|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells显示文摘AIM: To evaluate the effects of sulindac in inducing growth inhibition and apoptosis of human gastric cancer cells in comparison with human hepatocellular carcinoma (HCC)cells.METHODS: The human gastric cancer cell lines MKN45 and MKN28 and human hepatocellular carcinoma cell lines HepG2and SMMC7721 were used for the study. Anti-proliferative effect was measured by MTT assay, and apoptosis was determined by Hoechst-33258 staining, electronography and DNA fragmentation. The protein of cyclooxygenase-2 (COX(2) and Bcl-2 were detected by Westem dot blotting.RESULTS: Sulindac could initiate growth inhibition and apoptosis of MKN45, MKN28, HepG2 and SMMC7721 cells in a dose-and time-dependent manner. Growth inhibitory activity and apoptosis were more sensitive in HepG2 cells than in SMMC7721 cells, MKN45 and MKN28 cells. After 24hours incubation with sulindac at 2mmol. L-1 and 4mmol.L-1, the level of COX-2 and Bcl-2 protein were lowered in MKN45, SMMC7721 and HepG2 cells but not in MKN28 cells.CONCLUSION: Sulindac could inhibit the growth of gastric cancer cells and HCC cells effectively in vitro by apoptosis induction, which was associated with regression of COX-2and Bcl-2 expression. The growth inhibition and apoptosis of HCC cells were greater then that of human gastric cancer cells. The different effects of apoptosis in gastric cancer cells may be related to the differentiation of the cells. | Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，6： | 64 |
| 2 | Induction of apoptosis by arsenic trioxide and hydroxycamptothecin in gastric cancer cells in vitro显示文摘AIM To study the effects of arsenic trioxide andHCPT on different degrees of differentiated gastriccancer cells(SGC-7901,MKN-45,MKN-28)withrespect to both cytotoxicity and induction ofapoptosis in vitro.METHODS The cytotoxicity of As2O3 and HCPTon gastric cancer cells was determined by MTTassay.Morphologic changes of apoptosis ofgastric cancer cells were observed by lightmicroscopy and transmission electron microscopy.Apoptosis and cell cycle changes of gastric cancercells induced by HCPT and As2O3 were investigatedby TUNEL method and flow cytometry.RESULTS As2O3 and HCPT had remarkablecytotoxic effects on different degrees ofdifferentiated gastric cancer cells.The IC50ofAs2O3 on well differentiated gastric cancer cellMKN-28,moderately differentiated gastric cancercell SGC-7901,and poorly differentiated gastriccancer cell MKN-28 were 8.91 μmol/L,10.57μmol/L,and 11.65 μmol/L,respectively.The IC50of HCPT on MKN-28,SGC-7901,and MKN-45 were9.35 mg/L,10.21 mg/L,and 12.63 mg/Lrespectively after 48 h treatment.After 12 h ofexposure to both drugs,gastric cancer cellsexhibited morphologic features of apoptosis,including cell shrinkage,nuclear condensation, and formation of apoptotic bodies.A typicalsubdiploid peak before G0/G1 phase was observedby flow cytometry.The apoptotic rates of SGC-7901,MKN-45,and MKN-28 were 13.84%,22.52%,and 9.68%,respectively after 48 hexposure to 10 μmol/L As2O3.The apoptotic ratesof SGC-7901,MKN-45,and MKN-28 were 21.88%,12.35%,and 30.26%,respectively after 48 hexposure to 10 mg/L HCPT.The apoptotic indicewere 7%-15% as assessed by TUNEL method.The effect of As2O3 on SGC-7901 showedremarkable cell cycle specificity,which inducedcell death in G1 phase,and blocked G2/M phase.HCPT also showed a remarkable cell cyclespecificity,by inducing cell death and apoptosis inG1 phase and arrest of proliferation at S phase.CONCLUSION As2O3 and HCPT exhibitsignificant cytotoxicity on gastric cancer cells byinduction of apoptosis.As2O3 and HCPT mighthave a promising prospect in the treatment ofgastric cancer,which needs to be further studied. | Tu SP Zhong J Tan JH Jiang XH Qiao MM Wu YX Jiang SH | 2000 | World Journal of Gastroenterology2000，6，4： | 43 |
