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| 1 | Chemical conjugation of a novel antibody interleukin 2 immunoconjugate against c erbB 2 product显示文摘Objective To develop a new chemical method to produce a monoclonal antibody (MoAb) 520C9/recombinant human interleukin 2 (rhIL 2) conjugate Methods MoAb 520C9 reactive with the protooncogene c erbB 2 product P185 was chemically conjugated with rhIL 2 by using a simple two step method First, the rhIL 2 was activated by Sulfosuccinimidyl 4 [N maleimidomethyl] cyclohexane 1 carboxylate, a heterobifunctional linker, and N succinimidyl s acetylthioacetate was introduced onto 520C9 Then SATA on the 520C9 was reacted with the maleimide group on the activated rhIL 2 to generate 520C9 rhIL 2 immunoconjugate Results The immunoconjugate retained the antigen binding activity compared to the respective native antibody as determined by an indirect live cell binding assay The immunoconjugate also possessed IL 2 activity as measured by the standard CTLL 2 cells proliferation assay and the stimulation of human peripheral blood mononuclear cells (PBMCs) into lymphokine activated killer cells Conclusion Our method of conjugation of rhIL 2 to 520C9 preserves the binding activity of the antibody and the cytokine function of IL 2 This simple and efficient method of conjugation should be applicable to other types of MoAbs and recombinant | 李君 Sharon E Lee Marie Belciug David B Ring Cheuk S Kwok | 2000 | Chinese Medical Journal2000，，2： | 3 |
| 2 | Total hepatectomy and liver transplantation as two-stage procedure显示文摘 | Ringe B Lubbe N Kuse E | 1993 | Ann Surg1993，218，1： | 1 |
| 3 | Alfacalcidol in men with osteoporosis: a prospective, observational, 2-year trial on 214 patients 显示文摘 | Ringe JD Farahmand P Schacht E | 2013 | Rheumatol Int2013，33，3： | 1 |
| 4 | Hepatoeyte-nuclear-factor-lalpha(HNF-lalpha), GATA-4 and caudal-related homeodomain protein Cdx2 functionally interact to modulate intestinal gene transcription:Implication for the developmental regulation of the sucrase-isomaltase gene 显示文摘 | Boudreau F Rings E H Wering H M V | 2002 | Journal of Biological Chemistry2002，，31： | 1 |
| 5 | Removing root canal obturation materials: a comparison of rotary file systems and re-treatment agents显示文摘 | Ring J Murray P E Namerow K N | 2009 | J Am Dent Assoc2009，140，6： | 1 |
| 6 | Selective glycogen synthase kinase 3 inhibitors potentiate insulin activation of glucose transport and utilization in vitro and in vivo 显示文摘 | RING D B JOHNSON K W HENRIKSEN E J | 2003 | Diabetes2003，52，3： | 1 |
| 7 | Innovative approa ches for the development of ntidepressant dugs:eurrent and fu ture strategies 显示文摘 | Schechter L E Ring R H Beyer C E | 2002 | NeuroRx2002，2，4： | 1 |
| 8 | The digestion and selective absorption of dietary fatty acids in Arctic charr,Salvelinus alpinus显示文摘 | Olsen R Henderson R Ring E | 1998 | Aquacult Nutr1998，，4： | 1 |
| 9 | Cytochrome P450 probe substrate metabolism kinetics in Sprague Dawley rats 显示文摘 | Chovan JP Ring SC Yu E | 2007 | Xenobiotica2007，37，： | 1 |
| 10 | Differential diagnosis of sclerosing cholangio-carcinoma of the common hepatic duct (Klatskin tumors) 显示文摘 | Wetter L A Ring E J Pellegrini C A | 1991 | Am J Surg1991，161，1： | 1 |
| 11 | Focus locol learning with wavelet networks显示文摘 | Ring E A Bilbro G L | 2002 | IEEE Trans on Neural Networks2002，13，2： | 1 |
| 12 | Potential of alfacalcidol for reducing increased risk of falls and fractures 显示文摘 | Ringe JD Schacht E | 2009 | Rheumatology International2009，29，10： | 1 |
| 13 | Potency of a combined alfacalcidol-alendronate therapy to reduce the risk of falls and fractures in elderly patients with glucocorticoid-induced osteoporosis显示文摘 | Ringe JD Schacht E Dukas L | 2011 | Arzneimittel Forschung2011，61，2： | 1 |
| 14 | Innovative approaches for lhe development of antidepressant drugs: current and future strategies显示文摘 | Schechter I E Ring R H Beyer C E | 2005 | NeuroRx2005，2，4： | 1 |
| 15 | Circulating tumour cells in breast cancer显示文摘 | RING A SMITH I E DOWSETT M | 2004 | Lancet Oncol2004，5，2： | 1 |
| 16 | Innovative approaches for the development of antidepressant drugs: current and future strategies显示文摘 | Schechter L E Ring R H Beyer C E | 2005 | NeuroRx2005，2，4： | 1 |
| 17 | Comparative metabolic capabilities of CYP3A4,CYP3A5, and CYP3A7显示文摘 | WILLIAMS J A RING B J CANTRELL V E | 2002 | Drug Metab Dispos2002，30，8： | 1 |
| 18 | In vitro toxicity of tetrabromobisphenol-A on cerebellar granule cells: cell death, free radical formation, calcium influx and extracetlular glutamate 显示文摘 | Reistad T Mariussen E Ring A | 2007 | Toxicol Sci2007，96，： | 1 |
| 19 | Lactic acid bacteria in fish: a review 显示文摘 | Ring E Gatesoupe F J | 1998 | Aquaculture1998，160，34： | 1 |
| 20 | Stress and secretory immunity显示文摘 | Bosch J A Ring C Geus de E J | | 0，，： | 1 |
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