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| 1 | Immunohistochemical expression of SP-NK-1R-EGFR pathway and VDR in colonic inflammation and neoplasia显示文摘AIM:To determine the expression of neurokinin-1receptor(NK-1R),phosphorylated epidermal growth factor receptor(p EGFR),cyclooxygenase-2(Cox-2),and vitamin D receptor(VDR)in normal,inflammatory bowel disease(IBD),and colorectal neoplasia tissues from Puerto Ricans.METHODS:Tissues from patients with IBD,colitisassociated colorectal cancer(CAC),sporadic dysplasia,and sporadic colorectal cancer(CRC),as well as normal controls,were identified at several centers in Puerto Rico.Archival formalin-fixed,paraffin-embedded tissues were de-identified and processed by immunohistochemistry for NK-1R,p EGFR,Cox-2,and VDR.Pictures of representative areas of each tissues diagnosis were taken and scored by three observers using a4-point scale that assessed intensity of staining.Tissues with CAC were further analyzed by photographing representative areas of IBD and the different grades of dysplasia,in addition to the areas of cancer,within each tissue.Differences in the average age between the five patient groups were assessed with one-way analysis of variance and Tukey-Kramer multiple comparisons test.The mean scores for normal tissues and tissues with IBD,dysplasia,CRC,and CAC were calculatedand statistically compared using one-way analysis of variance and Dunnett’s multiple comparisons test.Correlations between protein expression patterns were analyzed with the Pearson’s product-moment correlation coefficient.Data are presented as mean±SE.RESULTS:On average,patients with IBD were younger(34.60±5.81)than normal(63.20±6.13,P<0.01),sporadic dysplasia(68.80±4.42,P<0.01),sporadic cancer(74.80±4.91,P<0.001),and CAC(57.50±5.11,P<0.05)patients.NK-1R in cancer tissue(sporadic CRC,1.73±0.34;CAC,1.57±0.53)and sporadic dysplasia(2.00±0.45)were higher than in normal tissues(0.73±0.19).p EGFR was significantly increased in sporadic CRC(1.53±0.43)and CAC(2.25±0.47)when compared to normal tissue(0.07±0.25,P<0.05,P<0.001,respectively).Cox-2 was significantly increased in sporadic colorectal cancer(2.20±0.23 vs 0.80±0.37 for normal tissues,P<0.05).In comparison to normal(2.80±0.13)and CAC(2.50±0.33)tissues,VDR was significantly decreased in sporadic dysplasia(0.00±0.00,P<0.001 vs normal,P<0.001 vs CAC)and sporadic CRC(0.47±0.23,P<0.001 vs normal,P<0.001 vs CAC).VDR levels negatively correlated with NK-1R(r=-0.48)and p EGFR(r=-0.56)in normal,IBD,sporadic dysplasia and sporadic CRC tissue,but not in CAC.CONCLUSION:Immunohistochemical NK-1R and p EGFR positivity with VDR negativity can be used to identify areas of sporadic colorectal neoplasia.VDR immunoreactivity can distinguish CAC from sporadic cancer. | Raymond A Isidro Myrella L Cruz Angel A Isidro Axel Baez Axel Arroyo William A González-Marqués Carmen González-Keelan Esther A Torres Caroline B Appleyard | 2015 | World Journal of Gastroenterology2015，21，6： | 7 |
| 2 | Serum vitamin D and colonic vitamin D receptor in inflammatory bowel disease显示文摘AIM: To determine serum vitamin D levels and colonic vitamin D receptor(VDR) expression in inflammatory bowel disease(IBD) and non-IBD patients and correlate these with histopathology.METHODS: Puerto Rican IBD(n = 10) and non-IBD(n = 10) patients ≥ 21 years old scheduled for colonoscopy were recruited. Each patient completed a questionnaire and provided a serum sample and a colonic biopsy of normal-appearing mucosa. For IBD patients, an additional biopsy was collected from visually diseased mucosa. Serum vitamin D levels were measured by ultra-performance liquid chromatography and mass spectrometry. Hematoxylin and eosin stained tissue sections from colonic biopsies were classified histologically as normal or colitis(active/inactive), and scored for the degree of inflammation present(0-3, inactive/absent to severe). Tissue sections from colonic biopsies were also stained by immunohistochemistry for VDR, for which representative diagnostic areas were photographed and scored for staining intensity using a 4-point scale.RESULTS: The IBD cohort was significantly younger(40.40 ± 5.27, P < 0.05) than the non-IBD cohort(56.70 ± 1.64) with a higher prevalence of vitamin D deficiency(40% vs 20%, respectively) and