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7篇 您的检索式：作者名="Ru Binggen"

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		题名	作者	年代	出处	被引量
	1	Comparative Proteome Analysis of Human Temporal Cortex Lobes by Two-Dimensional Electrophoresis and Identification of Selected Common Proteins显示文摘	Wen Chen Jianguo Ji Rui Zhao Binggen Ru	2002	Neurochemical Research2002，，9：	1
	2	Comparative Proteome Analysis of Human Temporal Cortex Lobes by Two-Dimensional Electrophoresis and Identification of Selected Common Proteins显示文摘	Wen Chen Jianguo Ji Rui Zhao Binggen Ru	2002	Neurochemical Research2002，，9：	1
	3	Purification and characterization of an sds-activated fibrinolytic enzyme from Eisenia fetida 显示文摘	Yang Jiashu Ru Binggen	1997	Comp Biochem Physiol1997，18，62：	1
	4	Comparative Proteome Analysis of Human Temporal Cortex Lobes by Two-Dimensional Electrophoresis and Identification of Selected Common Proteins显示文摘	Wen Chen Jianguo Ji Rui Zhao Binggen Ru	2002	Neurochemical Research2002，，9：	1
	5	Expression of the mouse metallothionein mutant ββ-cDNA in the lettuces (Lactuca sativa L.)显示文摘Mouse metallothionein (MT) domain mutant ββ-cDNA gene has been inserted into the plant expression vector pGPTVd35S which has herbicide (PPT)-resistance gene bar as a selective marker. The chimeric gene was introduced into the lettuce (Lactuca sativa L. cv Salinas 88) by Agrobacterium-mediated transformation. PCR and Southern blot analysis of some putative transformants indicated that the introduced ββ-cDNA was integrated into lettuce genome and inheritted by sexual reproduction. The expression of ββ gene in lettuce plants was demonstrated by Northern and Western blot analysis. Meanwhile, the zinc content of the transformed lettuce plants is up to 400 μg/g dry weight, remarkably higher than the control plants.	Zuo Xiaofeng Zhang Yukun Wu Beibei Chang Xing Ru Binggen	2002	Chinese Science Bulletin2002，47，7：	1
	6	Expression purification and characterization of p domain and p domain dimmer of metallothionein显示文摘	ZHOU Yuanjiao LI Lingyuan RU Binggen	2000	Biochimica et Biophysica Acta (BBA)-General Subjects2000，1524，2：	1
	7	Polycistronic strategy for cyanobacterial expression vector construction: Co-transcription of a human gene and a selective marker gene显示文摘A polycistronic expression vector, pKGA-NTF1, was constructed for the cyanobacterium. Within this vector, the spectinomycin/streptomycin resistance gene (aadA) facilitated the selection of transformants when co-transcribed with favorite genes. A natural glnA gene was selected as the platform to introduce the plasmid into a neutral site of the Synechococcus sp. PCC 7002 chromosome. Function of the vector was demonstrated by the insertion of a modified human Trefoil factor 3 gene (NTF1) to upstream of the aadA gene and by the analyses of the transformed strains. Antibiotics resistance assays showed that the dicistronic expression cassette conferred high spectinomycin resistance to both the E. coli cells and the Synechococcus cells. PCR analysis and Western-blot analysis were carried out to confirm the integration and expression of the NTF1 gene, respectively. Through simple molecular manipulations, the artificial polycistronic structure described here can be conveniently used to express other favorable genes or operons in cyanobacteria, and to study the cyanobacterial gene expression as well.	ZHANG Yukun SHI Dingji ZHAO Feifei YU Meimin RU Binggen	2005	Progress in Natural Science:Materials International2005，15，9：	0

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