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| 1 | Expression of cyclooxygenase-2 and clinicopathologic features in human gastric adenocarcinoma显示文摘AIM: To study the expression of cyclooxygenase-2 (COX-2)gene in gastric cancer and the relationship between COX-2expression and clinicopathologic features of gastric cancer.METHODS: With reference to the expression of β-actin gene,COX-2 mRNA level was examined in cancerous tissues andadjacent noncancerous mucosa from 33 patients bysemiquantitative reverse transcription- polymerase chainreaction (RT-PCR). Quantitation of relative band Adj volumecounts was performed using molecular Analyst for windowssoftware. The COX-2 index was determined from the band Adjvolume counts ratio of COX-2 to constitutively expressed actin.RESULTS: The COX-2 index in gastric carcinoma wassignificantly higher than that in normal mucosa (0.5966±0.2659vs 0.2979+0.171, u=5.4309, P<0.01). Significantly higherexpression of COX-2 mRNA was also observed in patientswith lymph node involvement than that in those without(0.6775±0.2486 vs 0.4105±0.2182, t=2.9341, P<0.01).Furthermore, the staging in the UICC TNM classificationsignificantly correlated with COX-2 overexpression (F=3.656,P<0.05), the COX-2 index in stage Ⅲ and IV was significantlyhigher than those in stage Ⅰ and Ⅱ(q=3.2728 and q=3.4906, P<0.05). The COX-2 index showed no correlationwith patient's age, sex, blood group, tumor location, grosstyping, depth of invasion, differentiation, and the greatesttumor dimension (P>0.05).CONCLUSION: Expression of COX-2 mRNA in gastriccarcinoma was significantly higher, which may enhancelymphatic metastasis in patients with gastric carcinoma. Thestaging in the UICC TNM classification was significantlycorrelated with COX-2 over-expression. COX-2 may contributeto progression of tumor in human gastric adenocarcinoma. | Ying-WeiXue Qi-FanZhang Zhi-BingZhu QiWang Song-BinFu | 2003 | World Journal of Gastroenterology2003，9，2： | 25 |
| 2 | Fegeneration of functional alveoli by adult human SOX9^+ airway basal cell transplantation显示文摘 | Qiwang Ma Yu Ma Xiaotian Dai Tao Ren Yingjie Fu Wenbin Liu Yufei Han Yingchuan Wu Yu Cheng Ting Zhang Wei Zuo | 2018 | Protein & Cell2018，9，3： | 20 |
| 3 | Association between endogenous gene expression and growth regulation induced by TGF-β1 in human gastric cancer cells显示文摘AIM: To investigate the association between endogenous gene expression and growth regulation including proliferation and apoptosis induced by transforming growth factor-β1(TGF-β1) in human gastric cancer (GC) cells.METHODS: Reverse transcription polymerase chain reaction (RT-PCR) was performed to detect the main components of the TGF-β1/Smads signal pathway in human poorly differentiated GC cell line BGC-823. Localization of Smad proteins was also determined using immunofluorescence.Then, the BGC-823 cells were cultured in the presence or absence of TGF-β1 (10 ng/mL) for 24 and 48 h, and the effects of TGF-β1 on proliferation and apoptosis were measured by cell growth curve and flow cytometry (FCM)analysis. The ultrastructural features of BGC-823 cells with or without TGF-β1 treatment were observed under transmission electron microscope. The apoptotic cells were visualized by means of the terminal deoxynucleotidyl transferase (TdT)-mediated dTUPin situ nick end-labeling (TUNEL) method. Meanwhile, the expression levels of endogenous p15, p21 and Smad7 mRNA and the corresponding proteins in the cells were detected at 1, 2and 3 h after culture in the presence or absence of TGF-β1(10 ng/mL) by semi-quantitative RT-PCR and Western blot,respectively.RESULTS: The TGF-β1/Smad signaling was found to be intact and functional in BGC-823 cells. The growth curve revealed the most evident inhibition of cell proliferation