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| 1 | Synthesis of functional polycarbonates by lipase-catalyzed ring-opening polymerization 显示文摘 | Feng Jun Zhuo Renxi He Feng | 2003 | Macromol Symp2003，195，1： | 1 |
| 2 | Polymerization of trimethylene carbonate with high molecular weight catalyzed by immobilized lipase on silica microparticles 显示文摘 | Feng Jun He Feng Zhuo Renxi | 2002 | Macromolecules2002，35，19： | 1 |
| 3 | Reflective ultrathin light-sheet microscopy with isotropic 3D resolutions显示文摘Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging capabilities.The perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their isotropy.Here,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path reflector.The conventional high NA objectives can be used both in excitation and detection with this design.Isotropic resolutions in 3D down to300 nm could be achieved without deconvolution.The proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy. | YUE WANG DASHAN DONG WENKAI YANG RENXI HE MING LEI KEBIN SHI | 2024 | Photonics Research2024，12，2： | 0 |
| 4 | PN-ImTLSM facilitates high-throughput low background single-molecule localization microscopy deep in the cell显示文摘When imaging the nucleus structure of a cell,the out-of-focus fluorescence acts as background and hinders the detection of weak signals.Light-sheet fluorescence microscopy(LSFM)is a wide-field imaging approach which has the best of both background removal and imaging speed.However,the commonly adopted orthogonal excitation/detection scheme is hard to be applied to single-cell imaging due to steric hindrance.For LSFMs capable of high spatiotemporal single-cell imaging,the complex instrument design and operation largely limit their throughput of data collection.Here,we propose an approach for high-throughput background-free fluorescence imaging of single cells facilitated by the Im-mersion Tilted Light Sheet Microscopy(ImTLSM).ImTLSM is based on a light-sheet projected off the optical axis of a water immersion objective.With the illumination objective and the detection objective placed opposingly,ImTLSM can rapidly patrol and optically section multiple individual cells while maintaining single-molecule detection sensitivity and resolution.Further,the simplicity and robustness of ImTLSM in operation and maintenance enables high-throughput image collection to establish background removal datasets for deep learning.Using a deep learning model to train the mapping from epi-illumination images to ImTLSM illumination images,namely PN-ImTLSM,we demonstrated crossmodality fluorescence imaging,transforming the epi-illumination image to approach the background removal performance obtained with ImTLSM.We demonstrated that PN-ImTLSM can be generalized to large-field homogeneous illumination imaging,thereby further improving the imaging throughput.In addition,compared to commonly used background removal methods,PN-ImTLSM showed much better performance for areas where the background intensity changes sharply in space,facilitating high-density single-molecule localization microscopy.In summary,PN-ImTLSM paves the way for background-free fluorescence imaging on ordinary inverted microscopes. | Boxin Xue Caiwei Zhou YizhiQin Yongzheng Li Yuao Sun Lei Chang Shipeng Shao Yongliang Li Mengling Zhang Chaoying Sun Renxi He Qian Peter Su Yujie Sun | 2021 | Biophysics Reports2021，7，4： | 0 |
| 5 | Redox-Responsive Molecular Gels Based on Camptothecin Prodrug with Disulfide Linkage for Controlled and Sustained Drug Release显示文摘A novel camptothecin(CPT) prodrug was successfully synthesized by conjugating CPT to adamantanecarboxylic acid(AD) via a cleavable disulfide linkage. The resulting CPT-ss-AD prodrug could act as a low molecular weight gelator to form molecular gels in water/water-miscible organic solvent mixture. Meanwhile, biodegradable amphiphilic block copolymer m PEG-b-P(MAC-co-DTC)(PPMD) was also employed as an organic framework together with CPT-ss-AD to form gel structure. CPT-ss-AD/PPMD gel exhibited less compact molecular arrangement but much more stability than CPT-ss-AD gel. The two kinds of gels could effectively release the original CPT under reductive condition at a near-constant rate without any initial burst. As compared to CPT-ss-AD single-component gel, the two-component gel, CPT-ss-AD/PPMD, had a significantly higher release rate of CPT, while 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide(MTT) assays also indicated highly potent cytotoxic activity against He La cells. | CHANG Xiupeng LI Youmei CHEN Shu HE Feng ZHUO Renxi | 2017 | Wuhan University Journal of Natural Sciences2017，22，5： | 0 |
| 6 | Study on Synthesis and Herbicidal Activity of Heterocyclic Compounds Containing P-P Bond显示文摘N,N’-diphenylurea reacted with phosphorus trichloride and phenyl dicklorophospbane giving theheterocyclic compounds 1 and 2 with a direct phosphorus-phosphorus bond (P-P bond ), respectively. The newcompounds were characterized by elementary analysis, NMR and IR spectra. The results or preliminary bioassay showed that these heterocycles possess selective herbicidal activity at 1.5 kg/hm2. | He Liangnian Zhuo Renxi Cai Lei Lu Alhong | 1998 | Wuhan University Journal of Natural Sciences1998，3，1： | 0 |
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