| 3 | Multicenter case-control study of the risk factors for ulcerative colitis in China显示文摘AIM:To evaluate potential risk factors in the development of ulcerative colitis(UC) in China.METHODS:A total of 1308 patients with UC and 1308 age-matched and sex-matched controls were prospectively studied in China.The UC cases were collected from 17 hospitals in China from April 2007 to April 2010.Uniform questionnaires were designed to investigate risk factors including smoking,appendectomy,stress,socio-economic conditions,nonsteroidal antiinflammatory drugs(NSAIDs),oral contraceptives,diet,breastfeeding,infections and family sanitary conditions.Group comparisons by each factor were done using simple logistic regression analysis.Conditional logistic regression was used for multivariate analysis.RESULTS:By univariate analysis,the variables predictive of UC included feeling stress,light and heavy alcoholic drinking,spicy food,sugar consumption and infectious diarrhea,while heavy tea intake and tap water consumption were protective against UC.On multivariate analysis,the protective factor for UC was tap water consumption [odds ratios(OR) = 0.424,95%CI:0.302-0.594,P < 0.001];while the potential risk factors for UC were heavy sugar consumption(OR = 1.632,95%CI:1.156-2.305,P < 0.001),spicy food(light intake:OR = 3.329,95%CI:2.282-4.857,P < 0.001;heavy intake:OR = 3.979,95%CI:2.700-5.863,P < 0.001),and often feeling stress(OR = 1.981,95%CI:1.447-2.711,P < 0.001).Other factors,such as smoking habit,appendectomy,breastfeeding,a history of measles,rural or urban residence,education,oral contraceptives,and NSAID use have not been found to have a significant association with the development of UC in the present study.CONCLUSION:Our study showed tap water consumption was a protective factor for UC,while spicy food,heavy sugar consumption and often feeling stress were risk factors for UC in this Chinese population. | Yu-Fang Wang Qin Ou-yang Bing Xia Li-Na Liu Fang Gu Kai-Fang Zhou Qiao Mei Rui-Hua Shi Zhi-Hua Ran Xiao-Di Wang Pin-Jin Hu Kai-Chun Wu Xin-Guang Liu Ying-Lei Miao Ying Han Xiao-Ping Wu Guo-Bing He Jie Zhong Guan-Jian Liu | 2013 | World Journal of Gastroenterology2013，19，11： | 24 |
| 4 | Protective effects of polydatin against CCl_4-induced injury to primarily cultured rat hepatocytes显示文摘NTRODUCTIONPolygonumcuspidatumSieb.etZucc.(Polygonaceae)isatraditionalChineseherbaldrug,withbitertasteandcoldnature.Itmainly... | HUANG Zhao Sheng, WANG Zong Wei, LIU Ming Ping, ZHONG Shi Qing, LI Qiao Mei and RONG Xiang Lu | 1999 | World Journal of Gastroenterology1999，5，1： | 24 |
| 5 | Graphene oxide as a water transporter promoting germination of plants in soil显示文摘Graphene 氧化物(去) 是生纳入基础飞机并且到 graphene 格子的边的各种各样的包含氧的功能的组的 graphene 衍生物,因此,众多的恐水病的 sp 2 在聚类的唯一的结构在吸水的 sp 3 公司矩阵以内被孤立。在这研究,吸水的性质和搬运水的性质去被利用支持工厂的萌芽和生长。它被发现那低剂量变显著地在土壤支持了菠菜和细香葱的萌芽。包含氧的功能的组变镇定水,和搬运的恐水病的 sp 2 领域流水到种子加速植物的萌芽。在之间的强壮的相互作用去,稳定的土壤谷物的表面在土壤和阻止的驱散去去。另外,不去在表面上或在工厂的房间内被检测;这发现证实了那去不是 phytotoxic。因此,去可以用作有希望的无毒的添加剂增加工厂收益。 | Yijia He Ruirui Hu Yujia Zhong Xuanliang Zhao Qiao Chen Hongwei Zhu | 2018 | Nano Research2018，11，4： | 20 |
