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您的检索式：作者名="Quanshuang Li"
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| 1 | Neuron-like differentiation of adult rat bone marrow stromal cells induced by transforming growth factor-beta and brain-derived neurotrophic factor显示文摘BACKGROUND:It has been demonstrated that transforming growth factor-β(TGF-β) and brain-derived neurotrophic factor(BDNF) can induce stem cell differentiation into neuron-like cells. OBJECTIVE:To investigate the efficacy of TGF-βand BDNF at inducing the differentiation of adult rat bone marrow stromal cells(BMSCs) into neuron-like cells,both in combination or alone. DESIGN,TIME AND SETTING:A comparative observation experiment was performed at the Department of Orthopedics,First Affiliated Hospital of Liaoning Medical University between October 2007 and January 2008. MATERIALS:TGF-βand BDNF were purchased from Sigma,USA;mouse anti-rat neuron specific enolase,neurofilament and glial fibrillary acidic protein were purchased from Beijing HMHL Biochem Ltd.,China. METHODS:BMSCs were isolated from rats aged 4 weeks and incubated with TGF-β(1μg/L) and/or BDNF(50μg/mL). MAIN OUTCOME MEASURES:Expression of neuron-specific enolase,neurofilament and glial fibrillary acidic protein were determined by immunocytochemistry. RESULTS:BMSCs differentiated into neuron-like cells following induction of TGF-βand BDNF, and expressed both neuron-specific enolase and neurofilament.The percent of positive cells was significantly greater in the combination group than those induced with TGF-βor BDNF alone(P<0.01). CONCLUSION:Treatment of BMSCs with a combination of TGF-βand BDNF induced differentiation into neuron-like cells,with the induction being significantly greater than with TGF-βor BDNF alone. | Chang Liu Xifan Mei Gang LU Yansong Wang Quanshuang Li Zhanpeng Guo | 2009 | Neural Regeneration Research2009，4，4： | 1 |
| 2 | Can muscle-derived stem cells serve as seed cells to repair spinal cord injury?显示文摘Muscle-derived stem cells(MDSCs) can come from a number of different sources,which are easy to isolate and culture,and are also useful in the transformation and expression of exogenous genes.Therefore,MDSCs could possibly be used for gene therapy in the treatment of neurological diseases.However,research on MDSCs has focused on identifying phenotypes and induced differentiation,with few in vivo animal experiments conducted.In this study,MDSCs were selected as seed cells and implanted into the rat spinal cord injury area.Results demonstrated that the MDSCs survived,migrated,and were distributed along the spinal nerves.Moreover,the motor function of rat lower limbs improved significantly,suggesting that MDSCs could be used as seed cells to repair spinal cord injury. | Xifan Mei Chang Liu Gang Lv Yansong Wang Quanshuang Li Zhanpeng Guo Shiqiong Liu He Zhang | 2010 | Neural Regeneration Research2010，5，19： | 1 |
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