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| 1 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 2 | Growth-inhibiting effects of taxol on human liver cancer in vitro and in nude mice显示文摘AIM To investigate the effects of taxol onSMMC-7721 human hepatoma and itsmechanisms.METHODS In vitro cell growth was assessedby trypan blue exclusion method.Experimentalhepatoma model was established by seedingSMMC-7721 cells subcutaneously into Balb/c(nu/nu)nude mice.In vivo tumor growth wasdetermined by measurement of tumor diameterwith Vernier calipers.The syntheses of DNA,RNA and protein were analyzed by incorporationof ~3H-thymidine,~3H-uridine and ~3H-leucinerespectively.Using light and electronmicroscopes to observe the morphologicalchanges of cells including mitosis andapoptosis.RESULTS Taxol was effective against SMMC-7721 human hepatoma cell growth in the rangesof 2.5 nmol/L-10 nmol/L with mitotic arrestand apoptosis in vitro.DNA,RNA and proteinsyntheses in cells were also obviouslysuppressed by in vitro treatment of taxol for72 h.Taxol at 2.5 nmol/L reduced ~3H-thymidineuptake to about 34% of the control value(P<0.05).Increasing the dose of taxol to20 nmol/L resulted in a greater decrease in ~3H-thymidine incorporation to 60% of the controlvalue(P<0.01).At a concentration of 20 nmol/L,the ~3H-uridine and ~3H-leucine uptakeswere reduced to 52%(P<0.05)and 63%(P<0.01),respectively.In vivo,taxolsignificantly inhibited SMMC-7721 tumor growthat 10 mg/kg,i.p.,once daily for 10 d.A morethan 90% decrease in tumor volume wasobserved by day 11(P<0.01)similarly withmitotic arrest and cell apoptosis.CONCLUSION Taxol has a marked anticanceractivity in SMMC-7721 human hepatoma both invitro and in nude mice.Its mechanisms might beassociated with mitotic arrest,subsequently,apoptosis of the hepatoma cells.No obvioustoxicity was observed with in vivoadministration of taxoi. | Jin Hui Yuan Ru Ping Zhang Ru Gang Zhang Li Xia Guo Xing Wang Wang Dan Luo Yong Xie Hong Xie | 2000 | World Journal of Gastroenterology2000，6，2： | 18 |
| 3 | The Predictive Value of Baseline HBs Ag Level and Early Response for HBs Ag Loss in Patients with HBe Ag-positive Chronic Hepatitis B during Pegylated Interferon Alpha-2a Treatment显示文摘Objective To explore the predictive value of baseline HBsAg level and early response for HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. Methods A total of 121 patients with HBeAg-positive chronic hepatitis B who achieved HBsAg loss were enrolled; all patients were treated with PEG-IFNα-2a 180 μg/week. Serum HBV DNA and serological indicators(HBsAg, anti-HBs, HBeAg, and anti-HBe) were determined before and every 3 months during treatment. Results The median treatment time for HBsAg loss was 84 weeks(7-273 weeks), and 74.38%(90 cases) of the patients needed extended treatment(> 48 weeks). The correlation between baseline HBsAg levels and the treatment time of HBsAg loss was significant(B = 14.465, t = 2.342, P = 0.021). Baseline HBsAg levels together with the decline range of HBsAg at 24 weeks significantly correlated with the treatment time of HBsAg loss(B = 29.862, t = 4.890, P = 0.000 and B = 27.993, t = 27.993, P = 0.005). Conclusion Baseline HBsAg levels and extended therapy are critical steps toward HBsAg loss. Baseline HBsAg levels together with early response determined the treatment time of HBsAg loss in patients with HBeAg-positive chronic hepatitis B during pegylated interferon alpha-2a treatment. | LI Ming Hui ZHANG Lu QU Xiao Jing LU Yao SHEN Ge LI Zhen Zhen WU Shu Ling LIU Ru Yu CHANG Min HU Lei Ping HUA Wen Hao SONG Shu Jing WAN Gang XIE Yao | 2017 | Biomedical and Environmental Sciences2017，30，3： | 14 |
| 4 | Using a non-radioisotopic, quantitative TRAP-based method detecting telomerase activities in human hepatoma cells显示文摘A non-radioisotopic, quantitative TRAP-based telomerase activity assay was established mainly by using SYBR Green-I staining instead of radioisotope. Comparing with conventional radioisotope based method, it was better in reproducibility and accuracy. Using this method, we found telomerase activities were absent in normal human liver cells, while detected in ail of four human hepatoma cell lines (BEL-7404, SMMC-7721, QGY-7903 and HCCM) without significant differences. | ZHANG RU GANG XING WANG WANG JIN HUI YUAN LI XIA GUO HONG XIE (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China) | 2000 | Cell Research2000，10，1： | 8 |
