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| 1 | Assessing bacterial communities in the rhizosphere of 8-year-old genetically modified poplar(Populus spp.)显示文摘Microbe communities in rhizosphere ecosystems are important for plant health but there is limited knowledge of them in the rhizospheres of genetically modified(GM) plants, especial for tree species. We used the amplitude sequencing method to analyze the V4 regions of the 16 S r RNA gene to identify changes in bacterial diversity and community structure in two GM lines(D520 and D521), one non-genetically modified(nonGM) line and in uncultivated soil. After chimera filtering,468.133 sequences in the domain Bacteria remained. There were ten dominant taxonomic groups(with [1 % of all sequences) across the samples. 241 of 551 genera(representing a ratio of 97.33 %) were common to all samples.A Venn diagram showed that 1.926 operational taxonomic units(OTUs) were shared by all samples. We found a specific change, a reduction in Chloroflexi, in the microorganisms in the rhizosphere soil planted with poplars. Taken together, the results showed few statistical differences in the bacterial diversity and community structure between the GM line and non-GM line, this suggests that there was no or very limited impact of this genetic modification on the bacterial communities in the rhizosphere. | Wenxu Zhu Yanguang Chu Changjun Ding Qinjun Huang Bingyu Zhang Weixi Zhang Xiaohua Su | 2016 | Journal of Forestry Research2016，27，4： | 2 |
| 2 | Relationship between perioperative inflammatory response and postoperative cognitive dysfunction in the elderly显示文摘 | GuangLun Xie Wei Zhang YanZi Chang QinJun Chu | 2009 | Medical Hypotheses2009，，3： | 1 |
| 3 | Morphological and physiological plasticity response to low nitrogen stress in black cottonwood(Populus deltoides Marsh.)显示文摘It is important to evaluate nitrogen use efficiency and nitrogen tolerance of trees in order to improve their productivity.In this study,both were evaluated for 338 Populus deltoides genotypes from six provenances.The plants were cultured under normal nitrogen(750μM NH_4 NO_3)and low nitrogen(5μM NH_4 NO_3)conditions for 3 months.Growth,chlorophyll content and glutamine synthetase activity of each genotype were measured.Under low nitrogen,heights,ground diameter,leaf area,leaf and root biomass,and chlorophyll contents were significantly lower than those under normal nitrogen level.Correlation analysis showed that nutrient distribution changed under different nitrogen treatments.There was a negative correlation between leaf traits and root biomass under normal nitrogen level,however,the correlation became positive in low nitrogen treatment.Moreover,with the decrease of nitrogen level,the negative correlation between leaf morphology and chlorophyll levels became weakened.The growth of the genotypes under the two treatments was evaluated by combining principal component analysis with a fuzzy mathematical membership function;the results showed that leaf traits accounted for a large proportion of the variation in the evaluation model.According to the results of comprehensive evaluation of plants under the two treatments,the 338 P.deltoides genotypes could be divided into nine categories,with wide genotypic diversity in nitrogen use efficiency and low nitrogen tolerance.As a result,26 N-efficient genotypes and 24 N-inefficient genotypes were selected.By comparative analysis of their morphological and physiological traits under the two treatments,leaf traits could be significant indicators for nitrogen use efficiency and nitrogen tolerance,which is of considerable significance for breeding poplar varieties with high nitrogen use efficiencies. | Cun Chen Yanguang Chu Qinjun Huang Changjun Ding Weixi Zhang Bo Li Jing Zhang Xiaohua Su | 2022 | Journal of Forestry Research2022，33，1： | 1 |
| 4 | EMLA■ Cream coated on endotracheal tube with or without epidural lidocaine reduces isoflurane requirement during general anesthesia显示文摘This study was designed to evaluate the effect of tracheal topical anesthesia using EMLA■Cream(EC)coated on the endotracheal tube(ETT)with or without epidural anesthesia(EA)on isoflurane requirement during general anesthesia(GA)and investigate whether EC coated on the ETT with EA was associated with the additive effect compared with the effect when each anesthetic was administered independently.The prospective randomized,double-blinded,and controlled study included 60 ASA Ⅰ-Ⅱ patients scheduled for upper abdominal surgery requiring GA.Patients