by TGF-β1 at 48 h, and FCM assay showed G1 arrest accompanied with apoptosis induced by TGF-β1. The typical morphological changes of apoptosis were observed in cells exposed to TGF-β1. The apoptosis index (AI) in TGF-β1-treated cells was significantly higher than that in the untreated controls (10.7±1.3% vs 0.32±0.06%, P<0.01).The levels of p15, p21 and Smad7mRNA and corresponding proteins in cells were significantly up-regulated at 1 h, but gradually returned to basal levels at 3 h following TGF-β1(10 ng/mL) treatment.CONCLUSION: TGF-β1 affects both proliferation and apoptosis of GC cells through the regulation of p15 and p21,and induces transient expression of Smad 7 as a negative feedback modulation of TGF-β1 signal. Our results suggest a novel functional role of p21 as an accelerant of TGF-β1-mediated apoptosis in GC cells. | XueLi Yun-YanZhang Qiwang Song-BinFu | 2005 | World Journal of Gastroenterology2005，11，1： | 12 |
| 4 | Apoptosis of human gastric adenocarcinoma cells induced by β-ionone显示文摘AIM:To investigate the effect of β-ionone on the growth and apoptosis of gastric adenocarcinoma cell line SGC-7901.METHODS: Using M-IT, fluorescence dye (Hoechst-33258),transmission electron microscopy and the TUNEL assay,we examined growth and apoptosis of SGC-7901 cells treated with β-ionone at various concentrations (i.e. 25, 50, 100 and 200μmol/L) for 24h,48h.RESULTS:The growth of SGC-7901 cells was inhibited by β-ionone. Seven days after treatment with β-ionone at four concentrations, the inhibition rates were 12.04%, 30.59%,78.25% and 94.15%, respectively. The IC50 value of β-ionone for SGC-7901 cells was estimated to be 89μmol/L.The apoptotic morphology was demonstrated in SGC-7901 cells treated with β-ionone by Hoechst-33258 staining and electron microscopy. Apoptosis was also shown in β-iononetreated SGC-7901 cells by the TUNEL assay.CONCLUSION:β-ionone can inhibit cell proliferation and induce apoptosis of SGC-7901 cells.However, the mechanism needs to be further investigated. | Jia-RenLiu 3ing-QingChen Bao-FengYang Hong-WeiDong Chang-HaoSun Qiwang GuoSong You-Qiangsong | 2004 | World Journal of Gastroenterology2004，10，3： | 6 |
| 5 | Effects of cg,t11-conjugated linoleic acid on adhesion of human gastric carcinoma cell line SGC-7901显示文摘AIM: To investigate the effect of c9, t11-conjugated linoleic acid (c9,t11-CLA) on the adhesion of human gastric carcinoma cell line (SGC-7901).METHODS: SGC-7901 cells were at first treated with different concentrations (25, 50, 100, 200 μmol/L) of c9,t111-CLA and 1 mL/L ethanol (as a negative control) for 24 h.Using adhesion assay and Western blot, we investigated the ability of SGC-7901 cells to adhere to intracellular matrix and examined the expression of E-cadherin (ECD), α-catenin,intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in these cells.RESULTS: The attachment rate to laminin of SGC-7901 cells treated with different concentrations of c9,t11-CLA (0,25, 50, 100, and 200 μmol/L) was 100.0±3.3, 95.7±4.0,89.2±4.6, 87.9±6.1, and 65.9±5.8, respectively. The attachment rate to fibronectin was 100.0±4.7, 96.8±3.8,94.5±4.1, 76.5±4.3, and 61.8±4.8, respectively. The attachment rate to Matrigel was 99.9±6.6, 91.4±6.8,85.5±7.4, 79.3±5.6, and 69.6±5.1, respectively. Besides,c9,t11-CLA could increase the level of ECD and α-catenin,and decrease the level of ICAM-1 and VCAM-1 in SGC-7901 cells.CONCLUSION: c9, t11-CLA can reduce the adhesion of human gastric carcinoma cells to laminin, fibronectin and Matrigel. c9,t11-CLA can increase the level of ECD and α-catenin, and decrease the level of ICAM-1 and VCAM-1 in human gastric carcinoma cells. | Bing-QingChen Yan-MeiYang QiWang Yan-HuiGao Jia-RenLiu Jing-ShuZhang Xuan-LinWang Rui-HaiLiu | 2004 | World Journal of Gastroenterology2004，10，10： | 3 |
