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| 1 | Oncofetal antigen glypican-3 as a promising early diagnostic marker for hepatocellular carcinoma显示文摘BACKGROUND:Hepatocellular carcinoma (HCC) is characterized by a multi-cause,multi-stage and multi-focus process of tumor progression.Its prognosis is poor and early diagnosis is of utmost importance.This study was undertaken to investigate the dynamic expression of oncofetal antigen glypican-3 (GPC-3) and GPC-3 mRNA in hepatocarcinogenesis and to explore their early diagnostic value for HCC.METHODS:A hepatoma model was induced in male Sprague-Dawley rats with 0.05% 2-fluorenylacetamide and confirmed by hematoxylin and eosin staining and gamma-glutamyltransferase (GGT) expression.Total RNA was purified and transcribed into cDNA by reverse transcription.Fragments of the GPC-3 gene were amplified by nested RT-PCR,and confirmed by sequencing.GPC-3 was analyzed by immunohistochemistry,Western blotting or ELISA.RESULTS:Positive GPC-3 expression showed as brown granule-like staining localized in the cytoplasm.Histological examination of hepatocytes revealed three morphological stages of granule-like degeneration,atypical hyperplasia (precancerous),and cancer formation,with a progressive increase of liver total RNA and GGT expression.The incidence of liver GPC-3 mRNA and GPC-3,and serum GPC-3 was 100%,100% and 77.8% in the HCC group,100%,100%,and 66.7% in the precancerous group,83.3%,83.3%,and 38.9% in the degeneration group,and no expression in the liver or blood of the control group,respectively.There was a positive correlation between liver GPC-3 mRNA and total RNA level (r=0.475,P<0.05) or liver GPC-3 (r=1.0,P<0.001) or serum GPC-3 (r= 0.994,P<0.001).CONCLUSION:Abnormal oncofetal antigen GPC-3 and GPC-3 mRNA expression in hepatocarcinogenesis may be promising molecular markers for early diagnosis of HCC. | Research Center of Clinical Medicine,Affiliated Hospital of Nantong University,Nantong 226001,China (Yao M,Yao DF,Qiu LW,Wu W,Sai WL,Yang JL and Zhang HJ) Department of Oncology,Yancheng First People’s Hospital,Yancheng 224001,China (Bian YZ) Department of Oncology,Second Affiliated Hospital,Nanjing Medical University,Nanjing 210011,China (Zhang CG) | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 51 |
| 2 | Human γδT-cell subsets and their involvement in tumor mmunity显示文摘 | Dang W u Pin Wu Fuming Qiu Qichun Wei Jian Huang | 2017 | Cellular & Molecular Immunology2017，14，3： | 11 |
| 3 | Intense single-band red upconversion emission in BiOCl:Er3+ layered semiconductor via co-doping Ho3+显示文摘Exploring a new tuning way to facilely realize single-band red emission in trivalent rare-earth ions(RE3+) doped upconversion(UC) materials is still desirable.In this work,the intense single-band red emission is achieved by co-doping only Ho3+in the BiOCl:Er3+ under 1550 nm excitation.In the BiOCl layered host,co-doping Ho3+can further enhance the red emission and simultaneously suppress the green emission of Er3+,and thus obviously improve the red-to-green(R/G) ratio.It is found that Ho3+does not se rve as ene rgy trapping through the 5 I6 state as in traditional UC materials but acts as ET bridge(4 S3/2,2 H11/2(Er3+)→5 F4,5 S2(Ho3+)→4 F9/2(Er3+)).The tuning mechanism of Ho3+is discussed in detail and further confirms through a comparative experiment.Our research gives an unusual perspective to tune the UC behavior of Er3+through co-doping Ho3+,which might be inspiring for achievement of single-band red UC emission. | Yuehong Peng Jincai Peng Jiajun Han Tianhui Wang Zhaoyi Yin Jianbei Qiu Qi W ang Zhengwen Yang Zhiguo Song | 2020 | Journal of Rare Earths2020，38，6： | 2 |
