|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Establishment of a novel corneal endothelial cell line from domestic rabbit,Oryctolagus curiculus显示文摘To develop a rabbit corneal endothelial (RCE) cell line, in vitro culture of RCE cells was initiated from Oryctolagus curiculus corneas and a novel RCE cell line was established in this study. To initiate the primary culture of RCE cells, corneas from rabbit eyes were sliced and attached into glutin-coated wells with endothelial cell surface down. After being cultured at a time-gradient interval from 48 to 6 h, the corneal slices were detached and reattached into new wells, respectively. Cells in the wells containing only a pure population of RCE cells were collected and cultured in 20% FBS-DMEM/F12 medium con- taining chondroitin sulfate, ocular extract, epidermal growth factor (EGF), basic fibroblast growth factor (bFGF), carboxymethyl-chitosan, N-acetylglucosamine hydrochloride, glucosamine hydrochloride, culture medium of rabbit corneal stromal cells and oxidation-degradation products of chondroitin sul- fate at 37℃, 5% CO2. The cultured RCE cells, in quadrangle and polygonal shapes, proliferated to con- fluence 3 weeks later. During the subsequent subculture, the shape of RCE cells changed gradually from polygonal to more fibroblastic. A novel RCE cell line, growing at a steady rate, with a population doubling time of 53.8 h, has been established and subcultured to passage 67. Chromosome analysis showed that the RCE cells exhibited chromosomal aneuploidy with the modal chromosome number of 44. The results of immuno-cytochemical staining with neuron specific enolase (NSE) confirmed that the RCE cells were in neuroectodermal origin. Combined with the results of vascular endothelial growth factor (VEGF) treatment and endothelial cell morphology recovery, it can be concluded that the cell line established here is an RCE cell line. This RCE cell line may serve as a useful tool in theoretical re- searches of mammalian corneal endothelial cells, and may also have potential application in artificial corneal endothelium development. | FAN TingJun, ZHAO Jun, FU YongFeng, CONG RiShan, GUO RuiChao, LIU WanShun, HAN BaoQin, YU QiuTao & WANG JingCollege of Marine Life Sciences, Division of Life Science and Technology, Ocean University of China, Qingdao 266003, China | 2007 | Science China(Life Sciences)2007，50，2： | 15 |
| 2 | Purification and characterization of phenoloxidase from Octopus ocellatus显示文摘从章鱼 ocellatus 的墨水囊的 Phenoloxidase (PO ) 被胶化过滤和离子交换层析净化,并且由把 L-dihydroxyphenylalanine (L-DOPA ) 用作特定的底层以它的生物化学、酶的性质描绘了。它从 O 被发现那 prophenoloxidase。当 153.8 kDa 的 heterodimeric 蛋白质,和 75.6 和 73.0 kDa 的二个子单元经常在 SDS 激活以后在准备被检测, ocellatus 被孤立。象 PO 一样活动显示出 7.0 的最佳的 pH, 40 的最佳的温度 ?????? | Tingjun Fan Mingyu Li Jing Wang Lingling Yang Rishan Cong | 2009 | Acta Biochimica et Biophysica Sinica2009，41，10： | 3 |
| 3 | Purification and characterization of hatching enzyme from brine shrimp Artemia salina显示文摘由把 Artemia 卵壳用作特定的底层,从 Artemia 盐咸滩(AHE ) 的孵化的酶被胶化过滤和离子交换层析净化,并且在这研究生物化学地并且酶地描绘了。AHE 由钠 dodecyl sulfatepolyacrylamide 胶化电气泳动有 82.2 kDa 的分子的重量,这被发现,并且经常在准备包含了 73.3 个 kDa 分子。AHE 举办了明显的 choriolytic 活动,哪个在 pH 是最佳的 7.0 并且 40 的温度 ?? 琠慲獮畤瑣潩 ???????????????????? ??????? ?? 僚吗?? | Tingjun Fan Jing Wang Wenpeng Yuan Qiwang Zhong Ying Shi Rishan Cong | 2010 | Acta Biochimica et Biophysica Sinica2010，42，2： | 2 |
| 4 | Pho- toelectrochemical property of Fe-N modified titania nano- tube array fihns显示文摘 | YU Lianqing LIU Rishan ZItANG Yaping | 2014 | Journal of Optoelectronics and Ad- vanced Materials2014，16，56： | 1 |
| 5 | Identification and characterization of a hemocyanin-derived phenoloxidase from the crab Charybdis japonica显示文摘 | Tingjun Fan Yanan Zhang Lingling Yang Xiuxia Yang Guojian Jiang Miaomiao Yu Rishan Cong | 2008 | Comparative Biochemistry and Physiology Part B2008，，2： | 1 |
