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| 1 | Caspase-3 activation as a bifurcation point between plasticity and cell death显示文摘Death-mediating proteases such as caspases and caspase-3 in particular,have been implicated in neurodegenerative processes,aging and Alzheimer's disease.However,emerging evidence suggests that in addition to their classical role in cell death,caspases play a key role in modulating synaptic function.It is remarkable that active caspases-3,which can trigger widespread damage and degeneration,aggregates in structures as delicate as synapses and persists in neurons without causing acute cell death.Here,we evaluate this dichotomy,and discuss the hypothesis that caspase-3 may be a bifurcation point in cellular signaling,able to orient the neuronal response to stress down either pathological/apoptotic pathways or towards physiological cellular remodeling.We propose that temporal,spatial and other regulators of caspase activity are key determinants of the ultimate effect of caspase-3 activation in neurons.This concept has implications for differential roles of caspase-3 activation across the lifespan.Specifically,we propose that limited caspase-3 activation is critical for synaptic function in the healthy adult brain while chronic activation is involved in degenerative processes in the aging brain. | Shikha Snigdha Erica D Smith G Aleph Prieto Carl W Cotman | 2012 | Neuroscience Bulletin2012，28，1： | 91 |
| 2 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 3 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 4 | Loss of Mda-7 expression with progression of melanoma显示文摘 | Ellerhorst J A Prieto V G Ekmekcioglu S | 2002 | J Clin Oncol2002，20，4： | 1 |
| 5 | The pharmaeokinetics and metabolism of ivermectin in domestic animal species 显示文摘 | Canga A G Prieto A M S Li6bana M J D | 2009 | The Veterinary Journal2009，179，1： | 1 |
| 6 | Accurate pedestrian indoor navigation by tightly coupling foot-mounted IMU and RFID measurements 显示文摘 | JIMENEZ R A R SECO G F PRIETO H I C | 2012 | IEEE Transactions on Instrumentation and Measurement2012，61，1： | 1 |
| 7 | Lipoxin A4 impair- ment of apoptotic signaling in macrophages: implication of the PI3 K/ Akt and the ERKlNrf - 2 defense pathways 显示文摘 | PRIETO P CUENCA J TRA YES P G et a1 | 2010 | Cell Death Differ2010，17，7： | 1 |
| 8 | Effects of Intense Pulsed Light on Sundamaged Human Skin, Routine, and Ultra Structural Analysis 显示文摘 | PRIETO V G SADICK N S LLORETA J | 2002 | Lasers Surg Med2002，30，： | 1 |
| 9 | Tanycytes present in the adult rat mediobasal hypothalamus support the regeneration of monoaminergic axons 显示文摘 | Chauvet N Prieto M Alonso G | 1998 | Exp Neurol1998，151，1： | 1 |
| 10 | Dynamics of the fragment of thrombomodulin containing tbe fourlh and fifth epidermal growth factor-like domains correlate with function 显示文摘 | Prieto JH Sampoli Benitez BA Melacini G | 2005 | Biochemistry2005，44，4： | 1 |
| 11 | Study of the interactions between lysozyme and a fully-fluorinated surfactant in aque- ous solution at different surfactant-protein ratios显示文摘 | RUSO J M GONZALE Z-P-REZ A PRIETO G | 2003 | Int J Biol Macromol2003，33，13： | 1 |
| 12 | Cell saving systems do not reduce the need of transfusion in low-risk patients undergoing cardiac surgery 显示文摘 | Reyes G Prieto M Alvarez P | 2011 | Interact Cardiovasc Thorac Surg2011，12，2： | 1 |
| 13 | Inci- dence and distribution of argininosuccinate synthetase de- ficiency in human eaneers:a method for identifying canc- ers sensitive to arginine deprivation 显示文摘 | DILLON B J PRIETO V G CURLEY S A | 2004 | Cancer2004，100，4： | 1 |
| 14 | Future therapies for hepatocellular carcinoma显示文摘 | Mazzollini G Prieto J | 2005 | Eur J Gastroenterol Hepatol2005，17，5： | 1 |
| 15 | Cross validity of the UCLA Loneliness Scale factorization显示文摘 | Borges A Prieto P Rivvhetti G | 2008 | Psicothema2008，20，4： | 1 |
| 16 | Direct electrodeposition of highly dense 50 nm Bi2Te3-ySey nanowire arrays 显示文摘 | MARISOL M G SNYDER G J PRIETO A L | 2003 | Nano Letters2003，3，： | 1 |
| 17 | Utility of adult antigens of Dirofilaria immitis for the early detection of dirofilariosis and for the evaluation of chemoprophylactic treatment in experimentally infected cats显示文摘 | PRIETO G SIMONN F GENCHI C | 1999 | Vet Parasitol1999，86，1： | 1 |
| 18 | Neural Net Approach for Blind Separation of Sources Based on Geometric Properties显示文摘 | Puntonet C G Prieto A | 1998 | Neurocomputing1998，35，5： | 1 |
| 19 | Molecular basis of xerodermapigmentosum group C DNA recognition by engineered meganucleases显示文摘 | REDONDO P PRIETO J MUNOZ I G | 2008 | Nature2008，456，： | 1 |
| 20 | Tanycytes transplanted into the adult rat spinal cord support the regeneration of lesioned axons显示文摘 | Prieto M Chauvet N Alonso G | 2000 | J Exp Neuro2000，161，1： | 1 |
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