| 6 | Identification of differential genes in the ovary relative to the testis and their expression patterns in half-smooth tongue sole (Cynoglossus semilaevis)显示文摘Half-smooth tongue sole (Cynoglossus semilaevis) is a rare marine flatfish whose mature ovary and testis greatly differ in volume and weight. The length and weight of mature females are over twice greater than those of mature males. To obtain sufficient information on gonad differentiation and the relationship between gonad development and growth in the fish, we compared gene expression between the ovary and testis using suppression subtractive hybridization (SSH). Testis cDNAs are subtracted from ovary cDNAs and are used to es- tablish an ovary testis-subtracted cDNA library. A total of 41 nonredundant clones are identified, including 20 known cDNAs, 9 unchar-acterized cDNAs (EST clones), and 12 novel sequences. For selected genes such as ZPC, RacGAP, survivin, aquaporin, CPEB, O5, O15, and O18, gene expression patterns are analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR). The results confirm that these genes are only expressed in the ovary and not in the testis, or at higher levels in the ovary than in the testis. At the same time, expressions of certain genes such as ZPC, survivin, aquaporin, CPEB, and O15 are demonstrated to possess sexual dimorphism in the kidney or muscle, and spleen. The results suggest that these genes could play key roles not only in the ovary but in other female tissues as well. | Yeying Sun Quanqi Zhang Jie Qi Yanjie Chen Qiwang Zhong Chunmei Li | 2010 | Journal of Genetics and Genomics2010，37，2： | 3 |
| 7 | Elevated-temperature properties of one long-life high-strength gun steel显示文摘The hardness, tensile strength and impact toughness of one quenched and tempered steel with nominal composition of Fe0.25C-3.0Cr-3.0Mo-0.6Ni-0.1Nb (mass fraction) both at room temperature and at elevated temperatures were investigated in order to develop high-strength steel for long-life gun barrel use. It is found that the steel has lower decrease rate of tensile strength at elevated temperature in comparison with the commonly used G4335V high-strength gun steel, which contains higher Ni and lower Cr and Mo contents. The high elevated-temperature strength of the steel is attributed to the strong secondary hardening effect and high tempering softening resistance caused by the tempering precipitation of fine Mo-rich M2C carbides in the α-Fe matrix. The experimental steel is not susceptible to secondary hardening embrittlement, meanwhile, its room-temperature impact energy is much higher than the normal requirement of impact toughness for high strength gun steels. Therefore, the steel is suitable for production of long-life high-strength gun barrels with the combination of superior elevated-temperature strength and good impact toughness. | MaoqiuWang HanDong QiWang | 2004 | Journal of University of Science and Technology Beijing2004，11，1： | 3 |
| 8 | Enhancement of oral bioavailability and anti-Parkinsonian efficacy of resveratrol through a nanocrystal formulation显示文摘Resveratrol(RES),a non-flavonoid polyphenol extracted from a wide variety of plants,exhibits neuroprotective activities against Parkinson’s disease(PD).However,undesirable water solubility of RES reduces its oral bioavailability and demonstrates lowefficacy in blood and brain,thus limiting its application.In present study,a nanocrystal formulation of RES(RES-NCs)was developed to enhance its oral bioavailability and delivery into brain for PD treatment.RES-NCs were fabricated with hydroxypropyl methylcellulose(HPMC)stabilizer via antisolvent precipitation approach.The obtained RES-NCs displayed the particle size of 222.54±1.66 nm,the PDI of 0.125±0.035,the zeta