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| 1 | Variation at the transcriptional level among Chinese natural populations of Arabidopsis thaliana in response to cold stress显示文摘The Arabidopsis 25K GeneChip (ATH1, Affymetrix) was used to make a survey of the variation of the transcriptional profiles among 5 Chinese natural populations of Arabidopsis thaliana under cold treatment. In normal growth condition, the expression level of 2.26% (513 genes in the population from Jiujiang, Jiangxi, JXjjx) to 6.52% (1482 genes in the population from Tongliang, Chongqing, CQtlx) genes was 2-fold higher than that of Col ecotype. Under cold treatment, the expression of 12.84% (2920 genes in the population from Chenggu, Shaanxi, SXcgx) to 19.46% (4426 genes in the population from Qinghe, Xinjiang, XJqhx) genes was up- or down-regulated by at least two-fold that of their controls. In general, most of up-regulated genes might be the genes essential for plant surviving at low temperature, such as genes in CBF pathway and the genes responsible for synthesizing molecules accumulated for cold tolerance. However, each natural population had some specific genes induced under cold treatment. The data indicated that some of the cold-responding genes were differentiated among the populations distributed in the natural habitats with different climate conditions. CBF3, one of the key tran-scription factor genes in cold responding pathway, showed significant differences in expression among populations. The sequence analysis indicated that the changes in its regulation region caused the dramatic difference in the expression pattern. Further studies on the correlation of the function of the differentially expressed genes and the cold tolerance in different populations may provide some new insight into the molecular mechanism of adaptation to local environment in Arabidopsis thaliana in China. | HE Fei KANG JiuQing ZHOU Xin SU Zhen QU LiJia GU HongYa | 2008 | Chinese Science Bulletin2008，53，19： | 9 |
| 2 | Molecular evolution of the exon 2 of CHS genes and the possibility of its application to plant phylogenetic analysis显示文摘The exon 2 of chalcone synthase (CHS) gene is relatively conserved during evolution. In this study, three exon 2 fragments from two species in gymnosperm (Cycas panzhihuaensis, Ginkgo biloba ) and seven from four species in angiosperm (Magnolia denudata, Salix babylonica, Nymphaea tetragona, Camellia japonica) have been amplified by PCR from genomic DNA and sequenced. Together with other 73 sequences of CHS collected from EMBL database and literature, these sequences, which embrace 19 families of gymnosperm and angiosperm, have been analyzed for their phylogenetic relations by parsimony method. The result indicated that sequences from the same systematic family usually grouped together except those from Theaceae, Magnoliaceae and Nymphaeaceae. The relative rate test revealed the rate heterogeneity of CHS genes among the families. For the nucleotide substitution the sequences from Asteraceae and Solanaceae evolve faster than those from the other families analyzed while the sequences from Poaceae, | WANG Jinling QU Lijia CHEN Jun GU Hongya CHEN Zhangliang | 2000 | Chinese Science Bulletin2000，45，19： | 6 |
| 3 | Methylation of phytohormones by the SABATH methyltransferases显示文摘In plants,one of the most common modifications of secondary metabolites is methylation catalyzed by various methyltransferases. Recently,a new class of methyltransferases,the SABATH family of methyltransferases,was found to modify phytohormones and other small molecules.The SABATH methyltransferases share little sequence similarity with other well characterized methyltransferases.Arabidopsis has 24 members of the SABATH methyltransferase genes,and a subset of them has been shown to catalyze the formation of methyl esters with phytohormones and other small molecules.Physiological and genetic analyses show that methylation of phytohormones plays important roles in regulating various biological processes in plants,including stress responses,leaf development,and seed maturation/germination.In this review,we focus on phytohormone methylation by the SABATH family methyltransferases and the implication of these modifications in plant development. | QU LiJia LI Shuang XING ShuFan | 2010 | Chinese Science Bulletin2010，55，21： | 6 |
