| 1 | Transplant outcomes of 100 cases of living-donor ABO-incompatible kidney transplantation显示文摘Background:Although ABO-incompatible(ABOi)kidney transplantation(KT)has been performed successfully,a standard preconditioning regimen has not been established.Based on the initial antidonor ABO antibody titers,an individualized preconditioning regimen is developed,and this study explored the efficacy and safety of the regimen.Methods:From September 1,2014,to September 1,2020,we performed 1668 consecutive living-donor KTs,including 100 ABOi and 1568 ABO-compatible(ABOc)KTs.ABOi KT recipients(KTRs)with a lower antibody titer(≤1:8)were administered oral immunosuppressive drugs(OIs)before KT,while patients with a medium titer(1:16)received OIs plus antibody-removal therapy(plasma exchange/double-filtration plasmapheresis),patients with a higher titer(≥1:32)were in addition received rituximab(Rit).Competing risk analyses were conducted to estimate the cumulative incidence of infection,acute rejection(AR),graft loss,and patient death.Results:After propensity score analyses,100 ABOi KTRs and 200 matched ABOc KTRs were selected.There were no significant differences in graft and patient survival between the ABOi and ABOc groups(P=0.787,P=0.386,respectively).After using the individualized preconditioning regimen,ABOi KTRs showed a similar cumulative incidence of AR(10.0%vs.10.5%,P=0.346).Among the ABOi KTRs,the Rit-free group had a similar cumulative incidence of AR(P=0.714)compared to that of the Rittreated group.Multivariate competing risk analyses revealed that a Rit-free regimen reduced the risk of infection(HR:0.31;95%CI:0.12–0.78,P=0.013).Notably,antibody titer rebound was more common in ABOi KTRs receiving a Rit-free preconditioning regimen(P=0.013)than those receiving Rit.ABOi KTRs with antibody titer rebound had a 2.72-fold risk of AR(HR:2.72,95%CI:1.01–7.31,P=0.048).ABOi KTRs had similar serum creatinine and estimated glomerular filtration rate compared to those of ABOc KTRs after the first year.Conclusions:An individualized preconditioning regimen can achieve comparable graft and patient survival rates in ABOi KT with ABOc KT.Rit-free preconditioning effectively prevented AR without increasing the risk of infectious events in those with lower initial titers;however,antibody titer rebound should be monitored. | Saifu Yin Qiling Tan Youmin Yang Fan Zhang Turun Song Yu Fan Zhongli Huang Tao Lin Xianding Wang | 2022 | Chinese Medical Journal2022，135，19： | 7 |
| 3 | Integration of semi-in vivo assays and multi-omics data reveals the effect of galloylated catechins on self-pollen tube inhibition in Camellia oleifera显示文摘Camellia oil extracted from the seeds of Camellia oleifera Abel.is a popular and high-quality edible oil,but its yield is limited by seed setting,which is mainly caused by self-incompatibility(SI).One of the obvious biological features of SI plants is the inhibition of self-pollen tubes;however,the underlying mechanism of this inhibition in C.oleifera is poorly understood.In this study,we constructed a semi-in vivo pollen tube growth test(SIV-PGT)system that can screen for substances that inhibit self-pollen tubes without interference from the genetic background.Combined with multi-omics analysis,the results revealed the important role of galloylated catechins in self-pollen tube inhibition,and a possible molecular regulatory network mediated by UDP-glycosyltransferase(UGT)and serine carboxypeptidase-like(SCPL)was proposed.In summary,galloylation of catechins and high levels of galloylated catechins are specifically involved in pollen tube inhibition under self-pollination rather than cross-pollination,which provides a new understanding of SI in C.oleifera.These results will contribute to sexual reproduction research on C.oleifera and provide theoretical support for improving Camellia oil yield in production. | Yihong Chang Wenfang Gong Jinming Xu Han Gong Qiling Song Shixin Xiao Deyi Yuan | 2023 | Horticulture Research2023，10，1： | 1 |
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