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13篇 您的检索式：作者名="Quenee"

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		题名	作者	年代	出处	被引量
	1	Minisatellite polymorphism as a tool to distinguish closely related Lactococcus lactis strains显示文摘	QUENEE P LEPAGE E KIM W S	2005	FEMS Microbiol Lett2005，248，：	1
	2	Prevention of pneumonic plague in mice, rats, guinea pigs and non-human primates with clinical grade rV10, rV-10-2 or pneumonic F1-V vaccines 显示文摘	Quenee LE Ciletti NA Elli D et d	2011	Vaccine2011，38，29：	1
	3	Molecular quantification of lactic acid bacteria in fermented milk products using real-time quantitative PCR显示文摘	FURET J P QUENEE P TAILLIEZ P	2004	International Journal of Food Microbiology2004，97，2：	1
	4	Orfl/SpcS chaper-ones ExoS for type three secretion by Pseudomonas aeruginosa显示文摘	SHEN D K QUENEE L BONNET M	2008	Biomed Environ Sci2008，21，2：	1
	5	Yersinia pestis caf1 variants and the limits of plague vaccine protection显示文摘	Quenee LE Cornelius CA Ciletti NA	2008	Infect Immun2008，76，5：	1
	6	Orf1/SpcS Chaperones ExoS for Type Three Secretion by Pseudomonas aeruginosa显示文摘Objective Pseudomonas aeruginosa is a ubiquitous and opportunistic pathogen that uses the type III secretion system (TTSS) to inject effector proteins directly into the cytosol of target cells to subvert the host cell’s functions. Specialized bacterial chaperones are required for effective secretion of some effectors. To identify the chaperone of ExoS, the representative effector secreted by the TTSS of P. aeruginosa, we analyzed the role of a postulated chaperone termed Orf1. Methods By allelic exchange, we constructed the mutant with the deletion of gene Orf1. Analysis of secreted and cell-associated fractions was performed by SDS-PAGE and Western blotting. Using strain expressing in trans Orf1, tagged by V5 polypeptide and histidine, protein-protein interaction was determined by affinity resin pull-down assay in combination with MALDI-TOF. The role of Orf1 in the expression of exoS was evaluated by gene reporter analysis. Results Pull-down assay showed that Orf1 binds to ExoS and ExoT. Secretion profile analysis showed that Orf1 was necessary for the optimal secretion of ExoS and ExoT. However, Orf1 had no effect on the expression of exoS. Conclusion Orf1 is important for the secretion of ExoS probably by maintaining ExoS in a secretion-competent conformation. We propose to name Orf1 as SpcS for 'specific Pseudomonas chaperone for ExoS'.	DA-KANG SHEN LAURIANE QUENEE MARIETTE BONNET LAURIANE KUHN MADIHA DEROUAZI DANIELE LAMOTTE BERTRAND TOUSSAINT BENOIT POLACK	2008	Biomedical and Environmental Sciences2008，21，2：	1
	7	Protective immunity against plague显示文摘	Cornelius C Quenee L Anderson D	2007	Adv Exp Med Biol2007，603，4：	1
	8	Molecular quantification of lac- tic acid bacteria in fermented milk products using real-time quantitative PCR显示文摘	FURET JP QUENEE P TAILLIEZ P	2004	Int J Food Mlcrobiol2004，97，2：	1
	9	Molecular quantification of lactic acid bacteria in fermented milk products using real- time quantitative PCR显示文摘	Furet J P Quenee P Tailliez P	2004	Int J Food Microbiol2004，97，2：	1
	10	Combined sacB-based negative selection and cre-lox antibiotic marker recyclingfor efficient gene deletion in Pseudomonas aeruginosa显示文摘	Quenee L Lamotte D Polack B	2005	Biotechniques2005，38，1：	1
	11	Behaviour of cement-treated MSWI bottom ash显示文摘	PECQUEUR G CRIGNON C QUENEE B	2001	Waste Management2001，21，3：	1
	12	Yersinia pestis caf1 variants and the limits of plague vaccine protection显示文摘	Quenee LE Cornelius CA Ciletti NA		0，，05：	1
	13	Molecular quantification of lactic acid bacteria in fermented milk products using real-time quantita- tive PCR 显示文摘	Furet JP Quenee P Tailliez P	2004	International Journal of Food Microbiology2004，97，2：	1

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