|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Using induced pluripotent stem cells as a tool for modelling carcinogenesis显示文摘Cancer is a highly heterogeneous group of diseases that despite improved treatments remain prevalent accounting for over 14 million new cases and 8.2 million deaths per year. Studies into the process of carcinogenesis are limited by lack of appropriate models for the development and pathogenesis of the disease based on human tissues. Primary culture of patient samples can help but is difficult to grow for a number of tissues. A potential opportunity to overcome these barriers is based on the landmark study by Yamanaka which demonstrated the ability of four factors;Oct4, Sox2, Klf4, and c-Myc to reprogram human somatic cells in to pluripotency. These cells were termed induced pluripotent stem cells(i PSCs) and display characteristic properties of embryonic stem cells. This technique has a wide range of potential uses including disease modelling, drug testing and transplantation studies. Interestingly i PSCs also share a number of characteristics with cancer cells including self-renewal and proliferation, expression of stem cell markers and altered metabolism. Recently, i PSCs have been generated from a number of human cancer cell lines and primary tumour samples from a range of cancers in an attempt to recapitulate the development of cancer and interrogate the underlying mechanisms involved. This review will outline the similarities between the reprogramming process and carcinogenesis, and how these similarities have been exploited to generate i PSC models for a number of cancers. | Emma L Curry Mohammad Moad Craig N Robson Rakesh Heer | 2015 | World Journal of Stem Cells2015，7，2： | 5 |
| 2 | Reduced semen quality in patients with testicular cancer seminoma is associated with alterations in the expression of sperm proteins显示文摘Testicular cancer seminoma is one of the most common types of cancer among men of reproductive age.Patients with this condition usually present reduced semen quality,even before initiating cancer therapy.However,the underlying mechanisms by which testicular cancer seminoma affects male fertility are largely unknown.The aim of this study was to investigate alterations in the sperm proteome of men with seminoma undergoing sperm banking before starting cancer therapy,in comparison to healthy proven fertile men(control group).A routine semen analysis was conducted before cryopreservation of the samples(n=15 per group).Men with seminoma showed a decrease in sperm motility(P=0.019),total motile count(P=0.001),concentration(P=0.003),and total sperm count(P=0.001).Quantitative proteomic analysis identified 393 differentially expressed proteins between the study groups.Ten proteins involved in spermatogenesis,sperm function,binding of sperm to the oocyte,and fertilization were selected for validation by western blot.We confirmed the underexpression of heat shock-related 70 kDa protein 2(P=0.041),ubiquinol-cytochrome C reductase core protein 2(P=0.026),and testis-specific sodium/potassium-transporting ATPase subunit alpha-4(P=0.016),as well as the overexpression of angiotensin I converting enzyme(P=0.005)in the seminoma group.The altered expression levels of these proteins are associated with spermatogenesis dysfunction,reduced sperm kinematics and motility,failure in capacitation and fertilization.The findings of this study may explain the decrease in the fertilizing ability of men with seminoma before starting cancer therapy. | Tania R Dias Ashok Agarwal Peter N Pushparaj Gulfam Ahmad Rakesh Sharma | 2020 | Asian Journal of Andrology2020，22，1： | 2 |
| 3 | Genistein induces apoptosis in ovarian cancer cells via different molecular pathways depending on Breast Cancer Susceptibility gene-1 (BRCA1)status显示文摘 | THASNI K A ROJINI G RAKESH S N | 2008 | Eur J Pharmacol2008，588，2： | 1 |
| 4 | M3-Deterministic multiscale multirobot Platform for Microsystems packaging显示文摘 | Popa Dan O Murthy Rakesh Das Aditya N | 2009 | IEEE Transactions on Automation Science and Engineering2009，6，2： | 1 |
| 5 | Predictive power for life and residual life of the zinc rich primer coatings with electrical measurement显示文摘 | R N Jagtap Rakesh Nambiar S Zaffar Hassan | 2007 | Progress in Organic Coatings2007，58，： | 1 |
| 6 | Data Mining in an Engineering Design Environment; OR Applications from Graph Matching 显示文摘 | Carol J R Rakesh N Moises S | 2006 | Computers & Operations Research2006，，： | 1 |
| 7 | Automated Retrieval and Ranking of Similar Parts in Agile Manufacturing 显示文摘 | Shekhar I Rakesh N | 1997 | IIE Transactions1997，29，： | 1 |
| 8 | Customer profitability in a supply chain显示文摘 | Rakesh N Mahendra G Chakravarthi N | 2001 | Journal of Marketing2001，，65： | 1 |
