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| 1 | Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer显示文摘Colorectal cancer(CRC)is the second leading cause of cancer-related death worldwide.The five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced stages.Autoantibodies against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early diagnosis.Thus,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid biopsy.To that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to CRC.After bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC prognosis.After validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC detection.Taken together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here could be integrated into a point-of-care(POC)device for CRC detection with high diagnostic ability. | Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma Domínguez Ana Guzman-Aranguez Alberto Peláez-García JoséManuel Pingarrón Susana Campuzano Rodrigo Barderas | 2021 | Engineering2021，7，10： | 1 |
| 2 | Structure of the cross-β spine of amyloid-like fibrils显示文摘 | David Eisenberg Rebeca Nelson Michael R Sawaya | 2005 | Nature2005，435，： | 1 |
| 3 | Inheritance of tomato late blight resistance derived from Lycopersicon hirsutum LA 1033 and identification of molecular markers显示文摘 | Rebeca C R Garbner R G | 2003 | Horticultural Science2003，35，3： | 1 |
| 4 | Genetic variation of Japanese encephalitis virus in nature显示文摘 | Chen W R Tesh Rt B Rebeca Rico-Hesse | 1990 | J Gen Virol1990，71，： | 1 |
| 5 | Effect of dietary carbohydrate-to-lipid ratios on growth,lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole (Solea senegalensis Kaup)显示文摘 | JORGE D REBECA R J STEPHAN P | 2004 | Aquaculture Research2004，35，12： | 1 |
| 6 | Nonlinear magic:multiphoton microscopy in the biosciences显示文摘 | Warren R Zipfel Rebeca M Williams Watt W Webb | | 0，，11： | 1 |
| 7 | Effect of dietary carbohydrate-to-lipid ratios on growth,lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole (Solea senegalensis Kaup)显示文摘 | JORGE D REBECA R J STEPHAN P | 2004 | Aquaculture Research2004，35，12： | 1 |
| 8 | Proximity to mining industry and eaneer mortality显示文摘 | Palo F N Javier G P Rebeca R | 2012 | Seienee of the Total Environment2012，435，1： | 1 |
| 9 | Performance Implications of Firm Resource Interactions in theAcquisition of R&D-Intensive Firms显示文摘 | DAVID R KING REBECA J SLOTEGRAAF IDALENE KESNER | 2008 | Organization Science2008，19，2： | 1 |
| 10 | Determinants of entrepreneurial intentions:Mexican immigrants in Chicago 显示文摘 | Rebeca R | 2001 | Journal of Socio-economics2001，，30： | 1 |
| 11 | PORPHOBILINOGEN DEAMINASE deficiency alters vegetative and reproductive development and causes lesions in arabidopsis显示文摘 | Victor Q Raquel S M Rebeca G B Andrea H Maria R P Jose L M | 2013 | PLoS ONE2013，8，53： | 1 |
| 12 | Effect of dietary carbohydrate-to-lipid ratios on growth,lipid deposition and metabolic hepatic enzymes in juvenile Senegalese sole(Solea senegalensis)显示文摘 | Jorge D Rebeca R J Stephan P | 2004 | Aquaculture Research2004，35，： | 1 |
| 13 | Determinants of entrepreneurial intentions: Mexican immigrants in Chicago 显示文摘 | Rebeca R | 2001 | Journal of Socio-Economics2001，，30： | 1 |
| 14 | Machine perfusion and the prevention of ischemic type biliary lesions following liver transplant:What is the evidence?显示文摘The widespread uptake of different machine perfusion(MP)strategies for liver transplant has been driven by an effort to minimize graft injury.Damage to the cholangiocytes during the liver donation,preservation,or early posttransplant period may result in stricturing of the biliary tree and inadequate biliary drainage.This problem continues to trouble clinicians,and may have catastrophic consequences for the graft and patient.Ischemic injury,as a result of compromised hepatic artery flow,is a well-known cause of biliary strictures,sepsis,and graft failure.However,very similar lesions can appear with a patent hepatic artery and these are known as ischemic type biliary lesions(ITBL)that are attributed to microcirculatory dysfunction rather than main hepatic arterial compromise.Both the warm and cold ischemic period duration appear to influence the onset of ITBL.All of the commonly used MP techniques deliver oxygen to the graft cells,and therefore may minimize the cholangiocyte injury and subsequently reduce the incidence of ITBL.As clinical experience and published evidence grows for these modalities,the impact they have on ITBL rates is important to consider.In this review,the evidence for the three commonly used MP strategies(abdominal normothermic regional perfusion[A-NRP],hypothermic oxygenated perfusion[HOPE],and normothermic machine perfusion[NMP])for ITBL prevention has been critically reviewed.Inconsistencies with ITBL definitions used in trials,coupled with variations in techniques of MP,make interpretation challenging.Overall,the evidence suggests that both HOPE and A-NRP prevent ITBL in donated after circulatory death grafts compared to cold storage.The evidence for ITBL prevention in donor after brain death grafts with any MP technique is weak. | Manuel Durán Rafael Calleja Angus Hann George Clarke Ruben Ciria Anisa Nutu Rebeca Sanabria-Mateos María Dolores Ayllón Pedro López-Cillero Hynek Mergental Javier Briceño M Thamara P R Perera | 2023 | World Journal of Gastroenterology2023，29，20： | 0 |
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