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| 1 | Green fluorescent protein (GFP) transgenic pig produced by somatic cell nuclear transfer显示文摘Transgenic somatic cell nuclear transfer is a very promising route for producing transgenic farm ani-mals. Research on GFP transgenic pigs can provide useful information for breeding transgenic pigs, human disease models and human organ xenotransplantation. In this study, a liposomal transfection system was screened and transgenic embryos were reconstructed by nuclear transfer of GFP positive cells into enucleated in vitro matured oocytes. The development of reconstructed embryos both in vitro and in vivo was observed, and GFP expression was determined. The results showed that porcine fe-tal-derived fibroblast cells cultured with 4.0 μL/mL liposome and 1.6 μg/mL plasmid DNA for 6 h re-sulted in the highest transfection rate (3.6%). The percentage of GFP reconstructed embryos that de-veloped in vitro to the blastocyst stage was 10%. Of those the GFP positive percentage was 48%. Re-constructed transgenic embryos were transferred to 10 recipients. 5 of them were pregnant, and 3 de-livered 6 cloned piglets in which 4 piglets were transgenic for the GFP as verified by both GFP protein expression and GFP DNA sequence analysis. The percentage of reconstructed embryos that resulted in cloned piglets was 1.0%; while the percentage of piglets that were transgenic was 0.7%. This is the first group of transgenic cloned pigs born in China, marking a great progress in Chinese transgenic cloned pig research. | LIU ZhongHua SONG Jun WANG ZhenKun TIAN JiangTian KONG QingRan ZHENG Zhong YIN Zhi GAO Li MA HaiKun SUN Shuang LI YuTian WANG HongBin R S PRATHER | 2008 | Chinese Science Bulletin2008，53，7： | 8 |
| 2 | Optical observations of LIGO source GW 170817 by the Antarctic Survey Telescopes at Dome A,Antarctica显示文摘The LIGO detection of gravitational waves(GW) from merging black holes in 2015 marked the beginning of a new era in observational astronomy. The detection of an electromagnetic signal from a GW source is the critical next step to explore in detail the physics involved. The Antarctic Survey Telescopes(AST3),located at Dome A, Antarctica, is uniquely situated for rapid response time-domain astronomy with its continuous night-time coverage during the austral winter. We report optical observations of the GW source(GW 170817) in the nearby galaxy NGC 4993 using AST3. The data show a rapidly fading transient at around 1 day after the GW trigger, with the i-band magnitude declining from 17:23 ± 0:13 magnitude to 17:72 ± 0:09 magnitude in ~1:8 h. The brightness and time evolution of the optical transient associated with GW 170817 are broadly consistent with the predictions of models involving merging binary neutron stars. We infer from our data that the merging process ejected about ~10^(-2) solar mass of radioactive material at a speed of up to 30% the speed of light. | lei hu xuefeng wu igor andreoni michael c.b.ashley jeff cooke xiangqun cui fujia du zigao dai bozhong gu yi hu haiping lu xiaoyan li zhengyang li ensi liang liangduan liu bin ma zhaohui shang tianrui sun n.b.suntzeff charling tao syed a.uddin lifan wang xiaofeng wang haikun wen di xiao jin xu ji yang shihai yang xiangyan yuan hongyan zhou hui zhang jilin zhou zonghong zhu | 2017 | Science Bulletin2017，62，21： | 6 |
| 3 | Suppression of MAPK11 or HIPK3 reduces mutant Huntingtin levels in Huntington's disease models显示文摘 | Meng Yu Yuhua Fu Yijian Liang Haikun Song Yao Yao Peng Wu Yuwei Yao Yuyin Pan Xue Wen Lixiang Ma Saiyin Hexige Yu Ding Shouqing Luo Boxun Lu | 2017 | Cell Research2017，27，12： | 5 |
| 4 | Structure and luminescence properties of color-tunable phosphor Sr_(2)La_(3)(SiO_(4))_(3)F:Tb^(3+),Sm^(3+)显示文摘A series of single-phase and color-tunable phosphors Sr_(2)La_(3)(SiO_(4))_(3)F:0.15Tb^(3+),xSm^(3+)(SLSOF:0.15Tb^(3+),xSm^(3+)) was prepared using solid-state route.The X-ray diffraction(XRD) was used to characterize the phase of the as-prepared samples.The synthesized phosphors have apatite-type structure without other impurities.Sm^(3+) and Tb^(3+) ions substitute La^(3+) into the lattice and form a single-phase solid solution.The elements are evenly distributed in the sample according to the scanning electron microscopy(SEM) results.The crystal structure of the host phosphor was refined by the Rietveld method.The