| 9 | Analysis of congenital hypomyelinating Egr2Lo/Lo nerves identifies Sox2 as an inhibitor of Schwann cell differentiation and myelination显示文摘 | Nam L Rakesh N James YT | 2005 | Neurosci2005，102，7： | 1 |
| 10 | Customer profitability in a supply chain显示文摘 | Rakesh N Mahendra G Chakravarthi N | 2001 | Journal of Marketing2001，，65： | 1 |
| 11 | A data mining approach to form- ing generic bills of materials in support of variant design activities 显示文摘 | Romanowski Carol J Rakesh N | 2004 | Journal of Computing and Information Science in Engi- neering2004，12，4： | 1 |
| 12 | Seismically induced soft-sediment deformation structures(seismites) around Sumdo in the Lower Spitti valley(Tethys Himalaya)显示文摘 | Rakesh Mohindra Bagati T N | 1996 | Sedmentary Geology1996，101，12： | 1 |
| 13 | Strategic capacity planning in supply chain design for a new market opportunity显示文摘 | Satyaveer S C Rakesh N Proth J M | 2004 | International Journal of Production Research2004，42，11： | 1 |
| 14 | Robust supply chain design under uncertain demand in agile manufacturing显示文摘 | Feng P Rakesh N | | 0，，04： | 1 |
| 15 | Some novel attributes of graphene 显示文摘 | Rao C N R Sood A K Rakesh V | 2010 | The Journal of Physical Chemistry Letters2010，1，2： | 1 |
| 16 | Changes in gene expression in liver tissue from patients with fulminant hepatitis E显示文摘AIM: To study host gene expression and numberof immune cells in liver tissues from patients with fulminant hepatitis E(FH-E).METHODS: Microarray-based expression profiling was done using Illumina Human WG-6_v3_Bead Chip arrays on post-mortem liver tissue from 5 patients with FH-E,and compared with similar tissue from 6 patients with fulminant hepatitis B(FH-B; disease controls) and normal liver tissue from 6 persons.Differential expression was defined as ≥ 2.0-fold change with Benjamini-Hochberg false discovery rate below 0.05 using t-test in liver tissue from FH-B and FH-E,than healthy liver tissue.For some genes that showed differential expression in FH-E,microarray data were validated using quantitative reverse transcription PCR.Differentially expressed gene lists were then subjected to 'Gene Ontology' analysis for biological processes,and pathway analysis using Bio Carta database on the DAVID server.In addition,tissue sections were stained for CD4+,CD8+ and CD56+ cells using indirect immunohistochemistry; cells staining positive for each of these markers were counted and compared between groups.RESULTS: Compared to normal livers,those from patients with FH-E and FH-B showed differential expression of 3377 entities(up-regulated 1703,downregulated 1674) and 2572 entities(up 1164,down 1408),respectively.This included 2142(up 896,down 1246) entities that were common between the two sets; most of these belonged to metabolic,hemostatic and complement pathways,which are active in normal livers.Gene expression data from livers of patients with FH-E but not those of FH-B showed activation of several immune response pathways,particularly those involving cytotoxic T cells.The fold-change values of m RNA for selected genes in livers from FH-E than in normal liver tissue determined using quantitative reverse transcription PCR showed excellent concordance with microarray analysis.At immunohistochemistry,CD8+ T cells showed an increase in liver biopsies from both FH-E [median 53.4 per arbitrary unit area(range 31.2-99.9)] and FH-B [median 49.3(19.3-51.0); P = 0.005] compared to control liver tissue [median 6.9(3.1-14.9)].CONCLUSION: FH-E patients show CD8+ T cell infiltration and increased gene expression of cytotoxic T cell pathways in liver,suggesting a possible pathogenetic role for these cells. | Anshu Naik Amit Goel Vinita Agrawal Aditya N Sarangi Nanda Chhavi Vineeta Singh Shahid Jameel Rakesh Aggarwal | 2015 | World Journal of Gastroenterology2015，21，26： | 1 |
| 17 | Artificial neural networks--genetic algorithm based model for back calculation of pavement layer moduli 显示文摘 | RAKESH N JAIN A K REDDY M A REDDY K S | 2006 | International Journal of Pavement Engineering2006，7，3： | 1 |
| 18 | STEP-based feature extraction from STEP geometry for Agile manufacturing显示文摘 | RAKESH N | 2000 | Computers in industry2000，41，： | 1 |
| 19 | Relationship between femur bone mineral density, body mass index and dental panoramic mandib- ular cortical width in diagnosis of elderly postmenopausal women with osteoporosis显示文摘 | Nagi R Devi BK RaKesh N | 2014 | J Clin Diagn Res2014，8，8： | 1 |
| 20 | Automated retrieval and ranking of similar parts in agile manufacturing显示文摘 | SHEKHAR I RAKESH N | 1997 | IIE Transaction1997，29，10： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员