potential of−9.41±0.37mV,and a rapid in vitro dissolution rate.Molecular dynamics simulation of RES and HPMC revealed an interaction energy of−68.09 kJ/mol and a binding energy of−30.98±0.388 kJ/mol,indicating that the spontaneous binding between the two molecules is through van der Waals forces.RES-NCs conferred enhanced cellular uptake as well as improved permeability relative to pure RES.In addition,RES-NCs were able to protect neurons against cytotoxicity induced by MPP+.Meanwhile,RES-NCs exerted no significant toxic effects on zebrafish embryos and larvae,and did not influence their survival and hatching rates.When orally administered to rats,RES-NCs exhibited more favorable pharmacokinetics than pure RES,with higher plasma and brain concentrations.More importantly,MPTP-induced PD mice showed notable improvements in behavior,attenuated dopamine deficiency,and elevated levels of dopamine and its metabolites after the treatment with RES-NCs.Furthermore,immunoblot analysis revealed that the neuroprotective role of RES-NCsmay be at least partially mediated by Akt/Gsk3βsignaling pathway.Taken altogether,RES-NCs can serve as a potential treatment modality for PD,offering means of improving RES oral bioavailability and brain accumulation. | Sha Xiong Wei Liu Yile Zhou Yousheng Mo Yao Liu Xiaojia Chen Huafeng Pan Dongsheng Yuan QiWang Tongkai Chen | 2020 | Asian Journal of Pharmaceutical Sciences2020，15，4： | 3 |
| 9 | A New Efficient Synthesis of p-Nitrocalix[4]arene显示文摘A new efficient synthesis of p-nitrocalix[4]arene from calix[4]arene by using nitrogen dioxide is described.The compound is an useful intermediates for the introduction of other functional groups to obtain N containing substituted calix[4]arene.The reaction mechanism is briefly discussed. | YongLI JiaSongWANG QiWANG DeShanLI | 2004 | Chinese Chemical Letters2004，15，4： | 2 |
| 10 | Purification and characterization of hatching enzyme from brine shrimp Artemia salina显示文摘由把 Artemia 卵壳用作特定的底层,从 Artemia 盐咸滩(AHE ) 的孵化的酶被胶化过滤和离子交换层析净化,并且在这研究生物化学地并且酶地描绘了。AHE 由钠 dodecyl sulfatepolyacrylamide 胶化电气泳动有 82.2 kDa 的分子的重量,这被发现,并且经常在准备包含了 73.3 个 kDa 分子。AHE 举办了明显的 choriolytic 活动,哪个在 pH 是最佳的 7.0 并且 40 的温度 ?? 琠慲獮畤瑣潩 ???????????????????? ??????? ?? 僚吗?? | Tingjun Fan Jing Wang Wenpeng Yuan Qiwang Zhong Ying Shi Rishan Cong | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 2 |
| 11 | GST polymorphisms are associated with hepatocellular carcinoma risk in Chinese population显示文摘AIM: To investigate the association between GSTM1 and GSTT1 polymorphisms and the risk of hepatocellular carcinoma (HCC) in Chinese population. METHODS: Literature databases including PubMed, ISI web of science and other databases were searched.Pooled odds ratio (OR) and 95% CI were calculated using random- or fixed-effects model. Subgroup analysis and sensitivity analysis were also performed. RESULTS: Nineteen studies of GSTM1 (2660 cases and 4017 controls) and 16 studies of GSTT1 (2410 cases and 3669 controls) were included. The GSTM1/GSTT1 null genotypes were associated with increased risk of HCC in Chinese population (for GSTM1, OR = 1.487, 95% CI: 1.159 to 1.908, P = 0.002; for GSTT1, OR = 1.510, 95% CI: 1.236 to 1.845, P = 0.000). No publication bias was detected. In subgroup analysis, glutathione S-transferases polymorphisms were significantly associated with HCC risk among the subjects living in high-incidence areas, but not among the subjects living in low-incidence areas. CONCLUSION: The present meta-analysis suggests that GSTM1/GSTT1 null genotypes are associated with increased risk of HCC in Chinese population. | LeiYu Chun-YuWang BoXi LeiSun Ruo。Qiwang Yin—KunYan Li-YingZhu | 2011 | World Journal of Gastroenterology2011，17，27： | 2 |