insufficiency(70% vs 50%, respectively). Histologic inflammation was significantly higher in visually diseased mucosa from IBD patients(1.95 ± 0.25) than in normalappearing mucosa from control patients(0.25 ± 0.08, P < 0.01) and from IBD patients(0.65 ± 0.36, P < 0.05) and correlated inversely with VDR expression in visually diseased colonic tissue from IBD patients(r =-0.44, P < 0.05) and from IBD patients with Crohn's disease(r =-0.69, P < 0.05), but not in normal-appearing colonic tissue from control patients or IBD patients. Control and IBD patient serum vitamin D levels correlated positively with VDR expression in normal colon from control and IBD patients(r = 0.38, P < 0.05) and with patient age(r = 0.54, P < 0.01). CONCLUSION: Levels of serum vitamin D correlate positively with colonic VDR expression in visually normal mucosa whereas inflammation correlates negatively with colonic VDR expression in visually diseased mucosa in Puerto Rican patients. | Yamilka Abreu-Delgado Raymond A Isidro Esther A Torres Alexandra González Myrella L Cruz Angel A Isidro Carmen I González-Keelan Priscilla Medero Caroline B Appleyard | 2016 | World Journal of Gastroenterology2016，22，13： | 5 |
| 3 | Control of malaria and other vector-borne protozoan diseases in the tropics: enduring challenges despite considerable progress and achievements显示文摘Vector-borne protozoan diseases represent a serious public health challenge,especially in the tropics where poverty together with vector-favorable climates are the aggravating factors.Each of the various strategies currently employed to face these scourges is seriously inadequate.Despite enormous efforts,vaccines-which represent the ideal weapon against these parasitic diseases—are yet to be sufficiently developed and implemented.Chemotherapy and vector control are therefore the sole effective attempts to minimize the disease burden.Nowadays,both strategies are also highly challenged by the phenomenon of drug and insecticide resistance,which affects virtually all interventions currently used.The recently growing support from international organizations and governments of some endemic countries is warmly welcome,and should be optimally exploited in the various approaches to drug and insecticide research and development to overcome the burden of these prevalent diseases,especially malaria,leishmaniasis,Human African Trypanosomiasis(HAT),and Chagas disease. | Denis Zofou Raymond B Nyasa Dickson S Nsagha Fidele Ntie-Kang Henry D Meriki Jules Clement N Assob Victor Kuete | 2014 | Infectious Diseases of Poverty2014，3，1： | 4 |
| 4 | Assessing the impacts of climate change on water resources of a West African trans-boundary river basin and its environmental consequences (Senegal River Basin)显示文摘The Senegal River Basin (SRB) is a shared watershed in West Africa which includes regions (the upper basin, valley, and delta), characterized by distinct environmental conditions. An important feature of the Senegal River flow volume historically was its inter-annual irregularity, which caused a major water resource constrain. This situation has been accentuated during the long-term drought (1969-1984) in the Sahel zone which highlights the vulnerability of food-producing systems to climate change and variability. SRB is undergoing fundamental environmental, hydrologic, and socioeconomic transitions and represents a good illustration of sensitivity to climatic variations and opportunities for adaptation. This paper aims to study water resources systems under stress from climate variability and change in the Senegal River Basin. The results show (1) through the compilation of available data, information and knowledge (sedimentological, climatical, geological, environmental, archeological, etc.), the chronological consequences of climate change during the past millennium in West Africa, and also (2) an analysis of the recent impacts and vulnerability to climate change in the SRB and finally (3) the adaptation strategies in the SRB in order to identify and resolve problems associated with this water scarcity and to address the potential for guaranteed agricultural production in this narrow geographic area. | Cheikh Bécaye Gaye Moctar Diaw Raymond Malou | 2013 | Research in Cold and Arid Regions2013，5，1： | 2 |