| 5 | Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells显示文摘Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 1uM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G2/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process. | Ru GANG ZHANG, Ru PING ZHANG, XING WANG WANG, HONG XIE Department of Biotherapy, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China | 2002 | Cell Research2002，12，1： | 3 |
| 6 | Catalytic hydrolysis of phosphate diester (BNPP) and plasmid DNA by mononuclear macrocyclic polyamine metal complexes显示文摘磷酸盐 diest 的催化水解作用的活动嗯(BNPP )[ 二度(p-nitrophenyl ) 磷酸盐 diest 嗯] 并且由单音的原子宏的原生质标志 DNA (pUC18 ) 周期的 polyamine 金属建筑群在这篇论文被调查了。结果证明在 BNPP 的水解作用的最高的活动与 1e – 被获得; Zn (II ) 建筑群(脂性的组镇静) 是催化剂。水解作用率改进直到 3.64 × 10 4 褶层。这些金属建筑群能有效地以生理的条件支持原生质标志 DNA (pUC18 ) 的劈开。 | Qing Xiang Xiang Li Qun Zhang Xiao Qi Yu Ru Gang Xie | 2009 | Chinese Chemical Letters2009，20，5： | 3 |
| 7 | Synthesis of Imidazole Cyclophane as Ligand of Binuclear Metalloprotein Models显示文摘Fotir Anidazole cydophanes have been srnthesised by the oxidation ofbis-imidazoline with banum manganate followed by theation with 2,6-bis(bromomethyl) pwidine in the presence of NaH. All the compotmds were identilied byIR, 1HNMR, MS and elemental analvsis. | Jia Ming YAN Zong Lin JIANG Wen Qi LIU Ru Gang XIE(Department of Chemistry. Sichuan University, Chengdu, Sichuan 610064)(Department of Chemistry, Daxian Teachers College. Daxian. Sichuan 635000) | 1997 | Chinese Chemical Letters1997，8，5： | 1 |
| 8 | A New Synthesis of 1-(ω-Haloalkyl)-Imidazoles显示文摘A new synthesis of 1 - (ω- haloalkyl ) -imidazole has been developed. The synthesisinvolves a Hoffmann-type elimination of the 2-cyanoethyl group from inddazolium salts obtainedby the reaction of α,ω-dihalide with 1-(2-cyanoethyl)-imidazole. | Jia Ming YAN Zong Lin JLANG Lin ZHANG Ru Gang XIE(Department of Chendstry, Sichuan University, Chengdu 610064)(Department of Chendstry, Daxian Teachers’ College, Daxian 635000) | 1998 | Chinese Chemical Letters1998，9，6： | 1 |
| 9 | Novel Bis-hydroxymethylation of Imidazole and 2-Methylimidazole显示文摘A noxel one-pot synthesis of 4,5-bis(hydrormethy) imidazoles 2a-b, new ethers 3a-b and a new type of imidazolophane 4b by the bis-hydroxymethylation of imidazole or 2-methyl imidazole with formaldehyde and potassium hydroxide is reported. The structures of these compounds have been established by MS, 1HNMR IR and elemental analyses. | Cheng He ZHOU Zhi Chang LIU Ru Gang XIE Hua Ming ZHAO(Department of Chemistry, Faculty of Science, Sichuan Union University, Chengdu 610064) | 1997 | Chinese Chemical Letters1997，8，1： | 0 |
| 10 | Catalytic Hydrolysis of Lipophilic Amino Acid Esters by Metallomicelles as Carboxypeptidase A Models显示文摘Lipophilic complexes of macrocyclic 12-membered dioxotetrartne ligand bearing bishydroxy groups as fiJnctional pendants are effective catalysts for the hydrolysis of amino acid esters in ndcellar media, and the rate enhancement of more than lo-fold were observed. | Xiao Qi YU Jing Song YOU Qing Xiang XIANG Qian Shun YAN Ru Gang XIE (Department of Chemistry, Sichuan University, Chengdu 610064) | 1998 | Chinese Chemical Letters1998，9，8： | 0 |
| 11 | The Synthesis of New Sulfur-containing Chiral Macrocyclic Ligands显示文摘Five new chiral sulfur-containing macrocyclic ligands which can be used in chiral recognition to guests have been synthesized and | Xiao Ling ZHANG Jing Song YOU Xing Shu LI Ru Gang XIE(Department of Chemistry, Sichuan Union University, Chengdu 610064) | 1997 | Chinese Chemical Letters1997，8，10： | 0 |
| 12 | Synthesis of Bis-N-squaramidoacids and their Applications to Asymmetric Reduction of Prochiral Ketone and Diketones显示文摘A series of novel bis-squaramidoacid ligands were prepared. Prochiral ketone and diketones were direct reduced by borane in the presence of these ligands giving secondary alcohol products with enantiomeric excesses up to 64.2% for w-bromoacetophenone and 90.0% for 1, 6-diphenyl-1, 6-hexanedione. | Lei Lei LI, Ji ZHANG, Ru Gang XIE* Department of Chemistry, Sichuan University, Chengdu 610064 | 2003 | Chinese Chemical Letters2003，14，7： | 0 |