| 6 | Surgical strategies for glioma involving language areas显示文摘gliomas 在的背景成功的处理或邻近语言区域组成主要挑战到神经外科。现在的学习被执行评估经由 intraoperative 印射直接外皮的电的刺激在下面的语言的过程当表现在 resective glioma surgery.Methods 以前时,唤醒麻醉有 gliomas 和左半球的优势的三十个病人并且,经历了经由 intraoperative 印射直接外皮的电的刺激在下面的语言在 resective glioma 外科前唤醒麻醉,回顾地被分析。所有病人有肿瘤在或邻近外皮的语言区域。大脑损害与语言功能的区域的保藏根据解剖功能的边界被移开。外科手术前、手术后的功能的调查结果是 evaluated.Results Intraoperative 语言区域在 20 个病人然而并非在四个病人被检测。为对麻醉或到 intracranial 压力的 intraoperative 增加可归因的原因印射失败的语言在在六月后续以后与中等或严重的语言赤字介绍的六个 cases.Seven 病人发生了。全部的切除术在四 cases.Conclusions Intraoperative 外皮的电的刺激在 14 种情况,在 12 种情况中的近总数的切除术和小计切除术中被完成是语言外皮的鉴定的一条精确、安全的途径。唤醒颅骨切开术 intraoperative 外皮的电的刺激,在有 presurgical 的联合识别肿瘤切除术的解剖功能的边界的神经病学的功能的成像,当保存正常语言功能并且最小化手术后的语言赤字的风险时,允许广泛的肿瘤切除。 | ZHANG Zhong JIANG Tao XIE Jian LIU Fu-sheng LI Shou-wei QIAO Hui WANG Zhong-cheng | 2008 | Chinese Medical Journal2008，，18： | 18 |
| 7 | Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis显示文摘人的大脑开发的细胞的复杂性强烈地被调查了,尽管全部人的服的外皮的地区性的描述没基于单个房间的 transcriptome 分析被报导。这里,我们从人的中间怀孕期的胚胎的 22 个大脑区域在超过 4,000 个单个房间上执行了 RNA-seq。我们识别了 29 房间亚簇,它证明在每个区域和脑桥的不同比例显示出星形细胞的特别高的百分比。胚胎的神经原不象成年神经原一样多样,尽管他们在成年人拥有了他们的命运的重要特征。神经原开发是在服的外皮的 unsynchronized,当背面的区域看起来比在这个阶段的腹的区域更成熟。区域特定的基因包括地在每 neuronal 亚簇被识别,并且这些基因的一个大比例是联系的神经疾病。我们的结果介绍区域化的基因表示的一处系统的风景和人的服的外皮的神经原成熟。 | Xiaoying Fan Ji Dong Suijuan Zhong Yuan Wei Qian Wu Liying Yan Jun Yong Le Sun Xiaoye Wang Yangyu Zhao Wei Wang Jie Yan Xiaoqun Wang Jie Qiao Fuchou Tang | 2018 | Cell Research2018，28，7： | 11 |
| 8 | IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity显示文摘Obesity and associated metabolic diseases are characterized by a chronic low-grade inflammatory state with the infiltration of many inflammatory cells,especially macrophages.Immune molecules,including some cytokines,have a close relationship with metabolism.Interleukin(IL)-25 is a member of the IL-17 cytokine family that can regulate macrophages and alleviate some metabolic dysfunction;however,its role and mechanisms in lipid metabolism remain to be extensively clarified.Human serum and liver biopsy specimens,high-fat diet-induced obesity mice and DB/DB(Lepr−/−)animal models were used to examine IL-25 expression in obesity and nonalcoholic fatty liver diseases(NAFLD).To observe the role of IL-25 in lipid metabolism,model mice were administered with IL-25 or adoptively transferred with IL-25-educated macrophages in vivo,whereas bone marrow-derived macrophages,the macrophage cell line RAW264.7 and adipocytes differentiated from 3T3-L1 were used in vitro.IL-25 was decreased in NAFLD patients and obese mice.In addition,IL-25 reduced body weight gain and lipid accumulation,enhanced lipid uptake by macrophages and increased the expression of lipolysis andβ-oxidation enzymes via alternatively activating macrophages.IL-25 also promoted lipolysis and suppressed lipogenesis in adipocytes co-cultured with the IL-25-educated macrophages.Furthermore,IL-25 improved the mitochondrial respiratory capacity and oxygen consumption rate of macrophages and produced more NAD+/NADH and ATP.In conclusion,IL-25 can stimulate M2 macrophage polarization and thereby promote lipolysis and mitochondrial respiratory capacity,highlighting the potential for IL-25 to be used as a therapeutic agent against obesity and associated metabolic syndromes. | Juan Feng Lingyi Li Zhiying Ou Qiao Li Baoyong Gong Zhenxian Zhao Weiwei Qi Ti Zhou Jun Zhong Weibin Cai Xia Yang Aiping Zhao Guoquan Gao Zhonghan Yang | 2018 | Cellular & Molecular Immunology2018，15，5： | 10 |