| 6 | Effects of Several Immunostimulants on Phenoloxidase and Hemocytes of the Crab Charybdis japonica显示文摘To investigate the stimulating effects of immunostimulants on the autogenous immunocompetence of crabs and the possible mechanisms involved, the immunostimulating effects of β-1,3-glucan, lipopolysaccharide (LPS), inactivated Vibrio harveyi and Vibrio anguillarum on phenoloxidase (PO) and hemocytes of Charybdis japonica were investigated in this study. It was found that the yields and the enzymatic activities of purified PO in C. japonica increased significantly after the crabs were treated with immunostimulants, while the unit enzymatic activities remained almost the same. After treatment with β-1,3-glucan and LPS, the amount of rough endoplasmic reticulum (RER) and the number of mitochondria in both semigranular cells and granular cells increased greatly, and the number of cytoplasmic granules decreased but with enlarged volume. However, the corresponding characteristics of hyaline cells remained almost the same. On the other hand, the number of granules in semigranular cells decreased greatly, and the number of mitochondria of hyaline cells increased greatly, after treatment with inactivated vibrios. It may be concluded that the effect of polysaccharide immunostimulants on the innate immune system of C. japonica is different from that of inactivated vibrio immu-nostimulants. The immunity-enhancing mechanism of polysaccharides in crab autogenous immunocompetence is probably accomplished by the increased yields of PO and total PO activities, while that of inactivated vibrios is probably accomplished by the partially increased yields of PO and total PO activities as well as the significantly improved phagocytotic abilities of semigranular cells and hyaline cells. | FAN Tingjun YU Miaomiao YANG Lingling SHI Zhenping SUN Wenjie CONG Rishan YANG Xiuxia JIANG Guojian | 2009 | Journal of Ocean University of China2009，8，3： | 0 |
| 7 | The Catastrophe of Corruption in the Sustainability of Foreign aid:A Prediction of Artificial Neural Network Method in Indonesia显示文摘The rising corruption levels in Indonesia are becoming a cause for concern and raise doubts about their impact on the stability of foreign aid in the country.Therefore,this study aims to predict the long-term viability of foreign aid in Indonesia based on international perceptions of corruption and corruption cases in the country.Data were obtained from World Governance Indicators,the Indonesian Ministry of Finance,and the World Bank,and the study used a backpropagation artificial neural network(ANN)for prediction.The results from ANN are compared to linear models and vector autoregression(VAR).The finding shows that ANN outperforms the other models based on the coefficient of determination and MSE values.Furthermore,it highlights the strong relationship between corruption perception and foreign aid sustainability with an R-value of 0.991.According to the ANN estimation,gratification has a significant impact on foreign aid.In response to this finding,the study recommends the Indonesian government take action to combat corruption in maintaining the international trust and ensuring the stability of foreign aid. | Ade Paranata Rishan Adha Hoang Thi Phuong Thao Elin Erlina Sasanti Fafurida | 2023 | Fudan Journal of the Humanities and Social Sciences2023，16，2： | 0 |
| 8 | Characteristics of Farmer Tourism Market in Yanbian Korean Autonomous Prefecture显示文摘On the basis of comparison of urban and rural areas,distance and nationality,this paper analyzes characteristics of farmer tourism market in Yanbian Korean Autonomous Prefecture. There is a distinct difference in tourism of urban and rural residents. The tourism difference between outer suburban and inner suburban farmers is mainly manifested in organizational ways,travel distance,travel destination,and amount of consumption. Nationality difference is mainly shown in selection of means of transportation,length of travel time,and amount of consumption. | Rishan JIN Yanxiang LI Shizhu JIN | 2013 | Asian Agricultural Research2013，5，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员