| 13 | A Facile and Efficient synthesis for A Series of Bis(N-imidazolylethyl) Ethers显示文摘A facile and efficient synthesis for a series of bis(N-imidazolylethyl) ethers from oligo-ethyleneglycols and imidazole has been studied. All these compounds obtained were characterized by MS, HNMR, and elemental analysis. | Cheng He ZHOU Yu Lan WANG Ru Gang XIE Meng Shen CAI (Department of Bioorganic Chemistry, State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083)(Department of Chemistry, Schoo | 1998 | Chinese Chemical Letters1998，9，7： | 0 |
| 14 | DESIGN AND SYNTHESIS OF CYCLODEXTRIN DIMERS WITH TWO IMIDAZOLIUM RESIDUES AS CATALYTIC SITE显示文摘DESIGNANDSYNTHESISOFCYCLODEXTRINDIMERSWITHTWOIMIDAZOLIUMRESIDUESASCATALYTICSITEDESIGNANDSYNTHESISOFCYCLODEXTRINDIMERSWITHTWOI... | Sheng Jin GUO Mei Ming LUO Xiao Rong GU Ru Gang XIE Hua Ming ZHAO(Department of Chemistry, Sichuan University, Chengdu, 610064) | 1995 | Chinese Chemical Letters1995，6，4： | 0 |
| 15 | Enantioselective Hydrolysis of α-Amino Acid Ester by Chiral Metallomicelles显示文摘A series of chiral lipophilic Cu(Ⅱ) complexes were investigated as catalysts for the enantioselective hydrolysis of R(S)-p-nitrophenyl N-dodecanoyl-phenylalaninate in micelles. The highest enantioselectivity (kR/ks=7. 81) was observed in a mixed micellar system composed of 1-Cu(Ⅱ) and Brij35. | Jing Song YOU Xiao Qi YU Xiao Ling ZHANG and Ru Gang XIE(Department of Chemistry, Sichuan University, Chengdu 610064) | 1998 | Chinese Chemical Letters1998，9，4： | 0 |
| 16 | Synthesis of Novel Cyclodextrin Dimers Linked by Multidentate Coordination Units显示文摘A new type of cyclodextrin dimers (2a-c) bridged with multidentate coordination unitshas been synthesized by treatment of 6 -deoxy-6-hydroxyethylamino- β- cyclodextrin l with corre-sponding dibromides in the presence of base. | Mei Ming LUO Ru Gang XIE Wei LU ping Fang XLA Hua Ming ZHAO (Department of Chemistry, Sichuan University, Chengdu 610064Department of Chemistry, Sichuan Educational College, Chengdu 610041) | 1998 | Chinese Chemical Letters1998，9，2： | 0 |
| 17 | The complexes of multiimidazole with copper(Ⅰ) display tyrosinase activity显示文摘The six multiimidazole copper(Ⅰ)complexes are utilized to mimic tyrosinase.The main product is 3,5-di-t-butylcatechol from o-hydroxylation of the substrate 2,4-di-t-butylphenol.The highest yield of catechol is up to 82.2% and selectivity 94.8% by [Cu(I)_(3b)(MeCN)_2](ClO_4)_2 and O_2 under mild conditions,which are found to be more efficient than that already reported. | Rong Xiao Xiao Yu Su Ru Gang Xie | 2007 | Chinese Chemical Letters2007，18，10： | 0 |
| 18 | Enantioselective Reduction of Prochiral Ketones Catalyed by Oxazaborolidine Derived from L-Cystine显示文摘Two new chiral oxazaborolidine derivated from L-cystine have been used to catalyze the enantioselective reduction of prochiral ketones and the secondary alcohols are obtained with good to excellent opitical yields. | Xing Shu Li Xiao Ling Zhang Ru Gang Xie(Department of Chemistry, Sichuan Union University, Chengdu, 610064)(Department of Chemistry, Sichuan Educational College, Chengdu, 610041) | 1997 | Chinese Chemical Letters1997，8，8： | 0 |
| 19 | Ortho-Hydroxylation of Phenol by Tyrosinase Model Compounds显示文摘A series of tyrosinase model ligands and complexes containing polyimidazoles wereprepared. 2, 4-Di-tert-butyl-phenol was ortho-hydroxylated by the binuclear copper (?) complex[Cu2(6a)(CH3CN)2](ClO4)2 8a and molecular dioxygen under mild conditions with up to 80.4%yield, 91.4% selectivity and 92.0% conversion. | Dai Rong YUAN Jia Ming YAN Cheng Zhi YU Ru Gang XIE | 2005 | Chinese Chemical Letters2005，16，2： | 0 |
| 20 | FUNCTIONALIZED CYCLODEXSTRINS BEARING AM INOALKYLIMINO GROUPS AS ACTIVE CENTERS TO CATALYZE ALDOL CONDENSATIONS显示文摘Efficient catalysis of functinnatized β-cyctodextrins bearing aninoatkytimino groups for atdot condensations of nitrobenzatdehydes and acetone has been effected and substantiated by preparative | De Qi YUAN~(**), Ru Gang XIE, Hua Ming ZHAO (Department of Chemistry, Sichuan University, Chengdu, Sichuan 610054) | 1991 | Chinese Chemical Letters1991，2，8： | 0 |
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