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| 1 | 用PCR-RFLP技术鉴别嗜人按蚊和中华按蚊的研究显示文摘目的 依据嗜人按蚊和中华按蚊rDNA的ITS2区段基因特征 ,建立一种新的嗜人按蚊和中华按蚊基因鉴别技术。方法 对不同地区的嗜人按蚊、可疑嗜人按蚊和中华按蚊样本通过采用特异性ITS2 引物PCR扩增 ,限制性内切酶RsaI和HinfI消化 ,以及琼脂糖凝胶电泳分析进行PCR -RFLP基因鉴别。 结果 不同地区嗜人按蚊rDNA的ITS2 基因PCR扩增产物能被限制性内切酶HinfI酶切 ,并显示一条 4 5 0bp的酶切DNA条带 ;中华按蚊的ITS2 基因PCR扩增产物则能被限制性内切酶RsaI酶切 ,并显示 4 0 0bp和 2 0 0bp两条酶切DNA条带 ;辽宁可疑嗜人按蚊的PCR -RFLP结果与嗜人按蚊相同而广东珠海可疑嗜人按蚊的PCR -RFLP结果与中华按蚊相同。结论 依据rDNA的ITS2 区段基因特征建立的PCR -RFLP技术可用于嗜人按蚊和中华按蚊的基因鉴别。采用该PCR -RFLP基因鉴别技术发现辽宁可疑嗜人按蚊的基因与中国大陆的嗜人按蚊属同种 ,而广东可疑嗜人按蚊的基因与中华按蚊属同种。 | 高琪 R D Cooper 周华云 潘波 杨文 郭传坤 黄光全 李凤华 李菊林 沈宝祥 Qin Cheng | 2002 | 中国人兽共患病杂志2002，18，6： | 15 |
| 2 | Nat Genet:单基因突变导致过敏性皮炎的发生显示文摘最近,研究者们鉴定出了一类导致神经性皮炎发生的关键基因突变:CARDll。来自美国NIH过敏与传染病研究所的研究者们通过对四个没有血缘关系的患病家庭进行分析,发现了这一导致疾病产生的基因、 | Chi A Ma, Yuan Zhang, Michael A Weinreich, Jonathan J Lyons, Celeste G Nelson, Thomas DiMaggio, Kelly D Stone, Joshua D Milner Jeffrey R Stinson, Elisa Ruffo, Batsukh Dorjbal, Swadhinya Arjunaraja, Kelsey Voss, Andrew L Snow Jordan K Abbott, Pia J Hauk, Paul R Reynolds, Erwin W Gelfand Elisa Ruffo Salomé Glauzy, Natsuko Yamakawa, Eric Meffre Jennifer Stoddard, Julie Niemela, Sergio D Rosenzweig Yu Zhang, Helen F Matthews Joshua J McElwee Nina Jones Alejandro Palma, Matías Oleastro, Emma Prieto, Andrea R Bernasconi, Geronimo Dubra, Silvia Danielian, Jonathan Zaiat, Marcelo A Marti Brian Kim Megan A Cooper Neil Romberg | 2017 | 现代生物医学进展2017，17，27： | 3 |
| 3 | Euler Deconvolution with Improved Accuracy and Multiple Different Structural Indices显示文摘Euler deconvolution is a semi-automatic interpretation method that is frequently used with magnetic and gravity data. For a given source type, which is specified by its structural index (SI), it provides an estimate of the source location. It is demonstrated here that by computing the solution space of individual data points and selecting common source locations the accuracy of the result can be improved. Furthermore, only a slight modification of the method is necessary to allow solutions for any number of different SIs to be obtained simultaneously. The method is applicable to both evenly and unevenly sampled geophysical data and is demonstrated on gravity and magnetic data. Source code (in Matlab format) is available from www.iamg.org. | G R J Cooper | 2008 | Journal of China University of Geosciences2008，19，1： | 3 |
| 4 | Inflammation and Alzheimer’s disease显示文摘 | Haruhiko Akiyama Steven Barger Scott Barnum Bonnie Bradt Joachim Bauer Greg M Cole Neil R Cooper Piet Eikelenboom Mark Emmerling Berndt L Fiebich Caleb E Finch Sally Frautschy W.S.T Griffin Harald Hampel Michael Hull Gary Landreth Lih–Fen Lue Robert Mrak | 2000 | Neurobiology of Aging2000，，3： | 2 |
| 5 | Load regulation of the properties of adult feline cardiocytes: growth induction by cellular deformation显示文摘 | Mann D L Kent R L Cooper G | 1989 | Circ Res1989，64，6： | 2 |
| 6 | Some models for estimating technical and scale inefficiency in data envelopment analysis显示文摘 | BANKER R D CHARMES A COOPER W W | 1984 | Management Science1984，32，： | 2 |