| 9 | Type Ⅳ secretion system in Helicobacter pylori:a new insight into pathogenicity显示文摘在数据在这评论使用了的 Helicobacter pylori.Data 来源在类型 IV 分泌物系统( T4SS )上考察研究进步的目的被在网上 PUBMED ( 1995-2007 )寻找识别这的主要先驱调查者写的主要原来的文章和批评评论回答的资源学习选择是 selected.Results 研究进步在在 Helicobacter pylori 的 T4SS 上是 summarized.The 结构,函数是 discussed.Conclusions T4SS 不仅在毒素 secretio | ZHONG Qiao SHAO Shi-he CUI Lei-lei MU Run-hong JU Xiao-li DONG Su-rong | 2007 | Chinese Medical Journal2007，，23： | 9 |
| 10 | Successful repair of acute type A aortic dissection during pregnancy at 16th gestational week with maternal and fetal survival: A case report and review of the literature显示文摘BACKGROUND Aortic dissection during pregnancy is a rare but life-threatening event for mothers and fetuses. It often occurs in the third trimester of pregnancy and the postpartum period. Most patients have connective tissue diseases such as Marfan syndrome. Thus, the successful repair of a sporadic aortic dissection with maternal and fetal survival in the early second trimester is extremely rare. CASE SUMMARY A 28-year-old woman without Marfan syndrome presented with chest pain at the 16th gestational week. Aortic computed tomographic angiography confirmed an acute type A aortic dissection (TAAD) with aortic arch and descending aorta involvement. Preoperative fetal ultrasound confirmed that the fetus was stable in the uterus. The patient underwent total arch replacement with a frozen elephant trunk using moderate hypothermic circulatory arrest with the fetus in situ. The patient recovered uneventfully and continued to be pregnant after discharge. At the 38^th gestational week, she delivered a healthy female infant by cesarean section. After 2.5 years of follow-up, the patient is uneventful and the child’s development is normal. CONCLUSION A fetus in the second trimester may have a high possibility of survival and healthy growth after aortic arch surgery. | Su-Wei Chen Yong-Liang Zhong Yi-Peng Ge Zhi-Yu Qiao Cheng-Nan Li Jun-Ming Zhu Li-Zhong Sun | 2019 | World Journal of Clinical Cases2019，7，18： | 9 |
| 11 | APPLICATION OF GENETIC DEAFNESS GENE CHIP FOR DETECTION OF GENE MUTATION OF DEAFNESS IN PREGNANT WOMEN显示文摘Objective The study is to identify the carrier rate of common deafness mutation in Chinese pregnant women via detecting deafness gene mutations with gene chip.Methods The pregnant women in obstetric clinic without hearing impairment and hearing disorders family history were selected.The informed consent was signed.Peripheral blood was taken to extract genomic DNA.Application of genetic deafness gene chip for detecting 9 mutational hot spot of the most common 4 Chinese deafness genes,namely GJB2(35delG,176del16bp,235delC,299delAT),GJB3(C538T),SLC26A4(IVS72A>G,A2168G)and mitochondrial DNA 12S rRNA(A1555G,C1494T).Further genetic testing were provided to the spouses and newborns of the screened carriers.Results Peripheral blood of 430 pregnant women were detected,detection of deafness gene mutation carriers in 24 cases(4.2%),including 13 cases of the GJB2 heterozygous mutation,3 cases of SLC26A4 heterozygous mutation,1cases of GJB3 heterozygous mutation,and 1 case of mitochondrial 12S rRNA mutation.18 spouses and 17 newborns took further genetic tests,and 6 newborns inherited the mutation from their mother.Conclusion The common deafness genes mutation has a high carrier rate in pregnant women group,235delC and IVS7-2A>G heterozygous mutations are common. | CHANG Liang ZHONG Su ZHAO Nan LIU Ping ZHAO Yangyu QIAO Jie | 2014 | Journal of Otology2014，9，2： | 8 |