were randomly assigned to one of the following groups:group 1 received GA,group 2 received EC+GA,group 3 received GA+EA,and group 4 received EC+GA+EA.Isoflurane was administered at the required concentrations to maintain the mean arterial pressure at a level not exceeding 20% of preoperative values.The percentage mean expired concentration(%MEC)was used in calculating the isoflurane requirement.Emergence agitation,postoperative sore throat,and hoarseness were recorded.Groups 2,3,and 4 exhibited a significant reduction on isoflurane requirement compared with group 1(P<0.05).The isoflurane requirement evaluated by%MEC decreased by 12%,38%,and 50% in groups 2,3,and 4,respectively.The incidence of emergence agitation was significantly lower in groups 2 and 4 than those in groups 1 and 3(P<0.05).Tracheal topical anesthesia using EC coated on ETT with or without EA reduced the isoflurane requirement during GA,indicating that EC combined with EA exhibited an additive effect on the requirement of general anesthetic. | Hai Yu Qinjun Chu Jin Liu Li Chen Ying Wang Yunxia Zuo | 2012 | Frontiers of Medicine2012，6，3： | 1 |
| 5 | Microbial Electrosynthesis for Producing Medium Chain Fatty Acids显示文摘Microbial electrosynthesis(MES)employs microbial catalysts and electrochemistry to enhance CO_(2)bioconversion to organics with concurrent waste biorefining capability.The aim of this review is to comprehensively discuss the current state of the art and prospects of medium chain fatty acids(MCFAs)production in MES from CO_(2)and organic wastes.Fundamental mechanisms and development of MCFAs production via conventional fermentation are introduced as well.Studies on MCFAs production in MES are summarized,highlighting the strategy of multiple-electron donors(EDs).Challenges for MCFAs production in MES from CO_(2)are presented,and the primary discussions included methanogenesis inhibition,adenosine triphosphate(ATP)limitations of acetogens,and production of limited EDs via solventogenesis.Possible applications of electrochemical approaches to promote the bioconversion of actual waste materials with MCFAs production are analyzed.Finally,future directions are explored,including multi-stage reactions,substrate supply,product extraction,and microbial pathways. | Na Chu Wen Hao Qinglian Wu Qinjun Liang Yong Jiang Peng Liang Zhiyong Jason Ren Raymond Jianxiong Zeng | 2022 | Engineering2022，，9： | 0 |
| 6 | Nanomaterials with dual immunomodulatory functions for synergistic therapy of breast cancer brain metastases显示文摘A long-standing paucity of effective therapies results in the poor outcomes of triple-negative breast cancer brain metastases.Immunotherapy has made progress in the treatment of tumors,but limited by the non-immunogenicity of tumors and strong immunosuppressive environment,patients with TNBC brain metastases have not yet benefited from immunotherapy.Dual immunoregulatory strategies with enhanced immune activation and reversal of the immunosuppressive microenvironment provide new therapeutic options for patients.Here,we propose a cocktail-like therapeutic strategy of microenvironment regulation-chemotherapy-immune synergistic sensitization and construct reduction-sensitive immune microenvironment regulation nanomaterials(SIL@T).SIL@T modified with targeting peptide penetrates the BBB and is subsequently internalized into metastatic breast cancer cells,releasing silybin and oxaliplatin responsively in the cells.SIL@T preferentially accumulates at the metastatic site and can significantly prolong the survival period of model animals.Mechanistic studies have shown that SIL@T can effectively induce immunogenic cell death of metastatic cells,activate immune responses and increase infiltration of CD8+T cells.Meanwhile,the activation of STAT3 in the metastatic foci is attenuated and the immunosuppressive microenvironment is reversed.This study demonstrates that SIL@T with dual immunomodulatory functions provides a promising immune synergistic therapy strategy for breast cancer brain metastases. | Zhenhao Zhao Chufeng Li Yiwen Zhang Chao Li Yongchao Chu Xuwen Li Peixin Liu Hongyi Chen Yu Wang Boyu Su Qinjun Chen Tao Sun Chen Jiang | 2023 | Bioactive Materials2023，，9： | 0 |