| 12 | The wireless sensor network localization algorithm based on improved pattern genetic algorithm 显示文摘 | HUANG Haiping CAI Qiwang WANG Ruchuan | 2011 | Jour- nal of Computational Information Systems2011，7，9： | 1 |
| 13 | Perfluorohexane‐Encapsulated Mesoporous Silica Nanocapsules as Enhancement Agents for Highly Efficient High Intensity Focused Ultrasound (HIFU)显示文摘 | XiaWang HangrongChen YuChen MingMa KunZhang FaqiLi YuanyiZheng DepingZeng QiWang JianlinShi | 2012 | Adv Mater2012，，6： | 1 |
| 14 | N6-methyladenosine-modified DBT alleviates lipid accumulation and inhibits tumor progression in clear cell renal cell carcinoma through the ANXA2/YAP axis-regulated Hippo pathway显示文摘Background:The mechanism of metabolism reprogramming is an unsolved problem in clear cell renal cell carcinoma(ccRCC).Recently,it was discovered that the Hippo pathway altered tumor metabolism and promoted tumor progression.Thus,this study aimed at identifying key regulators of metabolism reprogramming and the Hippo pathway in ccRCC and pinpointing potential therapeutic targets for ccRCC patients.Methods:Hippo-related gene sets and metabolic gene sets were used to screen potential regulators of the Hippo pathway in ccRCC.Public databases and samples from patients were applied to investigate the association of dihydrolipoamide branched chain transacylase E2(DBT)with ccRCC and Hippo signaling.The role of DBT was confirmed by gain or loss of function assays in vitro and in vivo.Mechanistic results were yielded by luciferase reporter assay,immunoprecipitation,mass spectroscopy,and mutational studies.Results:DBT was confirmed as a Hippo-related marker with significant prognostic predictive value,and its downregulationwas caused bymethyltransferaselike-3(METTL3)-mediated N6-methyladenosine(m6A)modification in ccRCC.Functional studies specified DBT as a tumor suppressor for inhibiting tumor progression and correcting the lipid metabolism disorder in ccRCC.Mechanistic findings revealed that annexin A2(ANXA2)interacted with the lipoyl-binding domain of DBT to activate Hippo signaling which led to decreased nuclear localization of yes1-associated transcriptional regulator(YAP)and transcriptional repression of lipogenic genes.Conclusions:This study demonstrated a tumor-suppressive role for the DBT/ANXA2/YAP axis-regulated Hippo signaling and suggested DBT as a potential target for pharmaceutical intervention in ccRCC. | Daojia Miao QiWang Jian Shi Qingyang Lv Diaoyi Tan Chuanyi Zhao Zhiyong Xiong Xiaoping Zhang | 2023 | Cancer Communications2023，43，4： | 1 |
| 15 | Full-length sequence and expression analysis of a myeloid differentiation factor88(MyD88)in half-smooth tongue sole Cynoglossus semilaevis显示文摘 | Yan Yu Qiwang Zhong Quanqi Zhang | 2009 | International Journal of Immunogenetics2009，36，3： | 1 |
| 16 | Transient cooling of electronics using phase change material(PCM) -based heat sinks显示文摘 | RAVI K XIANG QIWANG ARUN S M | 2008 | J Applied Thermal Engineering2008，28，： | 1 |
| 17 | Perfluorohexane‐Encapsulated Mesoporous Silica Nanocapsules as Enhancement Agents for Highly Efficient High Intensity Focused Ultrasound (HIFU)显示文摘 | XiaWang HangrongChen YuChen MingMa KunZhang FaqiLi YuanyiZheng DepingZeng QiWang JianlinShi | 2012 | Adv. Mater2012，，： | 1 |
| 18 | Studies on the method and mechanism of FGD by liquid biochemical ways显示文摘 | Liu Qiwang Junjiao Zhang | 2002 | Acta Scientiarum Naturalium Universitatis NeiMongol2002，33，5： | 1 |
| 19 | c-fos: A key regulator of osteoclast - macrophage lineage determination and bone remodeling显示文摘 | Agamemnon E Grigoriadis Zhao qiwang | 1994 | Science1994，266，： | 1 |
| 20 | Efficient implementation of weighted ENO sehemes显示文摘 | Jiang Guangshan Shu Qiwang | 1996 | Journal of Computational Physics1996，126，1： | 1 |
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