| 12 | Resorbable polymer electrospun nanofibers:History,shapes and application for tissue engineering显示文摘Resorbable polymer electrospun nanofiber-based materials/devices have high surface-to-volume ratio and often have a porous structure with excellent pore interconnectivity,which are suitable for growth and development of different types of cells.Due to the huge advantages of both resorbable polymers and electrospun nano fibers,re sorbable polymer electrospun nanofibers(RPENs)have been widely applied in the field of tissue engineering.In this paper,we will mainly introduce RPENs for tissue engineering.Firstly,the electrospinning technique and electrospun nanofiber architectures are briefly introduced.Secondly,the application of RPENs in the field of tissue engineering is mainly reviewed.Finally,the advantages and disadvantages of RPENs for tissue engineering are discussed.This review will provide a comprehensive guide to apply resorbable polymer electrospun nanofibers for tissue engineering. | Tingting Wu Mengzhen Ding Cuiping Shi Yiqun Qiao Panpan Wang Ruirui Qiao Xichang Wang Jian Zhong | 2020 | Chinese Chemical Letters2020，31，3： | 8 |
| 13 | Cordyceps sinensis protects against liver and heart injuries in a rat model of chronic kidney disease: a metabolomic analysis显示文摘 | Xia LIU Fang ZHONG Xu-long TANG Fu-lin LIAN Qiao ZHOU Shan-mai GUO Jia-fu LIU Peng SUN Xu HAO Ying LU Wei-ming WANG Nan CHEN Nai-xia ZHANG | 2014 | Acta Pharmacologica Sinica2014，35，5： | 7 |
| 14 | Chemoprophylaxis, diagnosis, treatments, and discharge management of COVID-19: An evidence-based clinical practice guideline(updated version)显示文摘The novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is the cause of a rapidly spreading illness,coronavirus disease 2019(COVID-19),affecting more than seventeen million people around the world.Diagnosis and treatment guidelines for clinicians caring for patients are needed.In the early stage,we have issued'A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV)infected pneumonia(standard version)';now there are many direct evidences emerged and may change some of previous recommendations and it is ripe for develop an evidence-based guideline.We formed a working group of clinical experts and methodologists.The steering group members proposed 29 questions that are relevant to the management of COVID-19 covering the following areas:chemoprophylaxis,diagnosis,treatments,and discharge management.We searched the literature for direct evidence on the management of COVID-19,and assessed its certainty generated recommendations using the Grading of Recommendations,Assessment,Development and Evaluation(GRADE)approach.Recommendations were either strong or weak,or in the form of ungraded consensus-based statement.Finally,we issued 34 statements.Among them,6 were strong recommendations for,14 were weak recommendations for,3 were weak recommendations against and 11 were ungraded consensus-based statement.They covered topics of chemoprophylaxis(including agents and Traditional Chinese Medicine(TCM)agents),diagnosis(including clinical manifestations,reverse transcription-polymerase chain reaction(RT-PCR),respiratory tract specimens,IgM and IgG antibody tests,chest computed tomography,chest X-ray,and CT features of asymptomatic infections),treatments(including lopinavirritonavir,umifenovir,favipiravir,interferon,remdesivir,combination of antiviral drugs,hydroxychloroquine/chloroquine,interleukin-6 inhibitors,interleukin-1 inhibitors,glucocorticoid,qingfei