| 4 | Epidemiology of respiratory viruses in patients hospitalized with near-fatal asthma, acute exacerbations of asthma, or chronic obstructive pulmonary disease显示文摘 | Tan W C Xiang X Qiu D | 2003 | Am J Med2003，115，4： | 2 |
| 5 | Polychlorinated biphenyl congeners and organochlorine insecticides in the water column and sediments of Daya Bay, China 显示文摘 | Zhou J L Maskaoui K Qiu Y W | 2001 | Environmental Pollution2001，113，： | 2 |
| 6 | Enhanced high-potential and elevated-temperature cycling stability of LiMn2O4 cathode by TiO2 modification for Li-ion battery 显示文摘 | Yu L Qiu x xi J Zhu W T Chen L Q | 2006 | Eleetrochimica Acta2006，51，： | 1 |
| 7 | Development of an antigen capture immunoassay based on monoclonal antibodies specific for dengue virus serotype 2 nonstructural protein 1 for early and rapid identification of dengue virus serotype 2 infections显示文摘 | Qiu L W Di B Wen K | 2009 | Clin Vaccine Immunol2009，16，： | 1 |
| 8 | Time-frequency imaging algorithm for high speed spinning targets in two dis- mensions 显示文摘 | Li J Qiu C W Xing M D Bao Z Yeo T S | 2010 | lET2010，4，6： | 1 |
| 9 | Performance of RBF neural networks for array processing in impulsive noise environment显示文摘 | Guo W Q Qiu T S Tang H | 2008 | Digital Signal Processing2008，18，2： | 1 |
| 10 | Oxidation resistant SiC coating for graphite materials显示文摘 | Zhu Q S Qiu X L Ma C W | 1999 | Carbon1999，37，9： | 1 |
| 11 | Real-time data-reusing adaptive learning of a radial basis function network for tracking evoked potentials 显示文摘 | Qiu W Chang C Liu W | 2006 | IEEE Trans Biomed Eng2006，53，2： | 1 |
| 12 | Detection and differen- tiation of CAV-1 and CAV-2 by polymerase chain rea- ction显示文摘 | Hu R L Huang G Qiu W | 2001 | Vet Res Commun2001，25，： | 1 |
| 13 | Synthesis of star- shaped poly (D, L-lactic acid-alt-glycolic acid)-b-poly(L- lactic acid) with the poly (D, L-lactic acid-alt-glycolic acid) macroinitiator and stannous octoate catalyst 显示文摘 | Dong C M Qiu K Y Gu Z W | 2002 | Journal of Polymer Science Part A: Polymer Chemistry2002，40，3： | 1 |
| 14 | Contrasting geochemical and Sm- Nd isotopic compositions of Archean metasediments from the Kongling high-grade terrain of the Yangtze craton: Evidence for cra- tonic evolution and redistribution of REE during crustal anatexis显示文摘 | Gao S Ling W L Qiu Y M | 1999 | Geochimica et Cosmochimica Acta1999，63，1314： | 1 |
| 15 | Application of the kurtosis statistic to the evaluation of the risk of hearing loss in workers exposed to high-level complex noise 显示文摘 | ZHAO Y M QIU W ZENG L | 2010 | Ear Hear2010，31，4： | 1 |
| 16 | Alcohol consumption and incidence of dementia in a community sample aged 75 years and older显示文摘 | Huang W Qiu C Winblad B | 2002 | J Clin Epidemiol2002，55，10： | 1 |
| 17 | Antibodies target microvessels in neuromyelitis optica and multiple sclerosis patients显示文摘 | LONG Y GAO C QIU W | 2013 | Neurol Res2013，35，9： | 1 |
| 18 | Estimating nutrient budgets for prescribed thinning in a regrowth eucalyptus forest in south-west Australia显示文摘 | Qiu S Bell R W Hobbs R J | 2012 | Forestry2012，85，1： | 1 |
| 19 | Arabidopsis mitogen - activated protein kinase kinases MKK1 and MKK2 have overlapping functions in defense signaling mediated by MEKK1, MPK4, and MKS1 显示文摘 | Qiu J L Zhou L Yun B W | 2008 | Plant Physiology2008，148，1： | 1 |
| 20 | The efficacy and com- plications of posterior hemivertebra resection显示文摘 | Zhang J Shengru W Qiu G | 2011 | Eur Spine J2011，20，： | 1 |
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