optical properties were studied in detail by investigation of the luminescence and diffuse spectra,fluorescence decay curves and energy transfer efficiency.The phosphor color can be changed from green(0.29,0.48) to yellow/orange and red(0.57,0.42) via adjusting the doping ratio of Sm^(3+).The SLSOF:0.15Tb^(3+),xSm^(3+)phosphors can emit typical multicolor light such as green,yellow,orange and red with gradually increasing Sm^(3+)doping concentration.All results indicate the occurrence of the energy transfer which results in the color-tunable properties of the phosphors. | Haikun Liu Kun Nie Yuanyuan Zhang Lefu Mei Dina V.Deyneko Xiaoxue Ma | 2023 | Journal of Rare Earths2023，41，9： | 1 |
| 5 | VMckpt:lightweight and live virtual machine checkpointing显示文摘Recent advance of virtualization technology provides a new approach to check-point/restart at the virtual machine(VM) level.In contrast to traditional process-level checkpointing,checkpointing at the virtualization layer brings up several advantages,such as compatibility,transparence,flexibility and simplicity.However,because the virtualization layer has little semantic knowledge about the operation system and the applications running atop,VM-layer checkpointing requires saving the entire operating system state rather than a single process.The overhead may render the approach impractical.To reduce the size of VM checkpoint,in this paper we propose a page eviction scheme and an incremental checkpointing mechanism to avoid saving unnecessary VM pages in the checkpoint.To keep the system online transparently,we propose a live checkpointing mechanism by saving the memory image in a copy-on-write(COW) manner.We implement the performance optimization mechanisms in a prototype system,called VMckpt.Experimental results with a group of representative applications show that our page eviction scheme and incremental checkpointing can significantly reduce the checkpoint file size by up to 87% and shorten the total checkpointing/restart time by a factor of up to 71%,in comparison with the Xens default checkpointing mechanism.The observed application downtimes due to checkpointing can be reduced to as small as 300 ms. | LIU HaiKun JIN Hai LIAO XiaoFei MA Bo XU ChengZhong | 2012 | Science China(Information Sciences)2012，55，12： | 0 |
| 6 | Earth Summit Mission 2022:Scientific Expedition and Research on Mt.Qomolangma Helps Reveal the Synergy between Westerly Winds and Monsoon and the Resulting Climatic and Environmental Effects显示文摘“Earth summit mission 2022”is one of the landmark scientific research activities of the Second Tibetan Plateau Scientific Expedition and Research(STEP).This scientific expedition firstly used advanced technology and methods to detect vertical meteorological elements and produce forecasts for mountain climbing.The“Earth summit mission 2022”Qomolangma scientific expedition exceeded an altitude of over 8000 meters for the first time and carried out a comprehensive scientific investigation mission on the summit of Mt.Qomolangma.Among the participants,the westerly–monsoon synergy and influence team stationed in the Mt.Qomolangma region had two tasks:1)detecting the vertical structure of the atmosphere for parameters such as wind,temperature,humidity,and pressure with advanced instruments for high-altitude detection at the Mt.Qomolangma base camp;and 2)observing extreme weather processes to ensure that members of the mountaineering team could successfully reach the top.Through this scientific expedition,a better understanding of the vertical structure and weather characteristics of the complex area of Mt.Qomolangma is gained. | Yaoming MA Weiqiang MA Huaguang DAI Lei ZHANG Fanglin SUN Jinqiang ZHANG Nan YAO Jianan HE Zhixuan BAI Yuejian XUAN Yunshuai ZHANG Yuan YUAN Chenyi YANG Weijun SUN Ping ZHAO Minghu DING Kongju ZHU Jie HU Bian Bazhuga Bai Juepingcuo Zhuo Ma Ren Qingnima Suo Langwangdui Yang Zong Haikun WEN | 2023 | Advances in Atmospheric Sciences2023，40，2： | 0 |