| 7 | The safety of tenofovir disoproxil fumarate for the treatment of HIV infection in adults: the first 4 years显示文摘 | Mark R Nelson Christine Katlama Julio S Montaner David A Cooper Brian Gazzard Bonaventura Clotet Adriano Lazzarin Knud Schewe Joep Lange Christina Wyatt Sue Curtis Shan-Shan Chen Stephen Smith Norbert Bischofberger James F Rooney | 2007 | AIDS2007，，10： | 2 |
| 8 | 牙本质胞外基质对人血管内皮细胞增殖的影响显示文摘目的 :探讨牙本质胞外基质成分 (dentineextracellularmatrixcomponents,DM)对血管内皮细胞增殖的影响。方法 :用 10 0 g/LEDTA ,在pH 7.2和蛋白酶抑制剂存在的条件下 ,从健康人牙中提取DM。第 3代人脐静脉内皮细胞 (humanumbilicalveinendothelialcell,HUVEC) ,在含有不同浓度DM培养液中培养 8d ,每 2天计数 1次 ,绘制生长曲线。并对第 8天对照 (不含DM培养 )与实验组的细胞数进行Mann -Whitney统计分析。结果 :HUVEC在DM浓度为 0 .0 0 0 1、0 .0 0 1和 0 .0 1mg/mL的培养液中培养时较对照分别增长了 4 9% (P =0 .0 0 0 6 )、2 1%和 9% ,在DM浓度为 0 .1和 1mg/mL时 ,减少 2 2 %和 74 % (P =0 .0 0 0 3)。 结论 :DM对血管内皮细胞增殖具有计量依赖型影响效应。低浓度DM可刺激内皮细胞的增殖 ,而高浓度DM则可抑制该细胞的增殖。提示 :这一计量依赖型效应可能正是牙髓损伤后造成局部不同的血管形成方式 ,并最终导致不同修复结果的原因。 | 张蓉 Gay Smith Alastair J Sloan Paul R Cooper 赵守亮 Athony J Smith | 2004 | 牙体牙髓牙周病学杂志2004，14，6： | 2 |
| 9 | Nitric oxide synthases: structure, function and inhibition显示文摘 | Alderton W K Cooper C E Knowles R G | 2001 | Biochem J2001，357，3： | 2 |
| 10 | Plasmid-mediated romet resistance of Edwardsiella ictaluri 显示文摘 | STARLIPER C E COOPER R K SHOTrS E B | 1993 | Journal of Aquatic Animal Health1993，5，： | 1 |
| 11 | Cuticle-degrading enzymes of entomopathogenic fungi :Regulation of productionof chitinolytic enzymes显示文摘 | STLEGER RJ COOPER R M CHARNLEY A K | 1986 | Gen Microbio1986，132，： | 1 |
| 12 | Response of soybean cultivars to narrow row sand,planting rates under Weed-free conditions显示文摘 | Cooper R L | 1977 | Agronomy Journal1977，69，1： | 1 |
| 13 | The treatment of wastewater from the leather industry显示文摘 | COOPER D R SHUTTLEWORTH S G SLABBERT N P | 1984 | Water PoUution Control1984，83，4： | 1 |
| 14 | The efficacy and safety of B-cell depletion with anti-CD20 monoclonal antibody in adults with chronic immune thrombocytopenic purpura显示文摘 | COOPER N STASI R CUNNINGHAM-RUNDLES S | 2004 | Br J Haematol2004，125，： | 1 |
| 15 | Attenuation,persistence,and vaccine potential of an Edwardsiella ictaluri purA mutant显示文摘 | Lawrence M L Cooper R K Thune R L | 1997 | Infection and Immunity1997，65，11： | 1 |
| 16 | Manipulation of the n-3 PUFA content of muscle and adipose tissue in lambs显示文摘 | Cooper S L Sinclair L A Wilkinson R G | 2004 | J Animal Sci2004，82，： | 1 |
| 17 | Design of ultra-low-cost UHF RFID tags for supply chain applications显示文摘 | Glidden R Bockorick C Cooper S | 2004 | IEEE Communications Magazine2004，42，8： | 1 |
| 18 | Advances in electrostatic crop spraying of conductive pesticides 显示文摘 | Law S E Cooper S C Oetting R D | 1992 | Transactions of ASAE1992，11，4： | 1 |
| 19 | Some Models for Estimating Technical and Scale Inefficiencies in Data Envelopment Analysis显示文摘 | Banker R D Cooper W W | 1984 | Management Science1984，9，30： | 1 |
| 20 | Information technology implementation research: A technological diffusion approach 显示文摘 | Cooper R B Zmud R W | 1990 | Management Science1990，36，2： | 1 |
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