| 5 | Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level显示文摘Mesenchymal stem cells(MSCs)have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions,including the paracrine effect,cell fusion,and mechanical and electrical couplings.Due to heart–muscle complexity,progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture.In conventional coculture systems,however,the in vivo cardiac muscle structure,in which rod-shaped cells are connected end-to-end,is not sustained;instead,irregularly shaped cells spread randomly,resulting in randomly distributed cell junctions.Consequently,contact-mediated cell–cell interactions(e.g.,the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions)occur randomly.Thus,the data generated on the beneficial effects of MSCs may be irrelevant to in vivo biological processes.In this study,we explored whether cardiomyocyte alignment,the most important phenotype,is relevant to stem cell cardiogenic differentiation.Here,we report(i)the construction of a laser-patterned,biochip-based,stem cell–cardiomyocyte coculture model with controlled cell alignment;and(ii)single-cell-level data on stem cell cardiogenic differentiation under in vivo-like cardiomyocyte alignment conditions. | Zhen Ma Qiuying Liu Huaxiao Yang Raymond B Runyan Carol A Eisenberg Meifeng Xu Thomas K Borg Roger Markwald Yifei Wang Bruce Z Gao | 2013 | Light(Science & Applications)2013，2，1： | 2 |
| 6 | Combined acid deamidation and enzymic hydrolysis for improvement of the functional properties of wheat gluten 显示文摘 | MIMOUNI B RAYMOND J MERLE - DESNOYERS A M | 1994 | J Cereal Sci1994，21，2： | 1 |
| 7 | Pharmacotherapeutic options for the treatment of depression in patients with chronic kidney disease显示文摘 | RAYMOND C B WAZNY L D HONCHARIK P L | 2008 | Nephrol Nurs J2008，35，26： | 1 |
| 8 | Blockade of CD40 - CD40 ligand Interactions protects against radiation induced pulmonary inflammation and fibrosis 显示文摘 | Adnan A Ying Z Raymond B | 1998 | Clin Immunol Immunopathol1998，89，3： | 1 |
| 9 | Galant hamine derivatives for the treat ment of Alzheimer's disease显示文摘 | Raymond W K | 1996 | Drugs Fut1996，21，： | 1 |
| 10 | Evaluation of The Dispersional Analysis Method for Fractal Time Series显示文摘 | Bassingthwaighte Gary M Raymond | 1995 | Annals of Biomedical Engineering1995，，： | 1 |
| 11 | Cloning and analysis of MAGE-1 related genes 显示文摘 | Min D Raymond J B Christopher J | 1994 | Biochem Biophys Res Commun1994，202，1： | 1 |
| 12 | Aggregates of scrapie-associated prion protcin induce the cell-free conversion of protease-sentitive prion protein to the protease-resistant state显示文摘 | Caughey B Kocisko D A Raymond G J | 1995 | Chem Biol1995，2，： | 1 |
| 13 | Cloning and functional expression of a cDNA encoding geranylgeranyl diphosphate synthase from Taxus canadensis and assessment of the role of this prenyhransferase in cells induced for taxol production 显示文摘 | Jerry Hefner Raymond E B Ketchum Rodney Croteau | 1998 | Arch Biochem Biophys1998，360，： | 1 |
| 14 | Temporal variability of aphid biological control in contrasting landscape contexts显示文摘 | Raymond L Ortiz-Martinez S A Lavandero B | 2015 | Biological Control2015，90，： | 1 |
| 15 | Deriving Dispersional and Scaled Windowed Variance Analyses Using The Correlation Function of Discrete Fractional Gaussian Noise显示文摘 | James B Bassingthwaighte | 1999 | Physica A1999，265，： | 1 |
| 16 | Anthraquinones as a Aew Class of Antiviral Agents Against Human Immunodeficiency Virus显示文摘 | Raymond F S Chuay K CJ Ramesh B | 1990 | Antiviral Research1990，13，： | 1 |
| 17 | Evolution of Resistance to High Temperature in Ectotherms显示文摘 | Raymond B Huey Joel G K | 1993 | The American Naturalist1993，142，： | 1 |
| 18 | Charge Control:Modeling,Analysis,and Design显示文摘 | Wei Tang Fred C Lee Raymond B Ridley Isaac Cohen | 2003 | IEEE Transactions on Power Electronics2003，，10： | 1 |
| 19 | A murine model of experimentalmetastasis to bone and bone marrow显示文摘 | Francisco ARGUELLO Raymond B BAGGS FRANTZChristopher N | 1988 | Cancer Res1988，48，23： | 1 |
| 20 | Genetic and biochemical characterization of field-evolved resistance to Bacillus thuringiensis toxin CrylAc in the diamondback moth,Plutella xylostella显示文摘 | SAYYED A H RAYMOND B IBIZA-PALACIOS M S | 2004 | Appl Environ Microbiol2004，70，12： | 1 |
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