paidu decoction,lianhua qingwen granules/capsules,convalescent plasma,lung transplantation,invasive or noninvasive ventilation,and extracorporeal membrane oxygenation(ECMO)),and discharge management(including discharge criteria and management plan in patients whose RT-PCR retesting shows SARS-CoV-2 positive after discharge).We also created two figures of these recommendations for the implementation purpose.We hope these recommendations can help support healthcare workers caring for COVID-19 patients. | Ying-Hui Jin Qing-Yuan Zhan Zhi-Yong Peng Xue-Qun Ren Xun-Tao Yin Lin Cai Yu-Feng Yuan Ji-Rong Yue Xiao-Chun Zhang Qi-Wen Yang Jianguang Ji Jian Xia Yi-Rong Li Fu-Xiang Zhou Ya-Dong Gao Zhui Yu Feng Xu Ming-Li Tu Li-Ming Tan Min Yang Fang Chen Xiao-Ju Zhang Mei Zeng Yu Zhu Xin-Can Liu Jian Yang Dong-Chi Zhao Yu-Feng Ding Ning Hou Fu-Bing Wang Hao Chen Yong-Gang Zhang Wei Li Wen Chen Yue-Xian Shi Xiu-Zhi Yang Xue-Jun Wang Yan-Jun Zhong Ming-Juan Zhao Bing-Hui Li Lin-Lu Ma Hao Zi Na Wang Yun-Yun Wang Shao-Fu Yu Lu-Yao Li Qiao Huang Hong Weng Xiang-Ying Ren Li-Sha Luo Man-Ru Fan Di Huang Hong-Yang Xue Lin-Xin Yu Jin-Ping Gao Tong Deng Xian-Tao Zeng Hong-Jun Li Zhen-Shun Cheng Xiao-Mei Yao Xing-Huan Wang | 2020 | Military Medical Research2020，7，3： | 6 |
| 15 | The novel H1N1 Influenza A global airline transmission and early warning without travel containments显示文摘A novel influenza A (H1N1) has been spreading worldwide. Early studies implied that international air travels might be key cause of a severe potential pandemic without appropriate containments. In this study, early outbreaks in Mexico and some cities of United States were used to estimate the preliminary epidemic parameters by applying adjusted SEIR epidemiological model, indicating transmissibility infectivity of the virus. According to the findings, a new spatial allocation model totally based on the real-time airline data was established to assess the potential spreading of H1N1 from Mexico to the world. Our estimates find the basic reproductive number R0 of H1N1 is around 3.4, and the effective reproductive number fall sharply by effective containment strategies. The finding also implies Spain, Canada, France, Panama, Peru are the most possible country to be involved in severe endemic H1N1 spreading. | CHANG ChaoYi CAO ChunXiang WANG Qiao CHEN Yu CAO ZhiDong ZHANG Hao DONG Lei ZHAO Jian XU Min GAO MengXu ZHONG ShaoBo HE QiSheng WANG JinFeng LI XiaoWen | 2010 | Chinese Science Bulletin2010，55，26： | 6 |
| 16 | Preparation and Anti-oxidative Effects of Corn Peptides显示文摘Corn peptides(CP) were prepared from zein via proteolysis by means of a type of alkaline protease, Alcalase, at 50 ℃ and pH 8.0. The anti oxidative activity of the hydrolysate was examined. It was found that the anti oxidative activity reached the maximum at the fourth hour of the hydrolysis and then decreased. The effect of the enzyme dosage on the solubility of corn protein, the dependence of hydrolysis time on the degree of the hydrolysis(DH) and molecular weight distribution were examined respectively. 