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5篇 您的检索式:作者名="Qingyun Diao"
    题名 作者 年代 出处 被引量
1Simultaneous determination of trace migration of phthalate esters in honey and royal jelly by GC–MS显示文摘Jinhui Zhou Yitao Qi Hongmei Wu Qingyun Diao Feifei Tian Yi Li 2014J Sep Science2014,,6:1
2Heat transfer in ground heat exchangers with groundwater advection 显示文摘Diao Nairen Li Qingyun Fang Zhaohong 2004International Journal of Thermal Sciences2004,43,12:1
3Heat Transfer in Ground Heat Exchangers with Groundwater Advection显示文摘Diao Nairen Li Qingyun Fang Zhaohong 2004International Journal of Thermal Sciences2004,43,12:1
4Heat Transfe in Ground Heat Exchangers with Gr oundwater Advection显示文摘Diao Nairen Li Qingyun Fang Zhaohong 2004International Journal of Thermal Science2004,43,12:1
5Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China显示文摘Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV.Dahe Yang Jun Wang Xi Wang Fei Deng Qingyun Diao Manli Wang Zhihong Hu Chunsheng Hou 2022Virologica Sinica2022,37,4:1
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