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| 1 | Predominant mucosal expression of 5-HT_(4(+h)) receptor splice variants in pig stomach and colon显示文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT. | Evelien KV Priem Joris H De Maeyer Mado Vandewoestyne Dieter Deforce Romain A Lefebvre | 2013 | World Journal of Gastroenterology2013，19，24： | 2 |
| 2 | Virulence and pathogenesis of non-virulent and virulent strains of pseudorabies virus expressing envelope glycoprotein E1 of hog cholera virus显示文摘 | Mulder W A Priem J Glazenburg K L | 1994 | J Gen Virol1994，75，1： | 1 |
| 3 | Virulence and pathogenesis of non-virulent and virulent strains of pseudorabies virus expressing envelope glycoprotein E1 of hog cholera virus显示文摘 | Mulder W A Priem J Glazenburg K L | 1994 | Journal of General Virology1994，75，1： | 1 |
| 4 | Relationship between semen quality and the seminal plasma components carnitine,alpha-glucosidase,fructose,citrate and granulocyte elastase in infertile men compared with a normal population显示文摘 | Zopfgen A Priem F Sudhoff F | 2000 | Hum Reprod2000，15，4： | 1 |
| 5 | Natural perturbations, drying-wetting and freezing-thawing cycles, and the emission of nitrous oxide, carbon dioxide and methane from farmed organic soils显示文摘 | Prieme A Christensen S | 2001 | Soil Biology and Biochemistry2001，33，15： | 1 |
| 6 | Relationship between semen quality and the seminal plasma components carnitine, alpha-glucosidase, fructose, citrate and granulocyte elastase in infertile men compared with a normal population 显示文摘 | Zopfgen A Priem F Sudhoff F | 2004 | Hum Reprod2004，15，4： | 1 |
| 7 | Routine workflow for useof urine strips and urine flow cytometer UF-100 in the hospital laboratory显示文摘 | Lun A Ziebig R Priem F | 1999 | Clin Chem1999，45，81： | 1 |
| 8 | Relationship between semen quality and the seminal plasma components carnitine, alpha glucosidase, fructose, citrate and granulocyte elastase in infertile men compared with a normal population显示文摘 | Zopfgen A Priem F Sudhoff F | 2000 | Hum Reprod2000，15，4： | 1 |
| 9 | Executives' Perceptions ofUncertainty Sources: a numerical taxonomy and underlying climensions显示文摘 | Priem R L Love L G Shaffer M A | 2002 | Journal of Management2002，28，6： | 1 |
| 10 | Alderson's Transvectionand Porter's Value System:a Comparison of Two Independently-Developed Theories显示文摘 | Priem R L Rasheed A M A | 1997 | Journal of Management History1997，3，2： | 1 |
| 11 | Relationship between semen quality and the seminal plasma components carnitine,alpha-glucosidase,fructose,citrate and granulocyte elastase in infertile men compared with a normal population显示文摘 | Z(o)pfgen A Priem F Sudhoff F | 2000 | Human Reproduction2000，15，4： | 1 |
| 12 | Triclosan affects the microbial community in simulated sewage- drain-field soil and slows down xenobiotic degradation 显示文摘 | Svenningsen H Henriksen T Priem A | 2011 | Environmental Pollution2011，159，6： | 1 |
| 13 | Relationship between semen quality and the seminal plasma components carnitine , alphaglucosidase , fructose , citrate and granulocyte elastase in infertile men compared with a normal population 显示文摘 | Zopfgen A Priem F Sudhoff F | 2000 | Hum Reprod2000，15，4： | 1 |
| 14 | Experimental quantification of transmission of genetically engineered pseudorabies virus显示文摘 | Mulder W A M De Jong M C M Priem J | 1995 | Vaccine1995，13，18： | 1 |
| 15 | , Rationality in strategic decision processes, environmental dynamism and firm performance 显示文摘 | PRIEM R RASHEED A & KOTULIC A | 1995 | Journal of Management1995，，21： | 1 |
| 16 | Structured conflict and consensus outcomes in group decision making显示文摘 | Priem R L Harrison D A Muir N K | 1995 | Journal of Management1995，21，4： | 1 |
| 17 | Diversity of nitrite reductase (nirK and nirS) gene fragments in forested upland and wetland soils 显示文摘 | Prieme A Braker G Tiedje J M | 2002 | Applied and Environmental Microbiology2002，68，4： | 1 |
| 18 | Natural perturbations, drying-wetting and freezing-thawing cycles, and the emission of nitrous oxide, carbon dioxide and methane from farmed organic soils显示文摘 | Prieme A Christensen S 2001 | 2001 | Soil Biol Biochem2001，33，15： | 1 |
| 19 | Relationship between semen quality and the seminal plasma components carnitine,alpha-glucosidase,fructose,citrate and granulocyte elastase in infertile men compared with a normal population显示文摘 | Zopfgen A Priem F Sudhoff F | 2000 | Hum Reprod2000，15，： | 1 |
| 20 | Chief executive scanning emphases, environmental dynamism, and man- ufacturing firm performance 显示文摘 | Garg V K Wahers B A Priem R L | 2003 | Strategic Management Journal2003，24，： | 1 |
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