30% DH was controlled on the basis of anti oxidative activity. The hydrolysate was fractionated on a Sephadex G 15 column. The anti oxidative activities were examined for all the fractions. The main fraction for anti oxidative activity was collected and characterized by the methods of amino acid analysis, the measurement of average chain length and etc .. The main M W distribution of the main fraction was 300-600 Daltons. The anti oxidative properties of the small peptides in vitro were studied. It was found that they could inhibit the auto oxidation of pyrogallol and the oxidation of hemoglobin. The hemolysis degree of erythrocyte caused by hydroxyl free radicals was decreased greatly, indicating a protective effect of the anti oxidative peptides on the cell membrane damage of erythrocyte. | XU Li, ZHANG Xue zhong ** , GUO Yi, REN Qiao and WU Xiao xia Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, P.R. China | 2002 | Chemical Research in Chinese Universities2002，18，3： | 5 |
| 17 | Production of Free Amino Acid and Short Peptide Fertilizer from Rapeseed Meal Fermentation Using Bacillus flexus NJNPD41 for Promoting Plant Growth显示文摘Free amino acids and short peptides(FAPS)that play an important role in plant nutrition can be extracted from rapeseed meal(RSM)by microbial enzymolysis.The enzymolytic activity of one bacterial strain isolated from RSM for the production of FAPS fertilizer(FAPSF)via solid-state fermentation,as well as its detoxification activity for isothiocyanates and oxazolidinethione,was investigated in this study.The strain NJNPD41 isolated from RSM piled-soil possessed effective proteolytic activity and was identified as Bacillus flexus on the basis of its morphological,physiological,and biochemical properties,as well as 16S rDNA analysis.Compared to the uninoculated control,inoculation with the strain NJNPD41 significantly increased the yield of total free amino acids and short peptides by 115%and decreased the content of isothiocyanates and oxazolidinethione by 53%and 60%,respectively,after solid-state fermentation.The pot and field experiments showed that FAPSF significantly promoted eggplant growth and enhanced the fruit yield and quality of eggplant compared to the control.In conclusion,this study provided a novel method for the high-value utilization of RSM to produce high-quality and low-toxicity FAPSF using the newly isolated strain B.flexus NJNPD41. | LIU Hongjun ZHONG Xi HUANG Yan QIAO Cece SHAO Cheng LI Rong SHEN Qirong | 2018 | Pedosphere2018，28，2： | 5 |
| 18 | Atorvastatin Attenuates TNF-alpha Production via Heme Oxygenase-1 Pathway in LPS-stimulated RAW264.7 Macrophages显示文摘Objective To assess the effect of atorvastatin on lipopolysaccharide(LPS)-induced TNF-α production in RAW264.7 macrophages. Methods RAW264.7 macrophages were treated in different LPS concentrations or at different time points with or without atorvastatin. TNF-α level in supernatant was measured. Expressions of TNF-α mRNA and protein and heme oxygenase-1(HO-1) were detected by ELISA, PCR, and Western blot, respectively. HO activity was assayed. Results LPS significantly increased the TNF-α expression and secretion in a dose- and time-dependent manner. The HO-1 activity and HO-1 expression level were significantly higher after atorvastatin treatment than before atorvastatin treatment and attenuated by SB203580 and PD98059 but not by SP600125, suggesting that the ERK and p38 mitogen-activated protein kinase(MAPK) pathways participate in regulating the above-mentioned effects of atorvastatin. Moreover, the HO-1 activity suppressed by SnPP or the HO-1 expression inhibited by siRNA significantly attenuated the effect of atorvastatin on TNF-α expression and production in LPS-stimulated macrophages. Conclusion Atorvastatin can attenuate LPS-induced TNF-α expression and production by activating HO-1 via the ERK and p38 MAPK pathways, suggesting that atorvastatin can be used in treatment of inflammatory diseases such as sepsis, especially in those with atherosclerotic diseases. | WANG Xiao Qiao LUO Nian Sang CHEN Zhong Qing LIN Yong Qing GU Miao Ning CHEN Yang Xin | 2014 | Biomedical and Environmental Sciences2014，27，10： | 4 |
