| 1 | Synthesis and Evaluation of Novel Chitosan Derivatives for Gene Delivery显示文摘A series of novel water soluble chitosan derivatives as gene vectors was synthesized. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilizing agarose gel electrophoresis, particle size analysis, zeta potential measurement and scanning electron microscopy. Furthermore, cytotoxicity of chitosan derivatives and transfection efficiency of polyplexes were evaluated in vitro. The experimental results showed that the novel chitosan derivatives had lower cytotoxicity, good DNA condensation, and higher transfection efficiencies compared to chitosan in both 293 T and He La cell lines. It was indicated that these chitosan derivatives were promising candidates for non-viral gene vectors. | 付芳 LIU Xiong 吕波 QU Guoyi ZHENG Hua HUANG Zhijun XU Peihu XU Haixing YIN Yihua LIU Xia ZHANG Xueqiong | 2015 | Journal of Wuhan University of Technology(Materials Science)2015，30，4： | 1 |
| 2 | Efficacy and mechanisms of Dingxian pill(定痫丸)combined with valproic acid on pentylenetetrazol-induced chronic epilepsy in rats显示文摘OBJECIVE:To investigate the efficacy and mechanisms of Dingxian pill(定痫丸)combined with valproic acid(VPA)on pentylenetetrazol-induced chronical epilepsy in rats.METHODS:A rat model of epilepsy was established by administering pentylenetetrazol(PTZ)water solution(35 mg/kg).Rats were divided into 4 groups,among which three groups were treated with different drugs once a day for 28 d including Dingxian pill(2.4 g/kg),VPA(0.2 g/kg),or a combination of Dingxian pill(2.4 g/kg)and VPA(0.2 g/kg)respectively,and the control group was given the same volume of saline.Rats in different groups were compared based on animal behavior,electroencephalograms,Morris water maze,immunohistochemistry,transcriptomics and real-time polymerase chain reaction.RSULTS:The combination therapy of Dingxian pill and VPA inhibited PTZ-induced seizure-like behavior and reduced seizure grades more significantly than VPA alone.Compared with the control group,the learning and memory ability of chronic PTZ-induced epileptic rats was improved in all the drug treatment groups,especially in the group that received both Dingxian pill and VPA.Similar to the results of MWM tests,expression of the neuroexcitability marker gene c-Fos was reduced after Dingxian pill and/or VPA treatment,and the effect was most pronounced in the combined treatment group.Transcriptomic analysis revealed that gene expression in the rodent hippocampus,which is involved in epilepsy,was upregulated by combined treatment with Dingxian pill and VPA,compared with VPA treatment alone.CONCLUSION:Our results not only highlight the antiepileptic effects of combined Dingxian pill and VPA treatment,but also shed light on the underlying molecular mechanisms and provide a way to apply Traditional Chinese Medicine in the treatment of epilepsy. | QU Dongxiao GE Yiqin ZHUO Limin CHEN Liji XUE Yonghua CHENG Jiwei TAO Jie LI Guoyi ZHU Yudan XIAO Qian | 2023 | Journal of Traditional Chinese Medicine2023，43，2： | 0 |
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