| 7 | NUIST ESM v3 Data Submission to CMIP6显示文摘This paper introduces the experimental designs and outputs of the Diagnostic,Evaluation and Characterization of Klima(DECK),historical,Scenario Model Intercomparison Project(MIP),and Paleoclimate MIP(PMIP)experiments from the Nanjing University of Information Science and Technology Earth System Model version 3(NESM3).Results show that NESM3 reasonably simulates the modern climate and the major internal modes of climate variability.In the Scenario MIP experiment,changes in the projected surface air temperature(SAT)show robust“Northern Hemisphere(NH)warmer than Southern Hemisphere(SH)”and“land warmer than ocean”patterns,as well as an El Niño-like warming over the tropical Pacific.Changes in the projected precipitation exhibit“NH wetter than SH”and“eastern hemisphere gets wetter and western hemisphere gets drier”patterns over the tropics.These precipitation patterns are driven by circulation changes owing to the inhomogeneous warming patterns.Two PMIP experiments show enlarged seasonal cycles of SAT and precipitation over the NH due to the seasonal redistribution of solar radiation.Changes in the climatological mean SAT,precipitation,and ENSO amplitudes are consistent with the results from PMIP4 models.The NESM3 outputs are available on the Earth System Grid Federation nodes for data users. | Jian CAO Libin MA Fei LIU Jing CHAI Haikun ZHAO Qiong HE Bo WANG Yan BAO Juan LI Young-min YANG Hua DENG Bin WANG | 2021 | Advances in Atmospheric Sciences2021，38，2： | 0 |
| 8 | Metal halide perovskite nanocrystals with enhanced photoluminescence and stability toward anti-counterfeiting highperformance flexible fibers显示文摘As a new type of light-collecting and luminescent material,all-inorganic cesium lead halide CsPbX_(3)(X=Cl,Br,I)perovskite nanocrystals(NCs)are expected to have a wide range of applications in the fields of photovoltaics,optoelectronics,and fluorescence anti-counterfeiting,etc.Therefore,improving the fluorescence performance and stability of CsPbX_(3)perovskite NCs to prompt their applications would promise both fundamental and practical significance for in-depth research in the field of halide perovskites.In this paper,we developed a modification strategy to introduce a halogen source,zinc bromide(ZnBr_(2))in hexane,to CsPbX_(3)perovskite that can be conducted under atmospheric conditions with reduced reaction cost and easier operation.The first work in this paper was to apply the modification strategy to CsPbI_(3)nanowires(NWs).Compared with the untreated NWs,the ZnBr_(2)/hexane modified CsPbI_(3)NWs exhibited better fluorescence properties.Subsequently,based on the study of perovskite NWs,we investigated perovskite nanocrystal-CsPbI_(3)nanorods(NRs)with different morphologies and sizes.It was found that the luminescence properties of nanorods(NRs)were superior.Later,we infiltrated the modified NRs into the aramid/polyphenylene sulfide(ACFs/PPS)composite paper yielded from our previous work to study its fluorescence performance for anti-counterfeiting.Their luminescence properties under ultraviolet light irradiation enable better performance in fluorescence anti-counterfeiting.The ZnBr_(2)/hexane modification strategy and the applications studied in this work will expand the scope of perovskite research,laying the foundation for the applications of fluorescent anti-counterfeiting,nano-photoelectric devices,and fluorescent composite materials. | Ranran Zhou Chi-An Cheng Xuyi Wang Kun Nie Jing Wu Mengyun Wu Xiuqiang Duan Ziyao Hu Injamam Ul Huq Hua Wang Luoxin Wang Lefu Mei Haikun Liu Xiaoxue Ma | 2023 | Nano Research2023，16，2： | 0 |
| 9 | Application and Assessment of Ketamine-Xylidinothiazoline Combinations for Anaesthesia in Rhesus Monkey显示文摘The quantitative anaesthesia assessment technique was used to evaluate the effectiveness of ketamine, ketamine- xylidinothiazoline in rhesus monkey. Total 20 healthy adult rhesus monkeys were divided into two groups and anaesthetized using either intramuscular (IM) ketamine (20 mg·kg-1) or ketamine (5 mg·kg-1 IM) and xylidinothiazoline (1 mg· kg-1 IM). During anaesthesia rectal temperature, respiratory rate, heart rate, saturation of blood oxygen and blood pressure were recorded. The degree of sedation, analgesia, muscle relaxation were monitored either. The results showed that ketamine alone did not produce adequate anaesthesia, and the combination of xylidinothiazoline and ketamine provided adequate anesthesia for rhesus monkeys with no signifi cant side effects and little effects on respiration and circulation. | XIAO Jianhua GAO Li Liu Huanqi FAN Honggang MA Haikun LIU Yun WANG Hongbin | 2009 | Journal of Northeast Agricultural University(English Edition)2009，16，1： | 0 |
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