| 7 | Establishment of Efficient Regeneration System of Populus×euramericana cl.‘Bofeng’显示文摘Compared with the species of Leuce section,the difficulty in plant regeneration during tissue culture for the species of Aigeiros section is a key limiting factor for their application in genetic engineering of forest trees.In this study,the plant growth regulators combination,copper concentration,light intensity,and the selection pressures of kanamycin were investigated using leaves of Populus×euramericana cl.’Bofeng’ as explants,and a stable and high efficient regeneration system was established.Using this system,both the shoot regeneration rate and rooting rate were up to 100%,and the average number of differentiated shoots in each leaf explant was up to 20,and the survival rate of seedlings reached 98%.The optimal selection pressure of kanamycin in shoot inducement for leaf-explant was 40 mg·L-1,and the optimal selection pressure of kanamycin in root inducement of adventitious bud was 20 mg·L-1.The authors also found that differentiation rate of adventitious buds of P.×euramericana cl.’Bofeng’ could be significantly promoted by increased copper concentration,and this is the first report about the key role of copper(Cu) in explant regeneration of Poplars.This regeneration system with high frequency which was established by the authors was as good as that of species of Leuce section,and it provides a good technological platform for genetic engineering of forest tree which uses the species of Aigeiros section as model materials. | Cui Xudong Su Xiaohua Zhang Bingyu Chu Yanguang Huang Qinjun Zhang Weixi Liu Boyang | 2012 | Chinese Forestry Science and Technology2012，11，3： | 0 |
| 8 | Electricity-driven ammonia oxidation and acetate production in microbial electrosynthesis systems显示文摘Microbial electrosynthesis(MES)is an emerging technology for producing chemicals,and coupling MES to anodic waste oxidation can simultaneously increase the competitiveness and allow additional functions to be explored.In this study,MES was used for the simultaneous removal of ammonia from synthetic urine and production of acetate from CO_(2).Using graphite anode,83.2%±5.3%ammonia removal and 28.4%±9.9%total nitrogen removal was achieved,with an energy consumption of 1.32 kWh/g N for total nitrogen removal,0.45 kWh/g N for ammonia nitrogen removal,and 0.044 kWh/g for acetate production.Using boron-doped diamond(BDD)anode,70.9%±12.1%ammonia removal and 51.5%±11.8%total nitrogen removal was obtained,with an energy consumption of 0.84 kWh/g N for total nitrogen removal,0.61 kWh/g N for ammonia nitrogen removal,and 0.043 kWh/g for acetate production.A difference in nitrate accumulation explained the difference of total nitrogen removal efficiencies.Transport of ammonia and acetate across the membrane deteriorated the performance of MES.These results are important for the development of novel elcctricity-driven technologies for chemical production and pollution removal. | Qinjun Liang Yu Gao Zhigang Li Jiayi Cai Na Chu Wen Hao Yong Jiang Raymond Jianxiong Zeng | 2022 | Frontiers of Environmental Science & Engineering2022，16，4： | 0 |
| 9 | A cDNA microarray analysis of the molecular control of poplar wood properties显示文摘Molecular biological research into wood development and formation has been the focus in recent years,but the pace of discovery of related genes and their functions in the control of wood properties has been slow. The microarray technique—with its advantages of high throughput capacity, sensitivity, and reliability over other tools developed for investigating genes expression patterns—is capable of rapidly assaying thousands of genes.In this study, a cDNA microarray prepared from two cDNA libraries of developing poplar xylem tissues was used to assay gene expression patterns in immature xylem tissues at different heights from the main stem of Populus deltoides(15 years old), which was confirmed to have distinct wood properties(microfibrillar angle, woody density) by X-ray. Two hundred seventy-four transcripts with differential expression profiles between the chips were screened out, and the individual clones were subjected to 5' sequencing. Using bioinformatic analysis, we identifiedcandidate genes that may influence poplar wood properties,many of which belong to various regulatory and signal transduction gene families, such as zinc finger protein transcription factor, DNA-binding transcription factor,ethylene response factors, and so on. The results suggest that these genes may regulate enzymes involved in wood formation. Further work will be performed to clone these genes and determine how they influence poplar wood properties. | Qinjun Huang Changjun Ding Weixi Zhang Bingyu Zhang Yanguang Chu Dahai Wang Xiaohua Su | 2017 | Journal of Forestry Research2017，28，1： | 0 |
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