| 19 | Erlotinib versus gemcitabine plus cisplatin as neoadjuvant treatment of stage IIIA-N2 EGFR-mutant non-small-cell lung cancer:final overall survival analysis of the EMERGING-CTONG 1103 randomised phase II trial显示文摘EMERGING-CTONG 1103 showed improved progression-free survival(PFS)with neoadjuvant erlotinib vs.chemotherapy for patients harbouring EGFR sensibility mutations and R0 resected stage IIIA-N2 non-small cell lung cancer(NSCLC)(NCT01407822).Herein,we report the final results.Recruited patients were randomly allocated 1:1 to the erlotinib group(150 mg/day orally;neoadjuvant phase for 42 days and adjuvant phase to 12 months)or to the GC group(gemcitabine 1250 mg/m2 plus cisplatin 75 mg/m2 intravenously;2 cycles in neoadjuvant phase and 2 cycles in adjuvant phase).Objective response rate(ORR),complete pathologic response(pCR),PFS,and overall survival(OS)were assessed along with safety.Post hoc analysis was performed for subsequent treatments after disease recurrence.Among investigated 72 patients(erlotinib,n=37;GC,n=35),the median follow-up was 62.5 months.The median OS was 42.2 months(erlotinib)and 36.9 months(GC)(hazard ratio[HR],0.83;95%confidence interval[CI],0.47-1.47;p=0.513).The 3-and_(5-y)ear OS rates were 58.6%and 40.8%with erlotinib and 55.9%and 27.6%with GC(p_(3-y)=0.819,p_(5-y)=0.252).Subsequent treatment was administered in 71.9%and 81.8%of patients receiving erlotinib and GC,respectively;targeted therapy contributed mostly to OS(HR,0.35;95%CI,0.18-0.70).After disease progression,the ORR was 53.3%,and the median PFS was 10.9 months during the EGFR-TKI rechallenge.During postoperative therapy,grade 3 or 4 adverse events(AEs)were 13.5%in the erlotinib group and 29.4%in the GC group.No serious adverse events were observed.Erlotinib exhibited clinical feasibility for resectable IIIA-N2 NSCLC over chemotherapy in the neoadjuvant setting. | Wen-Zhao Zhong Hong-Hong Yan Ke-Neng Chen Chun Chen Chun-Dong Gu Jun Wang Xue-Ning Yang Wei-Min Mao Qun Wang Gui-Bin Qiao Ying Cheng Lin Xu Chang-Li Wang Ming-Wei Chen Xiao-Zheng Kang Wan-Pu Yan Ri-Qiang Liao Jin-Ji Yang Xu-Chao Zhang Si-Yang Liu Qing Zhou Yi-Long Wu | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 4 |
| 20 | Effects of Cerium on Microstructures and Mechanical Properties of Zn-22%Al Vibration Damping Alloy显示文摘Using natural aging and artificial aging treatment, the effects of cerium on microstructures and mechanical properties of Zn 22%Al vibration damping alloy were studied by optical microscope, transmission electron microscope and scanning electron microscope. The results show that cerium can refine the microstructure of Zn 22%Al alloy, slow the grain growth and retain the equiaxial degree during aging. The strength of Zn 22%Al (0 07%~0 20%)Ce alloy and Zn 22%Al alloy remains constant after aging below the hot rolling temperature of 80 ℃, and cerium can improve the strength and the stability of mechanical properties of Zn 22%Al alloy. | PANG Shao ping, SHI Yun bao, LI Jun, YANG Bin jiu, QUAN Mei hua, QIAO Yan hui, ZHANG Chi zhong (College of Mechanical Engineering, Beihua University, Jilin 132021, China) | 2000 | Journal of Rare Earths2000，18，4： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员