| 4 | Construction of chimeric inducible promoters by elicitors of rice fungal blast pathogen and their expression in transgenic rice显示文摘The promoter fragments of wheat GstA1 and potato Gst1 have been amplified by PCR, cloned and fused respectively to the minimal promoter sequence of rice actin gene (Act1)) and its 5’ untranslated leader sequence together with GUS. The constructs with 2 chimeric promoters (WGA and PGA) have been transferred into rice in order to analyze their inducibility patterns in transgenic rice plants. The results show that: WGA and PGA are both inducible by elicitors of Pyricularia oryzae in transgenic rice cells; the intron I of rice Act1 gene is important for the heterogenic expression of monocot and dicot promoter elements in rice; and the Act1 minimal promoter and its 5’untranslated leader sequence produced low level background expression in rice. | LU Huafei MING Xiaotian QU Lijia LIU Meihua LI Jing GU Hongya CHEN Zhangliang | 2000 | Chinese Science Bulletin2000，45，3： | 5 |
| 5 | Structural and expressional analysis of a cDNA that expresses predominantly in rice stamens显示文摘The development of stamen is an important stage during the sexual reproduction in higher plants. Studying the genes that predominantly or even specifically expressed in stamens will aid in understanding the molecular mechanism of stamen development. The structure of a rice CHS-like gene (D5) which predominantly expressed in anthers has been analysized, showing high structural similarity with two other anther-specific genes (A1 and BA42) from Brassica napus. Northern analysis also reveals that the three genes share a similar expression pattern and that the expression of D5 may relate to the early development of pollens. It is concluded that D5, A1 and BA42 represent a group of diverged CHS-like genes which are related to stamen development. | Yi Zhang Lijia Qu Meihua Liu Xiaoli Qin Hongya Gu Zhangliang Chen | 1998 | Chinese Science Bulletin1998，43，9： | 2 |
| 6 | Obtaining transgenic rice resistant to rice fungal blast disease by controlled cell death strategy显示文摘The strategy of the two-component system, composed of Barnase and Barstar which encode RNase and a specific inhibitor to the RNase respectively, is adopted to obtain transgenic rice resistant to rice fungal blast disease. In this study, two chimeric promoters, induced by rice blast fungus pathogen (Magnaporthe grisea), are fused with Barnase respectively to construct two plant expression vec-tors, pWBNBS and pPBNBS together with the Barstar driven by CaMV 35S promoter. The resistance of the transgenic rice lines to rice blast fungus disease and rice blight disease are evaluated. The results show that (1) the expression of Barnase is induced in rice leaves when inoculated with the spores of Magnaporthe grisea; (2) the induced expression level of Barnase surpasses the level of Barstar, which elicits a similar hypersensitive response (HR) in the leaves, and the transgenic plant shows high resistance to the rice fungal blast disease; and (3) transgenic rice plants also show obvious re-sistance to rice bacterial blight disease. Taken together, these results suggest that the transgenic rice plants harboring this two-component system acquire relatively broad spectrum resistance against pathogens, especially high resistance to rice fungal pathogen. | MAO Shengji, GU Hongya, QU Lijia & CHEN Zhangliang National Key Laboratory of Protein Engineering and Plant Genetic En-gineering, College of Life Sciences, Peking University, Beijing 100871, China Correspondence should be addressed to Qu Lijia (e-mail: qulj@plum. lsc.pku.edu.cn) | 2003 | Chinese Science Bulletin2003，48，16： | 2 |
| 7 | An anther-specific chalcone synthase-like gene D5 related to rice pollen development显示文摘It was shown in a previous analysis that D5 gene from rice (Oryza sativa L.) was an anther-specific gene encoding a chalcone synthase-related protein. In this study, D5 gene was found specifically expressed in tapetum cells as well as in the peripheral cells of the vascular bundle of rice anthers by RNA in situ hybridization. In order to study its function, D5 was transformed into rice in both sense and antisense directions driven by a rice Actin 1 promoter. It has been observed that the pollen grains from the antisense D5 transgenic rice plants are abnormal, indicating that D5 plays a critical role in rice pollen development. | Honghong Zheng Lijia Qu Meihua Liu Yi Zhang Yunping Shen Junming Wei Naisui Pan Hongya Gu Zhangliang Chen | 2000 | Chinese Science Bulletin2000，45，21： | 2 |
