strategies for activating nu- clear transfer oocytes显示文摘 | Machaty Z Prather R S | 1998 | Rep Rod Fertil Dev1998，10，78： | 1 |
| 6 | Cloning embryos by nuclear transfer显示文摘 | Prather R S Barnes F L | 1990 | Reprod Fertil1990，41，： | 1 |
| 7 | Artificial activation of porcine oocytes matured in vitro显示文摘 | PRATHER R S EICHEN P A NICKS D K | 1991 | Molecular Reproduction Development1991，28，： | 1 |
| 8 | Survey of worldwide high power wideband eapabilities显示文摘 | Prather W D Baurn C E Torres R J | 2004 | IEEE Trans on Electramagn corn- pat2004，46，3： | 1 |
| 9 | Multicentric and isolated muhifocal glioblastoma multiforme simulating metastatic disease 显示文摘 | Prather JL Long JM van Heertum R | 1976 | Br J Radiol1976，48，1： | 1 |
| 10 | Pigs is pigs显示文摘 | Prather R S | 2000 | Science2000，289，： | 1 |
| 11 | Differential effects of protein synthesis inhibitors on porcine oocyte activation显示文摘 | Nussbaum D J Prather R S | 1995 | Molecular Reproduction Development1995，41，： | 1 |
| 12 | Strategies for activation nucle- ar transfer oocytes 显示文摘 | MACHATY Z PRATHER R S | 1998 | Reproduction Fertility and Develop- ment1998，10，： | 1 |
| 13 | Effects of liquid-phase electrical conductivity, water content, and surface conductivity on the bulk soil electrical conductivity显示文摘 | RHODES J D RAATS P A C PRATHER R J | 1976 | Soil Science Society of America Journal1976，40，5： | 1 |
| 14 | Strategies for activating nuclear transfer oocytes显示文摘 | MACHATY Z PRATHER R S | 1998 | Reprod Fertil Dev1998，10，： | 1 |
| 15 | Response of porcine oocytes to electrical and chemical activation during maturation in vitro显示文摘 | HAGEN D R PRATHER R S FIRST N L | 1991 | Mol Reprod Develop1991，28，： | 1 |
| 16 | Production of cloned pigs by using somatic cells as donors显示文摘 | Lai L X Prather R S | 2003 | Cloning and Stem Cells2003，5，4： | 1 |
| 17 | Effect of LiquidPhase Electrical Conductivity,Water Content,and Surface Conductivity on Bulk Soil Electrical Conductivity显示文摘 | RHODES J RAATS P PRATHER R | | 0，，： | 1 |
| 18 | Production of cloned pigs by using somatic cells as donors显示文摘 | Lai L Prather R S | 2003 | Cloning and Stem Cells2003，5，4： | 1 |
| 19 | Microtubule and microfilament dynamics in porcine oocytes during meiotic maturation显示文摘 | Kim N H Funahashi H Prather R S | 1996 | Mol Reprod Dev1996，43，2： | 1 |
| 20 | Development of pig embryos reconstructed by microinjection of cultured fetal fibroblast cells into in vitro matured oocytes显示文摘 | Tao T Machaty Z Boquest A C Day B N Prather R S | 1999 | Anim Reprod Sci1999，56，2： | 1 |
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