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| 1 | Involvement of auxin in growth and stress response of cucumber显示文摘Cucumber(Cucumis sativus L.)is an important vegetable,popular worldwide for its crispy texture and special flavor.Plant hormones such as auxin stand out for its dominating function in morpho-and organogenic processes,formation of organs as well as regulation of tropic responses.These developmental processes are entirely,or partially dependent on auxin biosynthesis,transport,and signal transduction.In cucumber,auxin not only fine-tunes its morphogenesis but also its response to environmental stress.The role of auxin in regulating different organs(root,hypocotyl,shoot,leaf,tendril,flower,and fruit)development in cucumber is reviewed in the present paper.Moreover,the role of auxin in cucumber response to biotic stresses(powdery mildew,downy mildew,and nematode infections)and abiotic stresses(heat,iron,waterlogging,cold,salinity,drought,and heavy metal stresses)is discussed.Finally,we point out the blind spots and future research thoughts to extend our understanding of this myriad molecule in cucumber growth and stress biology. | Rahat Sharif Li Su Xuehao Chen Xiaohua Qi | 2022 | Vegetable Research2022，2，1： | 1 |
| 2 | A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype显示文摘Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis,photosynthesis mechanism and chloroplast development.Herein,we identified a spontaneous mutant,yc412,in cultivated cucumber that exhibited yellow cotyledons.The yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure,and reduced photosynthetic capacity and pigment content.Through bulked segregant analysis-based whole-genome sequencing and fine genetic mapping,we narrowed the yellow cotyledons (yc) locus to a 96.8 kb interval on chromosome 3.By resequencing and molecular cloning,we showed that Csyc is a potential candidate gene,which encodes a yellow stripe-like (YSL) transporter.The T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in yc412.Compared to YZU027A (WT),the expression of Csyc was significantly downregulated in the cotyledons of yc412.Silencing of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves,mainly by suppressing the chlorophyll content.Furthermore,a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 cotyledons.Our results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis. | Jiawei Pan Jia Song Rahat Sharif Xuewen Xu Shutong Li Xuehao Chen | 2024 | Journal of Integrative Agriculture2024，23，3： | 0 |
| 3 | Study of JASMONATE ZIM-Domain gene family to waterlogging stress in Cucumis sativus L.显示文摘Jasmonate ZIM Domain proteins(JAZs)provide a response to multiple abiotic stresses by critically regulating the JA signaling pathway.In this study,a total of 11 CsJAZ genes were identified in the cucumber genome database.In silico analysis revealed the presence of different cis-acting regulatory elements related to growth,stress and hormonal responsiveness in the upstream region of CsJAZ genes.The transcriptional expression pattern analysis showed that all CsJAZ genes were expressed in the tissues tested and all CsJAZ genes responded to plant hormone GA,suggesting they may participate in complex hormone signaling networks.An expression analysis based on qRT-PCR,indicated that cucumber CsJAZ8 was decreased significantly by waterlogging treatment but induced by exogenous methyl jasmonate application.Therefore,we speculate that CsJAZ8 could be a potential regulator of the response of cucumber to waterlogging stress.Additionally,transient expression assay showed that the CsJAZ8 protein is localized in the nucleus and could be involved in the regulation of various biological and cellular processes.Furthermore,Y2H assay revealed that cucumber CsJAZ proteins exhibit homologous and heterologous interactions.Subsequently,this research will contribute to the understanding of the CsJAZ gene family in Cucumis sativus.It also provides further insight into the role of CsJAZ proteins in waterlogging stress and may provide a new frontier in cucumber breeding for waterlogging tolerance. | Jiawei Pan Jingyun Tu Rahat Sharif Xiaohua Qi Xuewen Xu Xuehao Chen | 2021 | Vegetable Research2021，1，1： | 0 |
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