| 8 | Expression and inhibitory activity analysis of a 25-kD Bowman-Birk protease inhibitor in rice显示文摘Rice Bowman-Birk inhibitors (RBBI), with one (8 kD) or two homologous domains (16 kD), were found to be effective trypsin inhibitors in vitro. In this study, we demonstrate that the 25-kD protein corresponding to the three-domain RBBI indeed ex- ists in rice in planta, and that the RBBIs are regulated by development and wounding. We also found by inhibitory activity assay that the 3:13 disulfide bond, but not the 4:5 disulfide bond, suppresses the tryp- sin-inhibitory activity, and the D3 domain of RBBI3-1 has no inhibitory activity against trypsin, chymotryp- sin, paparin or subtilisin. Mutation analyses showed that conversion from Lys to Leu or Tyr in the N-terminal P1 site in D1 domain did not create chy- motrypsin-inhibitory activity, suggesting that the structure of the reactive loop in D1 domain hinder the new inhibitory specificity at P1 site, and the chy- motrypsin-inhibitory activity might need the participa- tion of other structures, e.g. 3:13 disulfide bond. | CHEN Jun MAO Shengji XlE Yang CAO Zhongren ZHANG Yan LIU jing CHEN Zhangliang QU Lijia GU Hongya | 2006 | Chinese Science Bulletin2006，51，1： | 2 |
| 9 | A preliminary study on the origin and evolution of chalcone synthase (CHS) gene in angi- osperms显示文摘 | Huang Jinxia Qu Lijia Yahs Ji | 2004 | Acta Botanica Sinica2004，46，1： | 1 |
| 10 | An Arabidopsis Promoter Microarray and its Initial Usage in the Identification of HY5 Binding Targets in Vitro显示文摘 | Ying Gao Jinming Li Elizabeth Strickland Sujun Hua Hongyu Zhao Zhangliang Chen Lijia Qu Xing Wang Deng | 2004 | Plant Molecular Biology2004，，5： | 1 |
| 11 | A gene encoding AtPIP5K2 may be involved in regulating the sensitivity to osmotic stress显示文摘 | 宋颖琦 Yang Qian Qin Genji Qu Lijia | 2006 | High Technology Letters2006，12，3： | 0 |
| 12 | A tumor-penetrable drug nanococktail made from human histones for interventional nucleus-targeted chemophotothermal therapy of drug-resistant tumors显示文摘Nanoparticle-based chemophotothermal therapy(CPT)is a promising treatment for multidrug resistant tumors.In this study,a drug nanococktail of DIR825@histone was developed by employing doxorubicin(DOX),NIR dye IR825 and human histones for interventional nucleus-targeted CPT of multidrug resistant tumors with an interventional laser.After localized intervention,DIR825@histone penetrated tumor tissues by transcytosis,efficiently entered tumor cells and targeted the cell nuclei.DIR825@histone also exhibited good photothermal performance and thermal-triggered drug release.Efficient multidrug resistant tumor inhibition was achieved by enhanced CPT sensitization and MDR reversion via nuclear targeting.Moreover,an interventional laser assisted DIR825@histone in inhibiting multidrug resistant tumors by promoting the sufficient delivery of laser energy inside the tumor while reducing skin injury.Therefore,DIR825@histone together with this interventional nucleus-targeted CPT strategy holds great promise for treating multidrug resistant tumors. | Jianquan Guo Dongsheng Tan Chenmei Lou Shiying Guo Xing Jin Haijing Qu Lijia Jing Sijin Li | 2022 | Bioactive Materials2022，7，3： | 0 |
| 13 | Cloning and expression of a cDNA encoding ribosomal protein S4 from Rice (Oryza sativa)显示文摘A cDNA clone, pS4, has been isolated from a cDNA library prepared from rice anthers of about 1.0 mm in length. DNA sequence analysis and database search show that the cDNA encodes a protein which is highly homologous to eukaryotic 80S ribosomal protein subunit 4 (S4). Northern hybridization indicates that this gene expresses in all tissues analyzed although the expression level varies and it cannot be induced by mechanical wounding in leaves. Southern blot analysis demonstrates that this rice S4 gene is from a multigene | QU Lijia LI Donghui ZHANG Yi LIU Meihua GU Hongya CHEN Zhangliang | 2000 | Chinese Science Bulletin2000，45，2： | 0 |
| 14 | Toward a Molecular Understanding of Plant Hormone Actions显示文摘 | Chuanyou Li Jiayang Li Kang Chong Klaus Harter Youngsook Lee Jeffrey Leung Enrico Martinoia Makoto Matsuoka Remko Offringa Lijia Qu Julian Schroeder Yunde Zhao | 2016 | Molecular Plant2016，9，1： | 0 |
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