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2篇 您的检索式:作者名="Rebaone Letsididi"
    题名 作者 年代 出处 被引量
1产环糊精葡萄糖基转移酶菌株的筛选、鉴定与应用研究显示文摘利用产环糊精葡萄糖基转移酶(CGTase)菌株快速筛选法结合芦丁转糖基酶反应,筛选获得一株所产CGTase可高效转化芦丁的菌株。16SrRNA鉴定表明,此菌株和地衣芽孢杆菌(Bacillus licheniformis)有100%的同源性。结合形态和生理生化鉴定,确定此菌株为地衣芽孢杆菌,命名为Bacillus licheniformis SK13.002。该菌株在pH约为10时生长良好,且具有最高的产酶量,显示其嗜碱性。此菌株所产酶以淀粉作底物所产环糊精为β-环糊精和γ-环糊精,其中β-环糊精所占比例为83.3%;两者产量分别达到20.9g/L和4.2g/L。直接利用浓缩酶液进行芦丁的糖基化,则可以得到近70%的转化率。孙涛 江波 潘蓓蕾 Rebaone Letsididi 2010食品工业科技2010,31,10:2
2Influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits显示文摘Objective:To evaluate the influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R.Br.ex Sabine fruits.Methods:The antibacterial activities of pulp and seed extracts were tested by using disk diffusion method against eight bacterial strains and three fungal strains.Total phenolic,flavonoid,monomeric anthocyanin and tannin contents,and antioxidant activities were determined by spectrometric methods.Results:The antioxidant analysis of pulp extract revealed the strong radical scavenging capacity and total phenolic content(4 329.66 mg of gallic acid/100 g),while seed extract showed the high antioxidant activity and total tannin content(5 326.78 mg catechin equivalent/100 g).Antibacterial and antifungal activities of methanol and ethanol extracts exhibited potent growth inhibitory activity against Aeromonas hydrophila,Bacillus subtilis,Escherichia coli O157:H7,Pseudomonas aeruginosa,Salmonella Typhimurium and Staphylococcus aureus ATCC 6538 with minimum inhibitory concentration values ranged from 125 to 250 μg/mL.However,seed extract had the strongest potential activity against Aspergillus niger and Candida albicans with minimum inhibitory concentration value of 500 μg/mL compared to pulp extract.Conclusions:Our results therefore demonstrated that ethanol and methanol extractions were more efficient in extracting antioxidants and bioactive compound in pulp and seed.These results support that these plant extracts can be used for the treatment of bacterial infections.Gbago Onivogui Rebaone Letsididi Mohamed Diaby Liping Wang Yuanda Song 2016Asian Pacific Journal of Tropical Biomedicine2016,6,1:0
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